Expression Pattern of Aquaporin 1 and Aquaporin 3 in Melanocytic and Nonmelanocytic Skin Tumors

Abstract Objectives Study of aquaporin 1 (AQP1) and aquaporin 3 (AQP3) expression to understand its potential role in the pathophysiology of skin cancer. Methods Analysis of AQP1 and AQP3 expression by immunohistochemistry of 72 skin biopsy specimens from melanocytic skin tumors, nonmelanocytic tumors, or healthy samples. Results AQP1 showed strong labeling in 100% of benign common melanocytic nevi. Small blood vessels, stroma, and melanophages surrounding different types of melanomas tumors also were positive. Tumoral melanocytes in atypical nevi and melanomas were negative for AQP1. AQP3 showed strong labeling in 100% of melanocytic nevi, 100% of atypical melanocytic nevi, and 100% of melanomas. In all basal cell carcinomas and squamous cell carcinomas, staining for AQP3 was positive. Conclusions To our knowledge, this work represents the first demonstration of AQP1/AQP3 expression in human melanocytic skin tumors. More studies are needed to understand the underlying molecular mechanisms of expression of both AQPs in melanocytic tumors and their potential as molecular therapeutic targets.

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Silencing of LINC00707 suppresses cell proliferation, migration, and invasion of osteosarcoma cells by modulating miR-338-3p/AHSA1 axis

Open Life Sciences ◽

10.1515/biol-2021-0070 ◽

2021 ◽

Vol 16 (1) ◽

pp. 728-736

Author(s):

Xiao-rong Zhang ◽

Jian-li Shao ◽

Heng Li ◽

Liang Wang

Keyword(s):

Cell Proliferation ◽

Cell Lines ◽

Molecular Mechanisms ◽

Migration And Invasion ◽

Osteoblastic Cell ◽

Osteosarcoma Cell ◽

Osteoblastic Cell Line ◽

Molecular Therapeutic ◽

Molecular Therapeutic Targets ◽

Competitive Endogenous Rna

Abstract Osteosarcoma is the most common type of primary malignant tumor of the bone, with a high metastatic rate and poor prognosis. Therefore, it is important to further elucidate the molecular mechanisms involved in the development of osteosarcoma and explore new molecular therapeutic targets. Long intergenic nonprotein-coding RNA 707 (LINC00707) is an oncogenic gene in several cancers. In this study, we further clarified its role and regulatory mechanism in osteosarcoma. We found that LINC00707 levels are significantly higher in the osteosarcoma cell lines SW 1353, HOS, U-2 OS, MG-63, and Saos-2 compared to those in human fetal osteoblastic cell line hFOB1.19. LINC00707 silencing suppressed cell proliferation, migration, and invasion of MG-63 and Saos-2 cells. Moreover, LINC00707 can act as a competitive endogenous RNA of miR-338-3p, and miR-338-3p inhibitor and AHSA1 overexpression alleviated the effect of LINC00707 silencing. In conclusion, we demonstrated high expression of LINC00707 in osteosarcoma cell lines and that silencing LINC00707 suppresses cell proliferation, migration, and invasion by targeting the miR-338-3p/AHSA1 axis in MG-63 and Saos-2 cells. These findings suggest that LINC00707 may serve as a potential target for osteosarcoma treatment.

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Modified Dermoscopic Algorithm for the Differentiation between Melanocytic and Nonmelanocytic Skin Tumors

Journal of Cutaneous Medicine and Surgery ◽

10.2310/7750.2006.00021 ◽

2006 ◽

Vol 10 (2) ◽

pp. 73-78 ◽

Cited By ~ 3

Author(s):

Andreas Blum ◽

Jennifer Clemens ◽

Giuseppe Argenziano

Keyword(s):

Diagnostic Accuracy ◽

Basal Cell ◽

Skin Lesions ◽

Skin Tumors ◽

Melanocytic Tumors ◽

Melanocytic Tumor ◽

Clinical Diagnostic ◽

Basal Cell Carcinomas ◽

Surface Microscopy

Background: The use of dermoscopy (dermatoscopy, epiluminescence microscopy, surface microscopy) improves the clinical diagnostic accuracy of skin tumors by applying different algorithms or scores. The first step in the dermoscopic evaluation is the differentiation between melanocytic and nonmelanocytic skin tumors. Objective: To evaluate the diagnostic accuracy of the established dermoscopic algorithm (EDA) and the modified dermoscopic algorithm (MDA) for melanocytic versus nonmelanocytic skin tumors. Methods: Two hundred forty-nine patients with melanocytic and nonmelanocytic skin lesions were included. Dermoscopic images of the tumors were taken with 10-fold magnification, followed by surgery and histopathology at the departments of Dermatology at the universities of Tuebingen, in Germany, and Naples, in Italy. Each lesion was classified using the EDA and MDA. In the MDA, accessory nipples and dermatofibromas were considered in particular. Results: With the EDA, 225 of 249 (90.4%) skin tumors were correctly classified in one of the six groups. With the MDA, 237 of 249 (95.2%) were correctly classified. Improvement was achieved in 12 (4.8%) better classified skin tumors. In both algorithms, no melanoma was classified as a nonmalignant melanocytic tumor. All melanomas were classified in the group of melanocytic tumors and one melanoma was classified in the group of basal cell carcinomas. Conclusion: Both dermoscopic algorithms for the differentiation between melanocytic and nonmelanocytic skin tumors were simple and effective when applied step by step. The MDA is an improvement on the EDA with the classification of accessory nipples and dermatofibromas.

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CYTOLOGICAL DIAGNOSIS AS A METHOD OF SCREENING OF PRE-MALIGNANT MELANOCYTIC TUMORS AND EARLY FORMS OF SKIN MELANOMA

Problems in oncology ◽

10.37469/0507-3758-2018-64-3-384-387 ◽

2018 ◽

Vol 64 (3) ◽

pp. 384-387

Author(s):

Viktor Novik ◽

D. Dreval

Keyword(s):

Laser Therapy ◽

Histological Examination ◽

Clinical Data ◽

Surgical Excision ◽

Cytological Diagnosis ◽

Skin Melanoma ◽

Cytological Examination ◽

Melanocytic Tumors ◽

Quality Character ◽

Atypical Nevi

Cytohistological comparisons to the account of the clinical data and revision of cytological and histological preparations on a material received from 21 patients are made. Cytomorphological features of juvenile nevi (Spitz-nevus, Reed-nevus), dysplastic and atypical nevi and early forms of melanoma are described. The establishment at cytological examination of good-quality character of melanocytic defeats at the account of the clinical data could be the basis for appointment laser therapy. At revealing of atypical melanocytes in cytological preparations patients should be referred to specialized oncological institutions for surgical excision of tumor with the subsequent histological examination. Thus cytological examination could be used in dermatological practice as a method of screening pre-malignant melanocytic tumors and skin melanoma.

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Identifying the Molecular Cause of Extreme Endoplasmic Reticulum Dilation in Pediatric Osteosarcoma and Its Relationship to the Disease

10.21007/etd.cghs.2021.0555 ◽

2021 ◽

Author(s):

◽

Rachael Wood ◽

Keyword(s):

Endoplasmic Reticulum ◽

Genomic Instability ◽

Cell Lines ◽

Molecular Signature ◽

Osteosarcoma Cell ◽

Copii Vesicle ◽

Molecular Therapeutic ◽

Molecular Therapeutic Targets ◽

Pediatric Osteosarcoma ◽

Crispr Activation

Pediatric osteosarcoma tumors are characterized by an unusual abundance of grossly dilated endoplasmic reticulum and an immense genomic instability that has complicated identifying new effective molecular therapeutic targets. Here we report a novel molecular signature that encompasses the majority of 108 patient tumor samples, PDXs and osteosarcoma cell lines. These tumors exhibit reduced expression of four critical COPII vesicle proteins that has resulted in the accumulation of procollagen-I protein within ‘hallmark’ dilated ER. Using CRISPR activation technology, increased expression of only SAR1A and SEC24D to physiologically normal levels was sufficient to restore both collagen-I secretion and resolve dilated ER morphology to normal.

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KIF15 Promotes Proliferation and Growth of Hepatocellular Carcinoma

Analytical Cellular Pathology ◽

10.1155/2020/6403012 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Yue-Feng Sun ◽

Hong-Li Wu ◽

Rui-Fang Shi ◽

Lin Chen ◽

Chao Meng

Keyword(s):

Hepatocellular Carcinoma ◽

Liver Cancer ◽

Critical Role ◽

Great Promise ◽

Cell Processes ◽

Multiple Cell ◽

Molecular Therapeutic ◽

Molecular Therapeutic Targets

Liver cancer is thought as the most common human malignancy worldwide, and hepatocellular carcinoma (HCC) accounts for nearly 90% liver cancer. Due to its poor early diagnosis and limited treatment, HCC has therefore become the most lethal malignant cancers in the world. Recently, molecular targeted therapies showed great promise in the treatment of HCC, and novel molecular therapeutic targets is urgently needed. KIF15 is a microtubule-dependent motor protein involved in multiple cell processes, such as cell division. Additionally, KIF15 has been reported to participate in the growth of various types of tumors; however, the relation between KIF15 and HCC is unclear. Herein, our study investigated the possible role of KIF15 on the progression of HCC and found that KIF15 has high expression in tumor samples from HCC patients. KIF15 could play a critical role in the regulation of cell proliferation of HCC, which was proved by in vitro and in vivo assays. In conclusion, this study confirmed that KIF15 could be a novel therapeutic target for the treatment of HCC.

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