Prognostic significance of tumour tissue NeuGcGM3 ganglioside expression and predictive value of circulating tumour cell count monitoring in patients receiving racotumomab immunotherapy

IntroductionSplenectomy-induced thrombocytosis and leukocytosis may obscure the early diagnosis of post-operative infection or sepsis. In trauma patients after splenectomy, a platelet-to-white blood cell ratio of <20 has been shown to reliably differentiate post-operative sepsis from transient physiological responses.ObjectiveTo determine whether the platelet-to-white blood cell ratio can be applied to differentiate between reactive post-operative changes and latent infection.MethodsAll consecutive patients with ovarian cancer who underwent splenectomy between January 2013 and October 2018 in two large European gynecological cancer centers were retrospectively evaluated. Main outcome measures were white blood cell count, platelet count, and platelet-to-white blood cell ratio on post-operative days 1, 5, and 7. These were correlated with surgical outcome and morbidity according to the Clavien-Dindo classification. A binomial logistic regression was applied to assess the predictive value of day 5 platelet-to-white blood cell ratio, white blood cell count, and platelet count for predicting grade III post-operative sepsis.ResultsNinety-five patients with ovarian cancer (mean age 54 years, range 18–75) were identified. Seventeen patients (17.9%) developed a grade III post-operative sepsis. In all post-operative patients, mean white blood cell count on day 5 decreased (from 15.4×103/μL to 11.4×103/μL), while the mean platelet count rose (from 260.7×103/μL to 385.3×103/μL). A high platelet count (>313×103/μL) failed to show any predictive value (OR=0.94; 95% CI 0.30 to 3.0; p=0.921). A low platelet-to-white blood cell ratio (<26) (OR=3.49; 95% CI 1.18 to 10.32; p=0.0241) and high white blood cell count (>14.5×103/μL) on day 5 (OR=11.0; 95% CI 3.3 to 36.2; p<0.001) were significant for predicting sepsis. Despite a significant OR, the sensitivity and specificity were low; day 5 platelet-to-white blood cell ratio at a cut-off point of 26 achieved a sensitivity of 72% and specificity of 53% (area under the curve 0.637, 95% CI 0.480 to 0.796) in predicting grade III post-operative sepsis.ConclusionsPlatelet-to-white blood cell ratio after cytoreductive surgery for ovarian cancer with splenectomy does not appear to have a strong predictive value in differentiating between sepsis and reactive splenectomy-induced changes. Leukocytosis, in combination with clinical assessment, may remain the most useful tool for prediction of sepsis after cytoreductive surgery with splenectomy.

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27: Diagnostic utility of unbiased circulating-tumour cell capture through negative depletion of peripheral blood cells

Lung Cancer ◽

10.1016/s0169-5002(15)50027-5 ◽

2015 ◽

Vol 87 ◽

pp. S10-S11

Author(s):

D.V. Freydin ◽

D. Chudasama ◽

M.B. Freidin ◽

M. Leung ◽

D.N. Butcher ◽

...

Keyword(s):

Tumour Cell ◽

Peripheral Blood ◽

Blood Cells ◽

Diagnostic Utility ◽

Peripheral Blood Cells ◽

Cell Capture ◽

Circulating Tumour Cell

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Circulating tumour cell PD-L1 test for head and neck cancers

Oral Oncology ◽

10.1016/j.oraloncology.2017.10.011 ◽

2017 ◽

Vol 75 ◽

pp. 6-7 ◽

Cited By ~ 6

Author(s):

Arutha Kulasinghe ◽

Liz Kenny ◽

Chamindie Punyadeera

Keyword(s):

Head And Neck ◽

Tumour Cell ◽

Head And Neck Cancers ◽

Circulating Tumour Cell

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Development of a low-cost magnetic microfluidic chip for circulating tumour cell capture

IET Nanobiotechnology ◽

10.1049/iet-nbt.2011.0024 ◽

2011 ◽

Vol 5 (4) ◽

pp. 114-120 ◽

Cited By ~ 9

Author(s):

J. Xia ◽

C.Z. Zhou ◽

Y.G. Li ◽

X. Chen ◽

Z.H. Peng

Keyword(s):

Microfluidic Chip ◽

Tumour Cell ◽

Low Cost ◽

Cell Capture ◽

Circulating Tumour Cell

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Predictive Value of Interim PET/CT in DLBCL Treated with R-CHOP: Meta-Analysis

BioMed Research International ◽

10.1155/2015/648572 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 11

Author(s):

Na Sun ◽

Jinhua Zhao ◽

Wenli Qiao ◽

Taisong Wang

Keyword(s):

Likelihood Ratio ◽

Predictive Value ◽

Meta Analysis ◽

Prognostic Significance ◽

Positive Likelihood Ratio ◽

Negative Likelihood Ratio ◽

Chop Chemotherapy ◽

Interim Pet ◽

Pet Ct ◽

Fdg Pet Ct

Objective.We conducted a meta-analysis to evaluate the predictive value of interim18F-FDG PET/CT in patients with DLBCL treated with R-CHOP chemotherapy.Methods.We searched for articles published in PubMed, ScienceDirect, Wiley, Scopus, and Ovid database from inception to March 2014. Articles related to interim PET/CT in patients with DLBCL treated with R-CHOP chemotherapy were selected. PFS with or without OS was chosen as the endpoint to evaluate the prognostic significance of interim PET/CT.Results.Six studies with a total of 605 cases were included. The sensitivity of interim PET/CT ranged from 21.2% to 89.7%, and the pooled sensitivity was 52.4%. The specificity of interim PET/CT ranged from 37.4% to 90.7%, and the pooled specificity was 67.8%. The pooled positive likelihood ratio and negative likelihood ratio were 1.780 and 0.706, respectively. The explained AUC was 0.6978 and theQ*was 0.6519.Conclusions.The sensitivity and specificity of interim PET/CT in predicting the outcome of DLBCL patients treated with R-CHOP chemotherapy were not satisfactory (52.4% and 67.8%, resp.). To improve this, some more work should be done to unify the response criteria and some more research to assess the prognostic value of interim PET/CT with semiquantitative analysis.

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PB2344 EVALUATION OF THE PREDICTIVE VALUE OF HEMATOPOIETIC PROGENITOR CELL COUNT IN SYSMEX XN ANALYZERS FOR CD34+ STEM CELL MOBILIZATION IN PERIPHERAL BLOOD PREVIOUS TO THE APHERESIS

HemaSphere ◽

10.1097/01.hs9.0000567840.67834.1e ◽

2019 ◽

Vol 3 (S1) ◽

pp. 1044

Author(s):

J. R. Furundarena ◽

J. J. Ferreiro ◽

A. Alkorta ◽

C. González ◽

A. Uranga ◽

...

Keyword(s):

Stem Cell ◽

Cell Count ◽

Peripheral Blood ◽

Predictive Value ◽

Progenitor Cell ◽

Stem Cell Mobilization ◽

Hematopoietic Progenitor Cell ◽

Hematopoietic Progenitor ◽

Cell Mobilization

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660 POSTER Toward a circulating tumour cell analysis as an early marker for relapse in stage II and III colorectal cancer patients

European Journal of Cancer Supplements ◽

10.1016/s1359-6349(05)80956-5 ◽

2005 ◽

Vol 3 (2) ◽

pp. 187

Keyword(s):

Colorectal Cancer ◽

Cancer Patients ◽

Tumour Cell ◽

Stage Ii ◽

Cell Analysis ◽

Early Marker ◽

Circulating Tumour Cell ◽

Colorectal Cancer Patients

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Tumor-associated immune cells and progression-free survival in advanced endometrial cancer (EC), results from the PHAEDRA trial (ANZGOG 1601).

Journal of Clinical Oncology ◽

10.1200/jco.2021.39.15_suppl.5584 ◽

2021 ◽

Vol 39 (15_suppl) ◽

pp. 5584-5584

Author(s):

Deborah Smith ◽

Kristy Robledo ◽

Sonia Yip ◽

Michelle Cummins ◽

Peey-Sei Kok ◽

...

Keyword(s):

Immune Cells ◽

Predictive Value ◽

Tumor Response ◽

External Validation ◽

Prognostic Significance ◽

Progression Free Survival ◽

Immune Biomarkers ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

The Individual

5584 Background: Activity of durvalumab in patients with deficient mismatch repair (dMMR) advanced endometrial carcinoma (EC) was confirmed in the PHAEDRA trial (ANZGOG 1601). This study investigated the association between immune biomarkers and clinical outcomes in PHAEDRA. Methods: Formalin-fixed paraffin embedded sections immunohistochemically stained for PD-L1 using the Ventana platform, were with matched H&E slides scored independently by two pathologists according to the Ventana PD-L1 (SP263) algorithm for urothelial carcinoma (UC). Immune biomarkers assessed were PD-L1 staining of tumor cells (TCP) and immune cells (IC), and presence of tumor-associated immune cells (ICP). Results: Sixty-seven of the 71 patients had sufficient tumor for PD-L1 testing. AUC were 0.667, 0.726 and 0.644 for TCP, ICP and IC, respectively for predicting tumor response. Optimal cutpoints were TCP≥1%, ICP≥10% and IC≥35%. ICP≥10% achieved the highest sensitivity (53%) and specificity (82%) of the individual cutpoints. The optimal cutpoint algorithm was able to identify patients who would not respond, (sensitivity 88%, negative predictive value 92%), but had low specificity (48%) and positive predictive value (37%). Differences in PFS were found using ICP≥10% (logrank p = 0.01), compared to TCP (p = 0.25), IC (p = 0.48) and the UC algorithm (p = 0.08) (Figure 1). PFS was shorter in patients with pMMR than dMMR after adjusting for ICP (HR 2.99, 95%CI: 1.61-5.57, p < 0.001). Adjustment for MMR reduced the prognostic significance of ICP≥10% for PFS (HR 0.59, 95% CI: 0.28-1.23, p = 0.16). For OS, differences were seen for the UC algorithm (p = 0.02), but not ICP (p = 0.07), TCP (p = 0.18) or IC (p = 0.23). Similarly to PFS, adjustment for MMR reduced the prognostic significance of the UC algorithm for OS (HR: 0.53, 95% CI: 0.25-1.12, p = 0.10). Conclusions: In this exploratory analysis, ICP was more closely associated with tumor response and PFS than TCP or IC. ICP alone was better than the UC algorithm for predicting PFS. The optimum cutpoint algorithm was promising for identifying non-responders, but requires external validation. Clinical trial information: ACTRN12617000106336.

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