Orange peel extract reduces the inflammatory state of skeletal muscle after downhill running via an increase in IL-1RA

Abstract It has been reported that orange peel extract (OPE) and the four major polymethoxyflavones (PMFs) in OPE have a protective effect against downhill running (DR)-induced skeletal muscle inflammation. However, the mechanism is not well understood. We investigated the potential of OPE and PMF compounds for increasing anti-inflammatory cytokine levels. The plasma interleukin-1 receptor antagonist (IL-1RA) level was increased 1 and 8 h after OPE administration in rats. Nobiletin induced the secretion of IL-1RA from C2C12 myotubes. In the inflammatory state of skeletal muscle after DR, OPE administration reduced nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) expression, NF-κB-DNA binding, and monocyte chemotactic protein-1 mRNA levels, but these effects were all abrogated by the intravenous administration of IL-1RA neutralizing antibody. These results indicated that OPE reduces skeletal muscle inflammatory state after DR via an increase in IL-1RA, and that IL-1 receptor signaling is important for skeletal muscle inflammation after DR.

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Polymethoxyflavones in orange peel extract prevent skeletal muscle damage induced by eccentric exercise in rats

Bioscience Biotechnology and Biochemistry ◽

10.1093/bbb/zbaa036 ◽

2020 ◽

Vol 85 (2) ◽

pp. 440-446

Author(s):

Toshihide Suzuki ◽

Makoto Shimizu ◽

Yoshio Yamauchi ◽

Ryuichiro Sato

Keyword(s):

Skeletal Muscle ◽

Muscle Damage ◽

Eccentric Exercise ◽

Vastus Lateralis ◽

Orange Peel ◽

Skeletal Muscle Damage ◽

Downhill Running ◽

Exercise Induced ◽

Orange Peel Extract ◽

Peel Extract

ABSTRACT Polymethoxyflavones (PMFs) contained in the peel of citrus fruits have anti-inflammatory, anticancer, and antidepressant effects. However, their effects on skeletal muscle are unknown. We investigated whether PMFs could prevent skeletal muscle damage induced by eccentric exercise in rats. Downhill running for 90 min increased the levels of the inflammatory cytokines, monocyte chemotactic protein-1 (MCP-1), and interleukin-1β (IL-1β) in skeletal muscles, especially in vastus lateralis, and the plasma creatine kinase levels. These increases were attenuated by a single oral administration of orange peel extract (OPE) 30 min before downhill running. A mixture of nobiletin, sinensetin, 3,5,6,7,8,3′,4′-heptamethoxyflavone, and tangeretin, which are the major PMFs of OPE, also showed similar effects on muscle damage. These results suggest that OPE has a protective effect against eccentric exercise-induced skeletal muscle damage, and that the effects may be attributed to the 4 major PMFs.

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Effect of dietary orange peel extract on physiological, biochemical, and metabolic responses of Japanese quail reared under low ambient temperature

TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES ◽

10.3906/vet-1504-11 ◽

2016 ◽

Vol 40 ◽

pp. 288-297 ◽

Cited By ~ 6

Author(s):

Mehmet ÇİFTÇİ ◽

Ülkü Gülcihan ŞİMŞEK ◽

Bestami DALKILIÇ ◽

Mehmet Ali AZMAN ◽

Ökkeş YILMAZ ◽

...

Keyword(s):

Ambient Temperature ◽

Japanese Quail ◽

Orange Peel ◽

Metabolic Responses ◽

Orange Peel Extract ◽

Peel Extract ◽

Low Ambient Temperature

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Efficient and scalable method in isolation of polymethoxyflavones from orange peel extract by supercritical fluid chromatography

Journal of Chromatography B ◽

10.1016/j.jchromb.2006.09.010 ◽

2007 ◽

Vol 846 (1-2) ◽

pp. 291-297 ◽

Cited By ~ 46

Author(s):

Shiming Li ◽

Ted Lambros ◽

Zhenyu Wang ◽

Robert Goodnow ◽

Chi-Tang Ho

Keyword(s):

Supercritical Fluid ◽

Supercritical Fluid Chromatography ◽

Orange Peel ◽

Orange Peel Extract ◽

Peel Extract

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Effect of Encapsulated Bitter Orange Peel Extract and BHT on the Quality of Common Carp Fillet during Refrigerated Storage

International Journal of Food Engineering ◽

10.1515/ijfe-2015-0185 ◽

2016 ◽

Vol 12 (3) ◽

pp. 303-310 ◽

Cited By ~ 2

Author(s):

Omid Hasani ◽

Seyed Rohollah Javadian

Keyword(s):

Lipid Oxidation ◽

Common Carp ◽

Storage Period ◽

Orange Peel ◽

Thiobarbituric Acid ◽

Refrigerated Storage ◽

High Concentration ◽

Bitter Orange ◽

Orange Peel Extract ◽

Peel Extract

Abstract The aim of the present study was to evaluate antioxidative activities of the encapsulated and unencapsulated bitter orange peel extract on the shelf-life of the common carp fillets during 16 days of refrigerated storage. Common carp fillets were treated with bitter orange peel extract (0.5 and 1% w/w), liposomal encapsulated bitter orange peel extract (0.5 and 1% w/w) and beta-hydroxytoluene (BHT, 100 and 200 ppm); their quality changes in terms of total volatile basic nitrogen (TVB-N), free fatty acid (FFA) peroxide value (PV), thiobarbituric acid (TBA) and sensory characteristics were investigated. Results showed that the encapsulated and pure extract and BHT could reduce chemical deterioration and lipid oxidation in the fillets compared to the control, as reflected with lower TVBN, FFA, PV and TBA values. Furthermore, common carp fillets treated with high concentration (1%) of the encapsulated bitter orange peel extract showed the lowest amount of lipid oxidation during the storage period compared with the others without any undesirable changes in sensorial attributes.

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Cachectin/TNF or IL-1 alpha induces cachexia with redistribution of body proteins

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1989.256.3.r659 ◽

1989 ◽

Vol 256 (3) ◽

pp. R659-R665 ◽

Cited By ~ 25

Author(s):

Y. Fong ◽

L. L. Moldawer ◽

M. Marano ◽

H. Wei ◽

A. Barber ◽

...

Keyword(s):

Skeletal Muscle ◽

Protein Content ◽

Muscle Protein ◽

Interleukin 1 ◽

Metabolic Effects ◽

Liver Protein ◽

Mrna Levels ◽

Skeletal Muscle Protein ◽

Male Wistar Rats

Macrophage secretory products are suspected to participate in the severe lean tissue wasting related to chronic illness. The protein metabolic effects of chronic, 7-day cachectin/tumor necrosis factor (cachectin) or interleukin 1 alpha (IL-1 alpha) administration in vivo were studied in male Wistar rats that were 1) freely fed, 2) pair fed, 3) total protein and calorie starved, 4) twice daily lipopolysaccharide (LPS) administered, 5) twice daily cachectin administered, and 6) twice daily IL-1 alpha administered. LPS, cachectin, or IL-1 alpha administration produced anorexia; weight loss in these groups was comparable to respective pair-fed animals. However, LPS, cachectin, or IL-1 alpha accelerated peripheral protein wasting while preserving liver protein content, unlike the pattern in the pair-fed or starved animals in which loss of liver proteins and relative preservation of skeletal muscle protein were observed. The decrease in skeletal muscle protein content in LPS- or cytokine-treated animals was associated with coordinate decreases in muscle mRNA levels for the myofibrillar proteins myosin heavy chain, myosin light chain, actin, and in the 18S and 28S subunits of ribosomal RNA. We conclude that chronic exposure to the cytokines, IL-1 alpha or cachectin, can simulate those body and muscle protein changes seen in experimental LPS administration or chronic disease and markedly differ from the pattern of protein redistribution due to caloric restriction.

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