Proteinous pancreatic lipase inhibitor is responsible for the antiobesity effect of young barley (Hordeum vulgare L.) leaf extract

2021 ◽  
Author(s):  
Eisuke Kato ◽  
Ai Tsuruma ◽  
Ayaka Amishima ◽  
Hiroshi Satoh
Keyword(s):  
Hordeum Vulgare ◽  
Bioactive Compounds ◽  
Pancreatic Lipase ◽  
Leaf Extract ◽  
Health Effect ◽  
Fructose Bisphosphate ◽  
Hordeum Vulgare L ◽  
Lipase Inhibitor ◽  
Barley Leaves ◽  
Health Promoting

Abstract Young barley leaves (Hordeum vulgare L.) have various health effects and are employed as an ingredient in the production of health-promoting foods. Promoting antiobesity is one such health effect; however, the mechanism and bioactive compounds are unclear. In this research, young barley leaf extract (YB) was demonstrated to possess pancreatic lipase inhibitory activity. The addition of YB to a high-fat diet in mice increased fecal lipid content, indicating reduced absorption of lipids as the mechanism underlying antiobesity effect. The investigation of bioactive compounds in YB resulted in the identification of fructose–bisphosphate aldolase as a proteinous lipase inhibitor. Maximum inhibition of the protein was 45%, but inhibition was displayed at a concentration as low as 16 ng/mL, which is a characteristic inhibition compared with other reported proteinous lipase inhibitors.

scholarly journals Two isoforms of a nucleotide-sugar pyrophosphatase/phosphodiesterase from barley leaves (Hordeum vulgare L.) are distinct oligomers of HvGLP1, a germin-like protein

FEBS Letters ◽  
2001 ◽  
Vol 490 (1-2) ◽  
pp. 44-48 ◽  
Author(s):  
Milagros Rodrı́guez-López ◽  
Edurne Baroja-Fernández ◽  
Aitor Zandueta-Criado ◽  
Beatriz Moreno-Bruna ◽  
Francisco José Muñoz ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Hordeum Vulgare L ◽  
Nucleotide Sugar ◽  
Barley Leaves

scholarly journals Variability of Hordeum vulgare L. Cultivars in Yield, Antioxidant Potential, and Cholinesterase Inhibitory Activity

2020 ◽  
Vol 12 (5) ◽  
pp. 1938 ◽  
Author(s):  
Joanna Kobus-Cisowska ◽  
Piotr Szulc ◽  
Oskar Szczepaniak ◽  
Marcin Dziedziński ◽  
Daria Szymanowska ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Inhibitory Activity ◽  
Inhibitory Effect ◽  
Antioxidant Potential ◽  
Aqueous Extracts ◽  
Hordeum Vulgare L ◽  
Barley Cultivars ◽  
Cholinesterase Inhibitory Activity ◽  
Barley Leaves ◽  
Barley Grains

Barley (Hordeum vulgare L.) is an annual plant cultivated in spring or autumn. Currently, over 70% of the cultivated barley grains are utilized for preparing fodder, while the rest is used for the production of malt and cereals in the food industry. The purpose of the present work was to evaluate the content of bioactive compounds, antioxidant potential, and cholinesterase inhibitory effect of the aqueous extracts of juvenile barley leaves. It was found that the barley cultivars differed in their content of the determined phytochemicals as well as their antioxidant potential and cholinesterase-inhibitory activity. The water extracts of young barley leaves contained phenolic acids as well as quercetin, rutin, and kaempferitrin. The extracts showed a higher inhibitory effect on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) than on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. Based on the aqueous extracts analyzed, we found that winter cultivars were characterized by the highest iron-chelating activity. Furthermore, barley extracts showed a stronger inhibitory effect against acetylcholinesterase compared to butyrylcholinesterase. The results of the present work indicated that barley cultivars differed in their germination process. Among the tested samples, the highest cholinesterase inhibitory activity was shown by the Basic variety.

scholarly journals Effects of multiple abiotic stresses on lipids and sterols profile in barley leaves (Hordeum vulgare L.)

2019 ◽  
Vol 141 ◽  
pp. 215-224 ◽  
Author(s):  
Anetta Kuczyńska ◽  
Vladimiro Cardenia ◽  
Piotr Ogrodowicz ◽  
Michał Kempa ◽  
Maria Teresa Rodriguez-Estrada ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Abiotic Stresses ◽  
Hordeum Vulgare L ◽  
Barley Leaves

Metabolism of glycolic acid-1-14C in barley leaves with or without added CO2

1971 ◽  
Vol 49 (2) ◽  
pp. 299-302 ◽  
Author(s):  
Imre A. Tamàs ◽  
R. G. S. Bidwell
Keyword(s):  
Hordeum Vulgare ◽  
Glycolic Acid ◽  
Calvin Cycle ◽  
Sucrose Synthesis ◽  
Hordeum Vulgare L ◽  
Barley Leaves

Detached primary leaves of barley (Hordeum vulgare L. var. Dayton) were supplied with glycolate-1 -14C in light or darkness with or without CO2, and the radioactivity of soluble metabolites and evolved CO2 was measured at intervals. The results of these experiments suggest that exogenously supplied glycolate is metabolized by two distinct pathways in illuminated leaves. It may be converted to sucrose outside the chloroplasts, presumably via the glycolate pathway. Alternatively glycolate carbon, through some product of its metabolism, enters the chloroplasts and becomes incorporated into intermediates of the Calvin cycle, from which photorespired CO2 is derived. Endogenous glycolate, originating from Calvin cycle intermediates, may leave the chloroplasts and become a substrate for sucrose synthesis. Exchange of carbon therefore appears to take place between the Calvin cycle inside and the glycolate pathway outside the chloroplasts. This exchange gives a net flow of carbon out of the chloroplasts during photosynthetic CO2 fixation. However, at low external CO2 concentration the flow of carbon from the glycolate pathway into the Calvin cycle in chloroplasts is greatly enhanced, providing substrate for CO2 production. Exogenous glycolate apparently does not directly enter the site of CO2 production in light. However, it is converted to CO2 in darkness, without the participation of the Calvin cycle.

scholarly journals Acute Oral Toxicity and Genotoxicity of Polysaccharide Fraction from Young Barley Leaves (Hordeum vulgare L.)

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 809
Author(s):  
Chang-Won Cho ◽  
Young-Ran Song ◽  
Won-Chul Lim ◽  
Youn-Hwan Hwang ◽  
Young Kyoung Rhee ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Chromosomal Aberration ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Hordeum Vulgare L ◽  
Polysaccharide Fraction ◽  
Sd Rats ◽  
Barley Leaves ◽  
In Vitro Genotoxicity

Polysaccharides isolated from various plants are considered precious bioactive materials owing to their potent biological activities. Previously, we prepared a polysaccharide fraction (BLE0) isolated from young barley leaves (Hordeum vulgare L.), demonstrating its anti-osteoporotic and immunostimulatory activities. However, data regarding BLE0 toxicity is lacking. To establish its safety, in vitro genotoxicity (chromosomal aberration and bacterial reverse mutation assays) and acute oral toxicity assays were conducted. In the in vitro genotoxicity assays, bacterial reverse mutation and chromosomal aberration assays showed that BLE0 possessed no mutagenicity or clastogenicity. Furthermore, the median lethal dose (LD50) of BLE0 was higher than 5000 mg/kg in female and male Sprague-Dawley (SD) rats and no adverse effects were observed in terms of mortality and abnormal changes in clinical signs (body weight and necropsy). Based on these results, BLE0 was found to be safe with regards to genotoxicity under our test conditions, demonstrating no acute oral toxicity up to 5000 mg/kg in SD rats.

Induction of Hydroxycinnamic Acid Amides and Tryptophan by Jasmonic Acid, Abscisic Acid and Osmotic Stress in Barley Leaves

2001 ◽  
Vol 56 (3-4) ◽  
pp. 193-202 ◽  
Author(s):  
Yuki Ogura ◽  
Atsushi Ishihara ◽  
Hajime Iwamura
Keyword(s):  
Abscisic Acid ◽  
Hordeum Vulgare ◽  
Osmotic Stress ◽  
Jasmonic Acid ◽  
Secondary Metabolism ◽  
Hydroxycinnamic Acid ◽  
Spectroscopic Methods ◽  
Hordeum Vulgare L ◽  
Hydroxycinnamic Acid Amides ◽  
Barley Leaves

AbstractThe effects of jasmonic acid (JA) and abscisic aid (ABA) on secondary metabolism in barley (Hordeum vulgare L.) were investigated. Treatment with JA at 100 μᴍ for 48 h induced accumulation of four compounds in barley primary leaves. The accumulation of these com­pounds was also observed after treatment with ABA at 100 μᴍ. The induced compounds were identified as p-coumaroylputrescine, p-coumaroylagmatine, p-coumaroyl-3-hydroxyagmatine and tryptophan by spectroscopic methods. The profiles of compounds induced by application of JA and ABA were different. JA exhibited stronger inducing activity for hydroxycinnamic acid amides than ABA, while ABA was more active in tryptophan accumulation. The major hydroxycinnamic acid amides in JA-and ABA-treated leaves were p-coumaroylagmatine and p-coumaroyl-3-hydroxyagmatine, respectively. These differences suggested that JA and ABA act in distinct modes. The induction of these compounds was also observed in leaf segments treated with 1 ᴍ sorbitol and glucose. These findings suggested that JA and ABA are involved in accumulation of hydroxycinnamic acid amides and tryptophan in response to osmotic stress in barley.

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