scholarly journals 814 No Animals Were Harmed in The Making of This Microsurgical Model. A Novel Microsurgery Practice Model

2021 ◽  
Vol 108 (Supplement_6) ◽  
Author(s):  
M Abdelaty ◽  
A Molajo
Keyword(s):  
Rat Model ◽  
Infection Risk ◽  
Animal Tissue ◽  
Surgical Glove ◽  
Practice Model ◽  
Warm Up ◽  
Plastic And Reconstructive Surgery ◽  
Training Opportunities ◽  
Anaesthetised Rats ◽  
Novel Model

Abstract Introduction Microsurgery forms the cornerstone of plastic and reconstructive surgery. Traditionally this is taught and practiced using anaesthetised rats or the chicken leg model. Whilst these models described provide useful training opportunities for surgeons, they present their own logistical difficulties. The rat model requires a license to utilise animal tissue. There are potential infection risks of using raw chicken for microsurgery practice We present a novel model to overcome the problems discussed above and allow microsurgery practice. Method A surgical glove is gently stretched over a gallipot. Two parallel longitudinal incisions are made using a scalpel. The warmup stage involves tubularising the section of surgical glove. The created tube is then divided, and anastomosis is performed. Multiple anastomoses can be performed along the length of the tube. Results The model has been used for warm up and anastomosis practice. It removes the barriers that make regular microsurgery practice difficult - namely access to anaesthetised animals (licensed facility required) and raw chicken (messy and potential infection risk) Conclusions We introduce a novel and accessible microsurgical practice model. It is used for learning and maintaining microsurgical skills and circumvents the barriers of previously described models.

scholarly journals A novel rat model of foreign body osteomyelitis for evaluation of antimicrobial efficacy

2019 ◽  
Vol 3 (1) ◽  
pp. 7-14
Author(s):  
Cassandra L. Brinkman ◽  
Suzannah M. Schmidt-Malan ◽  
Melissa J. Karau ◽  
Robin Patel
Keyword(s):  
Foreign Body ◽  
Animal Models ◽  
Rat Model ◽  
Staphylococcus Epidermidis ◽  
Foreign Bodies ◽  
Infected Animal ◽  
Infection Site ◽  
Aureus Infection ◽  
Novel Model ◽  
A Current

The most common organism-type causing orthopedic foreign body infection is the staphylococci, of which Staphylococcus aureus and Staphylococcus epidermidis are especially common. These organisms form biofilms on orthopedic foreign body surfaces, rendering such infections challenging and time consuming to treat. Our group evaluates novel therapeutics for orthopedic foreign body infection in animal models. A current limitation of most animal models is that that they only allow for the removal of one sample per animal, at the time of sacrifice. Herein, we describe a novel rat model of foreign body osteomyelitis that allows removal of foreign bodies at different time points, from the same infected animal. We demonstrate that this model can be used for both S. aureus and S. epidermidis orthopedic foreign body infection, with 3.56, 3.60 and 5.51 log10 cfu/cm2 S. aureus recovered at four, five and six weeks, respectively, after infection, and 2.08, 2.17 and 2.62 log10 cfu/cm2 S. epidermidis recovered at four, five and six weeks, respectively, after infection. We evaluated the model with S. aureus infection treated with rifampin 25 mg/kg twice daily for 21 days. Using quantitative cultures, we were no longer able to detect bacteria as of the 14th day of treatment with bacteria becoming detectable again 7 days following the discontinuation of rifampin a period. This novel model allows monitoring of evolution of infection at the infection site in the same animal.

scholarly journals A Novel Model of Mixed Vascular Dementia Incorporating Hypertension in a Rat Model of Alzheimer’s Disease

2019 ◽  
Vol 10 ◽  
Author(s):  
Paul Denver ◽  
Heather D’Adamo ◽  
Shuxin Hu ◽  
Xiaohong Zuo ◽  
Cansheng Zhu ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Vascular Dementia ◽  
Rat Model ◽  
Model Of Alzheimer’S Disease ◽  
Novel Model

Application of ruthenium red/osmium tetroxide to bacteria, plant and animal tissue

1986 ◽  
Vol 44 ◽  
pp. 54-55
Author(s):  
R. L. Grayson ◽  
N. A. Rechcigl
Keyword(s):  
Electron Microscopy ◽  
Electron Density ◽  
Osmium Tetroxide ◽  
Biological Materials ◽  
Ruthenium Red ◽  
Animal Tissue

Ruthenium red (RR), an inorganic dye was found to be useful in electron microscopy where it can combine with osmium tetroxide (OsO4) to form a complex with attraction toward anionic substances. Although Martinez-Palomo et al. (1969) were one of the first investigators to use RR together with OsO4, our computor search has shown few applications of this combination in the intervening years. The purpose of this paper is to report the results of our investigations utilizing the RR/OsO4 combination to add electron density to various biological materials. The possible mechanisms by which this may come about has been well reviewed by previous investigators (1,3a,3b,4).

Immunoelectron microscopic localization of elastic tissue in archival material using an improved method

1990 ◽  
Vol 48 (3) ◽  
pp. 794-795
Author(s):  
J. C. Fanning ◽  
J. F. White ◽  
R. Polewski ◽  
E. G. Cleary
Keyword(s):  
Normal Animal ◽  
Animal Tissue ◽  
Elastic Tissue ◽  
Human Tissues ◽  
Improved Method ◽  
Tissue Samples ◽  
Archival Material ◽  
Immuno Electron Microscopy ◽  
Arteries And Veins ◽  
Biochemical Examination

Elastic tissue is an important component of the walls of arteries and veins, of skin, of the lungs and in lesser amounts, of many other tissues. It is responsible for the rubber-like properties of the arteries and for the normal texture of young skin. It undergoes changes in a number of important diseases such as atherosclerosis and emphysema and on exposure of skin to sunlight.We have recently described methods for the localizationof elastic tissue components in normal animal and human tissues. In the study of developing and diseased tissues it is often not possible to obtain samples which have been optimally prepared for immuno-electron microscopy. Sometimes there is also a need to examine retrospectively samples collected some years previously. We have therefore developed modifications to our published methods to allow examination of human and animal tissue samples obtained at surgery or during post mortem which have subsequently been: 1. stored frozen at -35° or -70°C for biochemical examination; 2.

Telepractice Training for Early Intervention with Children who are Deaf/Hard-of-Hearing

2016 ◽  
Vol 1 (9) ◽  
pp. 60-67
Author(s):  
Kristina M. Blaiser ◽  
Diane Behl
Keyword(s):  
Early Intervention ◽  
Hard Of Hearing ◽  
Communication Disorders ◽  
Staff Support ◽  
Early Intervention Services ◽  
Part C ◽  
Training Courses ◽  
Part C Early Intervention ◽  
Training Opportunities

Telepractice is an increasingly popular service delivery model for serving individuals with communication disorders, particularly infants and toddlers who are Deaf/Hard-of-Hearing (DHH) served under Part C Early Intervention programs (Behl, Houston, & Stredler-Brown, 2012). Recent studies have demonstrated that telepractice is effective for providing children who are DHH and their families with access to high quality early intervention services (Behl et al., 2016; Blaiser, Behl, Callow-Heusser, & White, 2013). While telepractice has grown in popularity, there continues to be a lack of formalized training opportunities to help providers become more familiar with telepractice (Behl & Kahn, 2015). This paper outlines online training courses for providers, families, and administrators of programs for children who are DHH. Recommendations for follow up training and staff support are included.

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