scholarly journals Longan Pulp Polysaccharide Protects Systemic Immunity and Intestinal Immunity in Mice Induced by Cyclophosphamide

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 738-738
Author(s):  
Yajuan Bai ◽  
Mingwei Zhang
Keyword(s):  
Mucosal Immunity ◽  
Plasma Cells ◽  
Underlying Mechanism ◽  
Secretory Component ◽  
Secretory Iga ◽  
Intestinal Lumen ◽  
Mucosal Barrier ◽  
Intestinal Immunity ◽  
Systemic Immunity ◽  
Serum Iga

Abstract Objectives This study aimed to explore the effect of longan pulp polysaccharide (LP) on the systemic immunity and intestinal mucosal immunity with immunosuppressive mice. The synthesis processing and secretion of intestinal secretory IgA (SIgA) were investigated. Methods Serum IgA, IgG, IgM and intestinal SIgA were detected by ELISA. Genes involved in the synthesis and secretion of SIgA were detected by Q-PCR and western blot. Results LP increased the thymus index, spleen index, and serum IgA level in cyclophosphamide (CTX)-treated mice. SIgA secretion in intestinal lumen was increased by LP as well. The underlying mechanism comes down to the facts as follows: LP increased intestinal cytokines expression and TGFβRII that is associated with pathways of IgA class switch recombination (CSR). By improving protein expression of mucosal address in cell-adhesion molecule-1 (MAdCAM-1) and integrin α4β7, LP was beneficial to gut homing of IgA + plasma cells. LP increased IgA, polymeric immunoglobulin receptor (pIgR), and secretory component (SC) to fortify the SIgA secretion. Conclusions This study suggested that moderate consumption of LP is helpful for improving systemic immunity and intestinal mucosal immunity via promotion of intestinal SIgA to strengthen the mucosal barrier. Funding Sources This work was supported by the National Key Research Project of China (2018YFC1602105, 2019YFD1002304), Guangdong Provincial Science and Technology Project (2018A050506050), President Foundation of Guangdong Academy of Agricultural Sciences (201812B).

Secretory Immunoglobulin a in Oral and Respiratory Passages in Man

1975 ◽  
Vol 84 (20_suppl) ◽  
pp. 1-23 ◽  
Author(s):  
Goro Mogi
Keyword(s):  
Immunoglobulin A ◽  
Secretory Component ◽  
Secretory Iga ◽  
Secretory Cells ◽  
Antigenic Relationship ◽  
Serum Iga ◽  
Human Colostrum ◽  
Specific Antisera ◽  
Antigenic Relationships ◽  
Secretory Immunoglobulin

Secretory IgA (SIgA) is the predominant immunoglobulin in certain external secretions and may have an important role in immunological mucosal resistance. SIgA differs in chemical and immunological properties from serum IgA. The present study was undertaken to investigate the antigenic relationship between SIgA, free secretory component (FSC) and serum IgA and the localization of SIgA as well as other immunological classes in tissues of oral and respiratory passages by use of immunofluorescence technique. SIgA and FSC were highly purified from human colostrum and rabbit anti-SIgA and anti-SC antisera were prepared. On the basis of antigenic relationships between SIgA, FSC and serum IgA, it was emphasized that individual specific antisera for SC and IgA and/or SIgA should be used in immunochemical or immunohistological investigations for SIgA. The present study failed to detect SC determinants in palatine and lingual tonsils. However, it was evident that cells present in the pharyngeal tonsillar epithelium contain SC determinants. SC molecules may be synthesized in certain secretory cells of mucous membrane and glandular epithelium and the combining of SC with IgA could occur in the cytoplasm of epithelial cells, the intercellular spaces and/or in the lumens of glandular acini and ductules.

scholarly journals Effect of Peptides from Alaska Pollock on Intestinal Mucosal Immunity Function and Purification of Active Fragments

Nutrients ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 2517
Author(s):  
Qiqi Li ◽  
Shikai Wang ◽  
Supanooch Poungchawanwong ◽  
Hu Hou
Keyword(s):  
Body Weight ◽  
Mucosal Immunity ◽  
Plasma Cells ◽  
Intestinal Barrier ◽  
Mucosal Barrier ◽  
Immune Functions ◽  
Immune Organ ◽  
Intestinal Mucosal Barrier ◽  
Active Fragments ◽  
Immunity Function

The intestinal mucosal barrier plays an important role in systemic immune functions. This study aimed to find the mechanism of peptide from Alaska Pollock (APP) on intestinal mucosal immunity in mice induced by cyclophosphamide (Cy). Cy-induced decreases of body weight and index of immune organ were significantly improved by APP as compared with Cy group (p < 0.05). APP could promote the secretion of SIgA and IgA on intestinal mucosa (p < 0.05) and mainly had an impact on the final differentiation of IgA+ B cell, thereby promoting the secretion of plasma cells, which can be corroborated by the increases of IL-6 and IL-10 (p < 0.05). APP with high immune activity was separated and two peptides were purified and identified as Gly–Val–Ile–Lys and Ala–Cys–Asn–Gly–Arg. Therefore, APP can be considered as beneficial ingredients to protect the intestinal barrier disruption induced by Cy.

scholarly journals Coated zinc oxide improves intestinal immunity function and regulates microbiota composition in weaned piglets

2014 ◽  
Vol 111 (12) ◽  
pp. 2123-2134 ◽  
Author(s):  
Junhua Shen ◽  
Yan Chen ◽  
Zhisheng Wang ◽  
Anguo Zhou ◽  
Miao He ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Diversity Index ◽  
Secretory Iga ◽  
Mucosal Barrier ◽  
Jejunal Mucosa ◽  
Microbiota Composition ◽  
Intestinal Immunity ◽  
Weaned Piglets ◽  
Immunity Function

The present study was conducted to test the hypothesis that low concentrations of coated ZnO, as a substitute for a high concentration of ZnO (2250 mg Zn/kg), could improve intestinal immunity function and regulate microbiota composition, thus alleviating the incidence of diarrhoea in weaned piglets. A total of eighty-four cross-bred piglets, weaned at an age of 28 (sem 1) d, were allocated randomly, on the basis of average initial body weight (7·72 (sem 0·65) kg), to seven treatment groups as follows: a 250 mg Zn (ZnO)/kg group (low Zn; LZ) and a 2250 mg Zn (ZnO)/kg group (high Zn; HZ) that were offered diets containing ZnO at 250 and 2250 mg Zn/kg, respectively; and five experimental groups in which coated ZnO was added at 250, 380, 570, 760 and 1140 mg Zn/kg basal diet, respectively. The trial lasted 2 weeks. The results indicated that, compared with LZ treatment, supplementation with coated ZnO at 380 or 570 mg Zn/kg reduced (P< 0·05) diarrhoea index, increased (P< 0·05) duodenal villus height and the ratio of villus height:crypt depth, up-regulated (P< 0·05) the gene expression of insulin-like growth factor 1, zonula occludens protein-1, occludin, IL-10 and transforming growth factor β1, and elevated (P< 0·05) secretory IgA concentration in the jejunal mucosa. Microbiota richness and the Shannon diversity index were also decreased (P< 0·05). Furthermore, piglets in the group fed coated ZnO at 380 or 570 mg Zn/kg did not differ from those in the HZ-fed group in relation to the aforementioned parameters. Collectively, a low concentration of coated ZnO (380 or 570 mg Zn/kg) can alleviate the incidence of diarrhoea by promoting intestinal development, protecting the intestinal mucosal barrier from damage, stimulating the mucosal immune system and regulating the microbiota composition.

scholarly journals REGULATION OF THE IMMUNE RESPONSE: SUPPRESSIVE AND ENHANCING EFFECTS OF PASSIVELY ADMINISTERED ANTIBODY

1971 ◽  
Vol 133 (5) ◽  
pp. 987-1003 ◽  
Author(s):  
Carolyn S. Pincus ◽  
Michael E. Lamm ◽  
Victor Nussenzweig
Keyword(s):  
Immune Response ◽  
Antibody Formation ◽  
Secretory Component ◽  
Secretory Iga ◽  
Antigenic Determinants ◽  
Antibody Synthesis ◽  
Serum Iga ◽  
Iga Antibody ◽  
Antigen Antibody

The ability of passively administered antibody to suppress the immune response against homologous antigenic determinants while concomitantly enhancing the response against other unrelated determinants of the same antigen molecule has been established in two distinct antigen-antibody systems: (a) guinea pig γ2-immunoglobulin + passive anti-F(ab')2 antibody, where suppression of anti-F(ab')2 antibody synthesis is accompanied by enhancement of the anti-Fc response; and (b) human secretory IgA + passive anti-serum IgA antibody, where suppression of antibody production against the α and L chains accompanies augmentation of the response to the secretory component. The mechanisms of the suppressive and enhancing effects are probably unrelated for the following reasons: (a) Enhancement of the response to certain determinants may be obtained without discernible suppression of the response to the homologous determinants; and (b) the F(ab')2 fragments of passive antibody can mediate immune suppression but were not observed to enhance the response against the unrelated determinants of the same antigen molecule. Also, the timing for achieving maximum suppression or enhancement of antibody formation is not the same; enhancement was obtained only at a later time. Both the enhancement and suppressive effects were obtained with the purified γG fraction of antisera. This finding rules out an exclusive role of γM antibody in the enhancement phenomenon.

scholarly journals High levels of secretory IgA and free secretory component in the serum of rats with bile duct obstruction.

1978 ◽  
Vol 147 (3) ◽  
pp. 934-939 ◽  
Author(s):  
I Lemaître-Coelho ◽  
G D Jackson ◽  
J P Vaerman
Keyword(s):  
Bile Duct ◽  
Rat Liver ◽  
Secretory Component ◽  
Bile Duct Obstruction ◽  
Secretory Iga ◽  
Duct Obstruction ◽  
Serum Iga ◽  
Secretory Type

In the rat , ligation of the bile duct induces a rapid and progressive elevation of the IgA levels in serum. The increase is about 4-fold at 1 h, 15-fold at 1 day, and 30-fold at 1 wk after ligation. The additional IgA is of the secretory type. Free secretory component also appears in serum after bile duct obstruction; it does not continue to increase and occasionally disappears from serum after prolonged ligation. The increase in serum IgA levels is selective. These changes are totally reversible if the bile duct is reopened at 1 day after ligature. These findings confirm the role of the rat liver in the transfer of circulating IgA into the bile.

scholarly journals The Effects of Secretory IgA in the Mucosal Immune System

2020 ◽  
Vol 2020 ◽  
pp. 1-6 ◽  
Author(s):  
Yue Li ◽  
Liang Jin ◽  
Tongxin Chen
Keyword(s):  
Immune System ◽  
Critical Role ◽  
Immunoglobulin A ◽  
Secretory Component ◽  
Mucosal Immune System ◽  
Secretory Iga ◽  
Antibody Isotype ◽  
Serum Iga ◽  
Important Field ◽  
Immune Exclusion

Immunoglobulin A (IgA) is the most abundant antibody isotype in the mucosal immune system. Structurally, IgA in the mucosal surface is a polymeric structure, while serum IgA is monomeric. Secretory IgA (sIgA) is one of the polymeric IgAs composed of dimeric IgA, J chain, and secretory component (SC). Most of sIgAs were generated by gut and have effects in situ. Besides the function of “immune exclusion,” a nonspecific immune role, recent studies found it also played an important role in the specific immunity and immunoregulation. Thanks to the critical role of sIgA during the mucosal immune system homeostasis between commensal microorganisms and pathogens; it has been an important field exploring the relationship between sIgA and commensal microorganisms.

Local Immune System in Human Adult and Fetal Larynx

1981 ◽  
Vol 89 (3) ◽  
pp. 471-476 ◽  
Author(s):  
Tadashi Nakashima ◽  
Kazumi Makishima
Keyword(s):  
Immune System ◽  
Connective Tissue ◽  
Plasma Cells ◽  
Secretory Activity ◽  
Secretory Component ◽  
Glandular Epithelium ◽  
Secretory Iga ◽  
Human Fetuses ◽  
Tissue Area ◽  
Submucosal Glands

Using immunofluorescent and histopathologic techniques, we studied the distribution and secretory activity of the glands of the larynx from 51 adults and 15 human fetuses. Glandular buds were first observed at the 12th fetal week and glandular distribution became evident from the 16th fetal week. In the adult larynx, intraepithelial and submucosal glandular distribution was observed in each specimen. Immunofluorescent studies revealed the diffuse fluorescence for IgA not only in the submucosal or periglandular connective tissue area but also in the intraepithelial and submucosal glands. Secretory component (SC) synthesis was found mainly in each serous-type glandular epithelium or acinus. In the submucosal or periglandular area, IgA-producing plasma cells were numerous. In contrast, there was little fluorescence for IgA in the fetal laryngeal tissues. Secretory component, however, was found in each serous-type glandular epithelium or acinus from the 16th week. Our observations suggest that local immune system by secretory IgA is active in the adult larynx, and the production of SC is inherently acquired in the fetal larynx.

Secretory Immunoglobulin a in Oral and Respiratory Passages in Man

1975 ◽  
Vol 84 (3_suppl) ◽  
pp. 2-23 ◽  
Author(s):  
Goro Mogi
Keyword(s):  
Immunoglobulin A ◽  
Secretory Component ◽  
Secretory Iga ◽  
Secretory Cells ◽  
Antigenic Relationship ◽  
Serum Iga ◽  
Human Colostrum ◽  
Specific Antisera ◽  
Antigenic Relationships ◽  
Secretory Immunoglobulin

Secretory IgA (SIgA) is the predominant immunoglobulin in certain external secretions and may have an important role in immunological mucosal resistance. SIgA differs in chemical and immunological properties from serum IgA. The present study was undertaken to investigate the antigenic relationship between SIgA, free secretory component (FSC) and serum IgA and the localization of SIgA as well as other immunological classes in tissues of oral and respiratory passages by use of immunofluorescence technique. SIgA and FSC were highly purified from human colostrum and rabbit anti-SIgA and anti-SC antisera were prepared. On the basis of antigenic relationships between SIgA, FSC and serum IgA, it was emphasized that individual specific antisera for SC and IgA and/or SIgA should be used in immunochemical or immunohistological investigations for SIgA. The present study failed to detect SC determinants in palatine and lingual tonsils. However, it was evident that cells present in the pharyngeal tonsillar epithelium contain SC determinants. SC molecules may be synthesized in certain secretory cells of mucous membrane and glandular epithelium and the combining of SC with IgA could occur in the cytoplasm of epithelial cells, the intercellular spaces and/or in the lumens of glandular acini and ductules.

scholarly journals Structure of the Human Secretory Immunoglobulin M Core

2020 ◽  
Author(s):  
Nikit Kumar ◽  
Christopher P. Arthur ◽  
Claudio Ciferri ◽  
Marissa L. Matsumoto
Keyword(s):  
Mucosal Immunity ◽  
Secretory Component ◽  
Immunoglobulin M ◽  
Secretory Iga ◽  
Human Antibodies ◽  
Sequence Identity ◽  
J Chain ◽  
Binding Interface ◽  
Mucosal Secretions ◽  
Secretory Immunoglobulin

AbstractImmunoglobulins (Ig) A and M are the only human antibodies that form oligomers and undergo transcytosis to mucosal secretions via the polymeric Ig receptor (pIgR). When complexed with the J-chain (JC) and the secretory component (SC) of pIgR, secretory IgA and IgM (sIgA and sIgM) play critical roles in host-pathogen defense. Recently, we determined the structure of sIgA-Fc which elucidated the mechanism of polymeric IgA assembly and revealed an extensive binding interface between IgA-Fc, JC, and SC. Despite low sequence identity shared with IgA-Fc, IgM-Fc also undergoes JC-mediated assembly and binds pIgR. Here, we report the structure of sIgM-Fc and carryout a systematic comparison to sIgA-Fc. Our structural analysis reveals a remarkably conserved mechanism of JC-templated oligomerization and SC recognition of both IgM and IgA through highly a conserved network of interactions. These studies reveal the structurally conserved features of sIgM and sIgA required for function in mucosal immunity.

LOCAL IMMUNOLOGICAL RESPONSE IN THE VAGINA, CERVIX AND ENDOMETRIUM

1975 ◽  
Vol 80 (2_Suppl) ◽  
pp. S281-S305 ◽  
Author(s):  
J.-P. Vaerman ◽  
J. Férin
Keyword(s):  
Epithelial Cells ◽  
Genital Tract ◽  
Plasma Cells ◽  
Specific Binding ◽  
Female Genital Tract ◽  
Secretory Component ◽  
Secretory Iga ◽  
Local Synthesis ◽  
Female Genital

ABSTRACT The mechanism of the local excretion of secretory IgA (SIgA) in exocrine secretions has been reviewed. Numerous local IgA-plasma cells, in the lamina propria of the glandular mucosa, synthesize dimeric IgA with J-chain. Free secretory component (FSC) is synthesized and accumulated in the Golgi area of the columnar epithelial cells. It is then supposed to get onto their cell membranes. Dimeric IgA (and some IgM) reaches the spaces between and under the epithelial cells. There, a specific binding occurs between dimeric IgA (and some IgM) and the FSC located in the cell-membrane, whereby SIgA is formed. The complex becomes mobilized and is transported toward the apical part of the cell, where it will be excreted into the mucous coat covering the epithelium. In the female genital tract, the cervical mucosa appears to be better adapted to achieve a local secretory immune system. The endometrium seems less suitable, being normally short of local plasma cells. The vaginal wall appears almost incompatible with the proposed mechanism of local antibody secretion. Criteria for establishing a local immune response in the female genital tract comprise: 1) a lack of correlation between antibody titers in secretions and serum; 2) the demonstration that the secretory antibodies are mainly of IgA class and 3) that they are SIgA molecules, possessing bound secretory component. However, the best criterion would be 4) the observation that antibody is actually synthesized in samples of mucosa, by in vitro culture or immunohistology. Reviewing the literature, relatively few examples were found where SIgA antibodies were demonstrated, and unambiguous evidence for their local synthesis is almost non-existent. In addition, the authors were unable to detect antibody-containing cells in cervical and endometrial biopsies of women locally "immunized" with horse spleen ferritin and bovine serum albumin. The need for further investigation with simple antigens and adequate immunological reagents is stressed.

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