Genomic Characterization of Recent and Historic Meningococcal Serogroup E Invasive Disease in Australia: A Case Series

Abstract We report the recent emergence of invasive meningococcal disease due to serogroup E in Queensland, Australia, in previously healthy patients. Molecular typing revealed the genotype of these strains to be E:P1.21-7,16:F5-36:ST-1157 (cc1157); when analyzed phylogenetically, compared with international cc1157 strains, they were relatively unrelated to each other.

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Antimicrobial Resistance and Genomic Characterization of OXA-48- and CTX-M-15-Co-Producing Hypervirulent Klebsiella pneumoniae ST23 Recovered from Nosocomial Outbreak

Antibiotics ◽

10.3390/antibiotics9120862 ◽

2020 ◽

Vol 9 (12) ◽

pp. 862

Author(s):

Elvira Shaidullina ◽

Andrey Shelenkov ◽

Yuri Yanushevich ◽

Yulia Mikhaylova ◽

Dmitriy Shagin ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Illumina Miseq ◽

Type I ◽

Nosocomial Outbreak ◽

Recent Emergence ◽

Genomic Characterization ◽

Global Threat ◽

High Level ◽

Sepsis And Septic Shock

Multidrug resistance (MDR) and hypervirulence (hv) have been long considered distinct evolutionary traits for Klebsiella pneumoniae (Kp), a versatile human pathogen. The recent emergence of Kp strains combining these traits poses a serious global threat. In this article, we describe the phenotypic and genomic characteristics of an MDR hvKp isolate, MAR14-456, representative of a nosocomial outbreak in Moscow, Russia, that was recovered from a postoperative wound in a patient who later developed multiple abscesses, fatal sepsis, and septic shock. Broth microdilution testing revealed decreased susceptibility of MAR14-456 to carbapenems (MICs 0.5–2 mg/L) and a high-level resistance to most β-lactams, β-lactam-β-lactamase-inhibitor combinations, and non-β-lactam antibiotics, except ceftazidime-avibactam, amikacin, tigecycline, and colistin. Whole-genome sequencing using Illumina MiSeq and ONT MinION systems allowed to identify and completely assemble two conjugative resistance plasmids, a typical ‘European’ epidemic IncL/M plasmid that carries the gene of OXA-48 carbapenemase, and an IncFIIK plasmid that carries the gene of CTX-M-15 ESBL and other resistance genes. MLST profile, capsular, lipopolysaccharide, virulence genes encoded on chromosome and IncHI1B/FIB plasmid, and the presence of apparently functional type I-E* CRISPR-Cas system were all characteristic of hvKp ST23, serotype K1-O1v2. Phylogenetic analysis showed the closest relatedness of MAR14-456 to ST23 isolates from China. This report highlights the threat of multiple resistance acquisition by hvKp strain and its spread as a nosocomial pathogen.

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Characterization of Serogroup ANeisseria meningitidisfrom Invasive Meningococcal Disease Cases in Canada Between 1979 and 2006: Epidemiological Links to Returning Travellers

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2008/523021 ◽

2008 ◽

Vol 19 (3) ◽

pp. 227-232 ◽

Cited By ~ 2

Author(s):

Angela M Sloan ◽

Averil M Henderson ◽

Raymond SW Tsang

Keyword(s):

Meningococcal Disease ◽

Clonal Complex ◽

Disease Incidence ◽

Developing Nations ◽

Invasive Meningococcal Disease ◽

Protein Antigens ◽

Genes Encoding ◽

Returning Travellers ◽

The 1960S

INTRODUCTION: Serogroup ANeisseria meningitidishas repeatedly caused epidemics of invasive meningococcal disease (IMD) in developing nations since the 1960s. The present study is the first detailed study of serogroup A bacteria isolated in Canada.METHODS: Thirty-four serogroup A meningococcal isolates collected from individuals with IMD in Canada between 1979 and 2006 were characterized by serology and multilocus sequence typing of seven housekeeping enzyme genes and genes encoding three outer membrane protein antigens.RESULTS: Isolates were assigned to either the sequence type (ST)-1 or the ST-5 clonal complex. Clones within the ST-1 complex were recovered between 1979 and 1992, while clones of the ST-5 complex were isolated between 1987 and 2006; respectively, they accounted for 70.6% and 29.4% of all isolates studied. Isolates of the ST-1 complex were characterized by serosubtype antigen P1.3 or P1.3,6 with PorB allele 60 (serotype 4) and FetA sequence F5-1, while isolates of the ST-5 complex were characterized by serosubtype antigen P1.9 with PorB allele 47 (also serotype 4) and FetA sequence F3-1.CONCLUSIONS: The Canadian serogroup A IMD isolates likely originated in travellers returning from hyperendemic or epidemic areas of the globe where serogroup A bacteria circulate. Although the Canadian cases of serogroup A IMD were caused by clones known to have caused epidemics in developing countries, disease incidence remained low in Canada.

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Secular trends in invasive meningococcal disease, Massachusetts, 1988–2011: what happened to invasive disease?

Epidemiology and Infection ◽

10.1017/s0950268814000259 ◽

2014 ◽

Vol 142 (12) ◽

pp. 2483-2490 ◽

Cited By ~ 1

Author(s):

A. H. PERUSKI ◽

P. KLUDT ◽

R. S. PATEL ◽

A. DeMARIA

Keyword(s):

Meningococcal Disease ◽

Age Groups ◽

Substantial Reduction ◽

Invasive Disease ◽

Secular Trends ◽

Invasive Meningococcal Disease ◽

Age Group ◽

Meningococcal Vaccine ◽

Average Annual Incidence ◽

Potential Factors

SUMMARYInvasive meningococcal disease (IMD) reported to the Massachusetts Department of Public Health from 1988 to 2011 was reviewed. The average annual incidence of IMD/100 000 decreased from 1·57 [95% confidence interval (CI) 1·42–1·73] for 1988–1991 to 0·22 (95% CI 0·17–0·29) for 2008–2011. The pattern of decreasing incidence over time differed by age group. There was a decrease in IMD/100 000 in the 0–4 years age group after 1991 from 10·92 (95% CI 8·08–14·70) in 1991 to 5·76 (95% CI 3·78–8·72) in 1992. Incidence in the 0–4 years age group remained below 5/100 000 per year on average thereafter. A substantial reduction in incidence in all age groups was observed between 2000 and 2009, which began before the introduction of conjugate meningococcal vaccine in 2005. Marked reductions in incidence of IMD in Massachusetts, and elsewhere, deserve further investigation with respect to potential factors that go beyond the introduction and deployment of improved meningococcal vaccines.

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Potential Capsule Switching from Serogroup Y to B: The Characterization of Three suchNeisseria meningitidisIsolates Causing Invasive Meningococcal Disease in Canada

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2005/216369 ◽

2005 ◽

Vol 16 (3) ◽

pp. 171-174 ◽

Cited By ~ 15

Author(s):

Raymond SW Tsang ◽

Dennis KS Law ◽

Shaun D Tyler ◽

Gwen S Stephens ◽

Mark Bigham ◽

...

Keyword(s):

Neisseria Meningitidis ◽

Meningococcal Disease ◽

Multilocus Sequence Typing ◽

Housekeeping Gene ◽

Sequence Type ◽

Invasive Meningococcal Disease ◽

Group B

Three group BNeisseria meningitidisisolates, recovered from meningococcal disease cases in Canada and typed as B:2c:P1.5, were characterized. Multilocus sequence typing showed that all three isolates were related because of an identical sequence type (ST) 573. Isolates typed as 2c:P1.5 are common in serogroup Y meningococci but rare in isolates from serogroups B or C. Although no serogroup Y isolates have been typed as ST-573, eight isolates showed five to six housekeeping gene alleles that were identical to that of ST-573. This suggested that the B:2c:P1.5 isolates may have originated from serogroup Y organisms, possibly by capsule switching.

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Surveillance of invasive meningococcal disease in the Czech Republic

Eurosurveillance ◽

10.2807/esm.09.11.00488-en ◽

2004 ◽

Vol 9 (11) ◽

pp. 11-12 ◽

Cited By ~ 5

Author(s):

P Kriz

Keyword(s):

Czech Republic ◽

Neisseria Meningitidis ◽

Meningococcal Disease ◽

Western Europe ◽

Vaccination Strategy ◽

Invasive Disease ◽

Invasive Meningococcal Disease ◽

Mortality Data ◽

Meningococcal Infections ◽

The Czech Republic

Routine notification of invasive meningococcal disease has a long tradition in the Czech Republic: mortality data are available from 1921 and morbidity data from 1943. The collection of Neisseria meningitidis strains kept in the NRL for Meningococcal Infections in Prague dates from 1970 onwards, and represents more than 3500 strains isolated from invasive disease and their contacts, from healthy carriers and from respiratory infection. Analysis of these strains showed that the Czech meningococcal population is different from that seen in western Europe. In 1993, the incidence serogroup C meningococcal disease increased and was associated with the emergence of the hypervirulent complex Neisseria meningitidis C, ST-11, ET-15/37, and caused an increase in the incidence of invasive meningococcal disease which peaked in 1995 (2.2/100 000). A vaccination strategy targeting the part of the population at highest risk of invasive meningococcal disease was adopted in the country.

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Invasive Meningococcal Disease due to Nongroupable Neisseria meningitidis—Active Bacterial Core Surveillance Sites, 2011–2016

Open Forum Infectious Diseases ◽

10.1093/ofid/ofz190 ◽

2019 ◽

Vol 6 (5) ◽

Cited By ~ 2

Author(s):

Lucy A McNamara ◽

Caelin C Potts ◽

Amy Blain ◽

Nadav Topaz ◽

Mirasol Apostol ◽

...

Keyword(s):

Neisseria Meningitidis ◽

Meningococcal Disease ◽

Invasive Disease ◽

Invasive Meningococcal Disease ◽

Complement Deficiency

Abstract We characterized 22 meningococcal disease cases due to nongroupable Neisseria meningitidis, a rare cause of invasive disease. Disease presentation and severity were similar to those for serogroupable meningococcal disease. However, 7 (32%) patients had complement deficiency or abnormal complement testing results, highlighting the importance of complement testing for nongroupable cases.

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Epidemiological and Molecular Characterization of Invasive Meningococcal Disease in Italy, 2008/09-2012/13

PLoS ONE ◽

10.1371/journal.pone.0139376 ◽

2015 ◽

Vol 10 (10) ◽

pp. e0139376 ◽

Cited By ~ 5

Author(s):

Arianna Neri ◽

Patrizio Pezzotti ◽

Cecilia Fazio ◽

Paola Vacca ◽

Fortunato Paolo D’Ancona ◽

...

Keyword(s):

Molecular Characterization ◽

Meningococcal Disease ◽

Invasive Meningococcal Disease

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Antigenic and genetic characterization of serogroup C meningococci isolated from invasive meningococcal disease cases in Canada from 1999 to 2003

Canadian Journal of Microbiology ◽

10.1139/w05-085 ◽

2005 ◽

Vol 51 (7) ◽

pp. 523-530 ◽

Cited By ~ 8

Author(s):

Dennis K.S Law ◽

Jan Stoltz ◽

Averil M Henderson ◽

Raymond S.W Tsang

Keyword(s):

Membrane Protein ◽

Key Words ◽

Outer Membrane Protein ◽

Meningococcal Disease ◽

Clonal Complex ◽

Genetic Characterization ◽

Hot Spot ◽

Invasive Meningococcal Disease ◽

Vaccination Campaigns

Four hundred and forty-two serogroup C Neisseria meningitidis isolates from individual invasive meningococcal disease (IMD) patients in Canada during the period 1999 to 2003 were analyzed. The majority (84%) of the serogroup C meningococci were characterized by the serotype antigen 2a and belonged to the clonal complex of electrophoretic type ET-15. However, after more than a decade of endemic disease as well as a number of outbreaks and many vaccination campaigns, both genetic and antigenic variants of the serogroup C serotype 2a meningococci were noted. Such variants include strains characterized as C:2a:P1.5 and C:2a:P1.7,1 as well as a non-serotypeable phenotype due to a mutational hot spot on the serotype 2a PorB outer-membrane protein. Meningococci characterized by the antigen formula B:2a:P1.5,2 and B:2a:P1.7,1 have also been found, which suggests capsule switching. Besides the clonal group of ET-15/ET-37, small numbers of serogroup C isolates were found to belong to the clonal complexes of ST-8 (Cluster A4), ST-41/44 (Lineage 3), ST-35, and ST-269.Key words: serogroup C, meningococci, genetic, antigenic, variants.

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