A Gas Chromatographic Method for the Determination of Lactic Acid in Blood

Abstract A method for the determination of lactic acid concentration in blood by gas chroma tography is described. Lactic acid in a protein-free filtrate is oxidized by ceric sulfate to acetaldehyde. The latter compound is introduced into the gas chroma tographic column by vapor phase injection, and is quantitatively measured within 3 min. The method is specific, precise, and not affected by compounds that may interfere with the determination of lactic acid by colorimetric or enzymatic methods. A method for preserving blood samples up to 24 hr. without change in lactic acid concentration is also described.

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Instability of Propyl Lactate Prepared by Boron Trifluoride-Catalyzed Esterification of Lactic Acid

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/55.4.888 ◽

1972 ◽

Vol 55 (4) ◽

pp. 888-889

Author(s):

Walter F Staruszkiewicz ◽

John F Bond

Keyword(s):

Lactic Acid ◽

Ethyl Ether ◽

Boron Trifluoride ◽

Chromatographic Method ◽

Published Data ◽

Chromatographic System ◽

Propyl Esters ◽

Gas Chromatographic Method ◽

Hydrolysis Of

The official first action gas chromatographic method for the determination of lactic, β-hydroxyhutyric, and succinic acids, 17.034–17.038, requires the formation of their propyl esters with boron trifluoride-1-propanol and extraction of the esters into an organic solvent prior to chromatography. Previously published data showed that the solutions of propyl esters were stable for as long as 4 weeks when they were extracted into chloroform and stored at 40°F. However, when ethyl ether is substituted for chloroform, propyl lactate is no longer stable during storage. The ether apparently extracts some unreacted boron trifluoride which eventually causes the hydrolysis of propyl lactate. The propyl esters of β-hydroxybutyric, levulinic, and succinic acids were stable in ether as well as in chloroform. The method has been revised to include the use of ethyl ether, if required by the gas chromatographic system, and to specify analysis of the ether solutions within 24 hr of esterification.

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Gas-chromatographic method for the determination of volatile fatty acids (C1–C7) and lactic acid

The Analyst ◽

10.1039/an9840900179 ◽

1984 ◽

Vol 109 (2) ◽

pp. 179-180 ◽

Cited By ~ 5

Author(s):

A. T. G. Steverink

Keyword(s):

Fatty Acids ◽

Lactic Acid ◽

Volatile Fatty Acids ◽

Chromatographic Method ◽

Gas Chromatographic Method

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Determination of the activity coefficients of methanol and ethanol in benzene solutions by gas chromatography

Canadian Journal of Chemistry ◽

10.1139/v67-500 ◽

1967 ◽

Vol 45 (24) ◽

pp. 3089-3095 ◽

Cited By ~ 7

Author(s):

Peter Pollak ◽

G. C. B Cave

Keyword(s):

Gas Chromatography ◽

Quantitative Analysis ◽

Activity Coefficients ◽

Vapor Phase ◽

Chromatographic Method ◽

Low Concentrations ◽

Gas Chromatographic Method

A rapid gas-chromatographic method is described for the quantitative analysis of the equilibrium vapor phase over a solution. A vapor pump has been designed for the purpose and is described. The method should be particularly useful at low concentrations of volatile solutes, alone or in mixtures. As an example of the application of the method, the vapor concentrations of methanol and of ethanol over benzene solutions at 25 °C have been measured. The precision of the data is also reported. The activity coefficients of the alcohols in the benzene solutions were calculated.

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