CEA-Like Material in Fluid from Benign Cysts of the Breast

1974 ◽  
Vol 20 (1) ◽  
pp. 41-42 ◽  
Author(s):  
Martin Fleisher ◽  
Herbert F Oettgen ◽  
Charles N Breed ◽  
Guy F Robbins ◽  
Carl M Pinsky ◽  
...  

Abstract A preliminary survey of carcinoembryonic antigen (CEA) concentrations in 23 breast-cyst fluids (BCF) and blood plasma has revealed markedly increased concentrations of CEA-like material in BCF as compared to its concentrations in plasma. Chromatography on Sephadex G-200 indicated the immunoreactive CEA component extracted from BCF corresponds to a glycoprotein with a molecular weight of at least 200 000.

RSC Advances ◽  
2021 ◽  
Vol 11 (27) ◽  
pp. 16339-16350
Author(s):  
Mengkui Ding ◽  
Ling Zha ◽  
Hui Wang ◽  
Jinyao Liu ◽  
Peiwu Chen ◽  
...  

Novel frogspawn-like Ag@C nanoparticles were successfully used to fabricate an ultrasensitive electrochemical immunosensing platform toward CEA in human blood samples.


1996 ◽  
Vol 38 (3) ◽  
pp. 247-252 ◽  
Author(s):  
Ferdinando Mannello ◽  
GianDomenico Bocchiotti ◽  
Giuseppe Bianchi ◽  
Francesco Marcheggiani ◽  
Giancarlo Gazzanelli

1990 ◽  
Vol 586 (1 Biochemistry) ◽  
pp. 60-69 ◽  
Author(s):  
W. R. MILLER ◽  
W. N. SCOTT ◽  
R. W. KELLY ◽  
R. A. HAWKINS

THE BULLETIN ◽  
2021 ◽  
Vol 2 (390) ◽  
pp. 12-17
Author(s):  
A. Grozina

The research aimed to determine the effect of a mixture of low molecular weight organic acids and complex phytobiotics when replacing a feed antibiotic with them on the activity of digestive enzymes in the duode-nal chyme and the activity of pancreatic enzymes in the blood plasma of young stock B5 and B9 meat chicken lines. The experiments were carried out on the original lines of meat poultry lines B5 (Cornish) and B9 (Plymouth rock). There was an operation to insert a cannula into the duodenum at the age of 6 weeks. The enzymatic activity of the duodenum chyme and the content of pancreatic enzymes in the blood plasma in the groups of chickens receiving antibiotics, low molecular weight organic acids, and phytobiotics with the diet. The data showed that the influence of feed additives on the physiological status of poultry was different. The use of low molecular weight organic acids of the B5 and B9 chicken lines had a significant effect on the production of digestive enzymes due to an increase in the activity of chyme lipase (by 98.3%) and blood plasma lipase (by 26.6%) in B9 chickens and an increase in chyme proteases (by 30.9%) in B5 chickens compared with the control group, where the antibiotic was used. The introduction of complex phytobiotics into the diet had a negative effect on chickens of the B5 line (Cornish), reducing the activity of amylase and lipase of the duodenal chyme (by 29.2 and 26.9%) compared with the control group. In B9 (Plymouth rock) chickens, only the chyme amylase activity increased by 30.8% that indicates an improvement in the availability of feed carbohydrates. These data confirm the need to take into account the different effects of feed additives on the digestion processes in different poultry crosses.


2019 ◽  
Vol 26 (4) ◽  
pp. 56-65
Author(s):  
М. M. Tlish ◽  
Е. К. Popandopulo

Aim. The paper studies the dynamics of the indicators defi ning the antioxidant system (AOS) and endogenous intoxication in patients with microbial eczema (ME) prior to and following treatment.Materials and methods. The experimental group consisted of patients with ME (n=30) and the control group comprising somatically healthy individuals (n=30). In this study, we determined the components of the antioxidant defence system in blood plasma and erythrocyte suspension, as well as assessed endogenous intoxication.Results. The study revealed an imbalance of indicators refl ecting the severity of oxidative stress and endogenous intoxication in patients suffering from ME. Prior to receiving treatment, the experimental group, as compared to the control group, exhibited an increase in catalase activity (CAT) up to 35.93 [32.50; 38.22] mmol / (min • l) (p < 0.001) and glutathione peroxidase (GPx) up to 1194.27 [1069.50; 1375.78] μmol / (min • l) (p < 0.001). In addition, there was a decrease in the activity of superoxide dismutase (SOD) to 13.28 [11.35; 14.10] u/l (p < 0.001), glutathione reductase (GR) to 501.46 [479.77; 542.52] mmol / (min • l) (p < 0.001) and the concentration of reduced glutathione to 1.64 [1.59; 1.78] μmol / ml (p = 0.043). We observed no statistically signifi cant changes in the total antioxidant activity (TAA) of blood plasma (0.59 [0.55;0.62] mg/l; р = 0.375) and the sulphhydryl groups (0.44 [0.41;0.47] ODU; р =0.111). The patients with ME exhibited the syndrome of endogenous intoxication (SEI), accompanied by an increase in the content of medium and low molecular weight molecules in plasma to 12.19 [11.20; 13.37] arb. u. (p < 0.001), with their content in erythrocytes being relatively normal – 15.23 [14.26; 16.44] arb. u. (p = 0.005). Following the treatment, changes in the studied parameters were of a multidirectional character in relation to the control values. An increased level of CAT and GPx (р <0,001), as well as lower values of SOD and GR, continued to be observed (р < 0.001). The TAA in serum (p = 0.308), sulphhydryl groups (p = 0.111) and reduced glutathione (p = 0.107) did not differ signifi cantly from the control values. The treatment did not reverse the SEI: the content of medium and low molecular weight molecules continued to increase in both plasma and erythrocytes, as compared to the control values (p < 0.001 in both cases).Conclusion. The obtained data confi rm the failure of mechanisms underlying antioxidant defence in patients with ME. Despite the attempt of its compensation by increasing the catalase activity, SOD control values were still not reached. An increase in the GPx activity along with a decrease in GR was observed, which could have lead, in turn, to a decrease in the concentration of reduced glutathione. The failure of the antioxidant defence mechanisms is also indicated by an increase in the content of medium and low molecular weight molecules, as well as by the development of the SEI, resulting from the excessive formation of reactive oxygen species and the inability of the antioxidant system to neutralise them.


1955 ◽  
Vol 33 (1) ◽  
pp. 448-452 ◽  
Author(s):  
Irving Levi ◽  
Ezra Lozinski

It was observed that when very dilute solutions of hydrogen peroxide were employed for the purpose of destroying pyrogens in aqueous dextran solutions, a marked depolymerization of the polysaccharide molecule occurred. Further study demonstrated this effect to occur over a wide range of temperature, pressure, time, and concentration of hydrogen peroxide. It was therefore possible to use hydrogen peroxide in place of the usual hydrolytic agents, such as acids, for the depolymerization of native dextran to smaller molecular weight fragments suitable for use as a blood plasma extender.


1991 ◽  
Vol 27 (1) ◽  
pp. 44-47 ◽  
Author(s):  
G. Secreto ◽  
C. Recchione ◽  
P. Ballerini ◽  
L. Callegari ◽  
A. Cavalleri ◽  
...  
Keyword(s):  

1999 ◽  
Vol 57 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Manuela Malatesta ◽  
Ferdinando Mannello ◽  
Maurizio Sebastiani ◽  
Giuseppe Bianchi ◽  
Giancarlo Gazzanelli

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