Effect of the Matrix of the Standard on Results of Atomic Absorption Spectrophotometry of Zinc in Serum

1975 ◽  
Vol 21 (4) ◽  
pp. 588-590 ◽  
Author(s):  
Berlslav Momčilović ◽  
Bartholomeus Belonje ◽  
Bhagwan G Shah
Keyword(s):  
Nitric Acid ◽  
Human Serum ◽  
Atomic Absorption ◽  
Physiological Saline ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry ◽  
Flame Atomic Absorption ◽  
The Matrix ◽  
The Mean ◽  
Synthetic Serum

Abstract Zinc in pooled human serum, diluted 10-fold with demineralized water, was determined by flame atomic absorption spectrophotometry. Recoveries of zinc added to the serum were calculated by use of standards prepared in water, physiological saline, synthetic serum, or dilute HNO3 (10 ml/liter). The flow rates were essentially the same (9.1 to 9.3 ml/min) for water, diluted synthetic serum or actual human serum, and diluted nitric acid. The mean apparent percentage recoveries (±SD) were the best (102.6 ± 2.5) when the standards were prepared in nitric acid; the values obtained when standards in water were used were too high (111.5 ± 3.9); those with standards prepared in physiological saline (92.0 ± 3.2) or synthetic serum (92.3 ± 2.5) were too low.

Optimized atomic absorption spectrophotometry of calcium in erythrocytes.

1987 ◽  
Vol 33 (11) ◽  
pp. 2004-2007 ◽  
Author(s):  
S Nomoto ◽  
S Shoji
Keyword(s):  
Hydrochloric Acid ◽  
Atomic Absorption ◽  
Atomic Absorption Spectrophotometry ◽  
Graphite Tube ◽  
Optimum Conditions ◽  
Absorption Spectrophotometry ◽  
Flame Atomic Absorption ◽  
Flame Atomic Absorption Spectrophotometer ◽  
The Mean ◽  
Mean Concentration

Abstract We sought to establish optimum conditions for measuring calcium in erythrocytes by atomic absorption spectrophotometry. The conditions we selected are as follows. Wash one volume of fresh heparin-treated packed cells once with 30 volumes of isotonic buffered saline (pH 7.4) at a temperature somewhat exceeding 25 degrees C. Dilute the washed packed cells 10-fold with 12 mmol/L hydrochloric acid, and analyze the supernate for calcium. Measure the hematocrit of the washed packed cells, then analyze an aliquot of them for calcium, using a computer-readout type of flame or a non-flame atomic absorption spectrophotometer equipped with a pyrocoated graphite tube. The temperature program is 1000 degrees C for ashing [corrected] and 1800 degrees C for the atomizing cycle. Intraday and day-to-day reproducibility of the assay was 6.55% and 8.19%, respectively, at the mean concentration of calcium in the erythrocytes of healthy adults, which is 4.30 mumol/L.

Zinc in plasma, neutrophils, lymphocytes, and erythrocytes as determined by flameless atomic absorption spectrophotometry.

1982 ◽  
Vol 28 (3) ◽  
pp. 475-480 ◽  
Author(s):  
R C Whitehouse ◽  
A S Prasad ◽  
P I Rabbani ◽  
Z T Cossack
Keyword(s):  
Nitric Acid ◽  
Atomic Absorption ◽  
Matrix Effects ◽  
Atomic Absorption Spectrophotometry ◽  
Conventional Technique ◽  
Flame Atomic Absorption Spectrophotometry ◽  
Sample Matrix ◽  
Flameless Atomic Absorption ◽  
Absorption Spectrophotometry ◽  
Flame Atomic Absorption

Abstract Zinc is determined in neutrophils and lymphocytes (isolated from whole blood on discontinuous gradients of Ficoll-Hypaque) and in microliter quantities of plasma and erythrocytes by flameless atomic absorption spectrophotometry with greater sensitivity than with conventional flame atomic absorption spectrophotometry. Before analysis, neutrophils and lymphocytes are digested with nitric acid and diluted with de-ionized water. Plasma and erythrocytes required no digestion, only dilution. Overall CVs were 4.0, 3.0, 5.0, and 4.6% for neutrophils, lymphocytes, erythrocytes, and plasma, respectively. Matrix effects were fully compensated for by use of standard solutions that simulated the sample matrix. Results for plasma and erythrocytes agreed with those obtained by the conventional technique.

Optimized atomic absorption spectrophotometry of zinc in cerebrospinal fluid.

1983 ◽  
Vol 29 (3) ◽  
pp. 486-491 ◽  
Author(s):  
R Palm ◽  
R Sjöström ◽  
G Hallmans
Keyword(s):  
Cerebrospinal Fluid ◽  
Atomic Absorption ◽  
Direct Determination ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry ◽  
Flame Atomic Absorption ◽  
Zn Concentration ◽  
Insignificant Difference ◽  
The Mean

Abstract This method for direct determination of Zn in cerebrospinal fluid (CSF) involves flame atomic absorption spectrophotometry with a pulse nebulizer technique. Standard solutions of Zn in 150 mmol/L NaCl were used. We could account for 88% of added standard with the method in individual samples from 10 patients and in pooled CSF. The method is acceptably precise, CVs in pooled CSF ranging from 4 to 12%. The mean CSF-Zn concentration for nine healthy men was 0.18 (SD 0.04) mumol/L and for nine healthy women 0.15 (SD 0.03) mumol/L, a statistically insignificant difference. These values are lower than those in previous reports, which may have been the result of contamination problems, nonatomic absorption, or nonstandardized sampling. In the healthy volunteers, the CSF-Zn concentration was positively correlated with serum-Zn, CSF-protein, and CSF-albumin concentrations, as well as with the CSF/serum ratio for albumin.

Reliable determination of copper complex ions in synthetic wastewater using flame atomic absorption spectrophotometry

2019 ◽  
Vol 79 (5) ◽  
pp. 833-841 ◽  
Author(s):  
Kanokporn Supong ◽  
Parnuwat Usapein
Keyword(s):  
Atomic Absorption ◽  
Copper Complexes ◽  
Atomic Absorption Spectrophotometry ◽  
Synthetic Wastewater ◽  
Flame Atomic Absorption Spectrophotometry ◽  
Working Standard ◽  
Absorption Spectrophotometry ◽  
Flame Atomic Absorption ◽  
Relative Standard ◽  
Delay Times

Abstract Forms of copper that are highly soluble in aquatic environments are used as chemical reagents in a variety of industries, especially copper complexes. Wastewater containing copper complexes can be difficult to treat and analyse. Normally, flame atomic absorption spectrophotometry (FAAS) is a favourable technique for analysing metal ions in wastewater, but is less reliable for copper analysis owing to the influence of copper oxides. To solve this problem, it is necessary to adjust the atomization and delay times. The objective of this study was to use FAAS to accurately determine the quantity of complexed copper in synthetic wastewater, using increased atomization and delay times. The method showed excellent linearity in the copper standard concentration range of 1–5 mg L−1. The sensitivity of the analysis was 0.023 mg L−1. The percentage of recovery and the relative standard deviation were 85.02% and 0.75%, respectively. The experimental results demonstrated that the estimation of uncertainty from preparation of working standard, repeatability uncertainty, instrument deviation, calibration curve and recovery uncertainty were 8.2 × 10−4, 4.49 × 10−4, 7.21 × 10−4, 21.25 × 10−4, and 98.19 × 10−4, respectively. Overall, the results showed the suitability of the FAAS method for determining copper in synthetic wastewater.

Influence of Phosphorus and Calcium on Flame Atomic Absorption Spectrophotometric Determination of Lead in Canned Fish Products

1984 ◽  
Vol 67 (1) ◽  
pp. 186-187
Author(s):  
Teresa M Serra ◽  
J F Vale Serrano
Keyword(s):  
Atomic Absorption ◽  
Atomic Absorption Spectrophotometry ◽  
Flame Atomic Absorption Spectrophotometry ◽  
Experimental Conditions ◽  
Fish Products ◽  
Real Samples ◽  
Absorption Spectrophotometry ◽  
Flame Atomic Absorption ◽  
Canned Fish

Abstract Lead was determined in the presence of whole multiples of the P/Ca ratio found in Portuguese canned fish by flame atomic absorption spectrophotometry with and without using an ashing aid. Under our experimental conditions, use of the ashing aid eliminates P and Ca interference. Results with real samples, spiked with 1, 2,3, and 4 ppm lead, are presented and statistically treated.

A sensitive high-temperature electrothermal atomic absorption analysis for Rb+ in erythrocytes and plasma of normal and hypertensive persons.

1985 ◽  
Vol 31 (2) ◽  
pp. 274-276 ◽  
Author(s):  
K F Hallis ◽  
N A Boon ◽  
C M Perkins ◽  
J K Aronson ◽  
D G Grahame-Smith
Keyword(s):  
Atomic Absorption ◽  
Healthy Subjects ◽  
Graphite Furnace ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Analysis ◽  
Healthy Individuals ◽  
Absorption Spectrophotometry ◽  
Graphite Furnace Atomic Absorption ◽  
The Matrix

Abstract This method for determination of Rb+ in human plasma and erythrocytes by graphite-furnace atomic absorption spectrophotometry has a sensitivity of 29 nmol/L for plasma, 12 nmol/L for erythrocytes. The detection limit is 24 nmol/L for plasma, 4.8 nmol/L for erythrocytes. This assay is approximately 30-fold more sensitive than previously reported techniques involving atomic absorption spectrophotometry, enabling use of smaller samples. The rubidium signal is linear with concentration up to 1.2 mumol/L, and addition of other cations to the matrix produces only minor alterations in the Rb+ signal. We measured plasma and erythrocytic Rb+ concentrations in healthy subjects and in patients with untreated essential hypertension. In both, our values are similar to those previously reported for healthy individuals.

Precautions in Ashing Techniques for Lead Determination in Food

1982 ◽  
Vol 65 (4) ◽  
pp. 992-993
Author(s):  
Wayne Thornburg
Keyword(s):  
Atomic Absorption ◽  
Atomic Absorption Spectrophotometry ◽  
Lead Determination ◽  
Absorption Spectrophotometry ◽  
Dry Ashing ◽  
The Matrix ◽  
Wet Ashing

Abstract In determining lead in food, the matrix must be destroyed before quantitation by atomic absorption spectrophotometry. Wet or dry ashing techniques are generally used. Dry ashing assisted by ultrapure H2SO4 or K2SO4 has the advantages of safety, low reagent blanks, rapidity, and good recoveries of added lead. These techniques are also less laborintensive than wet ashing.

Microwave Acid Digestion of Various Food Matrixes for Nutrient Determination by Atomic Absorption Spectrophotometry

1991 ◽  
Vol 74 (5) ◽  
pp. 812-814 ◽  
Author(s):  
Philip J Oles ◽  
Wanda M Graham
Keyword(s):  
Atomic Absorption ◽  
Microwave Digestion ◽  
Manganese Content ◽  
Control Sample ◽  
Atomic Absorption Spectrophotometry ◽  
Breakfast Cereal ◽  
Hot Plate ◽  
Absorption Spectrophotometry ◽  
Flame Atomic Absorption ◽  
Microwave Apparatus

Abstract Food matrixes were digested by treatment with nitric acidsulfuric acid-hydrogen peroxide mixtures and heating with a commercially available microwave apparatus. The digests were analyzed for sodium, potassium, magnesium, iron, calcium, zinc, copper, and manganese content by flame atomic absorption spectrophotometry. A total of 3 digestion programs were adopted for a variety of food matrixes. Microwave digestion times of 30-45 mln were normally sufficient for obtaining clear digests compared to hot plate digestion times of 8-12 h. Precision for microwave digestion of most nutrients was equal to or better than that of hot plate digestion. Microwave digestion gave comparable results for the nutrient elements. National Institute of Standards and Technology reference materials were analyzed for nutrient content after microwave digestion and the results compared favorably with the certified values. A laboratory breakfast cereal control sample with a history of over 20 separate hot plate digestions was prepared using the microwave technique. Results for the 8 elements were within ±7% of the hot plate digestion values. Predlgestlon spikes were recovered from several food matrixes with no effects resulting from Incomplete digestion of samples.

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