Determination of Urinary Total Protein by Use of Gel Filtration and a Modified Biuret Method

1975 ◽  
Vol 21 (6) ◽  
pp. 778-781 ◽  
Author(s):  
Karl Doetsch ◽  
Richard H Gadsden
Keyword(s):  
Total Protein ◽  
Gel Filtration ◽  
Biuret Method

Concentration of dipotassium ethylenediaminetetraacetic acid but not lithium heparin affects total protein determination in equine synovial fluid

2020 ◽  
Vol 187 (8) ◽  
pp. e62-e62
Author(s):  
Pablo Jimenez Rihuete ◽  
Nicolas Villarino ◽  
Alicja Pelisiak ◽  
Luis M Rubio-Martinez
Keyword(s):  
Synovial Fluid ◽  
Cross Section ◽  
Total Protein ◽  
Gold Standard ◽  
Ethylenediaminetetraacetic Acid ◽  
Protein Determination ◽  
Collection Tube ◽  
Refractometric Determination ◽  
Biuret Method

BackgroundRefractometric determination of total protein (TP) in synovial fluid (SF) is commonly used for diagnosis and monitoring of synovial sepsis in horses. Previous studies have shown that elevated concentrations of certain anticoagulants may overestimate refractometric determination of TP concentration.ObjectivesThe aim of the study was to evaluate the effect of different concentrations of dipotassium EDTA (K2EDTA) and lithium heparin (LH) on TP determination by using a hand-held refractometer in equine synovial fluid.Study designCross-section observational study.MethodsThirty samples of synovial fluid obtained from 22 horses with different synovial conditions were collected. Synovial fluid samples were separated into different aliquots and placed in commercially available collection tubes containing K2EDTA or LH at four different concentrations (1.76, 3.52, 7.04 and 17.6 mg/ml for K2EDTA; 16, 32, 64 and 160 IU/ml for LH) . Refractometric TP determination was performed on untreated and K2EDTA and LH aliquots with a hand-held refractometer and by spectophotometric Biuret method as the gold standard.ResultsRefractometric TP determination was overestimated in SF samples containing 10 times the recommended K2EDTA concentrations. Lower concentrations of K2EDTA and LH concentrations did not affect refractometric TP determinations.Main limitationsLimited number of samples mostly obtained from large synovial structures.ConclusionTo avoid incorrect TP determination, the use of LH containing collection tubes may be an appropriate alternative when the SF volume available is not enough to fill the K2EDTA collection tube.

Automated multiple flow-injection analysis in clinical chemistry: determination of total protein with Biuret reagent.

1980 ◽  
Vol 26 (10) ◽  
pp. 1454-1458 ◽  
Author(s):  
C E Shideler ◽  
K K Stewart ◽  
J Crump ◽  
M R Wills ◽  
J Savory ◽  
...  
Keyword(s):  
Flow Injection ◽  
Total Protein ◽  
Clinical Chemistry ◽  
Injection Technique ◽  
Flow Rates ◽  
Pressure Injection ◽  
Relative Standard ◽  
Biuret Method ◽  
High Pressure Injection

Abstract We have examined the feasibility of the automated multiple flow-injection technique for application to clinical chemistry by adapting to this system the biuret method for the determination of total protein. Samples were discretely and rapidly introduced into a continuously flowing, nonsegmented reagent stream by means of an automatic sampler and high-pressure injection valve. Pumps operating at 1380-2070 kPa (200-300 psi) were utilized to introduce the biuret reagent and saline diluent into the system separately at flow rates of 72 and 47 microL/s, respectively. Use of 20-microL sample and a 3.0-s reaction-delay coil was adequately sensitive for analysis for total protein by this method. Samples were analyzed at a rate of 150/h with no detectable between-sample carryover. Within-run precision studies yielded relative standard deviations of 2.5% and less. Total protein values obtained by this method correlated well with those obtained by centrifugal analyzer and bubble-segmented continuous-flow biuret methods.

scholarly journals Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods

2005 ◽  
Vol 48 (3) ◽  
pp. 385-388 ◽  
Author(s):  
Dimas Augusto Morozin Zaia ◽  
Fábio Rangel Marques ◽  
Cássia Thaïs Bussamra Vieira Zaia
Keyword(s):  
Comparative Study ◽  
Blood Plasma ◽  
Total Protein ◽  
Total Proteins ◽  
Erythrosin B ◽  
Spectrophotometric Methods ◽  
Biuret Method ◽  
Bradford Method ◽  
In Blood Plasma

A comparative study between the biuret method (standard method for total proteins) and spectrophotometric methods using dyes (Bradford, 3',3",5',5"-tetrabromophenolphthalein ethyl ester-TBPEE, and erythrosin-B) was carried out for the determination of total proteins in blood plasma from rats. Bradford method showed the highest sensitivity for proteins and biuret method showed the lowest. For all the methods, the absorbance for different proteins (BSA, casein, and egg albumin) was measured and Bradford method showed the lowest variation of absorbance. The concentration of total protein obtained by using Bradford method was not statistically different (p>0.05) from concentration of total protein obtained by the biuret method. But in regard to erythrosin-B and TBPEE methods the concentrations of total protein were statistically different (p<0.05). Thus, Bradford method could be used instead of the biuret method for determination of total proteins in blood plasma.

scholarly journals DYNAMICS OF SERUM PROTEIN DURING THE ESTROUS CYCLE OF GOATS BRED IN BRAZIL AND NATURALLY INFECTED BY CAPRINE ARTHRITIS ENCEPHALITIS VIRUS

2006 ◽  
Vol 73 (1) ◽  
pp. 41-44
Author(s):  
F.C. Cyrillo ◽  
M.L. do R. Leal ◽  
F.J. Benesi ◽  
A.M.M. de P. Della Libera
Keyword(s):  
Estrous Cycle ◽  
Serum Protein ◽  
Total Protein ◽  
Estrus Cycle ◽  
Agar Gel ◽  
Caprine Arthritis Encephalitis Virus ◽  
Blood Samples ◽  
Biuret Method ◽  
Alpha Beta

ABSTRACT The aim of the present study was to evaluate variations in proteinogram occurring during the estrus cycle of animals infected or not by caprine arthritis encephalitis. Forty blood samples were collected from female goats in different phases of the estrus cycle (estrus, proestrus, metaestrus and diestrus). Samples were classified as positive (n = 5) and negative (n = 5), according to the results of the survey of antibodies against caprine arthritis encephalitis as performed by immunodiffusion in agar gel. Samples were also used in the determination of albumin, total protein and alpha, beta and gammaglobulin by means of electrophoresis and biuret method, respectively. Electrophoresis showed that estrus, proestrus, metaestrus and diestrus positive animals presented total protein mean equal to 7.96 ± 0.69, 7.67 ± 1.14, 7.77 ± 0.36, 7.48 ± 0.83; mean albumin equal to 3.22 ± 0.41, 3.11 ± 0.68, 3.30 ± 0.67, 3.28 ± 0.57; mean alphaglobulin equal to 0.70 ± 0.09, 0.70 ± 0.12, 0.69 ± 0.12, 0.61 ± 0.11; mean betaglobulin 1 equal to 0.85 ± 0.04, 0.87 ± 0.04, 0.90 ± 0.12, 0.83 ± 0.11; mean betaglobulin 2 equal to 0.77 ± 0.18, 0.68 ± 0.17, 0.60 ± 0.15, 0.54 ± 0.09; and mean gammaglobulin equal to 2.43 ± 0.47, 2.31 ± 0.54, 2.28 ± 0.49, 2.21 ± 0.54. Negative animals presented mean total protein equal 7.76 ± 1.31, 8.03 ± 1.10, 7.50 ± 0.51, 6.49 ± 1.14; mean albumin equal to 3.06 ± 0.46, 3.19 ± 0.46, 3.03 ± 0.62, 2.93 ± 0.96; mean alphaglobulin equal to 0.69 ± 0.19, 0.75 ± 0.18, 0.70 ± 0.24, 0.58 ± 0.20; mean betaglobulin 1 equal to 0.79 ± 0.03, 0.79 ± 0.23, 0.80 ± 0.15, 0.66 ± 0.16; mean betaglobulin 2 equal to 0.67 ± 0.20, 0.95 ± 0.37, 0.81 ± 0.40, 0.73 ± 0.41; and mean gammaglobulin equal to 2.35 ± 1.42; 2.42 ± 1.30, 2.23 ± 1.03, 1.92 ± 0.70, respectively. Variance analysis did not show any statistically significant differences. Animals infected by caprine arthritis encephalitis (CAE) did not present any changes in proteinogram, regardless of the phase of the estrus cycle they were in.

Determination of total protein in human blood by laser

2017 ◽  
Vol 13 (3) ◽  
pp. 261-274
Author(s):  
Sahra Salem Jassem ◽  
Keyword(s):  
Human Blood ◽  
Total Protein
Sign in / Sign up

Export Citation Format

Share Document