Importance of simultaneous determination of coproporphyrin and hemoglobin in contaminated amniotic fluid.

1986 ◽  
Vol 32 (5) ◽  
pp. 877-878 ◽  
Author(s):  
J Francoual ◽  
A Lindenbaum ◽  
C Benattar ◽  
M Dehan ◽  
H Cohen ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Simultaneous Determination ◽  
Biochemical Analysis ◽  
Reliable Method ◽  
Visual Examination ◽  
Mean Values ◽  
The Mean ◽  
Comparison Of The Results

Abstract To detect the presence of meconium and blood, we determined coproporphyrin and hemoglobin in 78 amniotic fluid samples, classified according to their visual aspect as clear (n = 45) or meconium-stained (n = 33). Among the 33 samples visually judged as meconial, a comparison of the results of their biochemical analysis with the mean values for clear amniotic fluids showed that only five of the 33 were contaminated solely with meconium. Seven others were exclusively bloody, and 19 were meconial and bloody. In two, the contaminant was neither meconium nor blood. Evidently, when the fluid is brownish, simple visual examination does not suffice for distinguishing meconium from old blood or other pigments. We propose a simple, rapid, and reliable method for simultaneously measuring contamination with meconium or blood.

SENSITIVE AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY FOR UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN HUMAN PLASMA

1987 ◽  
Author(s):  
J Grøndahl-HANSEN ◽  
N Agerlin ◽  
L S Nielsen ◽  
K Danø
Keyword(s):  
Plasminogen Activator ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mean Values ◽  
Amino Terminal ◽  
Type Plasminogen Activator ◽  
Healthy Donors ◽  
Urokinase Type Plasminogen Activator ◽  
The Mean

An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human urokinase-type plasminogen activator (u-PA) in plasma and serum. Microtiter plates were coated with a monoclonal antibody and incubated with standard or sample. Bound u-PA was quantitated with polyclonal antibodies conjugated with biotin, followed by avidin-peroxidase. The assay was 10-fold as sensitive as other previously reported ELISAs, the detection limit being approximately 1 pg of u-PA in a volume of 100 μl with a linear dose-response up to 15 pg of u-PA. The assay detected active u-PA and its inactive proenzyme form equally well and the recovery of both forms was higher than 90% in plasma. A variety of structurally related proteins, including t-PA, were tested, but no reaction with proteins other than u-PA and its amino-terminal degradation product were observed. The intra-assay and inter-assay coefficients of variation for determination of u-PA in plasma were 7.6% and 8.4%, respectively. The assay was equally applicable to serum. The values obtained with plasma and serum were similar, and the results were not affected by small variations in the preparation of the samples. The ELISA was used to measure the concentration of u-PA in plasma from 34 healthy donors. The mean values for u-PA in plasma from healthy donors was 1.1 ng/ml ± 0.3 ng/ml (SD) (range 0.6 - 1.5 ng/ml). No significant differences were found between men and women and no correlation between u-PA concentration and age could be demonstrated.The mean u-PA concentration in plasma from healthy donors obtained in this study is substantially lower than that reported by others. This might be due to different methods of determination of the protein content of the standard preparations or to differences in the specificity of the assays.

scholarly journals pH of exudate test in the physiological quality of crambe seeds

2016 ◽  
Vol 46 (6) ◽  
pp. 1014-1018 ◽  
Author(s):  
Charline Zaratin Alves ◽  
Lennis Afraire Rodrigues ◽  
Carlos Henrique Queiroz Rego ◽  
Josué Bispo da Silva
Keyword(s):  
Pearson Correlation ◽  
Raw Material ◽  
Mean Values ◽  
Tukey Test ◽  
Physiological Quality ◽  
The Subject ◽  
The Mean ◽  
C 30

ABSTRACT: Crambe is a rapeseed with high oil content and can be used as a winter cover or as a source of raw material for the production of biodiesel, however espite the growing interest in the culture, research on the subject is still incipient, especially concerning the seed production and analysis technology. The purpose of this study is to evaluate the physiological quality of crambe seeds, 'FMS Brilhante' cultivar, by testing the pH of exudate. Five seed lots were submitted to the determination of water content and the tests of germination and vigor (first count, emergence and tetrazolium). In the conduction of pH exudate test, temperatures (25 and 30oC), and periods of seed imbibition in water (15, 30 and 45 minutes) were tested. The experiment was conducted in a completely randomized manner, with four replicates, and the mean values were compared by the Tukey test at 5% probability; Pearson correlation between the pH of the exudate and initial tests was also made. Testing the pH of exudate is promising for separating lots of crambe seeds and the following combinations of 25°C/30 minutes or 30°C/45 minutes can be used.

The Determination of the Probabilistic Properties of Velocities and Accelerations in Kinematic Chains With Uncertainty

1989 ◽  
Author(s):  
S. J. Lee ◽  
B. J. Gilmore
Keyword(s):  
Effective Length ◽  
Radial Clearance ◽  
Probabilistic Design ◽  
Link Length ◽  
Mean Values ◽  
Kinematic Chains ◽  
Analysis Package ◽  
The Mean ◽  
Linkage Model

Abstract A probabilistic model and methods to determine the means and variances of the velocity and acceleration of stochastically-defined planar pin jointed kinematic chains are presented. The presented model considers the effect of tolerances on link length and radial clearance and uncertainty of pin location as a net effect on the link’s effective length. The determination of the mean values and variances of the output variables requires the calculation of sensitivities of secondary variables with respect to the random variables. It is shown that this computation is straightforward and can be accomplished by a conventional kinematic analysis package. Thus, the concepts of tolerance and clearance have been captured by the model and analysis. The only input data is the nominal linkage model and statistical information. The “effective link length” model is shown to be applicable to both analytical solution and Monte Carlo simulation. The results from both methods are compared. This paper solves the higher-order kinematics problem for the probabilistic design analysis of stochastically defined mechanisms.

Sedimentation of lung-derived phospholipid during low-speed centrifugation of amniotic fluid.

1981 ◽  
Vol 27 (8) ◽  
pp. 1424-1426 ◽  
Author(s):  
C G Duck-Chong ◽  
L M Brown ◽  
W J Hensley
Keyword(s):  
Amniotic Fluid ◽  
Total Phospholipid ◽  
Gradient Centrifugation ◽  
Lamellar Body ◽  
Fetal Lung ◽  
Mean Values ◽  
Low Speed ◽  
Whole Cells ◽  
Appreciable Increase ◽  
The Mean

Abstract In most methods proposed for the assessment of fetal lung maturity, amniotic fluid is subjected to a preliminary low-speed centrifugation in an attempt to separate whole cells and cell debris from lung-derived surfactant phospholipid (lamellar body phospholipid). However, because lamellar body phospholipid is present in amniotic fluid in a membranous or particulate form, it is also partly sedimented by this procedure. The sedimentation of total phospholipid and lamellar body phospholipid by low-speed centrifugation has been quantitated for 49 samples of amniotic fluid from pregnancies of 30--41 weeks gestation. Isopycnic density-gradient centrifugation in a small air-driven ultracentrifuge was used to isolate lamellar body fractions from whole and centrifuged amniotic fluid. Centrifugation for 5 min at 1000 x g removed 0--70% of total phospholipid or lamellar body phospholipid, the mean values being 34 or 29%, respectively. There was an appreciable increase in lamellar body phospholipid relative to total phospholipid as a result of centrifugation in only 51% of the samples. In general, the effects of centrifugation were not related to gestational age of the fetus or the state of maturity of its lungs.

Colorimetric Method for Rapid Determination of Serum Arginase

1967 ◽  
Vol 13 (10) ◽  
pp. 900-908 ◽  
Author(s):  
Brigitta Mellerup
Keyword(s):  
Rapid Determination ◽  
Colorimetric Method ◽  
Normal Range ◽  
Clinical Routine ◽  
Mean Values ◽  
Enzymatic Formation ◽  
Significant Difference ◽  
The Mean ◽  
Normal Men

Abstract A method for the determination of serum arginase is given which combines the enzymatic formation of urea with the sensitive method of Coulombe (1) for measuring this substance. This procedure allows more accurate determinations in the normal range than do previous methods described and is convenient for clinical routine. Significant difference is found between the mean values of normal men and women, 3.9 units/L. for the former and 2.9 units/L. for the latter.

scholarly journals Determination of carcinoembryonic antigen and cancer antigen values with the radioimmunoassay method in healthy females dogs

2013 ◽  
Vol 58 (No. 5) ◽  
pp. 277-283 ◽  
Author(s):  
V. Ledecky ◽  
A. Valencakova-Agyagosova ◽  
J. Lepej ◽  
Z. Frischova ◽  
S. Hornak ◽  
...  
Keyword(s):  
Carcinoembryonic Antigen ◽  
Statistical Significance ◽  
Average Weight ◽  
Cancer Antigen ◽  
Cancer Markers ◽  
Mean Values ◽  
The Mean ◽  
Radioimmunoassay Method ◽  
Healthy Females

The aim of this study was to determine reference values of carcinoembryonic antigen and cancer antigen in 32 clinically healthy bitches. The average age of the bitches in each group was as follows: small breeds 3.50 &plusmn; 2.30, medium breeds 3.83 &plusmn; 3.21, large breeds 6.00 &plusmn; 3.22 and giant breeds 2.40 &plusmn; 2.43. The average weight in each group was as follows: 1<sup>st</sup> group 7.94 kg &plusmn; 1.84, 2<sup>nd</sup> group 22.38 kg &plusmn; 2.77, 3<sup>rd</sup> group 35.94 kg &plusmn; 7.16, and 4<sup>th</sup>&nbsp;group 52.75 kg &plusmn; 5.04. The cancer markers were determined using human kits. The mean values of the carcinoembryotic antigen markers &plusmn; SD were as follows: 1<sup>st</sup> group 0.18 &plusmn; 0.03, 2<sup>nd</sup> group 0.20 &plusmn; 0.03, 3<sup>rd</sup> group 0.22 &plusmn; 0.01, 4<sup>th</sup> group 0.18 &plusmn; 0.04. The statistical significance for the carcinoembryonic antigen markers was P = 0.0042**. The values of cancer antigen markers &plusmn; SD were: 4.90 &plusmn; 1.04, 4.80 &plusmn; 1.13, 5.90 &plusmn; 1.22, and 4.72 &plusmn; 0.97, respectively. The cancer antigen values were statistically insignificant (P = 0.1762). Based on obtained values of the mean 95%, we expect a standard for carcinoembryonic antigen of 0.00&ndash;0.23 ng/ml and for cancer antigen 0.0&ndash;7.00 IU/ml. The results of the present study show that it is possible to use human kits for the determination of carcinoembryonic antigen and cancer antigen in clinically healthy bitches using the radioimmunoassay method. &nbsp;

scholarly journals Zero order and area under curve spectrophotometric methods for determination of Levocetirizine in pharmaceutical formulation

2015 ◽  
Vol 1 (6) ◽  
pp. 270
Author(s):  
Audumbar Digambar Mali ◽  
Ritesh Bathe ◽  
Manojkumar Patil ◽  
Ashpak Tamboli
Keyword(s):  
Area Under The Curve ◽  
Zero Order ◽  
Pharmaceutical Dosage Forms ◽  
Mean Values ◽  
Spectrophotometric Methods ◽  
Ich Guidelines ◽  
Significant Difference ◽  
Curve Method ◽  
The Mean

Simple, fast and reliable spectrophotometric methods were developed for determination of Levocetirizine in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in methanol. The quantitative determination of the drug was carried out using the zero order derivative values measured at 230 nm and the area under the curve method values measured at 227-234 nm (n=2). Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Levocetirizine using 5-25?g/ml (r=0.998 and r=0.999) for zero order and area under the curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. Developed spectrophotometric methods in this study are simple, accurate, precise and sensitive to assay of Levocetirizine in tablets.

The Determination of the Probabilistic Properties of Velocities and Accelerations in Kinematic Chains With Uncertainty

1991 ◽  
Vol 113 (1) ◽  
pp. 84-90 ◽  
Author(s):  
S. J. Lee ◽  
B. J. Gilmore
Keyword(s):  
Effective Length ◽  
Radial Clearance ◽  
Probabilistic Design ◽  
Link Length ◽  
Mean Values ◽  
Kinematic Chains ◽  
Analysis Package ◽  
The Mean ◽  
Linkage Model

A probabilistic model and methods to determine the means and variances of the velocity and acceleration within stochastically-defined planar pin jointed kinematic chains are presented. The presented model considers the effect of tolerances on link length and radial clearance and uncertainty of pin location as a net effect on the link’s effective length. The determination of the mean values and variances of the output variables requires the calculation of sensitivities of secondary variables with respect to the random variables. It is shown that this computation is straightforward and can be accomplished by a conventional kinematic analysis package. Thus, the concepts of tolerance and clearance have been captured by the model and analysis. The only input data is the nominal linkage model and statistical information. The “effective link length” model is shown to be applicable to both analytical solution and Monte Carlo simulation. The results from both methods are compared. This paper solves the higher-order kinematics problem for the probabilistic design analysis of stochastically defined mechanisms.

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