pH of exudate test in the physiological quality of crambe seeds

ABSTRACT: Crambe is a rapeseed with high oil content and can be used as a winter cover or as a source of raw material for the production of biodiesel, however espite the growing interest in the culture, research on the subject is still incipient, especially concerning the seed production and analysis technology. The purpose of this study is to evaluate the physiological quality of crambe seeds, 'FMS Brilhante' cultivar, by testing the pH of exudate. Five seed lots were submitted to the determination of water content and the tests of germination and vigor (first count, emergence and tetrazolium). In the conduction of pH exudate test, temperatures (25 and 30oC), and periods of seed imbibition in water (15, 30 and 45 minutes) were tested. The experiment was conducted in a completely randomized manner, with four replicates, and the mean values were compared by the Tukey test at 5% probability; Pearson correlation between the pH of the exudate and initial tests was also made. Testing the pH of exudate is promising for separating lots of crambe seeds and the following combinations of 25°C/30 minutes or 30°C/45 minutes can be used.

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P6489Preoperative determination of artificial chordae length by transesophageal echocardiography

European Heart Journal ◽

10.1093/eurheartj/ehz746.1079 ◽

2019 ◽

Vol 40 (Supplement_1) ◽

Author(s):

A Pitsis ◽

N Tsotsolis ◽

E Theofilogiannakos ◽

H Boudoulas ◽

K Boudoulas

Keyword(s):

Mitral Valve ◽

Mitral Valve Repair ◽

Pearson Correlation ◽

Transoesophageal Echocardiography ◽

Valve Repair ◽

Bottom Part ◽

Artificial Chordae ◽

The Mean

Abstract The use of artificial chordae is one of the main techniques used in mitral valve repair to treat prolapsing leaflets, especially in anterior and bileaflet prolapse. With the use of PTFE sutures to replace elongated or ruptured chords mitral valve repair rates have significantly improved. The main difficulty with this technique is to determine the optimal length of the artificial chordae. Intraoperative transoesophageal echocardiography (TOE) is mandatory in mitral valve repair in order to determine the type of lesion of the mitral valve but also to evaluate the quality of the repair. We examined the accuracy of preoperative prediction of artificial chordae length by the preoperativeTOE. Patients and methods Twenty-one consecutive patients (7 females) underwent mitral valve repair with artificial chordae for significant mitral valve prolapse in our department during the last year. The median age of the patients was 62 y. (range 25 - 87) and the mean EuroSCORE II 3,36% (SD 4,61%). During the prep TOE we determined the predicted length of the required replacement chordae for the repair using mainly the 4 chamber view to calculate the distance between the tip of the papillary muscle and the coaptation point of the two leaflets and we subtracted 5mm which is the minimum of the desired coaptation length (top right part of the Figure). All the patients underwent totally endoscopic mitral repair through a 3 cm right periareolar incision. 14,3% of the patients had anterior leaflet (AML) prolapse, 23,8% bileaflet and 61,9% posterior (PML). The appropriate length of the required chordae was measured intraoperatively. Results We used on average 3 loops of artificial chordae size 23,3 mm (SD 1,15mm) to treat the AML prolapse, 5 loops size 23,2 mm (SD 2,28mm) to treat the bileaflet prolapse and 2,23 loops size 18mm (SD 2mm) to treat the PML prolapse (bottom part of the figure). The predicted size of the artificial chordae had a positive correlation to the length used (Pearson correlation, p<0,001) as demonstrated in the top left part of the Figure. An annuloplasty band was implanted to all the patients. All the patients had no mitral regurgitation in the postoperative TOE. The mean valve area was 3,54cm2 (SD 0,57) and the mean peak gradient 5,6 mmHg (SD 1,82). There was no mortality. Chordae length predicted by echo Conclusion The length of artificial chordae can be predetermined with great accuracy with the use of TOE, making TOE an important tool not only for the determination of the mitral lesion and quality of the repair but also for the planning of the operation.

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Content of toxic elements in tissues of hunted animals on the basis of research results of 2003–2017

Medycyna Weterynaryjna ◽

10.21521/mw.6202 ◽

2019 ◽

Vol 75 (02) ◽

pp. 6202-2019

Author(s):

MARIUSZ RUDY ◽

JAGODA ŻUREK ◽

RENATA STANISŁAWCZYK ◽

MARIAN GIL ◽

PAULINA DUMA-KOCAN

Keyword(s):

Wild Boar ◽

Muscle Tissue ◽

Toxic Elements ◽

Roe Deer ◽

Raw Material ◽

Cadmium Content ◽

Mean Values ◽

Wild Boars ◽

The Mean

The aim of the study was to synthesize the latest literature data on the content of toxic elements in tissues of hunted animals and to determine whether there is a health risk associated with consumption of these products in the light of current legal regulations. The data show the increased mean lead contents in kidneys of wild boar (1.1900 mg/kg) and roe deer (0.9060 mg/kg) and in the muscle tissue of wild boar, roe deer and deer; the contents are even up to 0.1240 mg/kg, 0.1760 mg/kg and 0.2200 mg/kg, respectively. In addition, the increased mean cadmium content indicates the possibility of the deposition of this element in muscle tissue of wild boars (0.0519 mg/kg) and deer (0.1000 mg/kg), as well as in the liver of wild boars (5.5730 mg/kg) and roe deer (6.4350 mg/kg) and in kidneys of game species, sometimes far above the applicable maximum levels. The highest mean cadmium contents in kidneys of wild boar, roe deer and deer are 49.5000 mg/kg, 80.2600 mg/kg and 4.9740 mg/kg, respectively. The mean values of the amount of arsenic do not usually exceed the legally established maximum levels of this element. Only the borderline value of arsenic in muscle tissue of roe deer is slightly exceeded and amounts to 0.2700 mg/kg. There is the possibility of the accumulation of larger amounts of mercury mainly in kidneys of deer and roe deer. The highest mean content of this element of 0.1001 mg/kg is demonstrated in the kidneys of deer. The research on the content of toxic elements in meat of hunted animals indicates the need to regularly monitor the quality of the obtained raw material as well as to exclude selected tissues of wild animals from consumption.

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An interlaboratory study of the determination of digoxin by immunoassay.

Clinical Chemistry ◽

10.1093/clinchem/26.3.0480 ◽

1980 ◽

Vol 26 (3) ◽

pp. 480-486

Author(s):

D M Fast ◽

W H Hannon ◽

C A Burtis ◽

D D Bayse

Keyword(s):

Frequency Distribution ◽

Statistical Difference ◽

Analytical Data ◽

Statistical Information ◽

Mean Values ◽

Clinical Laboratories ◽

The Mean ◽

Computational Procedures

Abstract We conducted a voluntary survey of laboratories and manufacturers to assess the current quality of analytical assays for serum digoxin. More than 300 clinical laboratories and 18 manufacturers responded, giving data on methods, instruments, computational procedures, and results for five survey samples. We sorted the analytical data to provide statistical information on the grand mean values separately for manufacturers and clinical laboratories, the frequency distribution of all reported values, and the mean values by method of interpolation and algorithms used for linear transformation. There was no statistical difference (alpha = 0.05) between the means for each specimen as determined by the kit manufacturers as a group and the clinical laboratories as a group.

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ASSESSMENT OF COLOR OF MEAT USING THE METHOD OF COMPUTER COLORIMETRY

EUREKA Life Sciences ◽

10.21303/2504-5695.2016.00141 ◽

2016 ◽

Vol 3 ◽

pp. 3-7 ◽

Cited By ~ 1

Author(s):

Oksana Petrusha ◽

Alexandra Niemirich

Keyword(s):

Standard Deviation ◽

Mean Value ◽

Raw Material ◽

Color Parameter ◽

Native State ◽

Digital Technique ◽

Color Coordinates ◽

The Mean

In the article is considered a possibility to use the accessible modern digital technique: flatbet scanners, digital photocameras and web-cameras for determination of color of foodstuff. The offered method allows get the digital image of studied sample and count information about the values of color coordinates of its every pixel that characterizes the color of meat half-finished product such as meat powder. The assessment of this raw material was carried out in dry and restored state. At measurement of color coordinates of the meat powders in native state there was determined the method of sample preparation for getting the mean value of color with the least standard deviation ~ 20 %. Thus, according to the studies, the sample of dry meat half-finished product must be reduced to fragments less than 0,2 mm. Availability of this method allows use it for assessment of the quality of dry meat half-finished products according to color parameter.

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Quality of Silages Made From Meadow Sward From South-Eastern Poland

Ecological Chemistry and Engineering S ◽

10.2478/eces-2020-0019 ◽

2020 ◽

Vol 27 (2) ◽

pp. 295-303

Author(s):

Adam Radkowski ◽

Iwona Radkowska ◽

Jan Bocianowski

Keyword(s):

Plant Material ◽

Weather Conditions ◽

Raw Material ◽

Elevated Concentration ◽

Crude Fibre ◽

Mean Values ◽

Nutrient Value ◽

Acid Detergent Fibre ◽

The Mean

AbstractWeather conditions prevailing in Poland often cause that meadow sward designated for silage is harvested too late, which decreases the quality of prepared silage. The aim of the research was to assess the quality of silages from dried meadow sward. The silages were ensiled in large cylindrical bales in selected individual farms specializing in milk production. The farms where the research was carried out were located in three voivodeships: slaskie, malopolskie and podkarpackie. In the prepared plant material, the basic chemical composition was determined using the method. When comparing the mean values, it was established that silages from the investigated region had a favourable content of total protein, the highest concentration was recorded for silages from Slask, followed by Malopolska and Podkarpacie. A slightly elevated concentration of crude fibre, fraction of acid detergent fibre (ADF) and neutral detergent fibre (NDF), was recorded. This fact shows that farmers collect raw material, particularly from the first cut, too late. Nutrient value of silages from meadow sward decreases with progressing vegetation. Higher protein and energy losses during sward ensiling were also observed at considerable drying of the plant material. In most cases, silages from the studied farms had a low content of monosaccharides. The carried out chemical analyses showed that in overall assessment the studied silages are of good quality; silages prepared from meadow sward from the third cut had the highest value. Proper technology of preservation of meadow sward is one of important factors in the production of feed for ruminants.

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An interlaboratory study of the determination of digoxin by immunoassay.

Clinical Chemistry ◽

10.1093/clinchem/26.3.480 ◽

1980 ◽

Vol 26 (3) ◽

pp. 480-486 ◽

Cited By ~ 4

Author(s):

D M Fast ◽

W H Hannon ◽

C A Burtis ◽

D D Bayse

Keyword(s):

Frequency Distribution ◽

Statistical Difference ◽

Analytical Data ◽

Statistical Information ◽

Mean Values ◽

Clinical Laboratories ◽

The Mean ◽

Computational Procedures

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The Quality of Statistical Reporting and Data Presentation in Predatory Dental Journals Was Lower Than in Non-Predatory Journals

Entropy ◽

10.3390/e23040468 ◽

2021 ◽

Vol 23 (4) ◽

pp. 468

Author(s):

Pentti Nieminen ◽

Sergio E. Uribe

Keyword(s):

Open Access ◽

Peer Review ◽

Scientific Journals ◽

Mean Values ◽

Data Presentation ◽

Predatory Journals ◽

Study Results ◽

The Mean ◽

Highly Cited

Proper peer review and quality of published articles are often regarded as signs of reliable scientific journals. The aim of this study was to compare whether the quality of statistical reporting and data presentation differs among articles published in ‘predatory dental journals’ and in other dental journals. We evaluated 50 articles published in ‘predatory open access (OA) journals’ and 100 clinical trials published in legitimate dental journals between 2019 and 2020. The quality of statistical reporting and data presentation of each paper was assessed on a scale from 0 (poor) to 10 (high). The mean (SD) quality score of the statistical reporting and data presentation was 2.5 (1.4) for the predatory OA journals, 4.8 (1.8) for the legitimate OA journals, and 5.6 (1.8) for the more visible dental journals. The mean values differed significantly (p < 0.001). The quality of statistical reporting of clinical studies published in predatory journals was found to be lower than in open access and highly cited journals. This difference in quality is a wake-up call to consume study results critically. Poor statistical reporting indicates wider general lower quality in publications where the authors and journals are less likely to be critiqued by peer review.

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SENSITIVE AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY FOR UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN HUMAN PLASMA

10.1055/s-0038-1644425 ◽

1987 ◽

Cited By ~ 1

Author(s):

J Grøndahl-HANSEN ◽

N Agerlin ◽

L S Nielsen ◽

K Danø

Keyword(s):

Plasminogen Activator ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Mean Values ◽

Amino Terminal ◽

Type Plasminogen Activator ◽

Healthy Donors ◽

Urokinase Type Plasminogen Activator ◽

The Mean

An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human urokinase-type plasminogen activator (u-PA) in plasma and serum. Microtiter plates were coated with a monoclonal antibody and incubated with standard or sample. Bound u-PA was quantitated with polyclonal antibodies conjugated with biotin, followed by avidin-peroxidase. The assay was 10-fold as sensitive as other previously reported ELISAs, the detection limit being approximately 1 pg of u-PA in a volume of 100 μl with a linear dose-response up to 15 pg of u-PA. The assay detected active u-PA and its inactive proenzyme form equally well and the recovery of both forms was higher than 90% in plasma. A variety of structurally related proteins, including t-PA, were tested, but no reaction with proteins other than u-PA and its amino-terminal degradation product were observed. The intra-assay and inter-assay coefficients of variation for determination of u-PA in plasma were 7.6% and 8.4%, respectively. The assay was equally applicable to serum. The values obtained with plasma and serum were similar, and the results were not affected by small variations in the preparation of the samples. The ELISA was used to measure the concentration of u-PA in plasma from 34 healthy donors. The mean values for u-PA in plasma from healthy donors was 1.1 ng/ml ± 0.3 ng/ml (SD) (range 0.6 - 1.5 ng/ml). No significant differences were found between men and women and no correlation between u-PA concentration and age could be demonstrated.The mean u-PA concentration in plasma from healthy donors obtained in this study is substantially lower than that reported by others. This might be due to different methods of determination of the protein content of the standard preparations or to differences in the specificity of the assays.

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Effects of Macronutrients in the Physiological Quality of Soybean Seeds

Journal of Agricultural Science ◽

10.5539/jas.v10n5p312 ◽

2018 ◽

Vol 10 (5) ◽

pp. 312

Author(s):

Geliandro Anhaia Rigo ◽

Luis Osmar Braga Schuch ◽

Willian Silva Barros ◽

Rodrigo Lamaison de Vargas ◽

Vinícius Jardel Szareski ◽

...

Keyword(s):

Graduate Program ◽

Plant Tissues ◽

Soybean Seeds ◽

Rio Grande Do Sul ◽

Genetic Characteristics ◽

Physiological Quality ◽

High Concentrations ◽

Nitrogen Phosphorus

The aim of this work was to correlate macronutrient content of soybean seeds to physiological quality of different cultivars. The work was developed in the Federal University of Pelotas, in the facilities of the Seed Science and Technology Graduate Program. The experimental design was randomized blocks in arranged in four replicates. The following soybean cultivars were used: BMX Apolo RR (12 lots), BMX Ativa RR (13 lots), BMX Energia RR (26 lots), BMX Força RR (24 lots), BMX Impacto RR (35 lots), BMX Magna RR (16 lots), BMX Turbo RR (44 lots), BMX Potência RR (82 lots) and NA 5909 RR (28 lots), with seeds produced in northwest Rio Grande do Sul. The determination of nutritional contents found in plant tissues of soybean seeds, were measured: Nitrogen (N), Phosphorus (P), Potassium (K), Magnesium (Mg), Calcium (Ca), Sulfur (S). The macronutrient contents of the seeds vary according to genetic characteristics of the cultivars, with higher oscillations of nitrogen, phosphorus, magnesium and sulfur contents present in soybean seeds. Potassium and calcium are defined as the most stable nutrients for the cultivars and seed lots analyzed. High concentrations of nitrogen, phosphorus and calcium are determinants for the physiological quality of soybean seeds.

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AB0101 THE IMPACT OF TIME SPAN TO ACHIEVE BOOLEAN REMISSION FOR MAINTAINING DISEASE ACTIVITY AFTER ACQUISITION IN RHEUMATOID ARTHRITIS PATIENT

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2021-eular.297 ◽

2021 ◽

Vol 80 (Suppl 1) ◽

pp. 1079.1-1079

Author(s):

I. Yoshii

Keyword(s):

Rheumatoid Arthritis ◽

Disease Activity ◽

Disease Duration ◽

Remission Rate ◽

Mean Value ◽

Time Span ◽

Mean Values ◽

The Mean ◽

The Relationship

Background:Boolean remission criteria is one most popular and stringent criteria in treating patient with rheumatoid arthritis (RA), because it may guarantees a stable clinical course after attaining remission.Objectives:Impact of time span from initiation to achieving Boolean remission on maintaining disease activity, daily activities, and quality of life after attaining Boolean remission was investigated from daily clinical practice data.Methods:685 patients with RA since August 2010 under the T2T strategy were treated. They were monitored for their TJC, SJC, PGA, EGA, CRP, and disease activity indices such as CDAI, SDAI, DAS28, and Boolean criteria at every visit. HAQ-DI score, pain score using visual analog scale (PS-VAS), and EQ-5D were also monitored, and the quality of life score (QOLS) calculated from EQ-5D was determined at every visit from the time of diagnosis (baseline).Of 685 patients, 465 patients had achieved Boolean remission >1 times, and were consecutively followed up for >3 years. These patients were enrolled in the study. Time span from the first visit to first Boolean remission was calculated. The relationship between the time span and each of background parameters, and the relationship between the time span and each of the mean values of the SDAI score, HAQ score, PS-VAS, SHS, and QOLS at the first Boolean remission and thereafter was evaluated statistically.Patients were subsequently divided into the G ≤ 6 and G > 6 groups based on the achievement of first Boolean remission within two groups: time span G ≤ 6 months and G > 6 months. The two groups were compared with regard to the SDAI score, HAQ score, PS-VAS, SHS, and QOLS at first visit and at the time of first Boolean remission, and the mean values of these parameters after remission were evaluated statistically. Moreover, changes of these parameters and the mean Boolean remission rate after the first remission, and SDAI remission rate at the first Boolean remission to thereafter were compared between the two groups statistically.Results:Out of 465 patients, females comprised 343 (73.7%), and the mean age was 67.8 years (range, from 21–95 years). The mean disease duration at first visit was 6.1 years (range, from 1 months–45 years). The mean follow up length was 88.1 months (range: 36–122 months; median: 85 months) and mean time span from the first visit to the first Boolean remission was 8.1 months. The mean SDAI score, HAQ score, PS-VAS, and the QOLS at first visit were 13.3, 0.467, 33.2, and 0.834, respectively. Among the study parameters, PS-VAS and QOLS were significantly correlated with the time span. For parameters at the first Boolean remission, HAQ-DI score, PS-VAS, and QOLS demonstrated significant correlation with the time span, whereas SDAI, HAQ-DI score, PS-VAS, SHS, and QOLS after the Boolean remission demonstrated significant correlation with the time span.The comparison between the G ≤ 6 and the G > 6 groups revealed that the disease duration, HAQ score, and PS-VAS at baseline in the G > 6 were significantly higher than that in the G ≤ 6 group, and QOLS in the G ≤ 6 group was significantly higher than that in the G > 6 group at baseline. Similarly, the HAQ score and PS-VAS at the first Boolean remission in the G > 6 group were significantly higher than that in the G ≤ 6 group, whereas QOLS in the G ≤ 6 group demonstrated no significant difference compared with that in the G > 6 group.The mean value of the SDAI score after the first Boolean remission in the G > 6 group was significantly higher than that in the G ≤ 6 group. Similarly, the SDAI score, HAQ score, and PS-VAS after the first Boolean remission in the G > 6 group were also significantly higher than those in the G ≤ 6 group, and the mean value of the QOLS in the G ≤ 6 group were significantly higher than that in the G > 6 group. The Boolean remission rate and SDAI remission rate after the first Boolean remission were significantly higher in the G ≤ 6 group than those in the G > 6 group.Conclusion:Attaining Boolean remission ≤ 6 months for RA has significant benefit for more stable disease control, that leads good maintenance of ADL.Disclosure of Interests:None declared

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