Improved radioimmunoassay of melatonin in serum.
Keyword(s):
Abstract Melatonin was extracted from serum by using Baker reversed-phase C-18 columns. More than 99% of the applied melatonin was retained by the columns, and more than 97% was eluted from the columns in 300 microL of methanol. We then determined melatonin in the serum extract by a modification of a standard radioimmunoassay, using filtration instead of centrifugation to collect the [3H]melatonin-antibody complex precipitated by saturated ammonium sulfate. These modifications allow more rapid, accurate, and reproducible determination of melatonin than do previously published procedures.
2005 ◽
Vol 18
(106)
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pp. 450-454
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1983 ◽
Vol 43
(6)
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pp. 463-472
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2010 ◽
Vol 30
(2)
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pp. 193-195
2017 ◽
Vol 35
(10)
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pp. 1042
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