Negative Interference by Ethamsylate In Enzymatic Assay of Serum Creatinine Involving Peroxidase-Coupled Reaction

1992 ◽  
Vol 38 (1) ◽  
pp. 169-170 ◽  
Author(s):  
Ryo Fushimi ◽  
Akemi Suminoe ◽  
Masayoshi Yasuhara ◽  
Etsuji Suehisa ◽  
Masahiko Matsui ◽  
...  
Keyword(s):  
Serum Creatinine ◽  
Enzymatic Assay ◽  
Negative Interference ◽  
Coupled Reaction

scholarly journals Strong Negative Interference by Calcium Dobesilate in Sarcosine Oxidase Assays for Serum Creatinine Involving the Trinder Reaction

Medicine ◽  
2015 ◽  
Vol 94 (23) ◽  
pp. e905 ◽  
Author(s):  
Xiuzhi Guo ◽  
Li’an Hou ◽  
Xinqi Cheng ◽  
Tianjiao Zhang ◽  
Songlin Yu ◽  
...  
Keyword(s):  
Serum Creatinine ◽  
Calcium Dobesilate ◽  
Negative Interference ◽  
Sarcosine Oxidase

Use of a synthetic soluble bilirubin derivative to assess interference in creatinine measurements

1991 ◽  
Vol 37 (2) ◽  
pp. 236-238 ◽  
Author(s):  
Carlo Franzini ◽  
Anna M Morelli ◽  
Glanpaolo Cattozzo
Keyword(s):  
Hydrogen Peroxide ◽  
Serum Creatinine ◽  
Interference Effect ◽  
Nadh Oxidation ◽  
Enzymatic Method ◽  
Negative Interference ◽  
Significant Interference ◽  
Creatinine Measurement ◽  
Serum Creatinine Measurement ◽  
Enzymatic Methods

Abstract In assessing interference from bilirubin, the use of a synthetic soluble derivative (ditaurobilirubin, DTB) is recommended as a surrogate for the natural conjugates (Bc). We compared the interference effect of unconjugated bilirubin (Bu), Bc, and DTB, using six mechanized methods for serum creatinine measurement. No significant interference was noted in methods that include removal of proteins or in an enzymatic method involving NADH oxidation. Heavy (negative) interference was observed in an alkaline picrate method, and in direct enzymatic methods based on hydrogen peroxide measurement: interference was always more pronounced in the presence of the two soluble derivatives (Bc and DTB), whose interference was of the same magnitude. These results point out the utility of testing for bilirubin interference by using soluble derivatives, in addition to Bu, and suggest the feasibility of using DTB as a surrogate for Bc for this purpose.

Lactulose interferes in the alkaline picrate assay for creatinine.

1988 ◽  
Vol 34 (12) ◽  
pp. 2592-2593 ◽  
Author(s):  
D E Bruns
Keyword(s):  
Serum Creatinine ◽  
Continuous Flow ◽  
Linear Increase ◽  
Enzymatic Assay ◽  
Automated Methods

Abstract Errors of more than 10 mg/L in measured serum creatinine concentrations were encountered for an azotemic patient who was given lactulose orally. The magnitude of the apparent error fluctuated with the dose of lactulose. Additions of lactulose to serum produced a linear increase in the creatinine measured by each of two automated methods that involve use of the alkaline picrate (Jaffé) reaction. A lactulose concentration of 100 g/L produced positive interferences of 30 and 65 mg/L in kinetic (Beckman Astra) and continuous-flow (Technicon SMAC) assays, respectively, but caused no problem in an enzymatic assay for creatinine. The results of creatinine assays must be interpreted with caution in patients treated with lactulose.

scholarly journals Variable Creatinine Levels in Critical Care Patients: A Concerning Knowledge Gap

2021 ◽  
Vol 10 (8) ◽  
pp. 1689
Author(s):  
Trushil Shah ◽  
Madhusudhanan Narasimhan ◽  
Mary Latha Rathinam ◽  
Karen Relle ◽  
Melanie Kim ◽  
...  
Keyword(s):  
Critical Care ◽  
Serum Creatinine ◽  
Clinical Laboratory ◽  
Test Accuracy ◽  
Longitudinal Assessment ◽  
Cross Sectional ◽  
Negative Interference ◽  
Survey Results ◽  
Picc Line ◽  
Critical Care Patients

An accurate creatinine (Cr) estimate is pivotal for the assessment of renal function. Both patient- and practice-spawned factors palliate the test accuracy of serum creatinine (sCr) and can erratically represent actual kidney function. This study evaluated the caregivers’ awareness of enzymatic serum creatinine (E-sCr) assay interfering in dopamine/dobutamine (DD)-infused patient samples and the frequency of such interference in a critical care setting. We conducted an sCr awareness survey among UT Southwestern physicians, nurses, and pharmacists. We then performed a cross-sectional E-sCr comparison against the kinetic Jaffe method using the DD-infused patient samples collected from central venous catheters (CVC), peripherally inserted central catheter (PICC) lines, and the peripheral vein (PV). We retrospectively compared the longitudinal E-sCr results of the CVC/PICC draws with the corresponding blood urea nitrogen (BUN) levels. The survey results show a significant lack of awareness among caregivers about the negative interference of DD infusions on E-sCr. Cross-sectional E-sCr assessment relative to the Jaffe method displayed a negative interference in 12% of CVC/PICC line samples (7/57 DD-infused patients) compared to none in the PV draws. A longitudinal assessment of E-sCr, BUN, and potassium (K) levels from CVC/PICC line samples further confirmed a spurious decrease for E-sCr in about 12/50 (24%) patients who did not show a concurrent BUN or K decrease. The results suggest that a direct PV sampling accompanied by clinical laboratory-directed proactive discussion/activities can foster awareness among caregivers and eschew the false E-sCr estimates in DD-infused patients.

Assessment of Glomerular Filtration Rate in Preterm Infants by Serum Creatinine: Comparison With Inulin Clearance

PEDIATRICS ◽  
1995 ◽  
Vol 96 (6) ◽  
pp. 1156-1158
Author(s):  
John N. van den Anker ◽  
Ronald de Groot ◽  
Henriette M. Broerse ◽  
Pieter J. J. Sauer ◽  
Bert J. van der Heijden ◽  
...  
Keyword(s):  
Glomerular Filtration Rate ◽  
Serum Creatinine ◽  
Glomerular Filtration ◽  
Preterm Infants ◽  
Filtration Rate ◽  
Clinical Chemistry ◽  
Fluid Management ◽  
Chemistry Laboratory ◽  
Negative Interference ◽  
The Individual

Dosage regimens of drugs that are cleared mainly by glomerular filtration as well as fluid management in preterm infants should be based on the glomerular filtration rate (GFR) of the individual patient. However, GFR measurements and collection of urine in newborns are difficult to perform. The 24 to 48 h continuous inulin infusion technique does not require the collection of urine and is considered the most reliable indicator of GFR.1,2 This method is invasive, time-consuming, and expensive. In contrast, serum creatinine measurements can be obtained easily and determined quickly in the clinical chemistry laboratory. Most laboratories use an automated kinetic Jaffé method, which is subject to negative interference by plasma hemoglobin above 0.06 mmol/L, and to negative interference by bilirubin (about 35 µmol/L by a serum bilirubin of about 100 µmol/L).

Falsely low estimation of triglycerides in lipemic plasma by the enzymatic triglyceride method with modified Trinder's chromogen

1990 ◽  
Vol 36 (2) ◽  
pp. 325-329 ◽  
Author(s):  
M D Shephard ◽  
M J Whiting
Keyword(s):  
Electron Acceptor ◽  
Marked Decrease ◽  
Linear Range ◽  
Enzymatic Assay ◽  
Negative Interference

Abstract The enzymatic assay of triglyceride, based on the use of L-glycerol-3-phosphate oxidase (EC 1.1.3.21) and a modified Trinder's chromogen involving 4-chlorophenol, is subject to strong negative interference at concentrations of triglyceride greater than 20 mmol/L, such as occur in grossly lipemic plasma. This interference is caused by the rapid utilization of oxygen, resulting in the reaction becoming transiently anaerobic. The dye product already formed may then be reduced ("bleached") by acting as an alternative electron acceptor for glycerol-3-phosphate oxidase. Reduction of the dye leads to a marked decrease in final absorbance at 505 nm. Grossly underestimated values for triglyceride concentrations, apparently within the linear range of the assay, may therefore be inadvertently obtained with equilibrium methods. We suggest that samples giving unexpectedly low results for lipemic plasma should be re-assayed after dilution or with use of a smaller volume of sample.

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