scholarly journals Strong Negative Interference by Calcium Dobesilate in Sarcosine Oxidase Assays for Serum Creatinine Involving the Trinder Reaction

Medicine ◽  
2015 ◽  
Vol 94 (23) ◽  
pp. e905 ◽  
Author(s):  
Xiuzhi Guo ◽  
Li’an Hou ◽  
Xinqi Cheng ◽  
Tianjiao Zhang ◽  
Songlin Yu ◽  
...  
Keyword(s):  
Serum Creatinine ◽  
Calcium Dobesilate ◽  
Negative Interference ◽  
Sarcosine Oxidase

Definitive liquid-chromatographic demonstration that N-ethylglycine is the metabolite of lidocaine that interferes in the Kodak sarcosine oxidase-coupled method for creatinine.

1988 ◽  
Vol 34 (12) ◽  
pp. 2569-2572 ◽  
Author(s):  
R T Roberts ◽  
N M Alexander ◽  
M J Kelner
Keyword(s):  
Serum Creatinine ◽  
Human Serum ◽  
Sarcosine Oxidase ◽  
Coupled Method ◽  
Linear Relationships ◽  
Liquid Chromatographic ◽  
Drug Free ◽  
Single Slide

Abstract Patients receiving lidocaine may show false increases of serum creatinine as assayed by the single-slide method on the Kodak Ektachem 700. Bissell et al. (Clin Chem 1987;33:951) suggested that this interference was due to oxidation of N-ethylglycine (NEG), a previously uncharacterized metabolite of lidocaine, by the sarcosine oxidase preparation used in the Ektachem creatinine slide. To investigate this possibility, we synthesized NEG, added it to drug-free human serum, and analyzed the NEG-supplemented sera for creatinine with the Ektachem 700. We found the following linear relationships between creatinine bias (y, mg/L) and NEG concentration (x, mg/L) for first (I), third (III), and fourth (IV) generation slides: I: y = 1.70x - 0.8 mg/L (n = 13, r = 1.0) III: y = 0.39x - 0.3 mg/L (n = 3, r = 1.0) IV: y = 0.79x - 1.8 mg/L (n = 13, r = 1.0) Using HPLC, we directly demonstrated the presence of NEG in sera of patients receiving lidocaine and quantified NEG concentrations in sera from four of these patients. The increasing artifactual bias in creatinine with increasing NEG concentration unequivocally confirmed that NEG is responsible for the lidocaine-associated interference in the Kodak Ektachem single-slide creatinine method.

Use of a synthetic soluble bilirubin derivative to assess interference in creatinine measurements

1991 ◽  
Vol 37 (2) ◽  
pp. 236-238 ◽  
Author(s):  
Carlo Franzini ◽  
Anna M Morelli ◽  
Glanpaolo Cattozzo
Keyword(s):  
Hydrogen Peroxide ◽  
Serum Creatinine ◽  
Interference Effect ◽  
Nadh Oxidation ◽  
Enzymatic Method ◽  
Negative Interference ◽  
Significant Interference ◽  
Creatinine Measurement ◽  
Serum Creatinine Measurement ◽  
Enzymatic Methods

Abstract In assessing interference from bilirubin, the use of a synthetic soluble derivative (ditaurobilirubin, DTB) is recommended as a surrogate for the natural conjugates (Bc). We compared the interference effect of unconjugated bilirubin (Bu), Bc, and DTB, using six mechanized methods for serum creatinine measurement. No significant interference was noted in methods that include removal of proteins or in an enzymatic method involving NADH oxidation. Heavy (negative) interference was observed in an alkaline picrate method, and in direct enzymatic methods based on hydrogen peroxide measurement: interference was always more pronounced in the presence of the two soluble derivatives (Bc and DTB), whose interference was of the same magnitude. These results point out the utility of testing for bilirubin interference by using soluble derivatives, in addition to Bu, and suggest the feasibility of using DTB as a surrogate for Bc for this purpose.

scholarly journals Variable Creatinine Levels in Critical Care Patients: A Concerning Knowledge Gap

2021 ◽  
Vol 10 (8) ◽  
pp. 1689
Author(s):  
Trushil Shah ◽  
Madhusudhanan Narasimhan ◽  
Mary Latha Rathinam ◽  
Karen Relle ◽  
Melanie Kim ◽  
...  
Keyword(s):  
Critical Care ◽  
Serum Creatinine ◽  
Clinical Laboratory ◽  
Test Accuracy ◽  
Longitudinal Assessment ◽  
Cross Sectional ◽  
Negative Interference ◽  
Survey Results ◽  
Picc Line ◽  
Critical Care Patients

An accurate creatinine (Cr) estimate is pivotal for the assessment of renal function. Both patient- and practice-spawned factors palliate the test accuracy of serum creatinine (sCr) and can erratically represent actual kidney function. This study evaluated the caregivers’ awareness of enzymatic serum creatinine (E-sCr) assay interfering in dopamine/dobutamine (DD)-infused patient samples and the frequency of such interference in a critical care setting. We conducted an sCr awareness survey among UT Southwestern physicians, nurses, and pharmacists. We then performed a cross-sectional E-sCr comparison against the kinetic Jaffe method using the DD-infused patient samples collected from central venous catheters (CVC), peripherally inserted central catheter (PICC) lines, and the peripheral vein (PV). We retrospectively compared the longitudinal E-sCr results of the CVC/PICC draws with the corresponding blood urea nitrogen (BUN) levels. The survey results show a significant lack of awareness among caregivers about the negative interference of DD infusions on E-sCr. Cross-sectional E-sCr assessment relative to the Jaffe method displayed a negative interference in 12% of CVC/PICC line samples (7/57 DD-infused patients) compared to none in the PV draws. A longitudinal assessment of E-sCr, BUN, and potassium (K) levels from CVC/PICC line samples further confirmed a spurious decrease for E-sCr in about 12/50 (24%) patients who did not show a concurrent BUN or K decrease. The results suggest that a direct PV sampling accompanied by clinical laboratory-directed proactive discussion/activities can foster awareness among caregivers and eschew the false E-sCr estimates in DD-infused patients.

Negative Interference by Ethamsylate In Enzymatic Assay of Serum Creatinine Involving Peroxidase-Coupled Reaction

1992 ◽  
Vol 38 (1) ◽  
pp. 169-170 ◽  
Author(s):  
Ryo Fushimi ◽  
Akemi Suminoe ◽  
Masayoshi Yasuhara ◽  
Etsuji Suehisa ◽  
Masahiko Matsui ◽  
...  
Keyword(s):  
Serum Creatinine ◽  
Enzymatic Assay ◽  
Negative Interference ◽  
Coupled Reaction

Assessment of Glomerular Filtration Rate in Preterm Infants by Serum Creatinine: Comparison With Inulin Clearance

PEDIATRICS ◽  
1995 ◽  
Vol 96 (6) ◽  
pp. 1156-1158
Author(s):  
John N. van den Anker ◽  
Ronald de Groot ◽  
Henriette M. Broerse ◽  
Pieter J. J. Sauer ◽  
Bert J. van der Heijden ◽  
...  
Keyword(s):  
Glomerular Filtration Rate ◽  
Serum Creatinine ◽  
Glomerular Filtration ◽  
Preterm Infants ◽  
Filtration Rate ◽  
Clinical Chemistry ◽  
Fluid Management ◽  
Chemistry Laboratory ◽  
Negative Interference ◽  
The Individual

Dosage regimens of drugs that are cleared mainly by glomerular filtration as well as fluid management in preterm infants should be based on the glomerular filtration rate (GFR) of the individual patient. However, GFR measurements and collection of urine in newborns are difficult to perform. The 24 to 48 h continuous inulin infusion technique does not require the collection of urine and is considered the most reliable indicator of GFR.1,2 This method is invasive, time-consuming, and expensive. In contrast, serum creatinine measurements can be obtained easily and determined quickly in the clinical chemistry laboratory. Most laboratories use an automated kinetic Jaffé method, which is subject to negative interference by plasma hemoglobin above 0.06 mmol/L, and to negative interference by bilirubin (about 35 µmol/L by a serum bilirubin of about 100 µmol/L).

Sign in / Sign up

Export Citation Format

Share Document