scholarly journals A new method for anti‐negative interference of calcium dobesilate in serum creatinine enzymatic analysis

2021 ◽  
Author(s):  
Hailan Shen ◽  
Kena Chen ◽  
Ju Cao
Keyword(s):  
Serum Creatinine ◽  
New Method ◽  
Enzymatic Analysis ◽  
Calcium Dobesilate ◽  
Negative Interference

scholarly journals Strong Negative Interference by Calcium Dobesilate in Sarcosine Oxidase Assays for Serum Creatinine Involving the Trinder Reaction

Medicine ◽  
2015 ◽  
Vol 94 (23) ◽  
pp. e905 ◽  
Author(s):  
Xiuzhi Guo ◽  
Li’an Hou ◽  
Xinqi Cheng ◽  
Tianjiao Zhang ◽  
Songlin Yu ◽  
...  
Keyword(s):  
Serum Creatinine ◽  
Calcium Dobesilate ◽  
Negative Interference ◽  
Sarcosine Oxidase

New Method for Direct Determination of "True" Creatinine

1974 ◽  
Vol 20 (9) ◽  
pp. 1131-1134 ◽  
Author(s):  
Hippocrates Yatzidis
Keyword(s):  
Ion Exchange ◽  
Serum Creatinine ◽  
Direct Determination ◽  
Direct Analysis ◽  
Color Reaction ◽  
New Method ◽  
Exchange Method ◽  
Ion Exchange Method ◽  
Serum And Urine

Abstract Determination of serum creatinine by the Jaffé reaction may give erroneous results because of interfering noncreatinine chromogens. Studies of the Jaffé reaction in alkaline picrate media of various pH demonstrated that creatinine, proteins, and certain chromogens have characteristic properties as regards the appearance and intensity of the color reaction. Thus a method for determining "true" creatinine has been developed in which simple spectrophotometric measurements are made at 500 nm in two alkaline picrate reagents, buffered at pH 9.65 and 11.50. Results agree well with those obtained by the use of an ion-exchange method. The proposed method is easy and rapid and offers the advantages of increased specificity and direct analysis of serum and urine.

Use of a synthetic soluble bilirubin derivative to assess interference in creatinine measurements

1991 ◽  
Vol 37 (2) ◽  
pp. 236-238 ◽  
Author(s):  
Carlo Franzini ◽  
Anna M Morelli ◽  
Glanpaolo Cattozzo
Keyword(s):  
Hydrogen Peroxide ◽  
Serum Creatinine ◽  
Interference Effect ◽  
Nadh Oxidation ◽  
Enzymatic Method ◽  
Negative Interference ◽  
Significant Interference ◽  
Creatinine Measurement ◽  
Serum Creatinine Measurement ◽  
Enzymatic Methods

Abstract In assessing interference from bilirubin, the use of a synthetic soluble derivative (ditaurobilirubin, DTB) is recommended as a surrogate for the natural conjugates (Bc). We compared the interference effect of unconjugated bilirubin (Bu), Bc, and DTB, using six mechanized methods for serum creatinine measurement. No significant interference was noted in methods that include removal of proteins or in an enzymatic method involving NADH oxidation. Heavy (negative) interference was observed in an alkaline picrate method, and in direct enzymatic methods based on hydrogen peroxide measurement: interference was always more pronounced in the presence of the two soluble derivatives (Bc and DTB), whose interference was of the same magnitude. These results point out the utility of testing for bilirubin interference by using soluble derivatives, in addition to Bu, and suggest the feasibility of using DTB as a surrogate for Bc for this purpose.

scholarly journals Variable Creatinine Levels in Critical Care Patients: A Concerning Knowledge Gap

2021 ◽  
Vol 10 (8) ◽  
pp. 1689
Author(s):  
Trushil Shah ◽  
Madhusudhanan Narasimhan ◽  
Mary Latha Rathinam ◽  
Karen Relle ◽  
Melanie Kim ◽  
...  
Keyword(s):  
Critical Care ◽  
Serum Creatinine ◽  
Clinical Laboratory ◽  
Test Accuracy ◽  
Longitudinal Assessment ◽  
Cross Sectional ◽  
Negative Interference ◽  
Survey Results ◽  
Picc Line ◽  
Critical Care Patients

An accurate creatinine (Cr) estimate is pivotal for the assessment of renal function. Both patient- and practice-spawned factors palliate the test accuracy of serum creatinine (sCr) and can erratically represent actual kidney function. This study evaluated the caregivers’ awareness of enzymatic serum creatinine (E-sCr) assay interfering in dopamine/dobutamine (DD)-infused patient samples and the frequency of such interference in a critical care setting. We conducted an sCr awareness survey among UT Southwestern physicians, nurses, and pharmacists. We then performed a cross-sectional E-sCr comparison against the kinetic Jaffe method using the DD-infused patient samples collected from central venous catheters (CVC), peripherally inserted central catheter (PICC) lines, and the peripheral vein (PV). We retrospectively compared the longitudinal E-sCr results of the CVC/PICC draws with the corresponding blood urea nitrogen (BUN) levels. The survey results show a significant lack of awareness among caregivers about the negative interference of DD infusions on E-sCr. Cross-sectional E-sCr assessment relative to the Jaffe method displayed a negative interference in 12% of CVC/PICC line samples (7/57 DD-infused patients) compared to none in the PV draws. A longitudinal assessment of E-sCr, BUN, and potassium (K) levels from CVC/PICC line samples further confirmed a spurious decrease for E-sCr in about 12/50 (24%) patients who did not show a concurrent BUN or K decrease. The results suggest that a direct PV sampling accompanied by clinical laboratory-directed proactive discussion/activities can foster awareness among caregivers and eschew the false E-sCr estimates in DD-infused patients.

Negative Interference by Ethamsylate In Enzymatic Assay of Serum Creatinine Involving Peroxidase-Coupled Reaction

1992 ◽  
Vol 38 (1) ◽  
pp. 169-170 ◽  
Author(s):  
Ryo Fushimi ◽  
Akemi Suminoe ◽  
Masayoshi Yasuhara ◽  
Etsuji Suehisa ◽  
Masahiko Matsui ◽  
...  
Keyword(s):  
Serum Creatinine ◽  
Enzymatic Assay ◽  
Negative Interference ◽  
Coupled Reaction

Assessment of Glomerular Filtration Rate in Preterm Infants by Serum Creatinine: Comparison With Inulin Clearance

PEDIATRICS ◽  
1995 ◽  
Vol 96 (6) ◽  
pp. 1156-1158
Author(s):  
John N. van den Anker ◽  
Ronald de Groot ◽  
Henriette M. Broerse ◽  
Pieter J. J. Sauer ◽  
Bert J. van der Heijden ◽  
...  
Keyword(s):  
Glomerular Filtration Rate ◽  
Serum Creatinine ◽  
Glomerular Filtration ◽  
Preterm Infants ◽  
Filtration Rate ◽  
Clinical Chemistry ◽  
Fluid Management ◽  
Chemistry Laboratory ◽  
Negative Interference ◽  
The Individual

Dosage regimens of drugs that are cleared mainly by glomerular filtration as well as fluid management in preterm infants should be based on the glomerular filtration rate (GFR) of the individual patient. However, GFR measurements and collection of urine in newborns are difficult to perform. The 24 to 48 h continuous inulin infusion technique does not require the collection of urine and is considered the most reliable indicator of GFR.1,2 This method is invasive, time-consuming, and expensive. In contrast, serum creatinine measurements can be obtained easily and determined quickly in the clinical chemistry laboratory. Most laboratories use an automated kinetic Jaffé method, which is subject to negative interference by plasma hemoglobin above 0.06 mmol/L, and to negative interference by bilirubin (about 35 µmol/L by a serum bilirubin of about 100 µmol/L).

Effects of calcium dobesilate (CaD) interference on serum creatinine measurements: a national External Quality Assessment (EQA)-based educational survey of drug-laboratory test interactions

2021 ◽  
Vol 59 (1) ◽  
pp. 139-145
Author(s):  
Tianjiao Zhang ◽  
Xiuzhi Guo ◽  
Li’an Hou ◽  
Haijian Zhao ◽  
Rong Ma ◽  
...  
Keyword(s):  
Serum Creatinine ◽  
Laboratory Test ◽  
Laboratory Data ◽  
Negative Influence ◽  
Current Status ◽  
Data Systems ◽  
Enzymatic Method ◽  
Calcium Dobesilate ◽  
Laboratory Errors ◽  
Clinical Laboratories

AbstractObjectivesDrug-laboratory test interactions (DLTIs) are one of the major sources of laboratory errors. Calcium dobesilate (CaD) interference on serum creatinine testing is a widespread problem that has long been ignored in China. A national EQA-based survey was launched to investigate the current status of CaD interference on creatinine routine methods used in China and enhance the education of CaD interference in clinical laboratories.MethodsA descriptive survey was developed to characterize the status quo of Chinese laboratory professionals’ cognition to CaD interference. Four of survey samples which were spiked with/without interference additive were shipped to 175 participant laboratories. The target reference values from a reference measurement procedure were compared against the results from participating laboratories to evaluate the CaD interference on serum creatinine measurements using enzymatic method or Jaffé method.ResultsThe lack of knowledge of DLTIs and the barriers to collect information from pharmacological and laboratory data systems had become the main problems on implementing DLTIs education in China. A significant negative influence of CaD on enzymatic method was observed regardless of measurement platforms. Jaffé method was generally free from interaction with CaD but showed poor precision and accuracy at low creatinine concentrations.ConclusionsMore efforts should be made to enhance the education of DLTIs in clinical laboratories in China.

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