scholarly journals The novel DNA damage checkpoint protein Ddc1p is phosphorylated periodically during the cell cycle and in response to DNA damage in budding yeast

1997 ◽  
Vol 16 (17) ◽  
pp. 5216-5226 ◽  
Author(s):  
M. P. Longhese
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Budding Yeast ◽  
Dna Damage Checkpoint ◽  
The Novel ◽  
Checkpoint Protein

scholarly journals The DNA damage checkpoint protein ATM promotes hepatocellular apoptosis and fibrosis in a mouse model of non-alcoholic fatty liver disease

Cell Cycle ◽  
2012 ◽  
Vol 11 (10) ◽  
pp. 1918-1928 ◽  
Author(s):  
Erin K. Daugherity ◽  
Gabriel Balmus ◽  
Ahmed Al Saei ◽  
Elizabeth S. Moore ◽  
Delbert Abi Abdallah ◽  
...  
Keyword(s):  
Dna Damage ◽  
Liver Disease ◽  
Mouse Model ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Dna Damage Checkpoint ◽  
Alcoholic Fatty Liver ◽  
Checkpoint Protein ◽  
Hepatocellular Apoptosis ◽  
Alcoholic Fatty Liver Disease

CELL CYCLE: Enhanced: A DNA Damage Checkpoint Meets the Cell Cycle Engine

Science ◽  
1997 ◽  
Vol 277 (5331) ◽  
pp. 1450-1451 ◽  
Author(s):  
T. Weinert
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Dna Damage Checkpoint

Budding Yeast DNA Damage Checkpoint: A Signal Transduction-Mediated Surveillance System

2003 ◽  
pp. 197-202
Author(s):  
Marco Muzi-Falconi ◽  
Michele Giannattasio ◽  
Giordano Liberi ◽  
Achille Pelliccioli ◽  
Paolo Plevani ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Dna Damage ◽  
Surveillance System ◽  
Budding Yeast ◽  
Dna Damage Checkpoint

Details and Concerns Regarding the G2/M DNA Damage Checkpoint in Budding Yeast

2000 ◽  
Vol 65 (0) ◽  
pp. 433-442 ◽  
Author(s):  
T. WEINERT ◽  
E. LITTLE ◽  
L. SHANKS ◽  
A. ADMIRE ◽  
R. GARDNER ◽  
...  
Keyword(s):  
Dna Damage ◽  
Budding Yeast ◽  
Dna Damage Checkpoint

TORC1 regulates the DNA damage checkpoint via checkpoint protein levels

2019 ◽  
Vol 510 (4) ◽  
pp. 629-635 ◽  
Author(s):  
Ikuko Miyamoto ◽  
Ryota Ozaki ◽  
Kazuyuki Yamaguchi ◽  
Kaori Yamamoto ◽  
Atsuki Kaneko ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Damage Checkpoint ◽  
Checkpoint Protein ◽  
Protein Levels

scholarly journals Direct and Indirect Control of the Initiation of Meiotic Recombination by DNA Damage Checkpoint Mechanisms in Budding Yeast

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e65875 ◽  
Author(s):  
Bilge Argunhan ◽  
Sarah Farmer ◽  
Wing-Kit Leung ◽  
Yaroslav Terentyev ◽  
Neil Humphryes ◽  
...  
Keyword(s):  
Dna Damage ◽  
Meiotic Recombination ◽  
Budding Yeast ◽  
Dna Damage Checkpoint ◽  
Indirect Control

scholarly journals Recruitment of DNA Damage Checkpoint Proteins to Damage in Transcribed and Nontranscribed Sequences

2006 ◽  
Vol 26 (1) ◽  
pp. 39-49 ◽  
Author(s):  
Guochun Jiang ◽  
Aziz Sancar
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Excision Repair ◽  
Dna Lesions ◽  
Human Cells ◽  
Dna Damage Checkpoint ◽  
Damage Site ◽  
Checkpoint Response ◽  
Checkpoint Proteins ◽  
Transcription Complexes

ABSTRACT We developed a chromatin immunoprecipitation method for analyzing the binding of repair and checkpoint proteins to DNA base lesions in any region of the human genome. Using this method, we investigated the recruitment of DNA damage checkpoint proteins RPA, Rad9, and ATR to base damage induced by UV and acetoxyacetylaminofluorene in transcribed and nontranscribed regions in wild-type and excision repair-deficient human cells in G1 and S phases of the cell cycle. We find that all 3 damage sensors tested assemble at the site or in the vicinity of damage in the absence of DNA replication or repair and that transcription enhances recruitment of checkpoint proteins to the damage site. Furthermore, we find that UV irradiation of human cells defective in excision repair leads to phosphorylation of Chk1 kinase in both G1 and S phase of the cell cycle, suggesting that primary DNA lesions as well as stalled transcription complexes may act as signals to initiate the DNA damage checkpoint response.

scholarly journals Phosphorylation of the Budding Yeast 9-1-1 Complex Is Required for Dpb11 Function in the Full Activation of the UV-Induced DNA Damage Checkpoint

2008 ◽  
Vol 28 (15) ◽  
pp. 4782-4793 ◽  
Author(s):  
Fabio Puddu ◽  
Magda Granata ◽  
Lisa Di Nola ◽  
Alessia Balestrini ◽  
Gabriele Piergiovanni ◽  
...  
Keyword(s):  
Dna Damage ◽  
Budding Yeast ◽  
Dna Damage Checkpoint ◽  
Checkpoint Activation ◽  
Checkpoint Response ◽  
Checkpoint Proteins ◽  
M Phase ◽  
Mutant Cells ◽  
Full Activation

ABSTRACT Following genotoxic insults, eukaryotic cells trigger a signal transduction cascade known as the DNA damage checkpoint response, which involves the loading onto DNA of an apical kinase and several downstream factors. Chromatin modifications play an important role in recruiting checkpoint proteins. In budding yeast, methylated H3-K79 is bound by the checkpoint factor Rad9. Loss of Dot1 prevents H3-K79 methylation, leading to a checkpoint defect in the G1 phase of the cell cycle and to a reduction of checkpoint activation in mitosis, suggesting that another pathway contributes to Rad9 recruitment in M phase. We found that the replication factor Dpb11 is the keystone of this second pathway. dot1Δ dpb11-1 mutant cells are sensitive to UV or Zeocin treatment and cannot activate Rad53 if irradiated in M phase. Our data suggest that Dpb11 is held in proximity to damaged DNA through an interaction with the phosphorylated 9-1-1 complex, leading to Mec1-dependent phosphorylation of Rad9. Dpb11 is also phosphorylated after DNA damage, and this modification is lost in a nonphosphorylatable ddc1-T602A mutant. Finally, we show that, in vivo, Dpb11 cooperates with Dot1 in promoting Rad9 phosphorylation but also contributes to the full activation of Mec1 kinase.

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