scholarly journals Frequency of viral nucleic acids detection using real-time PCR in endomyocardial biopsy samples, blood samples from the ventricle and peripheral blood in patients with dilated cardiomyopathy

2013 ◽  
Vol 34 (suppl 1) ◽  
pp. 2822-2822
Author(s):  
A. Pawlak ◽  
M. Przybylski ◽  
O. Mozenska ◽  
K. Gil ◽  
W. Grajkowska ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Dilated Cardiomyopathy ◽  
Real Time ◽  
Real Time Pcr ◽  
Peripheral Blood ◽  
Endomyocardial Biopsy ◽  
Blood Samples ◽  
Nucleic Acids Detection

scholarly journals Brucellosis laboratory tests in Syria: what are their diagnostic efficacies in different clinical manifestations?

2012 ◽  
Vol 6 (06) ◽  
pp. 495-500 ◽  
Author(s):  
Yara Alsayed ◽  
Fawza Monem
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Peripheral Blood ◽  
Laboratory Tests ◽  
Clinical Manifestations ◽  
High Sensitivity ◽  
Agglutination Test ◽  
Blood Samples ◽  
Culture Positive ◽  
Rule Out

Introduction: Diagnosis of brucellosis in Syria is based on the presence of compatible symptoms in addition to positive agglutination results. This study investigated the potential of culture, ELISA and real-time PCR to support the diagnosis in different clinical manifestations of brucellosis. Methodology: Peripheral blood samples from 34 suspected brucellosis patients and 42 probable chronic or relapsed brucellosis patients were tested by agglutination tests, culture, ELISA and real-time PCR. Results: Among 34 samples collected from suspected cases, 18/34 (53%) were agglutination tests positive, 12/34 (35%) were culture positive, 12/34 (35%) were Brucella IgG positive, and 10/34 (29%) were real-time PCR positive. Three out of 34 patients were positive by real-time PCR but not by agglutination tests or culture. Among 42 samples obtained from probable chronic or relapsed patients, 27/42 (64%) were agglutination tests positive, 26/42 (62%) were Brucella IgG positive, 4/42 (10%) were culture positive, and 1/42 (2%) was real-time PCR positive. Conclusion: To rule in or rule out the diagnosis of brucellosis, a combination of several tests should be applied. Agglutination tests should be performed first considering their high sensitivity. If the agglutination test is negative, real-time PCR, and/or ELISA, and/or culture should be performed. When relapse or chronic brucellosis are suspected, agglutination tests and/or ELISA are recommended.

Free circulating nucleic acids in plasma and serum as a novel approach to the use of internal controls in real time PCR based detection

2014 ◽  
Vol 207 ◽  
pp. 133-137 ◽  
Author(s):  
Ersin Karataylı ◽  
Yasemin Çelik Altunoğlu ◽  
Senem Ceren Karataylı ◽  
Cihan Yurdaydın ◽  
A. Mithat Bozdayı
Keyword(s):  
Nucleic Acids ◽  
Real Time ◽  
Real Time Pcr ◽  
Internal Controls ◽  
Circulating Nucleic Acids ◽  
Novel Approach

scholarly journals Detection of Aspergillus species in bone marrow transplant patients

2010 ◽  
Vol 4 (08) ◽  
pp. 511-516 ◽  
Author(s):  
Parisa Badiee ◽  
Abdolvahab Alborzi
Keyword(s):  
Bone Marrow ◽  
Real Time ◽  
Bone Marrow Transplant ◽  
Real Time Pcr ◽  
Clinical Signs ◽  
Marrow Transplant ◽  
Aspergillus Species ◽  
Pcr Assay ◽  
Blood Samples ◽  
Transplant Patients

Introduction:  Invasive aspergillosis is a severe complication of cytotoxic chemotherapies and bone marrow transplantation (BMT). The aim of this study was to assess the utility of a real-time PCR assay for the early diagnosis of Aspergillus species in blood samples from BMT patients. Methodology: Blood specimens (n = 993) from patients (n = 82) scheduled for BMT were collected prior to transplant and for 100 days post transplantation.  The specimens were later tested using an Aspergillus-specific real-time PCR assay. Cultures of clinical samples, along with sonography and computerized tomographic scans, were performed as standard of care. Results: Aspergillus DNA was positive in 94 sequential blood samples from 13 patients with clinical and radiological signs of infection. Samples from three of these patients were PCR-positive for Aspergillus in the first week of admission, prior to transplantation. Four patients with aspergillosis were cured with antifungal agents and nine died. An additional 12 patients without clinical signs of infection were PCR-positive on one occasion each, while two patients with clinical signs of infection were PCR-negative. Compared to routine methods of aspergillosis diagnosis, the respective sensitivity, specificity, negative, and positive predictive values of the PCR method by patient were 86.6%, 82%, 96.5% and 52%. Conclusions: The results show that Aspergillus infections in the blood of bone marrow transplant patients can be dectected by PCR methods. Early detection of Aspergillus infections by PCR has the potential to positively impact patient mortality rate and provide cost savings to hospitals.

scholarly journals Stability of hepatitis C virus RNA in blood samples by TaqMan real-time PCR

2010 ◽  
Vol 24 (3) ◽  
pp. 134-138 ◽  
Author(s):  
Kenan Sener ◽  
Mehmet Yapar ◽  
Orhan Bedir ◽  
Cem Gül ◽  
Ömer Coskun ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Real Time ◽  
Real Time Pcr ◽  
Hepatitis C Virus Rna ◽  
Blood Samples ◽  
Virus Rna
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