scholarly journals Characterization of the microbiome and bioluminescent symbionts across life stages of Ceratioid Anglerfishes of the Gulf of Mexico

2019 ◽  
Vol 95 (10) ◽  
Author(s):  
Lindsay L Freed ◽  
Cole Easson ◽  
Lydia J Baker ◽  
Danté Fenolio ◽  
Tracey T Sutton ◽  
...  
Keyword(s):  
Gulf Of Mexico ◽  
16S Rdna ◽  
Bacterial Communities ◽  
Host Family ◽  
Transmission Electron ◽  
Rdna Sequencing ◽  
Light Organs ◽  
Adult Skin ◽  
Host Development

ABSTRACT The interdependence of diverse organisms through symbiosis reaches even the deepest parts of the oceans. As part of the DEEPEND project (deependconsortium.org) research on deep Gulf of Mexico biodiversity, we profiled the bacterial communities (‘microbiomes’) and luminous symbionts of 36 specimens of adult and larval deep-sea anglerfishes of the suborder Ceratioidei using 16S rDNA. Transmission electron microscopy was used to characterize the location of symbionts in adult light organs (esca). Whole larval microbiomes, and adult skin and gut microbiomes, were dominated by bacteria in the genera Moritella and Pseudoalteromonas. 16S rDNA sequencing results from adult fishes corroborate the previously published identity of ceratioid bioluminescent symbionts and support the findings that these symbionts do not consistently exhibit host specificity at the host family level. Bioluminescent symbiont amplicon sequence variants were absent from larval ceratioid samples, but were found at all depths in the seawater, with a highest abundance found at mesopelagic depths. As adults spend the majority of their lives in the meso- and bathypelagic zones, the trend in symbiont abundance is consistent with their life history. These findings support the hypothesis that bioluminescent symbionts are not present throughout host development, and that ceratioids acquire their bioluminescent symbionts from the environment.

scholarly journals ISOLATION, PURIFICATION, AND CHARACTERIZATION OF LIPASE FROM BACILLUS SP. FROM KITCHEN GREASE

2018 ◽  
Vol 11 (6) ◽  
pp. 224
Author(s):  
Jaiganesh R ◽  
Jaganathan Mk
Keyword(s):  
16S Rdna ◽  
Solvent Tolerance ◽  
16S Rdna Sequencing ◽  
Bacillus Sp ◽  
Purification And Characterization ◽  
Lipase Enzyme ◽  
Sequencing Method ◽  
Rdna Sequencing ◽  
Solvent Tolerant

Objective: The objective of this work was to isolation, purification and characterization of solvent tolerant lipase from Bacillus sp. The objective of this work was to isolation, purification and characterization of solvent tolerant lipase from Bacillus sp. from kitchen grease for a variety of applications including organic synthetic reactions and preparation of enantiomerically pure pharmaceuticals.Methods: Lipase producing isolates were screened from kitchen grease on a selective medium rhodamine B olive oil agar, and tributyrin agar was used to screen the lipase and esterase producing an organism, respectively. The isolate identified using 16S rDNA sequencing method and enzyme activity was quantitatively assayed. Lipase production was characterized in different conditions.Results: The isolate showed highest lipase activity was which later was identified as Bacillus sp. using 16S rDNA sequencing method. The lipase was purified using ammonium sulfate precipitation. The isolate showed excellent tolerance to methanol, ethanol, acetonitrile, and moderate tolerance to butanol. The increased biomass concentration, maximum production, and activity were achieved at 37°C in 24 h incubation, then gradual reduction in production was observed. The maximum activity of lipase enzyme was obtained at pH between 6 and 9.Conclusion: The isolate produce solvent tolerance lipase enzyme and it can be a promising candidate of solvent tolerance lipase enzyme for variety of industrial applications.

scholarly journals Characterization of Bacterial Communities in Selected Smokeless Tobacco Products Using 16S rDNA Analysis

PLoS ONE ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. e0146939 ◽  
Author(s):  
Robert E. Tyx ◽  
Stephen B. Stanfill ◽  
Lisa M. Keong ◽  
Angel J. Rivera ◽  
Glen A. Satten ◽  
...  
Keyword(s):  
16S Rdna ◽  
Smokeless Tobacco ◽  
Bacterial Communities ◽  
Tobacco Products ◽  
16S Rdna Analysis ◽  
Smokeless Tobacco Products ◽  
Rdna Analysis

Study of bacterial communities in Antarctic coastal waters by a combination of 16S rRNA and 16S rDNA sequencing

2006 ◽  
Vol 8 (12) ◽  
pp. 2150-2161 ◽  
Author(s):  
Gabriella Gentile ◽  
Laura Giuliano ◽  
Giuseppe D'Auria ◽  
Francesco Smedile ◽  
Maurizio Azzaro ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rdna ◽  
Coastal Waters ◽  
Bacterial Communities ◽  
16S Rdna Sequencing ◽  
Rdna Sequencing

Physiological and genetic characterization of fluorescentPseudomonasassociated withCantharellus cibarius

2002 ◽  
Vol 48 (8) ◽  
pp. 739-748 ◽  
Author(s):  
J Ignacio Rangel-Castro ◽  
Jolanta J Levenfors ◽  
Eric Danell
Keyword(s):  
16S Rdna ◽  
Fungal Mycelium ◽  
Sequencing Analysis ◽  
Carbon Utilization ◽  
Pseudomonas Spp ◽  
Fluorescent Pseudomonas ◽  
Rdna Sequencing ◽  
Pcr Rflp ◽  
Polymerase Chain

Fluorescent Pseudomonas spp. isolated from fruiting bodies (FB) of Cantharellus cibarius were characterized physiologically and genetically and were compared with fluorescent Pseudomonas from forest soil and with sequences from the GenBank database. Pseudomonas spp. from FB differed physiologically from isolates from soil lacking FB and had some similarities with the strains obtained from soil underneath the FB. Analyses of the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) patterns and partial sequencing analysis of the 16S-rDNA region indicated that the bacteria isolated from these environments were different. However, there was no specific Pseudomonas genotype restricted to the FB environment. Utilization of the reported fungal exudates trehalose and mannitol may explain how millions of bacteria survive in the C. cibarius FB without deteriorating the fungal mycelium. The importance of the metabolic characterization of bacteria and the possible mechanisms involved in the association with C. cibarius are discussed. Our study showed that standard processes for bacterial identification, e.g., Biolog®and 16S-rDNA are insufficient until databases for different ecosystems are created.Key words: Cantharellus cibarius, fluorescent Pseudomonas, carbon utilization, PCR–RFLP, 16S-rDNA sequencing.

Characterization of Viable Bacteria from Siberian Permafrost by 16S rDNA Sequencing

1997 ◽  
Vol 33 (3) ◽  
pp. 169-179 ◽  
Author(s):  
T. Shi ◽  
R. H. Reeves ◽  
D. A. Gilichinsky ◽  
E. I. Friedmann
Keyword(s):  
16S Rdna ◽  
16S Rdna Sequencing ◽  
Rdna Sequencing ◽  
Viable Bacteria ◽  
Siberian Permafrost

scholarly journals Characterization of Microbial Communities in a Dairy Farm Matrix in Ningxia, China, by 16S rDNA Analysis

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Wen Zhang ◽  
Wu Li ◽  
Chenjie Ma ◽  
Xiaoling Wu ◽  
Xunde Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
16S Rdna ◽  
Bacterial Communities ◽  
Pathogenic Bacteria ◽  
Bacterial Community Composition ◽  
Dairy Farm ◽  
Dairy Manure ◽  
Zoonotic Pathogens ◽  
Rdna Sequencing ◽  
The Impact

A large amount of dairy manure is produced annually in the Ningxia Hui Autonomous Region of China due to the increase in food-producing animal agriculture in this region. The presence of bovine-originated zoonotic, especially human, pathogenic bacteria in untreated manure poses a significant threat to the environment and to public health. However, little is known about the composition, diversity, and abundance of bacterial communities in untreated dairy manure in the Ningxia region. In this study, the microbial community structure of the dairy farm matrix was characterized through 16S rDNA sequencing. The impact of manure treatment methods on bacterial communities was also analyzed. The results showed that the microbial community in dairy manure contained both beneficial bacteria and pathogens, with Firmicutes, Bacteroidetes, Proteobacteria, Spirochaetes, and Actinobacteria as dominant phyla. The results also showed the diversity and variety of abundance of zoonotic pathogens among different matrices. The number of pathogens was found to increase significantly in the accumulated but untreated manure, which appeared to be the main matrix of dairy farms that accumulated pathogens including zoonotic pathogens. Findings from this study suggested that farm management, particularly proper treatment of manure, is essential to achieve a shift in the bacterial community composition and a reduction in the environmental load of pathogens including zoonotic pathogens.

scholarly journals Characterization of the Plant-Associated Bacterial Microbiota of the Mexican Medicinal Species Bouvardia ternifolia

Proceedings ◽  
2021 ◽  
Vol 66 (1) ◽  
pp. 34
Author(s):  
Loan Edel Villalobos-Flores ◽  
Samuel David Espinosa-Torres ◽  
Fernando Hernández-Quiroz ◽  
Alberto Piña-Escobedo ◽  
Yair Cruz-Narváez ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Bacterial Communities ◽  
Plant Tissues ◽  
Medicinal Species ◽  
Potential Source ◽  
Bacterial Microbiota ◽  
Rdna Sequencing ◽  
Medicinal Properties ◽  
Therapeutic Compounds

Though therapeutic compounds can be extracted directly from medicinal plant tissues; it is now known that many phytotherapeutic compounds are actually produced by associated microorganisms or due to their interaction with the plant. Bouvardia ternifolia is a medicinal plant that can be considered a potential source of therapeutic compounds, such as the antitumoral molecule bouvardin. We characterized the endophytic, endophytic+epiphyte and soil bacterial microbiota in the flower, leaves, stems and roots in specimens of this plant through 16S rDNA sequencing. We found that Proteobacteria followed by Actinobacteria were the most abundant bacteria phyla in this plant. On the other hand, the most representative genera living endophytically were Propionibacterium, Paraccocus, Lactobacillus, Kaistobacter, Methylobacterium and Erwinia. This study provides evidence of the composition and diversity of the bacterial communities present in soil and tissues of B. ternifolia which can be considered for subsequent analysis and understanding of the therapeutic compounds that give this species its medicinal properties.

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