scholarly journals Production of long-chain hydroxy fatty acids by Starmerella bombicola

2019 ◽  
Vol 19 (7) ◽  
Author(s):  
Marilyn De Graeve ◽  
Isabelle Van de Velde ◽  
Lien Saey ◽  
Maarten Chys ◽  
Hanne Oorts ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dicarboxylic Acids ◽  
Strain Engineering ◽  
Microbial Production ◽  
Hydroxy Fatty Acids ◽  
Flash Chromatography ◽  
Starmerella Bombicola ◽  
Production Platform ◽  
The One ◽  
Fatty Aldehydes

ABSTRACT To decrease our dependency for the diminishing source of fossils resources, bio-based alternatives are being explored for the synthesis of commodity and high-value molecules. One example in this ecological initiative is the microbial production of the biosurfactant sophorolipids by the yeast Starmerella bombicola. Sophorolipids are surface-active molecules mainly used as household and laundry detergents. Because S. bombicola is able to produce high titers of sophorolipids, the yeast is also used to increase the portfolio of lipophilic compounds through strain engineering. Here, the one-step microbial production of hydroxy fatty acids by S. bombicola was accomplished by the selective blockage of three catabolic pathways through metabolic engineering. Successful production of 17.39 g/l (ω-1) linked hydroxy fatty acids was obtained by the successive blockage of the sophorolipid biosynthesis, the β-oxidation and the ω-oxidation pathways. Minor contamination of dicarboxylic acids and fatty aldehydes were successfully removed using flash chromatography. This way, S. bombicola was further expanded into a flexible production platform of economical relevant compounds in the chemical, food and cosmetic industries.

Microbial production of hydroxy fatty acids utilizing crude glycerol

2022 ◽  
pp. 102286
Author(s):  
Naomi Murakawa ◽  
Takaiku Sakamoto ◽  
Mizuho Kanoh ◽  
Si-Bum Park ◽  
Shigenobu Kishino ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Crude Glycerol ◽  
Microbial Production ◽  
Hydroxy Fatty Acids

scholarly journals Engineering precursor pools for increasing production of odd-chain fatty acids in Yarrowia lipolytica

2020 ◽  
Author(s):  
Young-Kyoung Park ◽  
Florence Bordes ◽  
Fabien Letisse ◽  
Jean-Marc Nicaud
Keyword(s):  
Fatty Acids ◽  
Yarrowia Lipolytica ◽  
Carbon Chain ◽  
Strain Engineering ◽  
Microbial Production ◽  
Total Lipids ◽  
Coa Transferase ◽  
Wide Range ◽  
Odd Chain Fatty Acids ◽  
Chain Fatty Acids

AbstractMicrobial production of lipids is one of the promising alternatives to fossil fuels with increasing environmental and energy concern. Odd-chain fatty acids (OCFA), a type of unusual lipids, are recently gaining a lot of interest as target compounds in microbial production due to their diverse applications in the medical, pharmaceutical, and chemical industries. In this study, we aimed to enhance the pool of precursors with three-carbon chain (propionyl-CoA) and five-carbon chain (β-ketovaleryl-CoA) for the production of OCFAs in Yarrowia lipolytica. We evaluated different propionate-activating enzymes and the overexpression of propionyl-CoA transferase gene from Ralstonia eutropha increased the accumulation of OCFAs by 3.8 times over control strain, indicating propionate activation is the limiting step of OCFAs synthesis. It was shown that acetate supplement was necessary to restore growth and to produce a higher OCFA contents in total lipids, suggesting the balance of the precursors between acetyl-CoA and propionyl-CoA is crucial for OCFA accumulation. To improve β-ketovaleryl-CoA pools for further increase of OCFA production, we co-expressed the bktB encoding β-ketothiolase in the producing strain, and the OCFA production was increased by 33 % compared to control. Combining strain engineering and the optimization of the C/N ratio promoted the OCFA production up to 1.87 g/L representing 62% of total lipids, the highest recombinant OCFAs titer reported in yeast, up to date. This study provides a strong basis for the microbial production of OCFAs and its derivatives having high potentials in a wide range of applications.

Dicarboxylic acids and hydroxy fatty acids in different species of fungi

2016 ◽  
Vol 71 (5) ◽  
pp. 999-1005
Author(s):  
Aleksandra Ostachowska ◽  
Piotr Stepnowski ◽  
Marek Gołębiowski
Keyword(s):  
Fatty Acids ◽  
Dicarboxylic Acids ◽  
Hydroxy Fatty Acids

EngineeringEscherichia colifor Conversion of Glucose to Medium-Chain ω-Hydroxy Fatty Acids and α,ω-Dicarboxylic Acids

2015 ◽  
Vol 5 (3) ◽  
pp. 200-206 ◽  
Author(s):  
Christopher H. Bowen ◽  
Jeff Bonin ◽  
Anna Kogler ◽  
Carlos Barba-Ostria ◽  
Fuzhong Zhang
Keyword(s):  
Fatty Acids ◽  
Dicarboxylic Acids ◽  
Hydroxy Fatty Acids ◽  
Medium Chain

scholarly journals Cloning and Characterization of Three Fatty Alcohol Oxidase Genes from Candida tropicalis Strain ATCC 20336

2004 ◽  
Vol 70 (8) ◽  
pp. 4872-4879 ◽  
Author(s):  
L. Dudley Eirich ◽  
David L. Craft ◽  
Lisa Steinberg ◽  
Afreen Asif ◽  
William H. Eschenfeldt ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Amino Acid ◽  
Substrate Specificity ◽  
Fatty Alcohol ◽  
Candida Tropicalis ◽  
Sequence Homology ◽  
Alcohol Oxidase ◽  
Dicarboxylic Acids ◽  
Hydroxy Fatty Acids ◽  
E Coli

ABSTRACT Candida tropicalis (ATCC 20336) converts fatty acids to long-chain dicarboxylic acids via a pathway that includes among other reactions the oxidation of ω-hydroxy fatty acids to ω-aldehydes by a fatty alcohol oxidase (FAO). Three FAO genes (one gene designated FAO1 and two putative allelic genes designated FAO2a and FAO2b), have been cloned and sequenced from this strain. A comparison of the DNA sequence homology and derived amino acid sequence homology between these three genes and previously published Candida FAO genes indicates that FAO1 and FAO2 are distinct genes. Both genes were individually cloned and expressed in Escherichia coli. The substrate specificity and K m values for the recombinant FAO1 and FAO2 were significantly different. Particularly striking is the fact that FAO1 oxidizes ω-hydroxy fatty acids but not 2-alkanols, whereas FAO2 oxidizes 2-alkanols but not ω-hydroxy fatty acids. Analysis of extracts of strain H5343 during growth on fatty acids indicated that only FAO1 was highly induced under these conditions. FAO2 contains one CTG codon, which codes for serine (amino acid 177) in C. tropicalis but codes for leucine in E. coli. An FAO2a construct, with a TCG codon (codes for serine in E. coli) substituted for the CTG codon, was prepared and expressed in E. coli. Neither the substrate specificity nor the K m values for the FAO2a variant with a serine at position 177 were radically different from those of the variant with a leucine at that position.

scholarly journals A non-canonical Δ9-desaturase synthesizing palmitoleic acid identified in the thraustochytrid Aurantiochytrium sp. T66

2021 ◽  
Author(s):  
E-Ming Rau ◽  
Inga Marie Aasen ◽  
Helga Ertesvåg
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Palmitoleic Acid ◽  
Unsaturated Fatty Acids ◽  
Gene Annotation ◽  
Vaccenic Acid ◽  
Strain Engineering ◽  
Δ9 Desaturase ◽  
Δ12 Desaturase

Abstract Thraustochytrids are oleaginous marine eukaryotic microbes currently used to produce the essential omega-3 fatty acid docosahexaenoic acid (DHA, C22:6 n-3). To improve the production of this essential fatty acid by strain engineering, it is important to deeply understand how thraustochytrids synthesize fatty acids. While DHA is synthesized by a dedicated enzyme complex, other fatty acids are probably synthesized by the fatty acid synthase, followed by desaturases and elongases. Which unsaturated fatty acids are produced differs between different thraustochytrid genera and species; for example, Aurantiochytrium sp. T66, but not Aurantiochytrium limacinum SR21, synthesizes palmitoleic acid (C16:1 n-7) and vaccenic acid (C18:1 n-7). How strain T66 can produce these fatty acids has not been known, because BLAST analyses suggest that strain T66 does not encode any Δ9-desaturase-like enzyme. However, it does encode one Δ12-desaturase-like enzyme. In this study, the latter enzyme was expressed in A. limacinum SR21, and both C16:1 n-7 and C18:1 n-7 could be detected in the transgenic cells. Our results show that this desaturase, annotated T66Des9, is a Δ9-desaturase accepting C16:0 as a substrate. Phylogenetic studies indicate that the corresponding gene probably has evolved from a Δ12-desaturase-encoding gene. This possibility has not been reported earlier and is important to consider when one tries to deduce the potential a given organism has for producing unsaturated fatty acids based on its genome sequence alone. Key points • In thraustochytrids, automatic gene annotation does not always explain the fatty acids produced. • T66Des9 is shown to synthesize palmitoleic acid (C16:1 n-7). • T66des9 has probably evolved from Δ12-desaturase-encoding genes.

scholarly journals Microbial production of polyunsaturated fatty acids — high-value ingredients for aquafeed, superfoods, and pharmaceuticals

2021 ◽  
Vol 69 ◽  
pp. 199-211
Author(s):  
Sofija Jovanovic ◽  
Demian Dietrich ◽  
Judith Becker ◽  
Michael Kohlstedt ◽  
Christoph Wittmann
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Microbial Production
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