Microbial production of hydroxy fatty acids utilizing crude glycerol

2022 ◽  
pp. 102286
Author(s):  
Naomi Murakawa ◽  
Takaiku Sakamoto ◽  
Mizuho Kanoh ◽  
Si-Bum Park ◽  
Shigenobu Kishino ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Crude Glycerol ◽  
Microbial Production ◽  
Hydroxy Fatty Acids

scholarly journals Production of long-chain hydroxy fatty acids by Starmerella bombicola

2019 ◽  
Vol 19 (7) ◽  
Author(s):  
Marilyn De Graeve ◽  
Isabelle Van de Velde ◽  
Lien Saey ◽  
Maarten Chys ◽  
Hanne Oorts ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dicarboxylic Acids ◽  
Strain Engineering ◽  
Microbial Production ◽  
Hydroxy Fatty Acids ◽  
Flash Chromatography ◽  
Starmerella Bombicola ◽  
Production Platform ◽  
The One ◽  
Fatty Aldehydes

ABSTRACT To decrease our dependency for the diminishing source of fossils resources, bio-based alternatives are being explored for the synthesis of commodity and high-value molecules. One example in this ecological initiative is the microbial production of the biosurfactant sophorolipids by the yeast Starmerella bombicola. Sophorolipids are surface-active molecules mainly used as household and laundry detergents. Because S. bombicola is able to produce high titers of sophorolipids, the yeast is also used to increase the portfolio of lipophilic compounds through strain engineering. Here, the one-step microbial production of hydroxy fatty acids by S. bombicola was accomplished by the selective blockage of three catabolic pathways through metabolic engineering. Successful production of 17.39 g/l (ω-1) linked hydroxy fatty acids was obtained by the successive blockage of the sophorolipid biosynthesis, the β-oxidation and the ω-oxidation pathways. Minor contamination of dicarboxylic acids and fatty aldehydes were successfully removed using flash chromatography. This way, S. bombicola was further expanded into a flexible production platform of economical relevant compounds in the chemical, food and cosmetic industries.

scholarly journals Microbial production of polyunsaturated fatty acids — high-value ingredients for aquafeed, superfoods, and pharmaceuticals

2021 ◽  
Vol 69 ◽  
pp. 199-211
Author(s):  
Sofija Jovanovic ◽  
Demian Dietrich ◽  
Judith Becker ◽  
Michael Kohlstedt ◽  
Christoph Wittmann
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Microbial Production

scholarly journals Occurrence of 2-hydroxy fatty acids in animal tissues

1963 ◽  
Vol 4 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Yasuo Kishimoto ◽  
Norman S. Radin
Keyword(s):  
Fatty Acids ◽  
Hydroxy Fatty Acids ◽  
Animal Tissues

scholarly journals Optimization and Engineering of a Self-Sufficient CYP102 Enzyme from Bacillus amyloliquefaciens towards Synthesis of In-Chain Hydroxy Fatty Acids

Catalysts ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 665
Author(s):  
Li Zong ◽  
Yan Zhang ◽  
Zhengkang Shao ◽  
Yingwu Wang ◽  
Zheng Guo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Palmitic Acid ◽  
Bacillus Amyloliquefaciens ◽  
Catalytic Domain ◽  
Hydroxy Fatty Acids ◽  
Saturation Mutagenesis ◽  
Long Chain Fatty Acids ◽  
Medical Field ◽  
Biotransformation Process ◽  
Total Product

Cytochrome P450 (CYP) mediated enzymatic hydroxylation of fatty acids present a green alternative to chemical synthesis of hydroxy fatty acids (HFAs), which are high-value oleochemicals with various uses in materials industry and medical field. Although many CYPs require the presence of additional reductase proteins for catalytic activity, self-sufficient CYPs have their reductase partner naturally fused into their catalytic domain, leading to a greatly simplified biotransformation process. A recently discovered self-sufficient CYP, BAMF2522 from Bacillus amyloliquefaciens DSM 7, exhibits novel regioselectivity by hydroxylating in-chain positions of palmitic acid generating ω-1 to ω-7 HFAs, a rare regiodiversity profile among CYPs. Besides, F89I mutant of BAMF2522 expanded hydroxylation up to ω-9 position of palmitic acid. Here, we further characterize this enzyme by determining optimum temperature and pH as well as thermal stability. Moreover, using extensive site-directed and site-saturation mutagenesis, we obtained BAMF2522 variants that demonstrate greatly increased regioselectivity for in-chain positions (ω-4 to ω-9) of various medium to long chain fatty acids. Remarkably, when a six-residue mutant was reacted with palmitic acid, 84% of total product content was the sum of ω-7, ω-8 and ω-9 HFA products, the highest in-chain selectivity observed to date with a self-sufficient CYP. In short, our study demonstrates the potential of a recently identified CYP and its mutants for green and sustainable production of a variety of in-chain hydroxy enriched HFAs.

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