Assessing the supercooling of fresh-cut onions at −5°C using electrical impedance analysis

Abstract We supercooled fresh-cut onion at −5°C for 12 h. After supercooling, the electric impedance properties of the samples were evaluated by electrical impedance spectroscopy over the frequency range of 42 Hz − 5 MHz. The time-temperature profiles of samples indicated that the freezing point and supercooling point were −2.3°C ± 0.7°C and −6.9°C ± 1.0°C, respectively. The results indicated that 34 of the 36 supercooled samples exhibited a definite circular arc in the Cole-Cole plot, which suggested that the cell membrane remained intact during supercooling. In the other two samples which did not exhibit a definite circular arc, the cell membrane had sustained serious damage during supercooling. Furthermore, there was large difference in drip loss percentage between supercooled samples exhibited a definite circular arc in the Cole-Cole plot and samples not exhibiting a definite circular arc. Our results suggest that fresh-cut onions can be supercooled at −5°C.

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Electrical Impedance Analysis of Phospholipid Bilayer Membranes for Enabling Engineering Design of Bio-based Devices

MRS Proceedings ◽

10.1557/proc-1061-mm03-18 ◽

2007 ◽

Vol 1061 ◽

Author(s):

Stephen A. Sarles ◽

Vishnu B. Sundaresan ◽

Donald J. Leo

Keyword(s):

Lipid Membranes ◽

Electrical Impedance ◽

Lipid Membrane ◽

Electrical Power ◽

Impedance Analysis ◽

Electrical Impedance Spectroscopy ◽

Phospholipid Bilayer ◽

Silicon Substrates ◽

Bilayer Membranes ◽

Single Aperture

ABSTRACTRecent research at Virginia Tech have shown that active transporter proteins reconstituted into suspended bilayer lipid membranes (BLMs) formed across an array of pores in synthetic substrates can convert chemical energy available in adenosine triphosphate (ATP) into electricity. Experimental results from this work show that this system—called BioCell—is capable of 1.7μW of electrical power per square centimeter of BLM area and per 15μL of ATPase enzyme. In support of such a system, the lipid membrane, as host to active biological proteins and channels, must be formed evenly across a porous substrate, remain stable and yet fluid-like for protein folding and activation, and provide sufficient electrical insulation. We report on the formation and characterization using electrical impedance spectroscopy (EIS) of BLMs formed across two types of porous substrates: polycarbonate filters and single-aperture silicon substrates. Equivalent electrical circuits describing the lipid membranes and their supporting substrates are approximated to fit the measured responses. The results show that BLMs formed in some but not all of the 400nm pores of the filters, while the formation of BLMs on the single-aperture silicon substrates was much more consistent.

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Evaluation of Freshness of Fresh-cut Lettuce based on Electrical Impedance Analysis

IEEJ Transactions on Fundamentals and Materials ◽

10.1541/ieejfms.136.613 ◽

2016 ◽

Vol 136 (10) ◽

pp. 613-620

Author(s):

Yasumasa Ando ◽

Yuka Maeda ◽

Koichi Mizutani ◽

Naoto Wakatsuki

Keyword(s):

Electrical Impedance ◽

Impedance Analysis ◽

Fresh Cut

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Bioelectrical Impedance Methods for Noninvasive Health Monitoring: A Review

Journal of Medical Engineering ◽

10.1155/2014/381251 ◽

2014 ◽

Vol 2014 ◽

pp. 1-28 ◽

Cited By ~ 99

Author(s):

Tushar Kanti Bera

Keyword(s):

Electrical Impedance ◽

Tissue Characterization ◽

Bioelectrical Impedance ◽

Impedance Analysis ◽

Biological Tissues ◽

Disease Diagnosis ◽

Impedance Plethysmography ◽

Electrical Impedance Spectroscopy ◽

Signal Frequency ◽

Impedance Tomography

Under the alternating electrical excitation, biological tissues produce a complex electrical impedance which depends on tissue composition, structures, health status, and applied signal frequency, and hence the bioelectrical impedance methods can be utilized for noninvasive tissue characterization. As the impedance responses of these tissue parameters vary with frequencies of the applied signal, the impedance analysis conducted over a wide frequency band provides more information about the tissue interiors which help us to better understand the biological tissues anatomy, physiology, and pathology. Over past few decades, a number of impedance based noninvasive tissue characterization techniques such as bioelectrical impedance analysis (BIA), electrical impedance spectroscopy (EIS), electrical impedance plethysmography (IPG), impedance cardiography (ICG), and electrical impedance tomography (EIT) have been proposed and a lot of research works have been conducted on these methods for noninvasive tissue characterization and disease diagnosis. In this paper BIA, EIS, IPG, ICG, and EIT techniques and their applications in different fields have been reviewed and technical perspective of these impedance methods has been presented. The working principles, applications, merits, and demerits of these methods has been discussed in detail along with their other technical issues followed by present status and future trends.

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Electrical Impedance Spectroscopy Estimation of Water Content in Leaves and Stems of White Birch (Betula platyphylla) Under Flooding Stress

CHINESE BULLETIN OF BOTANY ◽

10.3724/sp.j.1259.2014.00105 ◽

2014 ◽

Vol 49 (1) ◽

pp. 105 ◽

Cited By ~ 1

Author(s):

Meng Yu ◽

Gong Ruijuan ◽

Di Bao ◽

Xiang Diying ◽

Zhang Gang ◽

...

Keyword(s):

Impedance Spectroscopy ◽

Water Content ◽

Electrical Impedance ◽

Electrical Impedance Spectroscopy ◽

Betula Platyphylla ◽

White Birch ◽

Flooding Stress

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Monitoring Contractile Cardiomyocytes via Impedance Using Multipurpose Thin Film Ruthenium Oxide Electrodes

Sensors ◽

10.3390/s21041433 ◽

2021 ◽

Vol 21 (4) ◽

pp. 1433

Author(s):

Esther Tanumihardja ◽

Douwe S. de Bruijn ◽

Rolf H. Slaats ◽

Wouter Olthuis ◽

Albert van den Berg

Keyword(s):

Thin Film ◽

Electrical Impedance ◽

Ruthenium Oxide ◽

Electrical Impedance Spectroscopy ◽

Visual Data ◽

Oxide Electrodes ◽

Pt Electrodes ◽

Mode Of Operation ◽

Frequency Components

A ruthenium oxide (RuOx) electrode was used to monitor contractile events of human pluripotent stem cells-derived cardiomyocytes (hPSC-CMs) through electrical impedance spectroscopy (EIS). Using RuOx electrodes presents an advantage over standard thin film Pt electrodes because the RuOx electrodes can also be used as electrochemical sensor for pH, O2, and nitric oxide, providing multisensory functionality with the same electrode. First, the EIS signal was validated in an optically transparent well-plate setup using Pt wire electrodes. This way, visual data could be recorded simultaneously. Frequency analyses of both EIS and the visual data revealed almost identical frequency components. This suggests both the EIS and visual data captured the similar events of the beating of (an area of) hPSC-CMs. Similar EIS measurement was then performed using the RuOx electrode, which yielded comparable signal and periodicity. This mode of operation adds to the versatility of the RuOx electrode’s use in in vitro studies.

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A Rapid Approach to Measure Extracted Chlorophyll-a from Lettuce Leaves using Electrical Impedance Spectroscopy

Water Air & Soil Pollution ◽

10.1007/s11270-021-05036-z ◽

2021 ◽

Vol 232 (2) ◽

Author(s):

Rakibul Islam Chowdhury ◽

Rinku Basak ◽

Khan Arif Wahid ◽

Katy Nugent ◽

Helen Baulch

Keyword(s):

Impedance Spectroscopy ◽

Chlorophyll A ◽

Electrical Impedance ◽

Electrical Impedance Spectroscopy

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Study of Reduced Graphene Oxide Dispersions via Electrical Impedance Spectroscopy

Procedia Structural Integrity ◽

10.1016/j.prostr.2020.10.142 ◽

2020 ◽

Vol 28 ◽

pp. 1679-1685

Author(s):

Angeliki-Eirini Dimou ◽

Ioanna Sakellariou ◽

George M. Maistros ◽

Nikolaos D. Alexopoulos

Keyword(s):

Graphene Oxide ◽

Impedance Spectroscopy ◽

Reduced Graphene Oxide ◽

Electrical Impedance ◽

Electrical Impedance Spectroscopy ◽

Reduced Graphene

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Utilization of Electrical Impedance Spectroscopy and Image Classification for Non-Invasive Early Assessment of Meat Freshness

Sensors ◽

10.3390/s21031001 ◽

2021 ◽

Vol 21 (3) ◽

pp. 1001

Author(s):

Sooin Huh ◽

Hye-Jin Kim ◽

Seungah Lee ◽

Jinwoo Cho ◽

Aera Jang ◽

...

Keyword(s):

Impedance Spectroscopy ◽

Prediction Accuracy ◽

Electrical Impedance ◽

Thiobarbituric Acid ◽

Electrical Impedance Spectroscopy ◽

Plate Count ◽

Gradient Boosting ◽

Composition Analysis ◽

Early Assessment ◽

Relative Standard

This study presents a system for assessing the freshness of meat with electrical impedance spectroscopy (EIS) in the frequency range of 125 Hz to 128 kHz combined with an image classifier for non-destructive and low-cost applications. The freshness standard is established by measuring the aerobic plate count (APC), 2-thiobarbituric acid reactive substances (TBARS), and composition analysis (crude fat, crude protein, and moisture) values of the microbiological detection to represent the correlation between EIS and meat freshness. The EIS and images of meat are combined to predict the freshness with the Adaboost classification and gradient boosting regression algorithms. As a result, when the elapsed time of beef storage for 48 h is classified into three classes, the time prediction accuracy is up to 85% compared to prediction accuracy of 56.7% when only images are used without EIS information. Significantly, the relative standard deviation (RSD) of APC and TBARS value predictions with EIS and images datum achieves 0.890 and 0.678, respectively.

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