scholarly journals REGULATION OF MOUSE MAJOR URINARY PROTEIN PRODUCTION BY THE MUP-A GENE

Genetics ◽  
1978 ◽  
Vol 90 (3) ◽  
pp. 597-612
Author(s):  
P R Szoka ◽  
K Paigen
Keyword(s):  
Protein Production ◽  
Inbred Strains ◽  
Electrophoretic Separation ◽  
Urinary Proteins ◽  
Major Urinary Protein ◽  
Testosterone Treatment ◽  
Major Urinary Proteins ◽  
Before And After ◽  
Daily Excretion ◽  
Induced Proteins

ABSTRACT A method was developed to quantitate the daily excretion of the three major urinary proteins (mups) to test which parameters of the mup phenotype are controlled by the the Mup-a gene. Electrophoretic separation of the mup proteins, followed by staining and spectrophotometric scanning was used to characterize the phenotypes of various inbred strains. The mup phenotype of a strain proved to have two components: the absolute levels and the relative proportions of the mups present in the urine. Testosterone treatment alters both components of the mup phenotype, increasing mup excretion and altering their relative proportions. The induced proteins are the same as the basal proteins as judged by electrophoretic mobility, molecular weight, and reactivity with antibody. All strains excrete all three mups when induced. The Mup-a gene appears to be a single, codominantly expressed regulatory locus that controls the induced proportions of the three proteins. However, other genes in addition to Mup-a participate in controlling the basal mup proportions, as well as individual and total mup levels before and after testosterone treatment.

scholarly journals Linkage between sperm abnormality level and major urinary protein phenotype in mice

1991 ◽  
Vol 57 (2) ◽  
pp. 135-138 ◽  
Author(s):  
Jozefa Styrna
Keyword(s):  
Inbred Strains ◽  
Chromosome 4 ◽  
Urinary Proteins ◽  
Major Urinary Protein ◽  
Major Urinary Proteins ◽  
Abnormal Sperm ◽  
Inbred Strains Of Mice ◽  
Sperm Abnormality ◽  
High Level ◽  
Very High

SummarySegregation of sperm abnormality level and the pattern of major urinary proteins (MUPs) were investigated in F2 and B1 hybrid males obtained from crosses involving two contrasting inbred strains of mice: CBA/Kw (Mup-1a1a, 3·3% abnormal sperm) and C57BL/Kw (Mup-1b1b, 21·9% abnormal sperm). In the progeny of both crosses mean levels of abnormal spermatozoa were significantly higher for males typed as Mup-1b1b than for heterozygous Mup-1a1b males. Moreover, all F2 hybrid males showing very high percentages of abnormal sperm were Mup-1b1bhomozygotes. Similarly, among B1 males with a high level of deformed spermatozoa, a statistically significant majority were Mup-1b1b genotypes. Our results suggest that at least two genes which influence sperm abnormality level are segregating in these crosses. Both appear to be recessive for high sperm abnormality level, and one shows weak linkage to Mup-1 on chromosome 4.

scholarly journals GENETIC REGULATION OF MUP PRODUCTION IN RECOMBINANT INBRED MICE

Genetics ◽  
1979 ◽  
Vol 93 (1) ◽  
pp. 173-181
Author(s):  
P R Szoka ◽  
K Paigen
Keyword(s):  
Recombinant Inbred ◽  
Inbred Mice ◽  
Genetic Regulation ◽  
Inbred Strains ◽  
Urinary Proteins ◽  
Major Urinary Proteins ◽  
Recombinant Inbred Mice ◽  
Inbred Strains Of Mice ◽  
Before And After ◽  
Kinetics Of

ABSTRACT Inbred strains of mice excrete all three major urinary proteins (mups) when induced by testosterone, but differ as to the relative proportions and total levels of each mup present. We have now determined the urinary mup pheno-types before and after testosterone treatment of seven recombinant inbred strains derived from progenitor strains exhibiting different mup phenotypes. The results codirm previous observations indicating that total control of mup protein production is a multigenic process. One locus, Mup-a on chromosome 4, determines the relative mup protein proportions after induction by testoster- one. Mup-a, together with other genetic sites, determines the basal mup proportions. Genes other than Mup-a determine the kinetics of mup induction and total mup excretion.

scholarly journals Structural genes of the mouse major urinary protein are on chromosome 4.

1982 ◽  
Vol 94 (2) ◽  
pp. 414-417 ◽  
Author(s):  
K Krauter ◽  
L Leinwand ◽  
P D'Eustachio ◽  
F Ruddle ◽  
J E Darnell
Keyword(s):  
Protein Synthesis ◽  
Sequence Analysis ◽  
Chromosome 4 ◽  
Structural Genes ◽  
Urinary Proteins ◽  
Coding Region ◽  
Somatic Cell Genetics ◽  
Major Urinary Protein ◽  
Major Urinary Proteins ◽  
Trans Regulation

The major urinary proteins (MUPs) of mouse are a family of at least three major proteins which are synthesized in the liver of all strains of mice. The relative levels of synthesis of these proteins with respect to each other in the presence of testosterone is regulated by the Mup-a locus located on chromosome 4. In an effort to determine the mechanism of this regulation in molecular terms, a cDNA clone containing most of the coding region of a MUP protein has been isolated and identified by partial DNA sequence analysis. Using a combination of hybridization analysis and somatic cell genetics, the structural gene family has been unambiguously mapped to mouse chromosome 4. These data suggest that Mup-a regulation operates in a cis fashion and that models proposing trans regulation of MUP protein synthesis are unlikely.

scholarly journals Differential expression in male and female mouse liver of very similar mRNAs specified by two group 1 major urinary protein genes.

1989 ◽  
Vol 9 (5) ◽  
pp. 2202-2207 ◽  
Author(s):  
I McIntosh ◽  
J O Bishop
Keyword(s):  
Mouse Liver ◽  
Hormonal Status ◽  
Oligonucleotide Probes ◽  
Urinary Proteins ◽  
Pairwise Similarity ◽  
Major Urinary Protein ◽  
Male And Female ◽  
Major Urinary Proteins ◽  
Different Levels ◽  
Group 1

The major urinary proteins of the mouse are encoded by a large multigene family composed of several distinct groups of genes distinguished by differences in sequence and expression characteristics. The genes in the largest group (group 1) show greater than 99% pairwise similarity in their exons. By hybridization between RNA and a specifically designed oligonucleotide, we confirmed that genes of this group are expressed mainly in the liver. By using additional gene-specific oligonucleotide probes, we have been able to distinguish between the species of mRNA corresponding to two of these genes and to measure their abundance in male and female liver. Both mRNAs are present in male liver at high but different levels. Both are also present in female liver, one at a much lower level than in the male and the second at a very low level indeed. Both are present at male levels in the livers of females induced with testosterone. These results show unequivocally that the expression of different group 1 Mup genes is differentially influenced by the hormonal status of the mouse.

Differential expression in male and female mouse liver of very similar mRNAs specified by two group 1 major urinary protein genes

1989 ◽  
Vol 9 (5) ◽  
pp. 2202-2207
Author(s):  
I McIntosh ◽  
J O Bishop
Keyword(s):  
Mouse Liver ◽  
Hormonal Status ◽  
Oligonucleotide Probes ◽  
Urinary Proteins ◽  
Pairwise Similarity ◽  
Major Urinary Protein ◽  
Male And Female ◽  
Major Urinary Proteins ◽  
Different Levels ◽  
Group 1

The major urinary proteins of the mouse are encoded by a large multigene family composed of several distinct groups of genes distinguished by differences in sequence and expression characteristics. The genes in the largest group (group 1) show greater than 99% pairwise similarity in their exons. By hybridization between RNA and a specifically designed oligonucleotide, we confirmed that genes of this group are expressed mainly in the liver. By using additional gene-specific oligonucleotide probes, we have been able to distinguish between the species of mRNA corresponding to two of these genes and to measure their abundance in male and female liver. Both mRNAs are present in male liver at high but different levels. Both are also present in female liver, one at a much lower level than in the male and the second at a very low level indeed. Both are present at male levels in the livers of females induced with testosterone. These results show unequivocally that the expression of different group 1 Mup genes is differentially influenced by the hormonal status of the mouse.

scholarly journals Major urinary protein levels are associated with social status and context in mouse social hierarchies

2017 ◽  
Vol 284 (1863) ◽  
pp. 20171570 ◽  
Author(s):  
Won Lee ◽  
Amber Khan ◽  
James P. Curley
Keyword(s):  
Social Status ◽  
Social Rank ◽  
Male Mice ◽  
Urinary Proteins ◽  
Major Urinary Protein ◽  
Social Hierarchies ◽  
Protein Levels ◽  
Major Urinary Proteins ◽  
Stable Linear ◽  
Lower Urinary

We have previously shown that male mice living in groups of 12 males establish and maintain stable linear social hierarchies with each individual having a defined social rank. However, it is not clear which social cues mice use to signal and recognize their relative social status within their hierarchy. In this study, we investigate how individual social status both in pairs and in groups affects the levels of major urinary proteins (MUPs) and specifically MUP20 in urine. We housed groups of adult outbred CD1 male mice in a complex social environment for three weeks and collected urine samples from all individuals repeatedly. We found that dominant males produce more MUPs than subordinates when housed in pairs and that the production of MUPs and MUP20 is significantly higher in alpha males compared with all other individuals in a social hierarchy. Furthermore, we found that hepatic mRNA expression of Mup3 and Mup20 is significantly higher in alpha males than in subordinate males. We also show that alpha males have lower urinary creatinine levels consistent with these males urinating more than others living in hierarchies. These differences emerged within one week of animals being housed together in social hierarchies. This study demonstrates that as males transition to become alpha males, they undergo physiological changes that contribute to communication of their social status that may have implications for the energetic demands of maintaining dominance.

The major urinary protein system in the rat

2014 ◽  
Vol 42 (4) ◽  
pp. 886-892 ◽  
Author(s):  
Guadalupe Gómez-Baena ◽  
Stuart D. Armstrong ◽  
Marie M. Phelan ◽  
Jane L. Hurst ◽  
Robert J. Beynon
Keyword(s):  
Chemical Communication ◽  
Rattus Norvegicus ◽  
Current Knowledge ◽  
Urinary Protein ◽  
Present Review ◽  
Urinary Proteins ◽  
Major Urinary Protein ◽  
Major Urinary Proteins ◽  
Brown Rat ◽  
Rats And Mice

The genomes of rats and mice both contain a cluster of multiple genes that encode small (18–20 kDa) eight-stranded β-barrel lipocalins that are expressed in multiple secretory tissues, some of which enter urine via hepatic biosynthesis. These proteins have been given different names, but are mostly generically referred to as MUPs (major urinary proteins). The mouse MUP cluster is increasingly well understood, and, in particular, a number of roles for MUPs in chemical communication between conspecifics have been established. By contrast, the literature on the rat orthologues is much less well developed and is fragmented. In the present review, we summarize current knowledge on the MUPs from the Norway (or brown) rat, Rattus norvegicus.

scholarly journals VARIATION IN THE MAJOR URINARY PROTEIN MULTIGENE FAMILY IN WILD-DERIVED MICE

Genetics ◽  
1985 ◽  
Vol 109 (3) ◽  
pp. 549-568
Author(s):  
Bonnie M Sampsell ◽  
William A Held
Keyword(s):  
Differential Expression ◽  
Multigene Family ◽  
Genomic Dna ◽  
Genomic Organization ◽  
Urinary Proteins ◽  
Major Urinary Protein ◽  
Cis Acting ◽  
Major Urinary Proteins ◽  
Species Analysis ◽  
Male To Female

ABSTRACT The levels of expression and genomic organization of genes coding for the major urinary proteins (MUPs) were examined in several stocks of wild-derived mice. Levels of MUP mRNA in the liver varied considerably with M. musculusBrno and M. castaneus males having several-fold more MUP RNA than inbred C57BL/6 males, whereas M. hortulanus, M. caroli and M. cervicolor displayed levels much lower than C57BL/6. Analysis of RNA with MUP cDNAs specific to two different subfamilies of MUP genes revealed that M. caroli and M. cervicolor primarily expressed a MUP mRNA that was less abundant in C57BL/6, suggesting differential expression of subfamilies of genes within the MUP multigene complex. Although inbred males usually have five-fold more MUP mRNA than inbred females, male to female ratios for wild-derived stocks ranged from one to several hundred. Southern blots of genomic DNA hybridized to MUP subfamily probes revealed differences in restriction fragment sizes as well as possible variation in the number of MUP genes in some species. Analysis of urinary proteins from hybrids between C57BL/6 and M. spretus suggested that low MUP expression in M. spretus females was due to cis-acting genetic elements.

scholarly journals Molecular heterogeneity in the Major Urinary Proteins of the house mouse Mus musculus

1996 ◽  
Vol 316 (1) ◽  
pp. 265-272 ◽  
Author(s):  
Duncan H. L. ROBERTSON ◽  
Kathleen A. COX ◽  
Simon J. GASKELL ◽  
Richard P. EVERSHED ◽  
Robert J. BEYNON
Keyword(s):  
Mass Spectrometry ◽  
Ion Exchange ◽  
Inbred Strains ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Mass Spectrometry Analysis ◽  
Molecular Heterogeneity ◽  
Major Protein ◽  
Urinary Proteins ◽  
Major Urinary Proteins

Major Urinary Proteins (MUPs) from different inbred strains of mouse have been analysed by high-resolution ion-exchange chromatography and mass spectrometry. MUPs from six strains were resolved chromatographically into four major protein peaks which characterized two distinct phenotypes, typified by the profiles obtained from the Balb/c and C57BL/6 inbred strains. A combination of ion-exchange chromatography and electrospray ionization mass spectrometry analysis of the MUPs from each strain identified five proteins, only one of which was common to both strains. The charge and mass data, together with N-terminal sequence analyses, were correlated with the masses of the proteins inferred from published cDNA sequences. Several members of the family of MUP sequences differ in only four positions, and in some circumstances the substitutions elicit a minimal change in protein mass (Lys/Gln; Lys/Glu). Peptide mapping with endopeptidase Lys-C, followed by matrix-assisted laser desorption ionization–time-of-flight mass spectrometry permitted identification of new MUPs that were correlated with partial cDNA sequence data. In the two strains there are at least 13 different MUPs, either observed or predicted, indicating the heterogeneity of expression of this group of proteins.

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