Deferoxamine B but Not Deferoxamine G1 Inhibits Cytokine Production in Murine Bone Marrow Macrophages

1995 ◽  
Vol 172 (2) ◽  
pp. 490-496 ◽  
Author(s):  
I. B. Autenrieth ◽  
E. Bohn ◽  
J. H. Ewald ◽  
J. Heesemann
Keyword(s):  
Bone Marrow ◽  
Cytokine Production ◽  
Murine Bone Marrow

scholarly journals The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8294 ◽  
Author(s):  
Adila Aipire ◽  
Mahepali Mahabati ◽  
Shanshan Cai ◽  
Xianxian Wei ◽  
Pengfei Yuan ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mouse Model ◽  
Side Effect ◽  
Cytokine Production ◽  
Human Monocyte ◽  
Glycyrrhiza Uralensis ◽  
Naive Mouse ◽  
Immunostimulatory Activity ◽  
Murine Bone Marrow ◽  
Tnf Α

Background The enhancement of immunity is very important for immunocompromised patients such as cancer patients with radiotherapy or chemotherapy. Glycyrrhiza uralensis has been used as food and medicine for a long history. G. uralensis polysaccharides (GUPS) were prepared and its immunostimulatory effects were investigated. Methods Human monocyte-derived dendritic cells (DCs) and murine bone marrow-derived DCs were treated with different concentrations of GUPS. The DCs maturation and cytokine production were analyzed by flow cytometry and ELISA, respectively. Inhibitors and Western blot were used to study the mechanism of GUPS. The immunostimulatory effects of GUPS were further evaluated by naïve mouse model and immunosuppressive mouse model induced by cyclophosphamide. Results GUPS significantly promoted the maturation and cytokine secretion of human monocyte-derived DCs and murine bone marrow-derived DCs through TLR4 and down-stream p38, JNK and NF-κB signaling pathways. Interestingly, the migration of GUPS treated-DCs to lymph node was increased. In the mouse model, GUPS increased IL-12 production in sera but not for TNF-α. Moreover, GUPS ameliorated the side effect of cyclophosphamide and improved the immunity of immunosuppressive mice induced by cyclophosphamide. These results suggested that GUPS might be used for cancer therapy to ameliorate the side effect of chemotherapy and enhance the immunity.

scholarly journals Sphingosine-1-Phosphate Receptor 2 Controls Podosome Components Induced by RANKL Affecting Osteoclastogenesis and Bone Resorption

Cells ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 17 ◽  
Author(s):  
Li-Chien Hsu ◽  
Sakamuri V. Reddy ◽  
Özlem Yilmaz ◽  
Hong Yu
Keyword(s):  
Bone Marrow ◽  
Bone Resorption ◽  
Bone Loss ◽  
Cytokine Production ◽  
Bone Marrow Cells ◽  
Proinflammatory Cytokine Production ◽  
Murine Bone Marrow ◽  
Sphingosine 1 Phosphate ◽  
Production Cell ◽  
Cell Chemotaxis

Proinflammatory cytokine production, cell chemotaxis, and osteoclastogenesis can lead to inflammatory bone loss. Previously, we showed that sphingosine-1-phosphate receptor 2 (S1PR2), a G protein coupled receptor, regulates inflammatory cytokine production and osteoclastogenesis. However, the signaling pathways regulated by S1PR2 in modulating inflammatory bone loss have not been elucidated. Herein, we demonstrated that inhibition of S1PR2 by a specific S1PR2 antagonist (JTE013) suppressed phosphoinositide 3-kinase (PI3K), mitogen-activated protein kinases (MAPKs), and nuclear factor kappa-B (NF-κB) induced by an oral bacterial pathogen, Aggregatibacter actinomycetemcomitans, and inhibited the release of IL-1β, IL-6, TNF-α, and S1P in murine bone marrow cells. In addition, shRNA knockdown of S1PR2 or treatment by JTE013 suppressed cell chemotaxis induced by bacteria-stimulated cell culture media. Furthermore, JTE013 suppressed osteoclastogenesis and bone resorption induced by RANKL in murine bone marrow cultures. ShRNA knockdown of S1PR2 or inhibition of S1PR2 by JTE013 suppressed podosome components, including PI3K, Src, Pyk2, integrin β3, filamentous actin (F-actin), and paxillin levels induced by RANKL in murine bone marrow cells. We conclude that S1PR2 plays an essential role in modulating proinflammatory cytokine production, cell chemotaxis, osteoclastogenesis, and bone resorption. Inhibition of S1PR2 signaling could be a novel therapeutic strategy for bone loss associated with skeletal diseases.

Author response for "CXCR4, but not CXCR3, drives CD8 T-cell entry into and migration through the murine bone marrow"

2018 ◽  
Author(s):  
Marieke Goedhart ◽  
Stephanie Gessel ◽  
Robbert van der Voort ◽  
Edith Slot ◽  
Beth Lucas ◽  
...  
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Cd8 T Cell ◽  
Cell Entry ◽  
Author Response ◽  
Murine Bone Marrow ◽  
And Migration

scholarly journals Phagocytosis and activation of bone marrow‐derived macrophages by Plasmodium falciparum gametocytes

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Yolanda Corbett ◽  
Silvia Parapini ◽  
Federica Perego ◽  
Valeria Messina ◽  
Serena Delbue ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Bone Marrow ◽  
Plasmodium Falciparum ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Murine Bone Marrow ◽  
Inflammatory Protein ◽  
Life Cycle Stages ◽  
Cytokine Levels ◽  
Necrosis Factor

Abstract Background The innate immune response against various life cycle stages of the malaria parasite plays an important role in protection against the disease and regulation of its severity. Phagocytosis of asexual erythrocytic stages is well documented, but little and contrasting results are available about phagocytic clearance of sexual stages, the gametocytes, which are responsible for the transmission of the parasites from humans to mosquitoes. Similarly, activation of host macrophages by gametocytes has not yet been carefully addressed. Methods Phagocytosis of early or late Plasmodium falciparum gametocytes was evaluated through methanol fixed cytospin preparations of immortalized mouse C57Bl/6 bone marrow-derived macrophages treated for 2 h with P. falciparum and stained with Giemsa, and it was confirmed through a standardized bioluminescent method using the transgenic P. falciparum 3D7elo1-pfs16-CBG99 strain. Activation was evaluated by measuring nitric oxide or cytokine levels in the supernatants of immortalized mouse C57Bl/6 bone marrow-derived macrophages treated with early or late gametocytes. Results The results showed that murine bone marrow-derived macrophages can phagocytose both early and late gametocytes, but only the latter were able to induce the production of inflammatory mediators, specifically nitric oxide and the cytokines tumour necrosis factor and macrophage inflammatory protein 2. Conclusions These results support the hypothesis that developing gametocytes interact in different ways with innate immune cells of the host. Moreover, the present study proposes that early and late gametocytes act differently as targets for innate immune responses.

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