scholarly journals Role of DNA-dependent protein kinase in recognition of radiation-induced DNA damage in human peripheral blood mononuclear cells

2001 ◽  
Vol 13 (6) ◽  
pp. 791-797 ◽  
Author(s):  
Daniela Frasca ◽  
Paola Barattini ◽  
Grazia Tocchi ◽  
Luisa Guidi ◽  
Luca Pierelli ◽  
...  
Keyword(s):  
Dna Damage ◽  
Protein Kinase ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Dependent Protein Kinase ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Dependent Protein ◽  
Radiation Induced

scholarly journals Oxidative Stress-Induced DNA Damage and Repair in Human Peripheral Blood Mononuclear Cells: Protective Role of Hemoglobin

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e68341 ◽  
Author(s):  
Anat Gafter-Gvili ◽  
Boris Zingerman ◽  
Benaya Rozen-Zvi ◽  
Yaacov Ori ◽  
Hefziba Green ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Peripheral Blood Mononuclear Cells ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Protective Role ◽  
Peripheral Blood Mononuclear ◽  
Dna Damage And Repair ◽  
Blood Mononuclear Cells

scholarly journals Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition

Cancers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 3843
Author(s):  
Jeffrey R. Whiteaker ◽  
Tao Wang ◽  
Lei Zhao ◽  
Regine M. Schoenherr ◽  
Jacob J. Kennedy ◽  
...  
Keyword(s):  
Dna Damage ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Limit Of Quantification ◽  
Peripheral Blood Mononuclear ◽  
Atm Kinase ◽  
Support Mechanism ◽  
Radiation Induced ◽  
Pharmacodynamic Biomarkers ◽  
Preclinical And Clinical Studies

The ATM serine/threonine kinase (HGNC: ATM) is involved in initiation of repair of DNA double-stranded breaks, and ATM inhibitors are currently being tested as anti-cancer agents in clinical trials, where pharmacodynamic (PD) assays are crucial to help guide dose and scheduling and support mechanism of action studies. To identify and quantify PD biomarkers of ATM inhibition, we developed and analytically validated a 51-plex assay (DDR-2) quantifying protein expression and DNA damage-responsive phosphorylation. The median lower limit of quantification was 1.28 fmol, the linear range was over 3 orders of magnitude, the median inter-assay variability was 11% CV, and 86% of peptides were stable for storage prior to analysis. Use of the assay was demonstrated to quantify signaling following ionizing radiation-induced DNA damage in both immortalized lymphoblast cell lines and primary human peripheral blood mononuclear cells, identifying PD biomarkers for ATM inhibition to support preclinical and clinical studies.

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