Collaborative Study of the Extraction of Plant Sterols from Adulterated Butter Oil Using a Digitonin-impregnated Celite Column

1970 ◽  
Vol 53 (3) ◽  
pp. 535-538
Author(s):  
Denis E Lacroix
Keyword(s):  
Liquid Chromatography ◽  
Vegetable Oil ◽  
Coefficient Of Variation ◽  
Collaborative Study ◽  
Screening Method ◽  
Rapid Screening ◽  
Gas Liquid Chromatography ◽  
Butter Oil ◽  
Average Coefficient ◽  
Rapid Screening Method

Abstract A rapid screening method for the analysis of the phytosterol, β-sitosterol, in butter oil adulterated with vegetable oil has been studied collaboratively. The sterols are removed from the adulterated butter oil by passing the sample through a digitonin-impregnated Celite 545 column, eluting the sterols with dimethyl sulfoxide, and analyzing the eluate for β-sitosterol by gas-liquid chromatography using a 3% JXR column. The average coefficient of variation for those samples containing more than 4 mg β-sitosterol/100 g adulterated butter oil is 12.6%. Therefore, β-sitosterol can be used as an index to qualitatively detect vegetable oil adulteration of butter oil.

Levels of Brominated Vegetable Oils in Soft Drinks by X-Ray Fluorescence Spectrometry and Gas-Liquid Chromatography

1970 ◽  
Vol 53 (3) ◽  
pp. 571-575
Author(s):  
H B S Conacher ◽  
J C Meranger ◽  
J Leroux
Keyword(s):  
Vegetable Oils ◽  
Screening Method ◽  
Soft Drinks ◽  
Fluorescence Spectrometry ◽  
Rapid Screening ◽  
Gas Liquid Chromatography ◽  
X Ray ◽  
Wide Range ◽  
Rapid Screening Method

Abstract A rapid screening method using X-ray fluorescence spectrometry has been developed for the detection and semiquantitative estimation of brominated vegetable oils in soft drinks. This method and a quantitative GLC technique have been applied to the determination of the brominated oil content in a wide range of soft drinks. Of 46 drinks examined, 23 contained brominated vegetable oils at levels between 7 and 85 mg/10 fluid oz of drink.

Gas-Liquid Chromatographic Screening Method for Determination of Methyl Mercury in Tuna and Swordfish

1983 ◽  
Vol 66 (1) ◽  
pp. 128-129
Author(s):  
Theron James
Keyword(s):  
Methyl Mercury ◽  
Methylene Chloride ◽  
Screening Method ◽  
Fish Tissue ◽  
Rapid Screening ◽  
Gas Liquid Chromatography ◽  
Absorption Method ◽  
Rapid Screening Method ◽  
Liquid Chromatographic

Abstract A rapid screening method has been developed for determining methyl mercury in tuna and swordfish. Fish tissue is blended with acidic KBr solution to release methyl mercury, which is then extracted into methylene chloride. After cleanup by partitioning with cysteine, the methyl mercury is extracted into toluene and determined by gas-liquid chromatography. The proposed method compares favorably with the official AOAC atomic absorption method.

Rapid Screening Method for Chick Edema Factor in Fats and Fatty Acids: 1967 Collaborative Study

1968 ◽  
Vol 51 (4) ◽  
pp. 940-942
Author(s):  
G R Higginbotham ◽  
John Ress ◽  
David Firestone
Keyword(s):  
Sulfuric Acid ◽  
Petroleum Ether Extract ◽  
Collaborative Study ◽  
Screening Method ◽  
Rapid Screening ◽  
Sulfuric Acid Treatment ◽  
Edema Factor ◽  
Capture Method ◽  
Rapid Screening Method ◽  
Chromatographic Peaks

Abstract A recently proposed improved rapid screening method for chick edema factor in fats was studied collaboratively by ten laboratories. The new procedure, a modification of the official electron capture method, reduces sample cleanup time by replacing the saponification step of the original procedure with a preliminary sulfuric acid cleanup step. Subsequent cleanup involves alumina column chromatography of the petroleum ether extract from sulfuric acid treatment, and gas chromatographic analysis at 200 °C of a specific polar alumina fraction. Gas chromatographic peaks with retention times vs. aldrin between 10 and 25 indicate the presence of chick edema factor. It is recommended that the modified procedure be adopted as official, first action.

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