Rapid Thin Layer Chromatographic Method for the Detection of Histamine in Fish Products

1976 ◽  
Vol 59 (6) ◽  
pp. 1224-1225 ◽  
Author(s):  
David E Schutz ◽  
George W Chang ◽  
Leonard F Bjeldanes
Keyword(s):  
Thin Layer ◽  
Chromatographic Separation ◽  
Chromatographic Method ◽  
Ammonium Hydroxide ◽  
Fish Products ◽  
Fish Flesh ◽  
Fish Samples ◽  
Tlc Plates

Abstract A rapid, convenient thin layer chromatographic (TLC) method for detecting histamine in fish samples is described. Samples of press juice or fish flesh are applied directly to TLC plates. The plates are developed with acetone-ammonium hydroxide (95+5) and the spots are visualized with ninhydrin or Pauly’s reagent. Chromatographic separation of histamine from other fish components is readily achieved by this method.

Rapid Thin Layer Chromatographic Method for Determining Aflatoxin Bi, Ochratoxin A, and Zearalenone in Corn

1978 ◽  
Vol 61 (3) ◽  
pp. 584-585
Author(s):  
Ivan Balzer ◽  
Čedo Bogdanić ◽  
Stjepan Pepeljnjak
Keyword(s):  
Sodium Bicarbonate ◽  
Thin Layer ◽  
Ochratoxin A ◽  
Ultraviolet Light ◽  
Aflatoxin B1 ◽  
Chromatographic Method ◽  
Limits Of Detection ◽  
Chloroform Extraction ◽  
Tlc Plates

Abstract A multimycotoxin thin layer chromatographic method is described for the analysis of corn. Aflatoxins are extracted from the samples with acetonitrile-water, and sodium bicarbonate is added to separate the acidic ochratoxin from zearalenone and aflatoxin B1. After chloroform extraction, 1/V NaOH is added to separate zearalenone and aflatoxin B1# The separated mycotoxins are spotted on TLC plates, which are then examined under ultraviolet light. The following recoveries ( % ) were obtained for corn samples: aflatoxin B1 71, ochratoxin A 87, and zearalenone 85. The limits of detection for the respective mycotoxins were 2, 40, and 200 ppb.

Thin Layer Chromatographic Separation and Spectrodensitometric Determination of Higher and Lower Sulfonated Subsidiary Dyes in FD&C Yellow No. 6

1975 ◽  
Vol 58 (4) ◽  
pp. 717-718
Author(s):  
Sandra Bell
Keyword(s):  
Thin Layer ◽  
Chromatographic Separation ◽  
Chromatographic Method ◽  
Disulfonic Acid

Abstract A thin layer chromatographic method is presented by which higher and lower sulfonated subsidiary dyes are simultaneously separated from FD&C Yellow No. 6. After separation, the colors are quantitated directly, using a spectrodensitometer. Recoveries of 1, 2, and 5% of added 1-(4-sulfophenylazo)-2-naphthol-3,6-disulfonic acid ranged from 92 to 111%. Recoveries of 1, 2, and 5% of added l-(4-sulfophenylazo)-2-naphthol ranged from 90 to 110%.

Thin Layer Chromatographic Method for Analysis and Chemical Confirmation of Sterigmatocystin in Cheese

1980 ◽  
Vol 63 (1) ◽  
pp. 110-114 ◽  
Author(s):  
Hans P Van Egmond ◽  
Walter E Paulsch ◽  
Ellen Deijll ◽  
Pieter L Schuller
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Trifluoroacetic Acid ◽  
Quantitative Method ◽  
Chromatographic Method ◽  
Aspergillus Versicolor ◽  
Spray Reagent ◽  
Tlc Plates ◽  
Layer Chromatography

Abstract A semi-quantitative method is described for the analysis of sterigmatocystin in cheese. The method is based on extraction of cheese with MeOH-4% KC1 (9+1), followed by Florisil and polyamide column cleanup and 2-dimensionaI thin layer chromatography (TLC). Visualization of sterigmatocystin on the TLC plates is enhanced by an ALCL3 spray reagent. The identity of sterigmatocystin is confirmed by a 2-dimensional TLC test based on reaction with trifluoroacetic acid (TFA) on the plate after first development. The reaction product formed is visualized by spraying with ALCL3. The method allows detection and confirmation of sterigmatocystin in cheese at concentrations as low as 5 μg/kg. The method has been applied to cheese samples ripening in warehouses and naturally molded with Aspergillus versicolor

Development of a Novel Thin-Layer Chromatographic Method of Screening the Red Beet (Beta vulgaris L.) Pigments in Alimentary Products

2020 ◽  
Vol 58 (1) ◽  
pp. 5-15 ◽  
Author(s):  
Eliza Łata ◽  
Agnieszka Fulczyk ◽  
Teresa Kowalska ◽  
Mieczysław Sajewicz
Keyword(s):  
Thin Layer ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Beetroot Juice ◽  
Commercial Sample ◽  
System A ◽  
Red Color ◽  
Butterfly Wings ◽  
Tlc Plates ◽  
Qualitative Confirmation

Abstract The aim of this study was to develop a thin-layer chromatographic method of qualitative analysis, aiming to confirm the presence of the red beetroot pigments in a given sample. The TLC system developed for this purpose consists of the precoated RP-18 F254s TLC plates and the acetonitrile + methanol + water + glacial acetic acid, 2:7:1:0.1 (v/v/v/v) mobile phase. With the use of this system, a striking horizontal separation of betacyanin pigments is obtained for both the red beetroot juice and the commercial betanin sample (with the left-to-right resolution distance of the two bands equal to ca. 6 mm), and a unique pattern of the two skewed chromatographic bands is observed. This striking phenomenon has been given a thorough consideration, and its tentative physicochemical justification was provided, based on analogical cases reported and extensively discussed in our earlier studies. Characteristic fingerprint obtained both for the beetroot juice and the commercial sample of betanin (resembling two slant butterfly wings) can prove very helpful for qualitative confirmation of the presence (or otherwise) of the betanin pigment in the red color juices and beverages, as it was demonstrated upon an example of elderberry juice with a confirmed fortification with the betanin pigment.

Improved Thin Layer Chromatographic Method for the Isolation and Estimation of Sterigmatocystin in Grains

1977 ◽  
Vol 60 (1) ◽  
pp. 104-106
Author(s):  
Albert K Athnasios ◽  
Gustav O Kuhn
Keyword(s):  
Acetic Acid ◽  
High Resolution ◽  
Thin Layer ◽  
Fluorescence Intensity ◽  
Chromatographic Method ◽  
Yellow Corn ◽  
Chcl3 Extract ◽  
Tlc Plates ◽  
Column Chromatographic ◽  
Initial Extraction

Abstract A modified thin layer chromatographic (TLC) method has been developed in conjunction with the method of Stack and Rodricks which effectively separates sterigmatocystin from interfering materials and eliminates the time-consuming column chromatographic cleanup step. Initial extraction with acetonitrile-water (9+1) containing KCl is followed by partition of the extract first against hexane and then against CHCl3. The CHCl3 extract is evaporated and the residue is dissolved in benzene and applied directly to pre-coated high resolution TLC plates, which are developed in benzene-acetic acid (90+10). Visualization of the sterigmatocystin on TLC plates is enhanced by an AlCl3 spray reagent, and concentrations of sterigmatocystin as low as 50 μg/kg can be estimated by comparing fluorescence intensity against a pure sterigmatocystin standard. The method has been applied to barley, white and yellow corn, oats, and wheat. Recoveries of added toxin in the range of 50—400 μg/kg ranged from 86.3% for yellow corn and oats to >96% for barley, wheat, and white corn.

Validated high-performance thin-layer chromatographic method for the determination of dibenzyl cyclooctadiene lignans fromSchisandra grandiflora

2014 ◽  
Vol 27 (6) ◽  
pp. 460-465 ◽  
Author(s):  
Katragunta Kumar ◽  
Bokka Ramesh ◽  
Vadaparthi Rao ◽  
Borra Poornima ◽  
Vanka Sarma ◽  
...  
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Chromatographic Method
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