Development of a Novel Thin-Layer Chromatographic Method of Screening the Red Beet (Beta vulgaris L.) Pigments in Alimentary Products

2020 ◽  
Vol 58 (1) ◽  
pp. 5-15 ◽  
Author(s):  
Eliza Łata ◽  
Agnieszka Fulczyk ◽  
Teresa Kowalska ◽  
Mieczysław Sajewicz
Keyword(s):  
Thin Layer ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Beetroot Juice ◽  
Commercial Sample ◽  
System A ◽  
Red Color ◽  
Butterfly Wings ◽  
Tlc Plates ◽  
Qualitative Confirmation

Abstract The aim of this study was to develop a thin-layer chromatographic method of qualitative analysis, aiming to confirm the presence of the red beetroot pigments in a given sample. The TLC system developed for this purpose consists of the precoated RP-18 F254s TLC plates and the acetonitrile + methanol + water + glacial acetic acid, 2:7:1:0.1 (v/v/v/v) mobile phase. With the use of this system, a striking horizontal separation of betacyanin pigments is obtained for both the red beetroot juice and the commercial betanin sample (with the left-to-right resolution distance of the two bands equal to ca. 6 mm), and a unique pattern of the two skewed chromatographic bands is observed. This striking phenomenon has been given a thorough consideration, and its tentative physicochemical justification was provided, based on analogical cases reported and extensively discussed in our earlier studies. Characteristic fingerprint obtained both for the beetroot juice and the commercial sample of betanin (resembling two slant butterfly wings) can prove very helpful for qualitative confirmation of the presence (or otherwise) of the betanin pigment in the red color juices and beverages, as it was demonstrated upon an example of elderberry juice with a confirmed fortification with the betanin pigment.

Rapid Thin Layer Chromatographic Method for Determining Aflatoxin Bi, Ochratoxin A, and Zearalenone in Corn

1978 ◽  
Vol 61 (3) ◽  
pp. 584-585
Author(s):  
Ivan Balzer ◽  
Čedo Bogdanić ◽  
Stjepan Pepeljnjak
Keyword(s):  
Sodium Bicarbonate ◽  
Thin Layer ◽  
Ochratoxin A ◽  
Ultraviolet Light ◽  
Aflatoxin B1 ◽  
Chromatographic Method ◽  
Limits Of Detection ◽  
Chloroform Extraction ◽  
Tlc Plates

Abstract A multimycotoxin thin layer chromatographic method is described for the analysis of corn. Aflatoxins are extracted from the samples with acetonitrile-water, and sodium bicarbonate is added to separate the acidic ochratoxin from zearalenone and aflatoxin B1. After chloroform extraction, 1/V NaOH is added to separate zearalenone and aflatoxin B1# The separated mycotoxins are spotted on TLC plates, which are then examined under ultraviolet light. The following recoveries ( % ) were obtained for corn samples: aflatoxin B1 71, ochratoxin A 87, and zearalenone 85. The limits of detection for the respective mycotoxins were 2, 40, and 200 ppb.

scholarly journals Validated HPTLC Method for Quantification of Luteolin and Apigenin inPremna mucronataRoxb., Verbenaceae

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Nayan G. Patel ◽  
Kalpana G. Patel ◽  
Kirti V. Patel ◽  
Tejal R. Gandhi
Keyword(s):  
Thin Layer ◽  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Method ◽  
Methanolic Extract ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Reflection Mode ◽  
Limit Of Quantitation ◽  
Hptlc Method

A simple, rapid, and precise high-performance thin-layer chromatographic method was developed for quantitative estimation of luteolin and apigenin inPremna mucronataRoxb., family Verbenaceae. Separation was performed on silica gel 60 F254HPTLC plates using toluene : ethyl acetate : formic acid (6 : 4 : 0.3) as mobile phase for elution of markers from extract. The determination was carried out in fluorescence mode using densitometric absorbance-reflection mode at 366 nm for both luteolin and apigenin. The methanolic extract ofPremna mucronatawas found to contain 10.2 mg/g % luteolin and 0.165 mg/g % of apigenin. The method was validated in terms of linearity, LOD and LOQ, accuracy, precision, and specificity. The calibration curve was found to be linear between 200 and 1000 ng/band for luteolin and 50 and 250 ng/band for apigenin. For luteolin and apigenin, the limit of detection was found to be 42.6 ng/band and 7.97 ng/band while the limit of quantitation was found to be 129.08 ng/band and 24.155 ng/band, respectively. This developed validated method is capable of quantifying and resolving luteolin and apigenin and can be applicable for routine analysis of extract and plant as a whole.

scholarly journals Determination of New Antidepressants in Pharmaceuticals by Thin-Layer Chromatography with Densitometry

2010 ◽  
Vol 93 (3) ◽  
pp. 778-782 ◽  
Author(s):  
Tatána Gondová ◽  
Iveta Petríková
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Pharmaceutical Formulations ◽  
Simultaneous Separation ◽  
Tlc Plates ◽  
Precision And Accuracy ◽  
Layer Chromatography

Abstract A new and simple TLC-densitometry method has been developed for the simultaneous separation of the two noradrenergic and specific serotonergic antidepressants mirtazapine and mianserine and validated for their determination in commercially available tablets. The method used TLC plates precoated with silica gel 60F254 as the stationary phase, and the mobile phase consisted of hexaneisopropanol25 ammonia (70 + 25 + 5, v/v/v). Densitometric analysis was carried out in the absorbance mode at 280 nm. The method was validated in accordance with International Conference on Harmonization guidelines in terms of linearity, LOD, LOQ, precision, and accuracy. Calibration curves were linear (R2 > 0.9970) with respect to peak area in the concentration range of 5002500 and 5005000 ng/spot for mirtazapine and mianserine, respectively. The LODs were 20 and 35 ng/spot for mirtazapine and mianserine, respectively. The described method was successfully applied to the determination of mirtazapine and mianserine in their pharmaceutical formulations with recovery ranging from 99.83 to 101.20 of the labeled amount of the compounds. The proposed method can be used in routine QC of these drugs in pharmaceutical formulations.

scholarly journals Some Observations on Thin Layer Chromatography Technique

2019 ◽  
Vol 8 (2) ◽  
pp. 1700-1702
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Potassium Permanganate ◽  
Mobile Phase ◽  
Technical Aspects ◽  
Potassium Permanganate Solution ◽  
Tlc Plates ◽  
Solvent Use ◽  
Layer Chromatography

The present study is to report the various problems faced during TLC methodology. Although used regularly some technical aspects must be kept in mind to get better and uniform results. During our experiments with TLC methods we came across some problems and here these aspects of TLC methodology are being highlighted. It is suggested that the solvent use as mobile phase should also be used for extraction of any particular phytochemical. TLC plates should be 3 to 4 mm thick, to be dried for at least 72 hrs. It is also suggested that potassium permanganate solution gives better clarity in visualizing the spots

scholarly journals A Rapid Thin Layer Chromatographic Method for Simultaneous Screening of Albendazole and Ivermectin in Formulations Prescribed for Human or Veterinary Use

2019 ◽  
Vol 31 (4) ◽  
pp. 896-900
Author(s):  
R.P. Pawar ◽  
P. Mishra ◽  
A. Durgbanshi ◽  
D. Bose
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Pharmaceutical Preparations ◽  
Cost Effective ◽  
Dosage Forms ◽  
Thin Layer Chromatographic Plate ◽  
Rf Values ◽  
Chromatographic Plate

An easy and selective thin layer chromatographic method has been developed and experimentally validated for the simultaneous screening of most commonly used anthelmintic drugs i.e. albendazole and ivermectin. Separation of these compounds was attained on silica gel 60 F254 pre-coated thin layer chromatographic plate using an optimized mobile phase of diisopropyl ether:ethyl acetate:glacial acetic acid in the ratio of 7:3:0.1 (v/v), respectively at pH 3.5. The calculated Rf values for albendazole and ivermectin were 0.65 and 0.38, respectively and the LOD was found to be 25 μg/ml and 30 μg/ml for albendazole and ivermectin, respectively. The developed method is selective, sensitive, robust, cost effective, eco-friendly, rapid as well as easy to perform. The developed method was successfully applied for the analysis of albendazole and ivermectin in pharmaceutical preparations marketed as oral suspensions, powder, tablets and injectable of the single or combined dosage forms for human as well as veterinary use. It could also be applied for the simultaneous analysis of both the compounds in other samples.

Rapid Thin Layer Chromatographic Method for the Detection of Histamine in Fish Products

1976 ◽  
Vol 59 (6) ◽  
pp. 1224-1225 ◽  
Author(s):  
David E Schutz ◽  
George W Chang ◽  
Leonard F Bjeldanes
Keyword(s):  
Thin Layer ◽  
Chromatographic Separation ◽  
Chromatographic Method ◽  
Ammonium Hydroxide ◽  
Fish Products ◽  
Fish Flesh ◽  
Fish Samples ◽  
Tlc Plates

Abstract A rapid, convenient thin layer chromatographic (TLC) method for detecting histamine in fish samples is described. Samples of press juice or fish flesh are applied directly to TLC plates. The plates are developed with acetone-ammonium hydroxide (95+5) and the spots are visualized with ninhydrin or Pauly’s reagent. Chromatographic separation of histamine from other fish components is readily achieved by this method.

scholarly journals Simultaneous analysis of eprosartan and hydrochlorothiazide in tablet formulation by High- Performance thin layer chromatography with ultraviolet absorption densitometry

2021 ◽  
Vol 8 (3) ◽  
pp. 123-128
Author(s):  
Vrushali Tambe ◽  
Vijaya Vichare ◽  
Anupa Wagh ◽  
Surekha Wavhal
Keyword(s):  
Thin Layer ◽  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Method ◽  
Glacial Acetic Acid ◽  
Tablet Formulation ◽  
Simultaneous Analysis ◽  
Phase Detection ◽  
Retention Factors ◽  
Layer Chromatography

A new, simple, accurate, and precise high-performance thin-layer chromatographic method has been established for simultaneous analysis of Eprosartan and Hydrochlorothiazide from a tablet formulation. Standard and sample solutions of Eprosartan and Hydrochlorothiazide were applied to precoated 250 μm layer of silica gel G 60 F and the plates were developed with Chloroform: Acetonitrile: Glacial Acetic Acid (7:3:1,v/v/v) as mobile phase. Detection and evaluation of densitograms was performed densitometrically at 254 nm. The linear range was 200-700 ng/band with the retention factors of Eprosartan and Hydrochlorothiazide were 0.26± 0.02 and 0.44±0.02, respectively. The method was validated and successfully used for analysis of the drugs in tablets.

scholarly journals Simultaneous Identification and Quantitative Determination of Selected Aminoglycoside Antibiotics by Thin-Layer Chromatography and Densitometry

2009 ◽  
Vol 92 (4) ◽  
pp. 1068-1075 ◽  
Author(s):  
Urszula Hubicka ◽  
Jan Krzek ◽  
Hanna Woltyska ◽  
Boóena Stachacz
Keyword(s):  
Thin Layer ◽  
Quantitative Determination ◽  
Mobile Phase ◽  
High Sensitivity ◽  
Good Separation ◽  
Fluorescent Indicator ◽  
Tlc Plates ◽  
Simultaneous Identification ◽  
Layer Chromatography

Abstract A TLCdensitometric method has been developed for simultaneous identification and quantitative determination of amikacin, gentamicin, kanamycin, neomycin, netilmicin, and tobramycin. This separation of antibiotics was achieved on silica gel TLC plates without a fluorescent indicator and with methanol25 ammoniachloroform (3 + 2 + 1, v/v/v) as the mobile phase. The densitometric measurements were made at 500 nm after detection with a 0.2 ninhydrin solution in ethanol. Under these conditions, good separation of the chosen aminoglycosides was obtained. The method is distinguished by high sensitivity, with the LOD from 0.25 g for amikacin to 1.00 g for gentamicin and the LOQ from 0.5 g for amikacin to 1.65 g for gentamicin, and a wide linearity range 0.756.25 g/spot for amikacin and netilmicin and 1.512.50 g/spot for other antibiotics. The precision of the determination was very good; RSD varied in the range 0.30.6.

scholarly journals Identification and Quantification of Germacrene D from the Extracts of Zanthoxylum ovalifolium Leaves by AgNO3-TLC Densitometric Method

2019 ◽  
Vol 31 (8) ◽  
pp. 1649-1652
Author(s):  
B.S. Theerthavathy ◽  
Shaukath Ara Khanum ◽  
S. Ravi Kiran
Keyword(s):  
Linear Regression ◽  
Thin Layer ◽  
Linear Relationship ◽  
Correlation Coefficient ◽  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Method ◽  
Analysis Data ◽  
Germacrene D ◽  
Simultaneous Identification

Germacrene D an important sesquiterpenoid has been extracted from leaves of Zanthoxylum ovalifoilum and high performance thin-layer chromatographic method has been developed for its simultaneous identification and determination. The compound was extracted by cold extraction and separated and identified on a AgNO3 impregnated silica gel 60 F254 HPTLC plates with dichloromethane:acetone (9.8:0.2 v/v) as mobile phase. The quantification of geremcare D was carried out by densitometry scanning performed at λ = 254 nm by reflectance scanning. This facilitated a well-resolved band for the compound with a Rf value of 0.46 ± 0.01. Linear regression concentration analysis data for mean calibration plots showed good linear relationship in the range of 1000-10000 ng/mL, with a correlation coefficient, slope and intercept of 0.9985 ± 0.0004, 21.62 ± 0.435 and 3308.87 ± 10.24, respectively. The minimum amount of germacrene D that could be authentically detected (LOD) and quantified (LOQ) were determined and found to be 212.07 and 521.23 ng/spot, respectively. The method was validated by considering the parameters linearity, precision, accuracy, specificity and robustness wherein, it is evident from the results that the proposed method was simple, safe and reliable.

Thin Layer Chromatographic Method for Analysis and Chemical Confirmation of Sterigmatocystin in Cheese

1980 ◽  
Vol 63 (1) ◽  
pp. 110-114 ◽  
Author(s):  
Hans P Van Egmond ◽  
Walter E Paulsch ◽  
Ellen Deijll ◽  
Pieter L Schuller
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Trifluoroacetic Acid ◽  
Quantitative Method ◽  
Chromatographic Method ◽  
Aspergillus Versicolor ◽  
Spray Reagent ◽  
Tlc Plates ◽  
Layer Chromatography

Abstract A semi-quantitative method is described for the analysis of sterigmatocystin in cheese. The method is based on extraction of cheese with MeOH-4% KC1 (9+1), followed by Florisil and polyamide column cleanup and 2-dimensionaI thin layer chromatography (TLC). Visualization of sterigmatocystin on the TLC plates is enhanced by an ALCL3 spray reagent. The identity of sterigmatocystin is confirmed by a 2-dimensional TLC test based on reaction with trifluoroacetic acid (TFA) on the plate after first development. The reaction product formed is visualized by spraying with ALCL3. The method allows detection and confirmation of sterigmatocystin in cheese at concentrations as low as 5 μg/kg. The method has been applied to cheese samples ripening in warehouses and naturally molded with Aspergillus versicolor

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