International Mycotoxin Check Sample Program. Part I. Report on the Performance of Participating Laboratories

1980 ◽  
Vol 63 (5) ◽  
pp. 1057-1066
Author(s):  
Marlin D Friesen ◽  
Ernest A Walker ◽  
Marcel Castegnaro
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Peanut Butter ◽  
Peanut Meal ◽  
Check Sample ◽  
Corn Meal ◽  
Sufficient Data ◽  
Meal Sample ◽  
Statistical Comparison ◽  
Significant Difference

Abstract Three aflatoxin-contaminated samples, raw peanut meal, finished peanut butter, and white corn meal, were analyzed by 139 laboratories in 34 countries. Sufficient data were obtained to permit a statistical comparison of the performance of laboratories using the BF, CB, and Pons methods and those using high performance liquid chromatography for quantification. A raw peanut meal sample showed no significant differences among means for laboratories using the four methods, and a white corn meal sample showed only one such significant difference; however, a finished peanut butter sample containing less than 10 μg total aflatoxins/kg showed 10 significant differences among means for laboratories using the 4 methods considered

International Mycotoxin Check Sample Program: Part I. Report on Laboratory Performance for Determination of Aflatoxins B1, B2, G1, and G2 in Raw Peanut Meal, Deoiled Peanut Meal, and Yellow Corn Meal

1982 ◽  
Vol 65 (4) ◽  
pp. 855-863
Author(s):  
Marlin D Friesen ◽  
Liliane Garren
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Peanut Meal ◽  
Check Sample ◽  
Corn Meal ◽  
Sufficient Data ◽  
Statistical Comparison ◽  
Yellow Corn

Abstract Three aflatoxin-contaminated samples (raw peanut meal, deoiled peanut meal, and yellow corn meal) were analyzed by 121 laboratories in 31 countries. Sufficient data were obtained to permit a statistical comparison of the performance of laboratories using the BF, CB, and EEC methods and those using high performance liquid chromatography (HPLC) for quantitation. No significant differences were found between means for laboratories using these 4 methods for the analysis of raw peanut meal or yellow corn meal. However, for deoiled peanut meal, means were significantly different for laboratories using the BF method compared with the CB or EEC methods for B1 and B2, and for laboratories using the CB method compared with HPLC methods for G2.

International Mycotoxin Check Sample Program: Part II. Report on Laboratory Performance for Determination of Aflatoxin M1 in Milk

1982 ◽  
Vol 65 (4) ◽  
pp. 864-868
Author(s):  
Marlin D Friesen ◽  
Liliane Garren
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Aflatoxin M1 ◽  
Check Sample ◽  
Sufficient Data ◽  
Statistical Comparison ◽  
Significant Difference ◽  
Aoac Method

Abstract A sample of aflatoxin M1-contaminated lyophilized cow's milk was analyzed by 80 laboratories in 30 countries. Sufficient data were obtained to permit a statistical comparison of the performance of laboratories using AOAC methods I and II and those using high performance liquid chromatography for quantitation. A significant difference was noted between means for laboratories using AOAC method I as opposed to those using HPLC methods. Overall reproducibility (between- plus within-laboratory precision) was best for laboratories using HPLC methods and poorest for those using AOAC method II.

Analyst Performance with Aflatoxin Methods as Determined from AOCS Smalley Check Sample Program: Short-Term and Long-Term Views

1984 ◽  
Vol 67 (1) ◽  
pp. 25-32
Author(s):  
John D Mckinney
Keyword(s):  
Collaborative Study ◽  
Peanut Meal ◽  
Extended Period ◽  
Positive Trend ◽  
Check Sample ◽  
Corn Meal ◽  
The Past ◽  
Sample Series ◽  
Significant Difference ◽  
Analytical Accuracy

Abstract The International Smalley Aflatoxin Check Sample Program of the American Oil Chemists' Society has offered check sample series for aflatoxins in peanut meal, cottonseed meal, and corn meal since 1976, and an aflatoxin M in raw milk series since 1980. This paper provides the computed mean of all analysts' results and between-laboratory precision for each of the samples in each of the check sample series distributed in 1980–81 and 1981–82. In addition, a comparison is made of the relative measurement and analytical accuracy of those analysts who have participated in the peanut meal series for at least 4 years and in the cottonseed and corn meal series since their inception (6 years). For this comparison, each analyst's result for each sample was calculated as a percent of the mean for all analysts for that sample; these values were then averaged for each analyst over all the meal samples in all the series for each meal type in which the analyst had participated, to obtain an overall measure of analytical accuracy. A similar calculation was made using the reported results for the defined solution of anatoxins included in each series, to obtain an overall measure of measurement accuracy. An evaluation of the meal series results for the past 2 seasons shows an overall within-laboratory precision in the range reported for the collaborative studies by which the methods were validated; the between-laboratory precision, although improved over past years, is still far from the collaborative study range. The precision data for the aflatoxin solution included in each series indicate this bias could be related, in large part, to the reference standards used. The extended period evaluation of analysts' performance shows no apparent correlation between measurement and analytical accuracy except for a general positive trend for those analysts using the BF method for anatoxins in peanut meal. A comparison of the accuracy of the BF and CB methods for aflatoxins in peanut meal shows no significant difference between results by the 2 methods on the basis of the extended period evaluation, in contrast to the generally higher results for the CB method in the past 2 seasons' evaluation. The scatter in the average analysts' analytical accuracy is essentially the same, regardless of the method used.

International Aflatoxin Check Sample Program: 1972 Study

1973 ◽  
Vol 56 (2) ◽  
pp. 322-327
Author(s):  
Francis B Coon ◽  
Fred J Baur ◽  
L Richardson L Symmes
Keyword(s):  
United States ◽  
Peanut Butter ◽  
The United States ◽  
Single Sample ◽  
Check Sample ◽  
Sufficient Data ◽  
Mean Values ◽  
Statistical Comparison ◽  
Sample Series ◽  
The World

Abstract In the second International Aflatoxin Check Sample Series, a single sample, peanut butter, was submitted to 152 laboratories throughout the world. Sufficient data were obtained from 117 responding laboratories to permit a statistical comparison of the BF, CB, and Pons methods. No significant differences in mean values were obtained. One quarter of the participating laboratories were from countries other than Canada and the United States.

scholarly journals Influence of spray mixture volume and flight height on herbicide deposition in aerial applications on pastures

2014 ◽  
Vol 32 (1) ◽  
pp. 227-232 ◽  
Author(s):  
M.A.P. Oliveira ◽  
U.R. Antuniassi ◽  
E.D. Velini ◽  
R.B. Oliveira ◽  
J.F. Salvador ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Mineral Oil ◽  
Mato Grosso ◽  
Significant Difference ◽  
Flight Height ◽  
Mixture Volume ◽  
Pasture Area ◽  
Experimental Plots

The objective of the present study was to analyze the influence of spray mixture volume and flight height on herbicide deposition in aerial applications on pastures. The experimental plots were arranged in a pasture area in the district of Porto Esperidião (Mato Grosso, Brazil). In all of the treatments, the applications contained the herbicides aminopyralid and fluroxypyr (Dominum) at the dose of 2.5 L c.p. ha-1, including the adjuvant mineral oil (Joint Oil) at the dose of 1.0 L and a tracer to determine the deposition by high-performance liquid chromatography (HPLC) (rhodamine at a concentration of 0.6%). The experiment consisted of nine treatments that comprised the combinations of three spray volumes (20, 30 and 50 L ha-1) and three flight heights (10, 30 and 40 m). The results showed that, on average, there was a tendency for larger deposits for the smallest flight heights, with a significant difference between the heights of 10 and 40 m. There was no significant difference among the deposits obtained with the different spray mixture volumes.

scholarly journals Analysis of the High-Performance Liquid Chromatography Fingerprints and Quantitative Analysis of Multicomponents by Single Marker of Products of Fermented Cordyceps sinensis

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Li-hua Chen ◽  
Yao Wu ◽  
Yong-mei Guan ◽  
Chen Jin ◽  
Wei-feng Zhu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Performance ◽  
Cordyceps Sinensis ◽  
Array Detector ◽  
Hplc Fingerprint ◽  
External Standard Method ◽  
Significant Difference ◽  
Single Marker

Fermented Cordyceps sinensis, the succedaneum of Cordyceps sinensis which is extracted and separated from Cordyceps sinensis by artificial fermentation, is commonly used in eastern Asia in clinical treatments due to its health benefit. In this paper, a new strategy for differentiating and comprehensively evaluating the quality of products of fermented Cordyceps sinensis has been established, based on high-performance liquid chromatography (HPLC) fingerprint analysis combined with similar analysis (SA), hierarchical cluster analysis (HCA), and the quantitative analysis of multicomponents by single marker (QAMS). Ten common peaks were collected and analysed using SA, HCA, and QAMS. These methods indicated that 30 fermented Cordyceps sinensis samples could be categorized into two groups by HCA. Five peaks were identified as uracil, uridine, adenine, guanosine, and adenosine, and according to the results from the diode array detector, which can be used to confirm peak purity, the purities of these compounds were greater than 990. Adenosine was chosen as the internal reference substance. The relative correction factors (RCF) between adenosine and the other four nucleosides were calculated and investigated using the QAMS method. Meanwhile, the accuracy of the QAMS method was confirmed by comparing the results of that method with those of an external standard method with cosines of the angles between the groups. No significant difference between the two methods was observed. In conclusion, the method established herein was efficient, successful in identifying the products of fermented Cordyceps sinensis, and scientifically valid to be applicable in the systematic quality control of fermented Cordyceps sinensis products.

scholarly journals Determination of haemoglobin A1c levels using high-performance liquid chromatography of bloodstains

2019 ◽  
Vol 60 (1) ◽  
pp. 19-25
Author(s):  
Kemalettin Acar ◽  
Ayse Kurtulus Dereli ◽  
Esin Avci ◽  
Volkan Zeybek ◽  
Erdi Kutlu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
High Reliability ◽  
Receiver Operating Curve ◽  
Haemoglobin A1c ◽  
Receiver Operating Curve Analysis ◽  
Significant Difference ◽  
Glass Surfaces ◽  
Hba1c Levels

This study aimed to determine haemoglobin A1c (HbA1c) levels in bloodstains shed on glass and fabric surfaces on specified test dates. Blood samples were taken from 26 patients (13 diabetic and 13 non-diabetic). Initial HbA1c levels were detected by using high-performance liquid chromatography (HPLC), and bloodstains were created on both cotton fabric and glass surfaces. Samples were processed at different ages (0, 7, 14, 28 and 56 days) by diluting distilled water and then measuring HbA1c levels by HPLC again. In all stains, HbA1c levels could be determined by using HPLC, but there was a moderate rise in accordance with the age of the stains. A statistically significant difference was found for bloodstains on clothes compared to those on glass surfaces. Receiver operating curve analysis found a sensitivity of 1.0 and specificity of 0.923 (cut-off 6.55) for glass surfaces on the seventh day; a sensitivity of 1.0, a specificity of 0.846 (cut-off 6.45) for clothes on the seventh day; a sensitivity of 1.0 and a specificity of 0.923 (cut-off 6.85) for clothes on the 56th day; and a sensitivity of 1.0 and a specificity of 0.846 (cut-off 7.55) for glass surfaces on the 56th day. In conclusion, this study found that HbA1c levels could be measured with high reliability from forensic bloodstains by using HPLC. Thus, in cases where DNA data banks cannot identify individuals, it would make sense to turn to those who have a medical history of diabetes among the suspects with the results of high HbA1c levels.

scholarly journals Investigation of eluted monomers from resin-based root canal sealer by high-performance liquid chromatography analysis

2016 ◽  
Vol 10 (01) ◽  
pp. 092-096 ◽  
Author(s):  
Huma Omurlu ◽  
Hacer Deniz Arisu ◽  
Evrim Eliguzeloglu Dalkilic ◽  
Ugur Tamer ◽  
Hilal Torul
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Bisphenol A ◽  
Root Canal ◽  
High Performance ◽  
Light Emitting Diode ◽  
Root Canal Sealer ◽  
Chromatography Analysis ◽  
Significant Difference ◽  
Time Periods

ABSTRACT Objective: The purpose of the current study was to determine the amount of urethane dimethacrylate (UDMA), bisphenol A-glycidyl methacrylate (Bis-GMA), poly (ethylene glycol) dimethacrylate (PEGDMA), bisphenol A ethoxylated dimethacrylate (Bis-EMA), and 2-hydroxyethyl methacrylate (HEMA) eluted from resin-based root canal sealer, epiphany, using high-performance liquid chromatography (HPLC). Materials and Methods: Epiphany was placed into the plastic molds and light-cured with a light emitting diode. After the curing process, each specimen in the first group (n = 12) was immersed in Eppendorf tubes containing a phosphate-buffered saline solution (PBS) and incubated for 45 s. In the second group, each specimen (n = 12) was immersed in Eppendorf tubes containing PBS and incubated for 24 h. Of the specimen extracts, 100 μL were subjected to HPLC. Analysis of data was accomplished with one-way analysis of variance (P < 0.05). Results: All of the samples eluted HEMA, UDMA, Bis-GMA, PEGDMA, and Bis-EMA. A significant difference was determined between the time periods of HEMA, UDMA, PEGDMA, and Bis-EMA (P < 0.05). Conclusion: The results of the current study showed that Epiphany releases HEMA, UDMA, Bis-GMA, PEGDMA, and Bis-EMA in both time periods.

scholarly journals EFFECT OF DIFFERENT ANTICOAGULANTS IN DETERMINING METFORMIN HYDROCHLORIDE LEVELS IN HUMAN PLASMA USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

2018 ◽  
Vol 10 (1) ◽  
pp. 148
Author(s):  
Yahdiana Harahap ◽  
Lista Roro Marsudi ◽  
Sunarsih .
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
High Performance ◽  
Ethylenediaminetetraacetic Acid ◽  
Sodium Dodecyl ◽  
Internal Standard ◽  
Array Detector ◽  
Metformin Hydrochloride ◽  
Significant Difference

Objective: This study aimed to develop and validate an analytical method and to determine the effect of different anticoagulants for analyzingmetformin HCl level in human plasma.Methods: Analysis was performed with high-performance liquid chromatography method using a photodiode array detector set at 234 nm, a C18SunFire™ column (5 μm, 250 mm×4.6 mm), a temperature of 40°C, a mobile phase comprising 10 mM sodium dodecyl sulfate and 10 mM phosphatebuffer in water-acetonitrile (60:40 v/v) at pH 7.0, with a flow rate of 1.0 mL/min, and atorvastatin calcium as the internal standard. Plasmaextraction was performed through protein precipitation using human plasma (300 μL) and acetonitrile (600 μL; 1:2 v/v). The method was linear at aconcentration range of 20.0–5000.0 ng/mL, with r>0.9998.Results: The stability and recovery of metformin HCl in human plasma were not different between citrate with ethylenediaminetetraacetic acid(EDTA) and heparin with EDTA as anticoagulants. However, a significant difference in the peak area response ratio (p<0.05) was observed betweenthe three anticoagulants.Conclusion: The validation results for the three types of plasma anticoagulants met validation requirements based on the European MedicinesAgency bioanalytical guidelines of 2011 for accuracy and precision, selectivity, calibration curve linearity, dilution integrity, carry-over, and stability.

International Aflatoxin Check Sample Program: 1971 Study

1972 ◽  
Vol 55 (2) ◽  
pp. 315-327
Author(s):  
F B Coon ◽  
F J Baur ◽  
L R L Symmes
Keyword(s):  
Aflatoxin Contamination ◽  
Check Sample ◽  
Sufficient Data ◽  
Statistical Comparison ◽  
The World

Abstract An International Aflatoxin Check Sample Committee was formed in 1971 to establish a check sample program of commodities or materials with aflatoxin contamination, which would be available to laboratories throughout the world. A set of 4 aflatoxin-contaminated peanut samples was prepared and mailed to 150 participating laboratories. Sufficient data were obtained from this study to permit a statistical comparison of 3 of the 4 AOAC methods for analysis of peanuts and peanut commodities. The analysis showed significantly higher means for the CB method tbun for the BF or Pons method for 2 of the samples examined.

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