Separation and Identification of Nine Penicillins by Reverse Phase Liquid Chromatography

1984 ◽  
Vol 67 (2) ◽  
pp. 228-231
Author(s):  
Gregory T Briguglio ◽  
◽  
Cesar A Lau-Cam
Keyword(s):  
High Performance ◽  
Potassium Dihydrogen Phosphate ◽  
High Performance Liquid Chromatographic ◽  
Penicillin G ◽  
Dihydrogen Phosphate ◽  
Potassium Dihydrogen ◽  
Liquid Chromatographic Method ◽  
Phase Liquid ◽  
Wavelength Detector ◽  
Liquid Chromatographic

Abstract A simple and rapid high performance liquid chromatographic method was developed for the separation and identification of amoxicillin, ampicillin, cloxacillin, dicloxacillin, methicillin, oxacillin, nafcillin, penicillin G potassium, and penicillin V potassium. The antibiotics were separated at ambient temperature on a Chromegabond 10 μm Cis column with acetonitrile-methanol-O.OlM potassium dihydrogen phosphate buffer, pH 4.7 (19 + 11 + 70), at 1 mL/min. A variable wavelength detector set at 225 nm, 0.16 AUFS, and a recorder set at 0.25 cm/min were used for the detection. Individual antibiotics and their mixtures were dissolved in the mobile phase and injected into the chromatograph through a 20 μL injection loop. Baseline separation was observed for virtually all 9 antibiotics. The entire mixture was resolved in less than 30 min. The method was sensitive, reproducible, and applicable to the qualitative analysis of commercial dosage forms.

scholarly journals Estimation of Lamotrigine by RP-HPLC Method

2010 ◽  
Vol 7 (s1) ◽  
pp. S203-S208
Author(s):  
D. Anantha Kumar ◽  
CH. Venkata Kumar ◽  
P. Seetharamaiah ◽  
J. V. L. N. Seshagiri Rao
Keyword(s):  
High Performance ◽  
Potassium Dihydrogen Phosphate ◽  
High Performance Liquid Chromatographic ◽  
Dosage Forms ◽  
Hplc Method ◽  
Dihydrogen Phosphate ◽  
Pharmaceutical Dosage Forms ◽  
Liquid Chromatographic Method ◽  
Tablet Dosage ◽  
Liquid Chromatographic

A rapid and reproducible reverse phase high performance liquid chromatographic method has been developed for the estimation of lamotrigine in its pure form as well as in pharmaceutical dosage forms. Chromatography was carried out on a Luna C18column using a mixture of potassium dihydrogen phosphate buffer (pH 7.3) and methanol in a ratio of 60:40 v/v as the mobile phase at a flow rate of 1.0 mL/min. The detection was done at 305 nm. The retention time of the drug was 6.1 min. The method produced linear responses in the concentration range of 10 to 70 μg/mL of lamotrigine. The method was found to be reproducible for analysis of the drug in tablet dosage forms.

DEVELOPMENT OF LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS DETERMINATION OF BROMHEXINE HYDROCHLORIDE AND ENROFLOXACIN IN FORMULATIONS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (02) ◽  
pp. 44-49
Author(s):  
P Choksi ◽  
◽  
F. Shaikh ◽  
D. A. Shah ◽  
K. Agarwal ◽  
...  
Keyword(s):  
Potassium Dihydrogen Phosphate ◽  
Fixed Dose ◽  
Dihydrogen Phosphate ◽  
Potassium Dihydrogen ◽  
Liquid Chromatographic Method ◽  
Dose Combination ◽  
Reproducible Method ◽  
Liquid Chromatographic ◽  
Isocratic Mode

A simple, specific, accurate, precise and reproducible method has been developed and validated for the estimation of bromhexine hydrochloride and enrofloxacin in fixed dose combination using RP-HPLC. The separation was achieved using stationary phase ODS Hypersil C18 column (250 mm× 4.6 mm i.d.) in isocratic mode, with mobile phase containing 0.05 M potassium dihydrogen phosphate buffer (pH 4 by o-phosphoric acid) : methanol: acetonitrile : triethylamine (40:20:40:01), at a flow rate of 1.0mL/min and eluents were monitored at 256 nm. The retention time of enrofloxacin and bromhexine HCl were found to be 3.00 min and 5.1 min respectively. The linearity for bromhexine HCl and enrofloxacin was in the range of 2-15 μg/mL and 20-150 μg/mL, respectively. The method was validated as per ICH guideline. The recoveries of bromhexine HCl and enrofloxacin were found in the range of 99.61-101.65% and 99.52-100.13 %, respectively. The method was successfully applied for the determination of both the drugs in combined dosage form.

scholarly journals Validated High-Throughput HPLC Method for the Analysis of Flavonol Aglycones Myricetin, Quercetin, and Kaempferol in Rhus coriaria L. Using a Monolithic Column

2009 ◽  
Vol 92 (4) ◽  
pp. 1035-1043 ◽  
Author(s):  
Morteza Mehrdad ◽  
Mahnoosh Zebardast ◽  
Ghazaleh Abedi ◽  
Mitra Nouri Koupaei ◽  
Hoda Rasouli ◽  
...  
Keyword(s):  
High Performance ◽  
Monolithic Column ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Hplc Method ◽  
Potassium Dihydrogen ◽  
Liquid Chromatographic Method ◽  
Rhus Coriaria ◽  
Liquid Chromatographic ◽  
Potassium Dihydrogen Orthophosphate

Abstract A rapid and simple reversed-phase high-performance liquid chromatographic method using a monolithic column was developed and validated for the separation and quantification of myricetin, quercetin, and kaempferol in Rhus coriaria L. The method employed the isocratic mobile phase acetonitrile10 mM potassium dihydrogen orthophosphate buffer adjusted to pH 3.0 using orthophosphoric acid (38 + 62, v/v) at a flow rate of 4.0 mL/min; a Chromolith Performance RP-18e (100 4.6 mm) monolithic column kept at 40C; and UV detection at 370 nm. Although the elution order was identical and the selectivity was equivalent, the comparison between monolithic and particulate columns showed that the monolithic column could reduce the separation time to <1 min without sacrificing column efficiency and selectivity. The method was validated according to International Conference on Harmonization guidelines. The validation characteristics included accuracy, precision, linearity, range, specificity, LOQ, and robustness. The calibration curves were linear (r >0.999) over the concentration range of 0.8888.3 g/mL for myricetin, 0.9595 g/mL for quercetin, and 1.43143.3 g/mL for kaempferol. The recoveries for all three compounds were above 89. Myricetin was found to be the major flavonol in the examined plant extracts, followed by minor quantities of quercetin and kaempferol.

scholarly journals THE ESTIMATION METHOD OF S-ACID LACTIC IN THE FERMENTATION PRODUCTS OF ALOE VERA GEL BY HIGH- PERFORMANCE LIQUID CHROMATOGRAPHY

2017 ◽  
Vol 126 (1B) ◽  
pp. 107
Author(s):  
Nguyen Kim Dong ◽  
Duong Thi Bich ◽  
Nguyen Chi Toan
Keyword(s):  
High Performance ◽  
Aloe Vera ◽  
Estimation Method ◽  
Potassium Dihydrogen Phosphate ◽  
High Performance Liquid Chromatographic ◽  
Dihydrogen Phosphate ◽  
Uv Detector ◽  
Fermentation Products ◽  
Aloe Vera Gel ◽  
Liquid Chromatographic

<p> <em>An accurate, precise, simple and economical High Performance Liquid Chromatographic method for the estimation of </em><em>S-acid lactic in the process of fermentation</em><em> of </em><em>Aloe Vera</em><em> gel</em><em> has been developed. The method is using </em><em>HIQSIL 100 C18column (Length: 250nm, Diameter: 4.6nm, Particle size: 5μ) with potassium dihydrogen phosphate as mobile phase. The separation was performed by UV detector at 210 nm</em> <em>wavelength. The flow rate was kept at 1mL/min and the injection volume was 20μl. The results obtained showed that the linearity of the method was 0-180 ppm, and the correlation coefficient was found to be 0.999. Detection limit of the method for S-acid lactic was 1.15 ppm. The mean recoveries were from 95% to 99%. The reproducibility RSDs ranged from 2.87%. The results demonstrated the suitability of the HPLC approach for the analysis of S-acid lactic in the process of fermentation from Aloe Vera gel.</em></p>

scholarly journals Development of a Stability-Indicating High-Performance Liquid Chromatographic Method for the Simultaneous Determination of Alprazolam and Sertraline in Combined Dosage Forms

2008 ◽  
Vol 91 (6) ◽  
pp. 1344-1353 ◽  
Author(s):  
Ashutosh Pathak ◽  
Sadhana J Rajput
Keyword(s):  
High Performance ◽  
Chromatographic Method ◽  
Degradation Products ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Dosage Forms ◽  
Dihydrogen Phosphate ◽  
Stability Indicating ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract The objective of the current study was to develop a validated stability-indicating high-performance liquid chromatographic method for alprazolam and sertraline in combined dosage forms. The method was validated by subjecting the drugs to forced decomposition under hydrolysis, oxidation, photolysis, and thermal stress conditions prescribed by the International Conference on Harmonization. The drugs were successfully separated from major and minor degradation products on a reversed-phase C18 column by using 75 mM potassium dihydrogen phosphate buffer (pH 4.3)acetonitrilemethanol (50 45 5, v/v/v) as the mobile phase with determination at 227 nm. The flow rate was 0.9 mL/min. The method was validated with respect to linearity, precision, accuracy, system suitability, and robustness. The responses were linear over the ranges of 180 and 5200 g/mL for alprazolam and sertraline, respectively. The recoveries of both drugs from a mixture of degradation products were in the range of 97101. The utility of the procedure was verified by its application to marketed formulations that were subjected to accelerated stability studies. The method distinctly separated the drugs and degradation products, even in actual samples. The products formed in marketed tablets were similar to those formed during stress studies.

scholarly journals High-Performance Liquid Chromatographic Method for the Determination of Moniliformin in Corn

1998 ◽  
Vol 81 (5) ◽  
pp. 999-1004 ◽  
Author(s):  
Célestin Munimbazi ◽  
Lloyd B Bullerman
Keyword(s):  
High Performance ◽  
Chromatographic Method ◽  
Solid Phase ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Detector Response ◽  
Dihydrogen Phosphate ◽  
Sodium Dihydrogen Phosphate ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A high-performance liquid chromatographic method using UV absorption was developed for determining moniliformin in corn. The toxin was extracted with water containing 1 % tetrabutylammonium hydrogen sulfate (w/v). Paired moniliformin was partitioned into dichloromethane, which was evaporated to dryness at 50 C. The residue was dissolved in water and applied to a disposable stronganion exchange solid-phase extraction tube. Adsorbed moniliformin was eluted from the tube with 0.05M sodium dihydrogen phosphate monohydrate (pH 5). It was determined by ion-pair reversed-phase chromatography and UV measurement at 229 nm. The minimum detectable amount of pure moniliformin was 0.25 ng/injection (signal-to-noise ratio = 3:1). The detector response was linear from 0.25 to at least 20 ng. The limit of determination was 0.025 μg/g corn. Recoveries of moniliformin from corn spiked at 0.025,0.05,0.25, and 1.0 μg/g averaged 96.5,96.2, 97.2, and 97.8% respectively

scholarly journals A Versatile Liquid Chromatographic Method for the Simultaneous Determination of Metformin, Sitagliptin, Simvastatin, and Ezetimibe in Different Dosage Forms

2018 ◽  
Vol 101 (2) ◽  
pp. 401-409 ◽  
Author(s):  
Asmaa A El-Zaher ◽  
Ehab F Elkady ◽  
Hanan M Elwy ◽  
Mahmoud Abo El Makarim Saleh
Keyword(s):  
Potassium Dihydrogen Phosphate ◽  
Reversed Phase ◽  
Pharmaceutical Products ◽  
Metformin Hydrochloride ◽  
Dihydrogen Phosphate ◽  
Potassium Dihydrogen ◽  
Accuracy And Precision ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A new LC method is introduced with the concept of its versatile application to widely used drugs from different pharmacological classes. Metformin hydrochloride (MTF), sitagliptin phosphate (SIT), simvastatin (SIM) and ezetimibe (EZB) were simultaneously determined with a simple reversed-phase LC method in which a SIT–SIM binary mixture, present in a dosage form brand, was considered central for its development. Chromatographic separation was achieved with a mobile phase of acetonitrile and 0.02 M potassium dihydrogen phosphate (pH 5.2) (77 + 23, v/v) flowing through a C18 column (BDS Hypersil, 250 × 4.6 mm, 5 µm) at 1.2 mL/min at ambient temperature. UV detection was programmed to be carried out at 210 nm for EZB, SIT, and MTF, whereas SIM was detected at 240 nm. The method was validated according to International Conference on Harmonization guidelines. Linearity, accuracy, and precision were satisfactory over concentration ranges 4–40 µg/mL for EZB and SIM, 0.5–50 µg/mL for SIT, and 5–500 µg/mL for MTF. Coefficients of determination were &gt;0.99 for the four drugs. LOQs found were 0.01 µg/mL for EZB, 0.02 µg/mL for SIT, 0.2 µg/mL for MTF, and 0.02 µg/mL for SIM. The developed method is simple, rapid, accurate, precise, and suitable for the routine QC analysis of the cited drugs in pharmaceutical products by conventional HPLC systems.

Development and Validation of a Stability-Indicating High-Performance Liquid Chromatographic Method for the Quantification of Methocarbamol and Its Impurities in Pharmaceutical Dosage Forms

2021 ◽  
Author(s):  
Shivani Kalokhe ◽  
Santaji Nalwade ◽  
Pallavi Patil ◽  
Poonam Raskar
Keyword(s):  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Dihydrogen Phosphate ◽  
Pharmaceutical Dosage Forms ◽  
Stability Indicating ◽  
Liquid Chromatographic Method ◽  
Validation Parameters ◽  
Limit Of Quantitation ◽  
Liquid Chromatographic

Abstract A novel, delicate, stability-indicating, gradient, reversed-phase high-performance liquid chromatographic method has been established for the quantitative estimation of methocarbamol (MTC) and its impurities present in a pharmaceutical oral suspension. XBridge C18, 5 μm, 250 mm × 4.6 mm column was used to accomplish chromatographic separation with a buffered mobile phase consisting of a mixture of 0.01 M of sodium dihydrogen phosphate (pH 7.0 buffer) and methanol in the ratio of 95:05 (v/v), respectively, were used as solvent A and a mixture of methanol and Milli-Q water in the ratio 90:10 (v/v), respectively, was used as solvent B. Analysis was carried out at 0.8 mL/min flow rate and the detection wavelength at 225 nm. The compartment temperature of the column is put at 25°C. The resolution of MTC and its four impurities has been attained &gt;2.0 for all pairs of compounds. Significant degradation of MTC was photolytic, thermal and oxidative stress conditions. Validation of the developed method was performed as stated by the International Conference on Harmonization guidelines with regard to all validation parameters like specificity, accuracy, linearity, precision, limit of detection, limit of quantitation and robustness.

scholarly journals Determination of Pantoprazole Sodium and Lansoprazole in Individual Tablet Dosage Forms by RP-HPLC Using Single Mobile Phase

2009 ◽  
Vol 6 (2) ◽  
pp. 489-494 ◽  
Author(s):  
B. Prasanna Kumar Reddy ◽  
Y. Ramanjaneya Reddy ◽  
D. Ramachandran
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Pharmaceutical Products ◽  
Liquid Chromatographic Method ◽  
Phase Liquid ◽  
Tablet Dosage ◽  
Liquid Chromatographic

A simple, sensitive and precise high performance liquid chromatographic method for the analysis of pantoprazole sodium and lansoprazole has been developed, validated and used for the determination of compounds in commercial pharmaceutical products. The compounds were well separated an isocratically on a C18column [Inertsil C18, 5μ, 150 mm x 4.6 mm] utilizing a mobile phase consisting of acetonitrile: phosphate buffer (60:40, v/v, pH 7.0) at a flow rate of 1.0 mL/min with UV detection at 230 nm. The retention time of pantoprazole sodium and lansoprazole was found to be 2.017 min and 2.538. The procedure was validated for linearity (Correlation coefficient=0.999). The study showed that reversed-phase liquid chromatography is sensitive and selective for the determination of pantoprazole sodium and lansoprazole using single mobile phase.

scholarly journals Simultaneous Quantification of Ciprofloxacin, Quinine and 3-hyrdoxyquinine in Human Plasma using a HPLC Method

2016 ◽  
Vol 8 (1) ◽  
pp. 11 ◽  
Author(s):  
Adebanjo J. Adegbola ◽  
Julius O. Soyinka
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Potassium Dihydrogen Phosphate ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Hplc Method ◽  
Therapeutic Monitoring ◽  
Dihydrogen Phosphate ◽  
Antimalarial Therapy ◽  
Liquid Chromatographic

Malaria has been shown to strongly predispose patients in areas of malaria endemicity to bacteremia with severe outcomes, thus justifying the use of antibiotics in combination with antimalarial therapy in patients with severe malaria. This study describes a High-Performance Liquid Chromatographic (HPLC) method for simultaneous determinations of Ciprofloxacin (CPN), Quinine (QN), and its major metabolite, 3-Hydroxyquinine (3-HQN), in human plasma. Following a simple precipitation with acetonitrile, chromatographic separation was achieved on a reversed-phase Agilent Zorbax (CN) column (5 μm, 150 X 4.6 mm i.d) using a mobile phase consisting of acetonitrile: potassium dihydrogen phosphate (pH = 2.8; 0.02 M) (42:58, v/v). Retention times for CPN, 3-HQN, IS and QN were 2.7, 3.3, 3.6 and 4.9 minutes respectively. The limits of detection and validated lower limits of quantitation were 30 and 70 ng/ml for both QN and 3-HQN while the corresponding values were 50 and 100 ng/ml for CPN, respectively. The new HPLC method here developed, when compared with previous methods for the analysis of either or both drugs is simple, rapid, selective, reproducible and costeffective. It is also suitable for conducting a simultaneous therapeutic monitoring of quinine and ciprofloxacin in patients when concomittantly administered as demonstrated in five healthy volunteers.

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