Rapid Quantitative Thin Layer Chromatographic Screening Procedure for Sulfathiazole Residues in Honey

1986 ◽  
Vol 69 (4) ◽  
pp. 641-643
Author(s):  
Eli Neidert ◽  
Zig Baraniak ◽  
André Sauvé

Abstract A procedure is presented for the quantitative determination of sulfathiazole residues in honey. Induced fluorescence of sulfathiazole is measured by fluorescent scanning densitometry; sulfaquinoxaline is added as an internal standard for quantitation. Recovery is greater than 98% and results are linear over the range 0.05-0.60 mg/kg. The detection limit, CL (k = 3), is 0.02. The procedure allows a single analyst to process 50-60 samples/day.

1978 ◽  
Vol 61 (6) ◽  
pp. 1415-1418
Author(s):  
Paul R Beljaars ◽  
Theo M M Rondags

Abstract A spectrodensitometric method has been developed for the quantitative determination of chloramine-T (N-chloro-Ar-sodium-p-toluenesulfonamide) in ice cream. Chloramine-T is extracted and converted into p-toluenesulfonamide (p-TSA) followed by thin layer chromatographic separation of concentrated extracts on silica gel and quantitation of the p-TSA spots from standards and samples by direct scanning with a reflectance densitometer at 228 nm. A linear relationship was obtained between recorded peak area and concentration for 0.5–7.0 μg p-TSA/spot. The reproducibility of the complete method was 2.87% (n = 9 determinations). The detection limit of the scanning procedure was 0.5 μg p-TSA/spot, corresponding to a concentration of 4 mg chloramine-T/kg sample. The average recovery was 88±3% (P = 95%) for 10 ice cream samples spiked with chloramine-T at levels ranging from 10 to 55 mg/kg. The described method was used to assay 146 commercial ice cream and whipped cream samples for chloramine-T.


1999 ◽  
Vol 82 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Gaspar Alemany ◽  
Mohamed Akaârir ◽  
Gamundí Antonio ◽  
Maria C Nicolau

Abstract A review of methodology for separation, detection, and quantitative determination of catecholamines, 5-hydroxytryptamine, and their acidic metabolites in biological tissue and fluids by thin-layer chromatography is presented. Selected procedures, including fluorometric scanning densitometry for catecholamine acetyl derivatives and color scanning densitometry for acids, are described.


1980 ◽  
Vol 63 (3) ◽  
pp. 675-678 ◽  
Author(s):  
Bernadette Mandrou ◽  
Francoise Bressolle

Abstract A procedure is proposed for the quantitative determination of both benzoic and sorbic acids in fruit beverages. This method is carried out by direct ultraviolet spectrophotometric measurements in silica chromatoplates after development with chloroform-methanol-water (65+35+7) and makes use of an internal standard. Applied to samples spiked with benzoic and sorbic acids, this technique gives accurate and reliable results with good recoveries. The sensitivity (about 1 ppm) is particularly convenient for trace level determination.


1987 ◽  
Vol 70 (6) ◽  
pp. 1031-1032
Author(s):  
Yuuko S Endoh ◽  
Ryozo Yamaoka ◽  
Nobuo Sasaki

Abstract A liquid chromatographic (LC) method is described for the quantitative determination of sulfamoyldapsone (2-sulfamoyl-4,4'-diaminodiphenyl sulfone) in swine muscle, liver, kidney, and fat. Sulfamoyldapsone was extracted from tissues with acetonitrile saturated with n-hexane. The extract was washed with n-hexane saturated with acetonitrile, concentrated, and cleaned up by alumina column chromatography. Sulfamoyldapsone was separated on an ODS column by using acetonitrile-methanol-water (6 + 18 + 76) and was detected at 292 nm. Overall average recovery of sulfamoyldapsone added to tissues at levels of 0.1 and 0.5 /μg/g was 93.3% ± 6.0. Detection limit was 0.02 μg/g in these tissues.


1972 ◽  
Vol 9 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Calvert C. Bristol

X-ray powder diffraction methods, successful in quantitative determination of silicate minerals in fine-grained rocks, have been applied to the determination of calcite, dolomite, and magnesite in greenschist facies meta-volcanic rocks. Internal standard graphs employing two standards (NaCl and Mo) have been determined.Carbonate mineral modes (calcite and dolomite) for 6 greenschist facies meta-volcanic rocks obtained by the X-ray powder method have been compared to normative carbonate mineral contents calculated for the same rocks. This comparison showed a maximum variation of 7.7 wt.% between the X-ray modes and the normative carbonate mineral contents of the rocks. Maximum standard deviation for the X-ray modes of these rocks was equivalent to 4.4 wt.%.


Sign in / Sign up

Export Citation Format

Share Document