Determination of Aflatoxins in Beer

Aflatoxins B1, B2, G1, and G2 were determined at parts-per-trillion levels in beer by immunoaffinity column cleanup and reversed-phase liquid chromatography (LC) with fluorescence detection after trifluoroacetic acid derivatization. Silanized vials were necessary for the evaporation step in order to obtain good recoveries of aflatoxins from spiked beer samples. Recoveries averaged 90–104%, 94%, 84–87%, and 89% for aflatoxins B1, B2, G1, and G2, respectively, at levels of 9.7–133 ng B1, 46 ng B2, 35–140 ng G1 and 41 ng G2/L. Detection limits were 19–20 ng/L for aflatoxins B1 and G1 and 15–16 ng/L for aflatoxins B2 and G2 (signal-to-noise ratio = 3:1) obtained by using an excitation wavelength of 360 nm; at 340 nm these detection limits were lowered to about 2 ng/L. Analysis of 24 beer samples, the majority from the United States and Mexico, showed natural contamination of one sample of Mexican beer at 49 ng B1/L when determined at 360 nm excitation, but reanalysis of 23 of the samples using 340 nm excitation indicated that an additional 4 Mexican samples and one Brazilian sample contained anatoxin B1 at low levels (< 10 ng/L).