Determination of Glycarbylamide in Chicken Tissue by High-Performance Liquid Chromatography
Abstract A high-performance liquid chromatographic (HPLC) method for determining glycarbylamide (GB) in chicken tissue was developed. GB was extracted with acetonitrile, followed by solid-phase extraction cleanup using a Bond Elut cartridge column with neutral alumina. After the extract had been evaporated to dryness, the residue was dissolved in 1.0 mL 0.1 N sodium hydroxide. Then 1.0 mL 0.1 M potassium dihydrogen phosphate solution was added to it. HPLC separation was done on a 250 × 4.6 mm id TSK-GEL ODS 80™TM column with 0.05M potassium dihydrogen phosphate as the mobile phase. Ultraviolet detection was done at a wavelength of 260 nm. The calibration curve of standard GB solutions was linear between 0.16 and 3 μg/mL (correlation coefficient, r = 0.999). The recovery of GB from chicken muscle spiked at 0.8 μg/g was 88.6 ± 2.3% (mean ± standard deviation, n = 5), and the lower limit of determination was 0.05 μg/g in chicken muscle.