Extensive evaluation via blinded testing of an UHPLC-MS/MS method for quantitation of ten ergot alkaloids in rye and wheat grains
Abstract Background Ergot alkaloids are mycotoxins produced by the fungus Claviceps, which can contaminate grains and pose a health risk to humans and animals. Validation of an ergot alkaloid method in collaborative projects can be challenging due to instability of analytes, and lack of reliable reference materials and a fully validated reference methods. Objective To extensively evaluate performance of a quantitative UHPLC-MS/MS method to detect ten ergot alkaloids at concentrations between 16–500 ng/g in grains. Method The method performance was evaluated in the Blinded Method Test (BMT) exercise, which allowed organizers to successfully address the challenges. Forty completely blinded test samples were prepared in an independent laboratory and shipped to participating laboratory to analyze on two separate days Results Precision, accuracy and HorRatr values met or exceeded the Food and Drug Administration (FDA) recommendations. The design of the BMT exercise provided a high degree of confidence in data and conclusions drawn. Conclusions The method performed in a manner as expected and the method can be used by the laboratory for routine testing of wheat and rye grains. Highlights Blinded method tests of laboratory methods facilitate validation of tests by evaluating performance in an unbiased manner.