PSIII-25 The effects of varying levels of trace mineral supplementation on mineral balance and antibody concentrations in feedlot cattle

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 287-288
Author(s):  
Brittany A Lippy ◽  
Blake K Wilson ◽  
Colton A Robison
Keyword(s):  
Negative Control ◽  
Feedlot Cattle ◽  
Trace Mineral ◽  
Herpesvirus Type ◽  
Diarrhea Virus ◽  
Mineral Supplementation ◽  
Normal Ranges ◽  
Bovine Herpesvirus Type 1 ◽  
Trends Over Time ◽  
Viral Diarrhea Virus

Abstract Trace mineral (TM) supplementation is critical for optimal performance and health. The objective was to determine the effects of varying levels of TM supplementation on feedlot cattle TM status and antibody concentrations. Angus steers (n = 240; BW = 291 kg ± 27.4) were stratified by arrival BW and source and assigned to 1 of 4 treatments (12 pens/treatment; 5 steers/pen). Treatments included a negative control (CON) in which cattle received no TM supplementation, a requirement treatment (1X) in which cattle received added TM at 2016 Nutrient Requirements of Beef Cattle required levels, a 2 times requirements (2X), and a 4 times requirements (4X) treatment. Selenium was included at 0.1, 0.2, and 0.3 mg/kg for 1X, 2X, and 4X respectively. Treatments were chosen based on reported TM supplementation levels from the 2015 Feedlot Consulting Nutritionist Survey. One steer was chosen at random from each pen to be evaluated for serum and liver TM status and antibody concentrations to respiratory viruses. There was treatment × day interaction for serum Co, and liver Cu and Se (P < 0.0001). Serum Co was greatest for the 4X treatment from d 28 through harvest. Liver Cu was greatest for the 2X and 4X treatments from d 56 through harvest. Liver Se was greatest for 2X and 4X from d 28 through harvest. There was an effect of day on liver Co, Fe, Mn, Mo, and Zn (P < 0.0001) and serum Cu, Mn, Mo, Se, and Zn (P < 0.0019). Concentrations for individual TM had different trends over time, however, all reported values were within normal ranges. Serum Zn was greater at harvest (P = .02). There was an effect of time on Bovine Viral Diarrhea Virus Type 1A, Bovine Herpesvirus Type 1, Bovine Parainfluenza 3 virus antibody titer concentrations (P < 0.0001).

The Effects of Varying Levels of Trace Mineral Supplementation on Feedlot Cattle Performance and Carcass Characteristics

2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 28-28
Author(s):  
Brittany A Lippy ◽  
Blake K Wilson ◽  
Colton A Robison
Keyword(s):  
Block Design ◽  
Carcass Characteristics ◽  
Negative Control ◽  
Feedlot Cattle ◽  
Trace Minerals ◽  
Marbling Score ◽  
Trace Mineral ◽  
Mineral Supplementation ◽  
Experimental Treatments ◽  
Cattle Performance

Abstract The objective of this experiment was to determine the effects of 4 levels of trace mineral supplementation on feedlot cattle. Two hundred and forty Angus crossbred steers (n = 48, BW = 291 ± 27 kg) were stratified by arrival BW and source and randomly assigned to 1 of 4 experimental treatments in a randomized complete block design (12 pens/ treatment; 5 steers/pen). Cattle went through a 35 d depletion period prior to the administration of treatments where cattle received a supplement that included zero trace minerals and was formulated to meet or exceed other nutrient requirements not met by the ration. Cattle were administered treatments after transition to the finishing ration. The treatments included a negative control (CON) which contained no added trace minerals, a requirement treatment (1X) in which cattle received added trace minerals at requirement levels indicated by NASEM (Co 0.15 mg/kg, Cu 10 mg/kg, Fe 50 mg/kg, I 0.5 mg/kg, Mn 20 mg/kg, Se 0.1 mg/kg, Zn 30 mg/kg; NASEM 2016), a 2 times requirement levels (2X) treatment, and a treatment at four times requirement (4X) levels recommended by the NASEM. Iron was kept constant at 50 mg/kg for 1X, 2X, and 4X treatments. Due to feeding laws, Selenium was included at 0.1, 0.2, and 0.3 mg/kg for 1X, 2X, and 4X respectively. Treatment levels were chosen based on the 2015 Feedlot Consulting Nutritionist Survey. There was no difference in BW, ADG, overall DMI and G:F over the course of the experiment (Linear P ≥ 0.12; Quadratic P ≥ 0.17; CON vs Supplements P ≥ 0.47) There was no difference in marbling score, yield grade, back fat, REA, HCW, or dressing percentage between treatments (Linear P ≥ 0.23; Quadratic P ≥ 0.36; CON vs Supplements P ≥ 0.30). In conclusion, the lack of or addition of supplemented trace minerals at or above indicated requirement levels has no effect on cattle performance or carcass characteristics.

scholarly journals Spatial analysis for bovine viral diarrhea virus and bovine herpesvirus type 1 infections in the state of Paraíba, northeastern Brazil

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
Leíse Gomes Fernandes ◽  
Edviges Maristela Pituco ◽  
Adriana Hellmeister de Campos Nogueira Romaldini ◽  
Eliana De Stefano ◽  
Inácio José Clementino ◽  
...  
Keyword(s):  
Spatial Analysis ◽  
Bovine Herpesvirus ◽  
Northeastern Brazil ◽  
Bovine Viral Diarrhea ◽  
Herpesvirus Type ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Bovine Herpesvirus Type 1 ◽  
Viral Diarrhea Virus

scholarly journals Evaluation of the vaccine potential of an equine herpesvirus type 1 vector expressing bovine viral diarrhea virus structural proteins

2007 ◽  
Vol 88 (3) ◽  
pp. 748-757 ◽  
Author(s):  
Cristina T. Rosas ◽  
Patricia König ◽  
Martin Beer ◽  
Edward J. Dubovi ◽  
B. Karsten Tischer ◽  
...  
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Structural Proteins ◽  
Bovine Viral Diarrhea ◽  
Equine Herpesvirus ◽  
Herpesvirus Type ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Equine Herpesvirus Type 1 ◽  
Viral Diarrhea Virus

Bovine viral diarrhea virus (BVDV) is an economically important pathogen of cattle that is maintained in the population by persistently infected animals. Virus infection may result in reproductive failure, respiratory disease and diarrhoea in naïve, susceptible bovines. Here, the construction and characterization of a novel vectored vaccine, which is based on the incorporation of genes encoding BVDV structural proteins (C, Erns, E1, E2) into a bacterial artificial chromosome of the equine herpesvirus type 1 (EHV-1) vaccine strain RacH, are reported. The reconstituted vectored virus, rH_BVDV, expressed BVDV structural proteins efficiently and was indistinguishable from parental vector virus with respect to growth properties in cultured cells. Intramuscular immunization of seronegative cattle with rH_BVDV resulted in induction of BVDV-specific serum neutralizing and ELISA antibodies. Upon experimental challenge infection of immunized calves with the heterologous BVDV strain Ib SE5508, a strong anamnestic boost of the neutralizing-antibody response was observed in all vaccinated animals. Immunized animals presented with reduced viraemia levels and decreased nasal virus shedding, and maintained higher leukocyte counts than mock-vaccinated controls.

140 INTRAUTERINE INOCULATION OF CATTLE WITH BOVINE VIRAL DIARRHEA VIRUS SIMULTANEOUS TO TRANSFER OF IN VIVO-DERIVED BOVINE EMBRYOS

2009 ◽  
Vol 21 (1) ◽  
pp. 169
Author(s):  
J. A. Gard ◽  
M. D. Givens ◽  
P. K. Galik ◽  
M. S. D. Marley ◽  
K. P. Riddell ◽  
...  
Keyword(s):  
Positive Control ◽  
Negative Control ◽  
Bovine Viral Diarrhea ◽  
Bovine Embryos ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
In Vitro Exposure ◽  
Viral Diarrhea Virus

Bovine viral diarrhea virus (BVDV) has been shown to be associated with single transferable in vivo-derived and in vitro-produced bovine embryos despite washing. Hence, the primary objective of this study was to evaluate the potential of BVDV to be transmitted via the intrauterine route at the time of embryo transfer. A total of 10 in vivo-derived Day 7 bovine embryos were nonsurgically collected from a BVDV negative and seronegative donor cow. After collection, embryos were washed in accordance with the International Embryo Transfer Society (IETS) standards. Following washing, embryos were placed into transfer media containing BVDV (SD-1; type 1a). The embryos were immediately aspirated into 0.25-mL straws and transferred into seronegative recipients (Day 0). The total quantity of virus transferred into the uterus of each recipient was 900 to 1000 cell culture infective dose 50 (CCID50)/straw. This amount of virus was previously shown to be consistent with the average amount of BVDV associated with in vivo-derived and in vitro-produced embryos following standard IETS washing procedures after in vitro exposure to virus. The positive control heifer received 1.5 × 106 CCID50/straw of BVDV without an embryo. The negative control heifer received 1.5 × 106 CCID50/straw of heat-inactivated BVDV without an embryo. Serum and buffy coat samples were drawn from all heifers on Days 0, 3, 4, 6, 7, 8, 9, 10, 12, 15, and 30 after inoculation and analyzed for serum neutralizing antibodies and virus, respectively. The positive control heifer and all recipients of virus-exposed embryos exhibited viremia by Day 6 and seroconverted by Day 15. The negative control heifer did not exhibit viremia or seroconvert. All recipients receiving embryos were assessed for pregnancy using transrectal ultrasonography on Day 30 and 6 of 10 heifers were pregnant. On Day 60 the pregnant heifers were again assessed for pregnancy using transrectal ultrasonography. At this time only 1 of the 6 heifers was still pregnant. However, the fetus was determined to be nonviable and was removed via colpotomy. The fetus, fetal fluids and membranes were determined to be positive for BVDV via immunohistochemistry and PCR. Additionally, 213 base pairs of the 5′ nontranslated region of this PCR product were sequenced and found to be consistent with the inoculated strain. Results demonstrate that the average quantity of BVDV associated with bovine embryos after in vitro exposure and washing can result in viremia and seroconversion of seronegative recipients following transfer into the uterus during diestrus.

scholarly journals 42 Effect of injectable trace mineral supplementation on beef cows fertility and overall mineral status

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 45-45
Author(s):  
Stefania Pancini ◽  
Jhon F Currin ◽  
Jamie L Stewart ◽  
Sherrie Clark ◽  
Tracey Redifer ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Post Partum ◽  
Fixed Time ◽  
Beef Cows ◽  
Negative Control ◽  
Trace Mineral ◽  
Mineral Status ◽  
Mineral Supplementation ◽  
Estrus Detection ◽  
Natural Service

Abstract The objective of this study was to evaluate the effect of an injectable multi-mineral complex supplementation on beef cows overall mineral status and fertility. This study involved 9 different locations in the state of Virginia and a total of 1,128 Angus crossbred multiparous cows. All cows had ad libitum access to forage and water, and received mineral supplementation in form of mineral blocks. All cows were enrolled in a 7-d CO-Synch + CIDR fixed-time AI (FTAI) protocol, followed by natural service for an approximately 70-d breeding season. At the initiation of the FTAI protocol (d 0) cows were stratified by days post-partum (82 ± 18 d) and randomly assigned to one of two treatments: 1) One dose of an injectable trace mineral containing zinc (60mg/ml), copper (15mg/ml), selenium (5mg/ml) and manganese (10mg/ml) on d 0 (n = 560; 1 ml/90 kg BW of Multimin® 90, Multimin USA, Ft. Collins, CO); or 2) a negative control with no trace mineral injection (n = 568). BCS was recorded at d 0, 61 and 121. Estrus detection was recorded at d 10 using an estrus detection patch. Pregnancy was diagnosed on days 71 and 131 by ultrasonography. Liver biopsies were collected on days 0 and 45 in a subgroup of 140 cows from both treatments and distributed among all locations. No differences between treatments were detected in days post-partum (P = 0.83), BCS (P ≥ 0.42) and estrus detection (P= 0.12), neither in mineral status of copper, manganese, selenium or zinc (P ≥ 0.28). No effect of treatment was detected (P ≥ 0.20) in AI pregnancy rate (57%) or overall pregnancy rate (93%). In conclusion, one dose of injectable trace mineral administrated 10 days before AI did not improve pregnancy rate or overall mineral status in multiparous beef cows enrolled in FTAI.

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