scholarly journals 182 Uterine gene expression of beef cows in response to supplementation with calcium salts of soybean oil

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 39-39
Author(s):  
Claire L Timlin ◽  
Taylor Parrish ◽  
Nicholas W Dias ◽  
Felipe Santili ◽  
Stefania Pancini ◽  
...  

Abstract We hypothesize that expression of peroxisome proliferator-activated receptors (PPARs; PPARa, PPARg, PPARd) and prostaglandin synthases (PGES, AK1B1) in endometrial tissue of Bos taurus beef cows changes after supplementation with calcium salts of soybean oil (CSSO) rich in omega-6 fatty acids compared to a saturated fat. Multiparous cows, stratified by BW (658.05±16.64 kg) and BCS (7.52±0.18), were randomly assigned to one of two treatments: 1) 0.7 kg/(hd.d-1) corn gluten feed + 0.1 kg/(hd.d-1) of prilled saturated fatty acids (Energy Booster, Milk Specialties, Eden Prairie, MN; CON, n = 12); or 2) 0.7 kg/(hd.d-1) corn gluten feed + 0.1 kg/(hd.d-1) of CSSO (Essentiom, Church and Dwight Co., Inc., Princeton, NJ; CSSO, n = 13). Cows were given a 21-d adaptation period before initiation of the experiment, maintained on common pasture, provided ad libitum access to forage and water, and individually fed treatments. Cows underwent a 7-d CO-Sync+CIDR estrus synchronization protocol beginning d0. Transrectal ultrasonography was performed d14 to determine location of a corpus luteum (CL). Uterine biopsies were collected d18 and d28 of the study, corresponding to d8 and d18 of the estrous cycle, respectively. One biopsy was taken from the anterior-most portion of uterine horns ipsilateral and contralateral to the CL for RNA extraction. Transcript expression was analyzed with reverse transcriptase qPCR and quantified using the 2-ddCt method with GAPDH as reference gene. There was no treatment effect for any transcripts analyzed (P ≥ 0.30), nor an interaction between treatment, horn relative to CL, and day (P ≥ 0.11). Transcript expression increased for both treatments from d18 to d28 for PPARa (0.8±0.18 vs. 1.8±0.22, P = 0.01), PPARd (1.13±0.12 vs. 1.9±0.13, P < 0.01), PPARg (1±0.2 vs. 1.3±0.25, P = 0.01), and AK1B1 (0.62±0.16 vs. 2.34±0.22, P < 0.01). While uterine transcript expression of selected genes increased through the luteal phase, supplementing beef cows with CSSO did not affect transcript expression compared to cows receiving saturated fat.

2017 ◽  
Vol 1 (3) ◽  
pp. 296-303
Author(s):  
E. G. Taylor ◽  
R. P. Lemenager ◽  
K. R. Stewart

Abstract Forty-eight primiparous and diparous Angus-Simmental cows were fed 1 of 3 diets; 1) total mixed ration (TMR) based of corn silage and corn stalks (CON), 2) TMR with 3.3 kg/d DM of corn gluten feed (CGF; MID), or 3) TMR with 6.7 kg/d DM of CGF (HIGH). From 11 ± 5 days post-partum (DPP) to 105 ± 5 DPP, all diets were formulated to be isocaloric for a post-partum ADG targeted at 0.22 kg, but CP exceeded requirements in both CGF diets. Blood samples were collected from cows starting at trial initiation until estrous synchronization for determination of plasma progesterone concentration (7 d intervals), as an indicator of resumption of cyclicity, as well as for plasma urea nitrogen (PUN; 21 d intervals). Milk production was assessed at 62 ± 5 DPP via a weigh-suckle-weigh procedure, and milk samples were collected at 64 ± 5 DPP for composition analysis. A 5 d Co-Synch + Control Internal Drug Release (CIDR) protocol was used and cows were bred by timed artificial insemination (TAI). Trans-rectal ultrasonography was used for the evaluation of the dominant follicle at TAI, as well as pregnancy diagnosis. Nineteen days post-TAI, cow and calf pairs were managed as a single group until weaning (205 ± 5 DPP) and exposed to natural mating for a total of a 60 d breeding season. Dam ADG was not significantly different among treatments (P = 0.849), but, DMI decreased with increasing CGF in the diet (P = 0.049). There were no differences in final BW (P = 0.779), however, final BCS was lower in the HIGH treatment when compared to the MID (P = 0.042). Milk production (P = 0.457), as well as, milk components (P ≥ 0.188) were not different, with the exception of milk fat, which tended to be greater in the HIGH treatment (P = 0.059) when compared to the MID. A treatment by week interaction (P < 0.0001) was found for PUN concentrations. Concentrations were greater in the HIGH treatment compared to the MID treatment at 63, 84, and 105 d, and greater than the CON treatment at all time points except d-42. There were no differences in resumption of cyclicity (P = 0.419), dominant follicle (P = 0.648), or TAI conception rates (P = 0.761). However, season long pregnancy rates were significantly greater in the CGF treatments when compared to the CON (P = 0.009). In summary, feeding high or intermediate amounts of CGF neither has a positive nor negative effect on TAI conception rates of beef cows, however, it positively affected season long pregnancy.


1988 ◽  
Vol 66 (2) ◽  
pp. 522 ◽  
Author(s):  
C. S. Cordes ◽  
K. E. Turner ◽  
J. A. Paterson ◽  
J.G.P. Bowman ◽  
J. R. Forwood

2007 ◽  
Vol 85 (6) ◽  
pp. 1577-1586 ◽  
Author(s):  
R. Driskill ◽  
J. R. Russell ◽  
D. R. Strohbehn ◽  
D. G. Morrical ◽  
S. K. Barnhart ◽  
...  

2021 ◽  
Vol 53 (2) ◽  
Author(s):  
Lucas Fialho de Aragão Bulcão ◽  
Henry Daniel Ruiz Alba ◽  
Gleidson Giordano Pinto de Carvalho ◽  
Maria Leonor Garcia Melo Lopes de Araújo ◽  
Jefferson Rodrigues Gandra ◽  
...  

2011 ◽  
Vol 73 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Menghe H. Li ◽  
Edwin H. Robinson ◽  
Brian G. Bosworth ◽  
Daniel F. Oberle ◽  
Penelope M. Lucas

1988 ◽  
Vol 71 (5) ◽  
pp. 1204-1210 ◽  
Author(s):  
S.L. Gunderson ◽  
A.A. Aguilar ◽  
D.E. Johnson ◽  
J.D. Olson

Author(s):  
J.J. Sindt ◽  
Sean P. Montgomery ◽  
T.B. Farran ◽  
H.J. LaBrune ◽  
R.D. Hunter ◽  
...  

Author(s):  
Leonardo R. Müller ◽  
Daniel I C. G. Gouvêa ◽  
André F. Francischinelli ◽  
Guiherme D. A. Alvarenga ◽  
Pablo C Castagnino ◽  
...  

Abstract This experiment evaluated the effects of feeding whole cottonseed (WC) and/or calcium salts of fatty acids (CSFA) on dry matter intake (DMI), performance, and carcass characteristics of Bos indicus animals receiving a high-concentrate diet during the finishing phase. On day 0, 96 Nellore bulls were blocked according to initial shrunk body weight (BW; 302 ± 26.7 kg) into group pens (4 animals/pen) and, within blocks, pens were randomly assigned to receive: 1) 15% of WC and 2% of CSFA [dry matter (DM) basis] of palm, cottonseed, and soybean oil (15WC; n = 6), 2) 10% of WC and 3% of CSFA (DM basis) of palm, cottonseed, and soybean oil (10WC; n = 6), 3) 5% of WC and 4% of CSFA (DM basis) of palm, cottonseed, and soybean oil (5WC; n = 6), and 4) 0% of WC and 5% of CSFA (DM basis) of palm, cottonseed, and soybean oil (0WC; n = 6). Diets were formulated to be isocaloric, isonitrogenous, and isolipidic. Experimental period lasted 108 days, whereas dry matter intake (DMI) was evaluated daily and blood samples and carcass measurements were obtained on days 0, 55, and 108 of the study. Upon slaughter on day 109, steaks were collected for determination of the chemical and fatty acid (FA) profile of the meat. No treatment effects (P ≥ 0.35) were observed on DMI, performance, average daily gain (ADG), carcass ultrasound measurements, and chemical variables of the steak. Nonetheless, including WC into the diets increased C12:0, C16:0, C16:1 trans-9, C17:0, C18:0, C18:1 cis-9, C18:2 cis-9,cis-12, C18:3 cis-9,cis-12,cis-15, saturated, and unsaturated FA intake (P < 0.01). Moreover, adding WC increased DMI fluctuation and feed efficiency (P = 0.03), but decreased marbling (P ≤ 0.03). A treatment × day interaction was observed (P < 0.01) for serum leptin concentration, as 10WC animals had greater leptin concentration on d 103 vs. other treatments (P < 0.01). Regarding steak FA profile, WC addition into the diet increased C18:2 cis-7,trans-9 and C18:3 cis-9,cis-12,cis-15 (P < 0.001), whereas saturated FA was quadratically affected (P = 0.02) and unsaturated FA was reduced for 15WC (P < 0.04). In summary, increasing levels of CSFA into isolipidic finishing diets containing WC did not negatively impact feedlot performance, but reduced feed efficiency and increased marbling scores of Bos indicus bulls, demonstrating its feasibility as a technology to improve carcass traits of low-marbling animals.


2018 ◽  
Vol 101 (9) ◽  
pp. 7881-7891 ◽  
Author(s):  
J.E. Freitas ◽  
C.S. Takiya ◽  
T.A. Del Valle ◽  
R.V. Barletta ◽  
B.C. Venturelli ◽  
...  

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