Determination of Third-Generation Synthetic Cannabinoids in Oral Fluids

2020 ◽  
Author(s):  
Aitor Sorribes-Soriano ◽  
Josep Verdeguer ◽  
Agustín Pastor ◽  
Sergio Armenta ◽  
Francesc A Esteve-Turrillas
Keyword(s):  
Oral Fluid ◽  
Synthetic Cannabinoids ◽  
High Sensitivity ◽  
Gas Chromatography Mass Spectrometry ◽  
Third Generation ◽  
Model Compounds ◽  
Oral Fluids ◽  
Sensitive Determination ◽  
Packed Sorbent

Abstract A procedure has been developed for the determination of third-generation synthetic cannabinoids in oral fluid samples by using a semi-automated microextraction by packed sorbent (MEPS) procedure and gas chromatography–mass spectrometry (GC–MS) determination. Five synthetic cannabinoids were employed as model compounds 5F-ADB, MMB-CHMICA, THJ-2201, CUMYL-4CN-BINACA and MDMB-CHMCZCA. The most adequate operative conditions for MEPS were evaluated giving quantitative recoveries, from 89 to 124%, in synthetic and field saliva samples spiked with 125 and 250 μg/L of the studied cannabinoids, with the exception of MDMB-CHMCZCA in field saliva samples that provided slightly lower recoveries from 62 to 66%. A high sensitivity was obtained for the proposed MEPS-GC–MS procedure with limits of detection from 10 to 20 μg/L. The obtained results demonstrate the high potential of MEPS-GC–MS combination for semi-automated, selective and sensitive determination of synthetic cannabinoids in oral fluid samples.

scholarly journals Determination of the Synthetic Cannabinoids JWH-122, JWH-210, UR-144 in Oral Fluid of Consumers by GC-MS and Quantification of Parent Compounds and Metabolites by UHPLC-MS/MS

2020 ◽  
Vol 21 (24) ◽  
pp. 9414
Author(s):  
Nunzia La Maida ◽  
Manuela Pellegrini ◽  
Esther Papaseit ◽  
Clara Pérez-Mañá ◽  
Lourdes Poyatos ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Oral Fluid ◽  
High Resolution Mass Spectrometry ◽  
Synthetic Cannabinoids ◽  
Screening Method ◽  
Forensic Toxicology ◽  
Gas Chromatography Mass Spectrometry ◽  
Limit Of Quantification

The consumption of synthetic cannabinoids (SCs) has significantly increased in the last decade and the analysis of SCs and their metabolites in human specimens is gaining interest in clinical and forensic toxicology. A pilot study has been carried out using a combination of an initial last generation gas chromatography-mass spectrometry (GC-MS) screening method for the determination of JWH-122, JWH-210, UR-144) in oral fluid (OF) of consumers and an ultra-high performance liquid chromatography high resolution mass spectrometry (UHPLC-HRMS) confirmatory method for the quantification of the parent compounds and their metabolites in the same biological matrix. OF samples were simply liquid-liquid extracted before injecting in both chromatographic systems. The developed methods have been successfully validated and were linear from limit of quantification (LOQ) to 50 ng/mL OF. Recovery of analytes was always higher than 70% and matrix effect always lower than 15% whereas intra-assay and inter-assay precision and accuracy were always better than 16%. After smoking 1 mg JWH-122 or UR-144 and 3 mg JWH-210, maximum concentration of 4.00–3.14 ng/mL JWH-122, 8.10–7.30 ng/mL JWH-210 ng/mL and 7.40 and 6.81 ng/mL UR-144 were measured by GC-MS and UHPLC-HRMS respectively at 20 min after inhalation. Metabolites of JWH 122 and 210 were quantified in OF by UHPLC-HRMS, while that of UR144 was only detectable in traces. Our results provide for the first time information about disposition of these SCs and their metabolites in consumers OF. Last generation GC-MS has proven useful tool to identify and quantify parent SCs whereas UHPLC-HRMS also confirmed the presence of SCs metabolites in the OF of SCs consumers.

Simultaneous determination of third-generation synthetic cannabinoids in oral fluids using cyclodextrin-silica porous sorbents

2021 ◽  
pp. 106915
Author(s):  
Carolina Belenguer-Sapiña ◽  
Roberto Sáez-Hernández ◽  
Enric Pellicer-Castell ◽  
Sergio Armenta ◽  
Adela.R. Mauri-Aucejo
Keyword(s):  
Simultaneous Determination ◽  
Synthetic Cannabinoids ◽  
Third Generation ◽  
Oral Fluids ◽  
Porous Sorbents

Determination of cannabinoids in oral fluid and urine of “light cannabis” consumers: a pilot study

2018 ◽  
Vol 57 (2) ◽  
pp. 238-243 ◽  
Author(s):  
Roberta Pacifici ◽  
Simona Pichini ◽  
Manuela Pellegrini ◽  
Roberta Tittarelli ◽  
Flaminia Pantano ◽  
...  
Keyword(s):  
Pilot Study ◽  
Oral Fluid ◽  
Urine Samples ◽  
Screening Tests ◽  
Gas Chromatography Mass Spectrometry ◽  
Negative Results ◽  
A Value ◽  
Order Of Magnitude ◽  
Confirmation Analysis

Abstract Background In those countries where cannabis use is still illegal, some manufacturers started producing and selling “light cannabis”: dried flowering tops containing the psychoactive principle Δ-9-tetrahydrocannabinol (THC) at concentrations lower than 0.2% together with variable concentration of cannabidiol (CBD). We here report a pilot study on the determination of cannabinoids in the oral fluid and urine of six individuals after smoking 1 g of “light cannabis”. Methods On site screening for oral fluid samples was performed, as a laboratory immunoassay test for urine samples. A validated gas chromatography-mass spectrometry (GC-MS) method was then applied to quantify THC and CBD, independently from results of screening tests. Results On site screening for oral fluid samples, with a THC cut-off of 25 ng/mL gave negative results for all the individuals at different times after smoking. Similarly, negative results for urine samples screening from all the individuals were obtained. Confirmation analyses showed that oral fluid THC was in the concentration range from 2.5 to 21.5 ng/mL in the first 30 min after smoking and then values slowly decreased. CBD values were usually one order of magnitude higher than those of THC. THC-COOH, the principal urinary THC metabolite, presented the maximum urinary value of 1.8 ng/mL, while urinary CBD had a value of 15.1 ng/mL. Conclusions Consumers of a single 1 g dose of “light cannabis” did not result as positive in urine screening, assessing recent consumption, so that confirmation would not be required. Conversely, they might result as positive to oral fluid testing with some on-site kits, with THC cut-off lower than 25 ng/mL, at least in the first hour after smoking and hence confirmation analysis can be then required. No conclusions can be drawn of eventual chronic users.

scholarly journals Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria

2016 ◽  
Vol 14 (3) ◽  
pp. 366-373 ◽  
Author(s):  
Rodolfo Patussi Correia ◽  
Laiz Cameirão Bento ◽  
Ana Carolina Apelle Bortolucci ◽  
Anderson Marega Alexandre ◽  
Andressa da Costa Vaz ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Retrospective Study ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Laboratory Data ◽  
High Sensitivity ◽  
Laboratory Diagnosis ◽  
Clone Detection ◽  
Technical Advances ◽  
Sensitive Determination

ABSTRACT Objective: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. Methods: A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria. Results: Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection. Conclusion: High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones.

Determination of amphetamine-type stimulants in oral fluid by solid-phase microextraction and gas chromatography–mass spectrometry

2011 ◽  
Vol 696 (1-2) ◽  
pp. 67-76 ◽  
Author(s):  
Daniele Z. Souza ◽  
Paula O. Boehl ◽  
Eloisa Comiran ◽  
Kristiane C. Mariotti ◽  
Flavio Pechansky ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Solid Phase Microextraction ◽  
Oral Fluid ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry
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