Determination of Serum Levels of Interleukin-6, Interleukin-8, Interleukin-10, and Tumor Necrosis-Alpha and their Relationship With The Total Body Surface Area in Children

2019 ◽  
Vol 41 (3) ◽  
pp. 539-543
Author(s):  
Behnam Sobouti ◽  
Yaser Ghavami ◽  
Behnam Asadifar ◽  
Mehrzad Jafarzadeh ◽  
Mohsen Ghelman ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Tumor Necrosis ◽  
Total Body Surface Area ◽  
Serum Levels ◽  
Interleukin 10 ◽  
Interleukin 8 ◽  
Burn Injuries ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tnf Α ◽  
Significant Difference

Abstract There are few studies on the inflammatory processes and the role of cytokines involved in pediatric burn injuries. The present study aims to measure the serum levels of cytokines and their relationship with the degree of burn injury in children. Within the 48 hours of hospitalization, the serum samples were obtained to measure inflammatory cytokines (interleukin-6, interleukin-8, interleukin-10 [IL-6, IL-8, and IL-10] and tumor necrosis factor-alpha [TNF-α]). The level of all of these cytokine factors was assessed by enzyme-linked immunosorbent assay technique. The mean levels of IL-6, IL-8, IL-10, and TNF-α was 18.15 ± 4.77 pg/ml, 59.54 ± 4.59 pg/ml, 8.41 ± 2.09 pg/ml, and 1.48 ± 0.15 pg/ml, respectively, which were higher than the normal range designated for the healthy pediatrics age group. The levels of TNF-α were higher in patients with sepsis (P = .03) and deceased patients (P = .001). There was a statistically significant difference in the levels of IL-8 in patients with second- (.001) and third-degree (.001) burn injuries in comparison to the first-degree burn injuries, and the level of IL-8 was statistically significantly higher in patients with electrical burn injuries in comparison to scald burn injuries (.01). IL-10 was statistically significantly higher in patients with contact burn injuries in comparison to scald (.001) and flame (.03) burn injuries. Cytokine levels in pediatric burn patients increased after severe burn injuries. There was a significant correlation between the levels of IL-8 and the degree of burn injuries.

scholarly journals IL-32 Serum Levels in Coronary Artery Disease Patient and its Relationship with IL-6 and TNF-α Serum Levels

2021 ◽  
Author(s):  
Mina Mohammad-Rezaei ◽  
Reza Ahmadi ◽  
Ali Rafiei ◽  
Arsalan Khaledifar ◽  
Shohila Fatahi ◽  
...  
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Serum Levels ◽  
Arterial Stenosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Subjects ◽  
Tnf Α ◽  
Significant Difference ◽  
Major Vessel ◽  
Artery Disease

Abstract Coronary Artery Disease (CAD) is a chronic inflammatory disease caused by atherosclerosis and arteries become clogged due to plaque formation, fat accumulation, and various sorts of immune cells. IL-32 is a new proinflammatory cytokine, which enhances inflammation through inducing different inflammatory cytokines. The purpose of current research was to assess IL-32 serum levels in coronary artery disease subjects and its relationship with serum levels of IL-6 and TNF-α. Forty-two subjects diagnosed with CAD and thirty-nine control subjects were enrolled in the research. Serum levels of IL-6, TNF-α, and IL-32 were measured using the enzyme-linked immunosorbent assay (ELISA). IL-32, TNF-α, and IL-6 serum levels were significantly higher by 2.7, 3.48, and 3.2-fold in the CAD subjects than in control subjects, respectively. Moreover, no significant difference was found in TNF-α, IL-6 and IL-32 serum levels with the clogged arteries number in the CAD group. TNF-α and IL-32 serum levels in the CAD subjects with cardiac arterial stenosis in one major vessel were significantly increased than CAD subjects with cardiac arterial stenosis in more than one major vessels. ROC curve analysis revealed that serum levels of IL-32, TNF-α, and IL-6 showed good abilities in predicting CAD. Also, Multiple logistic regression analyses suggested that TNF-α, IL-6, and IL-32, serum levels of LDL and ox-LDL were independently related to the presence of CAD, while HDL serum levels were not. TNF-α, IL-32, and IL-6 showed an increase in CAD group and serum levels of these cytokines showed good abilities in predicting CAD. Our data suggested the involvement of TNF-α and IL-32 in the early stage of CAD.

Serum levels of interleukin 6 and tumor necrosis factor-α in hyperthyroid patients before and after propylthiouracil treatment

1995 ◽  
Vol 132 (6) ◽  
pp. 668-672 ◽  
Author(s):  
Ismail Çelik ◽  
Sema Akalin ◽  
Tomris Erbaş
Keyword(s):  
Tumor Necrosis Factor ◽  
Interleukin 6 ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Serum Levels ◽  
Graves Disease ◽  
Tnf Α ◽  
Factor Α ◽  
Hyperthyroid Patients ◽  
Necrosis Factor

Çelik I, Akalin S, Erbaş T. Serum levels of interleukin 6 and tumor necrosis factor-α in hyperthyroid patients before and after propylthiouracil treatment. Eur J Endocrinol 1995;132:668–72. ISSN 0804–4643 Contrary to the usual inhibitory role of tumor necrosis factor-α (TNF-α) thyroid metabolism, it also has specific stimulatory effects in autoimmune thyroid disorders, including induction of HLA class II antigen-presenting cell—T cell interaction. Despite high intrathyroidal concentrations, various studies were not able to demonstrate high serum levels of TNF-α in patients with Graves' disease. To investigate this discrepancy we determined TNF-α and interleukin 6 (IL-6) levels in 25 hyperthyroid patients who responded to propylthiouracil treatment (16 with Graves' disease and nine with toxic multinodular goiter) and compared them with the levels found in euthyroid patients with simple diffuse goiter (n = 15) and normal healthy controls (n = 15). Median IL-6 levels were high in both Graves' disease and toxic multinodular goiter patients before propylthiouracil treatment (23 and 26.5 pg/ml, respectively). After restoring euthyroidism there was a statistically significant decline to near-normal levels (3 and 10 pg/ml, respectively). On the other hand, median serum TNF-α levels were high only in Graves' disease patients (20 pg/ml) and could not be normalized with antithyroid medication (20 pg/ml) compared to that of controls (5 pg/ml). Tumor necrosis factor-α, but not IL-6, was found to be high in the sera of Graves' disease patients when euthyroid, which may be due to an ongoing antigen–antibody interaction, a feature of autoimmune attack. It remains to be determined whether the degree of TNF-α and/or IL-6 elevation will be a predictor of disease recurrence. Ismail Çelik, Section of Oncology, Dept. of Medicine, Hacettepe University Institute of Oncology, Ankara 06100, Turkey

scholarly journals Maternal Expressions (Serum Levels) of Alpha Tumour Necrosis Factor, Interleukin 10, Interleukin 6 and Interleukin 4 in Malaria Infected Pregnant Women Based on Parity in a Tertiary Hospital in Southeast, Nigeria

2020 ◽  
pp. 35-41
Author(s):  
Obeagu, Emmanuel Ifeanyi ◽  
Obeagu, Getrude Uzoma ◽  
Amaeze, Augustine Amaeze ◽  
Asogwa, Eucharia Ijego ◽  
Chukwurah, Ejike Felix ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Tumour Necrosis Factor ◽  
Interleukin 6 ◽  
Tumour Necrosis ◽  
Public Health Problem ◽  
Serum Levels ◽  
Interleukin 10 ◽  
Interleukin 4 ◽  
Tnf Α ◽  
Necrosis Factor

Malaria has been reported as a condition caused by infestation with Plasmodium parasite specie, which is a great public health problem globally, particularly in developing countries like Nigeria. This study was carried out in Federal Medical Centre Umuahia in Abia State, Nigeria. The study was done to determine the maternal serum levels of alpha tumour necrosis factor, interleukin 10, interleukin 6, and interleukin 4 in malaria-infected pregnant women based on parities in Southeast, Nigeria.  A total of 150 subjects between the ages of 18-45 years were recruited for the study comprising 50 subjects each of 3 parities (groups A-C). A commercial ELISA Kit was used to measure all the cytokines. Neither statistically significant differences were found for TNF-α (p=0.636), IL-10 (p=0.892), IL-6 (p=0.306) and IL-4 (p=0.222) between prime parity and second parity nor for TNF-α (p=0.356), IL-10 (p=0.896), IL-6 (p=0.304) and IL-4 (p=0.298) between prime parity and multi-parity of malaria-infected pregnant women. TNF-α (p=0.255), IL-10 (p=0.524), IL-6 (p=0.616), and IL-4 (p=0.672) between second parity and on multi-parity respectively. The study showed no changes in the cytokines studied among the malaria-infected pregnant women based on parities. It shows that the number of pregnancies in women infected with malaria has no changes in the levels of the cytokines studied.

Role of maternal interleukin-8 (IL-8) in normal-term birth in the human

2019 ◽  
Vol 31 (6) ◽  
pp. 1049 ◽  
Author(s):  
Vahid Ehsani ◽  
Maryam Mortazavi ◽  
Khodayar Ghorban ◽  
Maryam Dadmanesh ◽  
Reza Bahramabadi ◽  
...  
Keyword(s):  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Serum Levels ◽  
Interleukin 8 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Normal Term ◽  
Tnf Α ◽  
Prolonged Pregnancy ◽  
Main Factors ◽  
Factor Α

Cytokines are the main factors involved in the normal functions of the placenta and delivery process. The aim of this project was to compare serum levels of interleukin-8 (IL-8), IL-6, tumour necrosis factor α (TNF-α) and transforming growth factor β (TGF-β) in term and prolonged-pregnancy mothers and their neonates. This study was performed on 240 participants including 60 term and prolonged-pregnancy neonates and their corresponding mothers. Serum levels of IL-8, IL-6, TNF-α and TGF-β were evaluated by the enzyme-linked immunosorbent assay technique. The results revealed that IL-8 serum levels were significantly lower in the prolonged-pregnancy mothers and their neonates when compared with term mothers and their neonates. Data analysis also revealed a negative correlation between TGF-β and age of prolonged-pregnancy mothers. A poor positive correlation between IL-6 and head circumference of term neonates was also observed. IL-8 may play crucial roles in the process of on-time delivery and age may significantly affect TGF-β production in prolonged-pregnancy mothers. Pro-inflammatory cytokines, such as IL-6, can also be considered as main factors involved in fetal growth.

scholarly journals Interferon gamma, interleukin 8 and interleukin 10 in serum of patients with the cervical infection and symptoms of the imminent preterm delivery

2013 ◽  
Vol 141 (9-10) ◽  
pp. 623-628 ◽  
Author(s):  
Mirjana Bogavac ◽  
Snezana Brkic ◽  
Dejan Celic ◽  
Natasa Simin ◽  
Jovan Matijasevic ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Preterm Delivery ◽  
Bacterial Infections ◽  
Clinical Symptoms ◽  
Serum Levels ◽  
Interleukin 10 ◽  
Interleukin 8 ◽  
Control Group ◽  
Cervical Infection ◽  
Significant Difference

Introduction. Preterm delivery (PTD), defined as a delivery between 24th and 37th completed week, increases the risk of neonatal morbidity and mortality. There is a growing body of evidence that the intrauterine infection as well as cervicovaginal bacterial infections and Chlamydia infections are possible causes of preterm delivery. Host response to cervicovaginal and/or intrauterine infections is coupled with a release of various inflammatory mediators, many of which are cytokines. Objective. The aim of the study was to find out if cervical infection influenced the serum levels of interferon-? (IFN-?), interleukin 8 (IL-8) and interleukin 10 (IL-10) in patients with the clinical symptoms of the imminent preterm delivery. Methods. A total of 128 pregnant women (from 24 to 30 weeks of gestation) with the clinical symptoms of the imminent preterm delivery were divided in: study group - 85 patients with the cervical infection, and control group - 43 patients without the cervical infection. The level of cytokines in the serum was measured with commercial ELISA tests. Results. No significant difference could be found in serum levels of IFN-? (p=0.632), IL-8 (p=0.712) and IL-10 (p=0.676) between these two investigated groups. Conclusion. The results of our study suggest that there is no significant difference in serum IFN-?, IL-8 and IL-10 concentrations between pregnant women with the symptoms of the imminent preterm delivery who had and had no cervical infection.

scholarly journals Alteration of Serum Levels of Cytokines in Schizophrenic Patients before and after Treatment with Risperidone

2019 ◽  
Author(s):  
Esfandiar Azizi ◽  
Ahmad Zavaran Hosseini ◽  
Sara Soudi ◽  
Ahmad Ali Noorbala
Keyword(s):  
Healthy Subjects ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Syndrome Scale ◽  
Tnf Α ◽  
Significant Difference ◽  
Before And After ◽  
Schizophrenic Patients ◽  
Ifn Γ

A growing body of evidence suggests the existence of abnormalities in the immune system of schizophrenic patients. The current study examined serum levels of interleukin (IL) -1β, IL-6, IL-2,interferon(IFN) -γ, and tumor necrosis factor(TNF)-α in schizophrenic patients before and after treatment with risperidone and correlated levels of these cytokines with symptomatology. The study group consisted of 24 schizophrenic patients as defined by Diagnostic and Statistical Manual of Mental Disorders 4th edition (DSM-IV) criteria and 24 healthy controls. Serum cytokine levels were examined using enzyme-linked immunosorbent assay (ELISA). Schizophrenic symptomatology was assessed with the Positive and Negative Syndrome Scale (PANSS) questionnaire. The serum levels of TNF-α, IL-1β and IL-6 were significantly higher in participants before treatment compared with the healthy controls and after treatment (p<0.001). IFN-γ and IL-2 levels were significantly lower in participants after treatment compared with before treatment and the healthy controls (p<0.001). Except for IL-6 (p<0.05), there was no significant difference in the levels of TNF-α and IL-1β between the patients receiving treatment and the healthy subjects. Moreover, there was no significant difference in levels of IFN-γ and IL-2 between patients before treatment and the healthy subjects. There were no significant correlations between the concentration of cytokines studied and the PANSS. Positive intercorrelations between the production of IFN-γ and IL-2 were detected for sums of all groups(r=0.33, p=0.005). Clinical improvement of treated patients was associated with a reduction in the studied cytokines. It seems that changes in the cytokines level may play a significant role in the psychopathology of these patients.

scholarly journals Changes in serum level of ghrelin, tumor necrosis factor-alpha and interleukin-6 in children with congenital heart disease

2015 ◽  
Vol 6 (5) ◽  
pp. 12-16
Author(s):  
Shiv Kumar Yadav ◽  
Qi Jian YI
Keyword(s):  
Congenital Heart Disease ◽  
Tumor Necrosis Factor ◽  
Interleukin 6 ◽  
Tumor Necrosis ◽  
Congenital Heart ◽  
Tumor Necrosis Factor Α ◽  
Serum Levels ◽  
Tnf Α ◽  
Factor Α ◽  
Necrosis Factor

Objective: To investigate the changes in serum levels of ghrelin, tumor necrosis factor-α and interleukin-6 in Children with Congenital Heart Disease (CHD) at pre and post percutaneous management of the lesions. Material & Methods: We measured the serum levels of ghrelin, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) by enzyme linked immunosorbent assays (ELISA) in 67 patients with CHD and in 20 control subjects; and compared these markers of inflammatory reaction between groups of pre-closure (37 patients) and at the end of 5 minutes (37 patients), 1 month (22 patients) and 3 month (8 patients) post percutaneous management of the lesions. Results: Our results showed that the patients with CHD had significantly higher serum level of Ghrelin, TNF-α and IL-6 in comparison of the controls but gradually return towards the baseline levels at the end of 3 months post percutaneous management of the lesions. Conclusion: The inflammatory reaction is activated in pre-closure and self-limited post-closure in children with CHD. Percutaneous management could improve the inflammatory process, which is safe and effective in children with CHD.DOI: http://dx.doi.org/10.3126/ajms.v6i5.12129 Asian Journal of Medical Sciences Vol.6(5) 2015 12-16   

Sign in / Sign up

Export Citation Format

Share Document