scholarly journals Spatial Distribution of Whitefly Species (Hemiptera: Aleyrodidae) and Identification of Secondary Bacterial Endosymbionts in Tomato Fields in Costa Rica

2020 ◽  
Vol 113 (6) ◽  
pp. 2900-2910
Author(s):  
Xareni Can-Vargas ◽  
Natalia Barboza ◽  
Eric J Fuchs ◽  
Eduardo J Hernández
Keyword(s):  
Spatial Distribution ◽  
Costa Rica ◽  
Bemisia Tabaci ◽  
Restriction Analysis ◽  
Trialeurodes Vaporariorum ◽  
Cytochrome C Oxidase I ◽  
Tomato Production ◽  
Predominant Species ◽  
Pcr Rflp ◽  
Bacterial Endosymbionts

Abstract In Costa Rica, tomato (Solanum lycopersicum Linnaeus) Linnaeus (Solanales: Solanaceae) is one of the crops most severely affected by the whiteflies (Hemiptera: Aleyrodidae) Trialeurodes vaporariorum (Westwood) and the Bemisia tabaci (Gennadius) species complex. The objective of this study was to monitor the spatial distribution and diversity of these species and to detect the presence of secondary bacterial endosymbionts in individuals collected in areas of intensive tomato production. In total, 628 whitefly individuals were identified to the species level using restriction analysis (PCR-RFLP) of a fragment of the mitochondrial cytochrome C oxidase I gene (mtCOI). Trialeurodes vaporariorum was the predominant species, followed by B. tabaci Mediterranean (MED). Bemisia tabaci New World (NW) and B. tabaci Middle East-Asia Minor 1 (MEAM1) were present in lower numbers. The mtCOI fragment was sequenced for 89 individuals and a single haplotype was found for each whitefly species. Using molecular markers, the 628 individuals were analyzed for the presence of four endosymbionts. Arsenophonus Gherna et al. (Enterobacterales: Morganellaceae) was most frequently associated with T. vaporariorum, whereas Wolbachia Hertig (Rickettsiales: Anaplasmataceae) and Rickettsia da Rocha-Lima (Rickettsiales: Rickettsiaceae) were associated with B. tabaci MED. This study confirmed that B. tabaci NW has not been completely displaced by the invasive species B. tabaci MED and B. tabaci MEAM1 present in the country. An association was found between whitefly species present in tomato and certain secondary endosymbionts, elevation was the most likely environmental factor to affect their frequency.

Genetic diversity and geographic distribution of Bemisia tabaci and Trialeurodes vaporariorum in Costa Rica

2019 ◽  
Vol 174 (2) ◽  
pp. 248-261 ◽  
Author(s):  
Natalia M. Barboza ◽  
Paul Esker ◽  
Alice K. Inoue-Nagata ◽  
Enrique Moriones
Keyword(s):  
Genetic Diversity ◽  
Costa Rica ◽  
Bemisia Tabaci ◽  
Geographic Distribution ◽  
Trialeurodes Vaporariorum

Population Dynamics and Distribution of Trialeurodes vaporariorum and Bemisia tabaci (Homoptera: Aleyrodidae) on Poinsettia Following Applications of Three Chemical Insecticides

1993 ◽  
Vol 28 (1) ◽  
pp. 126-135 ◽  
Author(s):  
T. X. Liu ◽  
R. D. Oetting ◽  
G. D. Buntin
Keyword(s):  
Population Dynamics ◽  
Spatial Distribution ◽  
Bemisia Tabaci ◽  
Trialeurodes Vaporariorum ◽  
Immature Stages ◽  
Single Application ◽  
Sweetpotato Whitefly ◽  
Greenhouse Whitefly ◽  
Insecticide Application ◽  
The Third

The effects of three insecticides, bifenthrin, endosulfan and aldicarb, on the within- and between-plant distributions of both greenhouse whitefly (GHWF), Trialeurodes vaporariorum (Westwood), and sweetpotato whitefly (SPWF), Bemisia tabaci (Gennadius), were examined on greenhouse-grown poinsettia using Taylor's Power Law. Insecticide applications affected the spatial distribution of GHWF and SPWF. The populations of immatures of both species surviving an insecticide application on poinsettia were less aggregated within and between plants than untreated populations. Among the three insecticides, the efficacy against the two whiteflies was not significantly different at the end of the seventh week when multiple applications were conducted. Aldicarb caused higher mortality of immature stages than bifenthrin and endosulfan after four weeks following a single application. A single application of bifenthrin and endosulfan affected the distribution of all whitefly stages in the first and second weeks after treatment, whereas aldicarb did not affect the whitefly population until the third week. Insecticidal treatments had little effect on the stratification of whitefly stages within the plant.

Effects of plant species switching on dynamics of amylase and proteinase activity of Bemisia tabaci biotype B and Trialeurodes vaporariorum

2008 ◽  
Vol 16 (4) ◽  
pp. 313 ◽  
Author(s):  
Zhang Guifen ◽  
Lei Fang ◽  
Wan Fanghao ◽  
Ma Jun ◽  
Yang Yuguo
Keyword(s):  
Plant Species ◽  
Bemisia Tabaci ◽  
Trialeurodes Vaporariorum ◽  
Proteinase Activity ◽  
Biotype B ◽  
Bemisia Tabaci Biotype B

scholarly journals Bemisia tabaci Vesicle-Associated Membrane Protein 2 Interacts with Begomoviruses and Plays a Role in Virus Acquisition

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1700
Author(s):  
Yun-Yun Fan ◽  
Yu-Wei Zhong ◽  
Jing Zhao ◽  
Yao Chi ◽  
Sophie Bouvaine ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Bemisia Tabaci ◽  
Species Complex ◽  
Molecular Mechanisms ◽  
Whole Body ◽  
Sri Lankan ◽  
Tomato Production ◽  
Virus Acquisition ◽  
Leaf Curl Virus ◽  
Whitefly Vector

Begomoviruses cause substantial losses to agricultural production, especially in tropical and subtropical regions, and are exclusively transmitted by members of the whitefly Bemisia tabaci species complex. However, the molecular mechanisms underlying the transmission of begomoviruses by their whitefly vector are not clear. In this study, we found that B. tabaci vesicle-associated membrane protein 2 (BtVAMP2) interacts with the coat protein (CP) of tomato yellow leaf curl virus (TYLCV), an emergent begomovirus that seriously impacts tomato production globally. After infection with TYLCV, the transcription of BtVAMP2 was increased. When the BtVAMP2 protein was blocked by feeding with a specific BtVAMP2 antibody, the quantity of TYLCV in B. tabaci whole body was significantly reduced. BtVAMP2 was found to be conserved among the B. tabaci species complex and also interacts with the CP of Sri Lankan cassava mosaic virus (SLCMV). When feeding with BtVAMP2 antibody, the acquisition quantity of SLCMV in whitefly whole body was also decreased significantly. Overall, our results demonstrate that BtVAMP2 interacts with the CP of begomoviruses and promotes their acquisition by whitefly.

scholarly journals PCR-RFLP ITS-based analysis of wine yeast autochthonous strains isolated from different grape cultivars in Taman subregion

2021 ◽  
Vol 285 ◽  
pp. 05020
Author(s):  
Elena Lobodina ◽  
Ivan Suprun ◽  
Natalya Ageeva ◽  
Ekaterina Al-Nakib
Keyword(s):  
Genetic Analysis ◽  
Restriction Analysis ◽  
Its Region ◽  
Wine Yeast ◽  
Product Size ◽  
Rdna Its ◽  
Hanseniaspora Uvarum ◽  
Pcr Rflp ◽  
Autochthonous Yeast ◽  
Rdna Its Region

The studies present the results of morphological, cultural and genetic analysis of the ITS1-ITS4 region of the autochthonous yeast strains genome by using the HaeIII restriction enzyme. On the red and white grapes varieties, based on the morphology of the cells, autochthonous strains belonging to the genus Saccharomyces prevail – 83.3%, what is confirmed by genetic analysis of rDNA ITS region. Restriction analysis showed that all strains of the genus Saccharomyces belong to the species Saccharomyces cerevisiae / S. paradoxus. The percentage of Saccharomyces isolated on the Pervenets Magaracha variety is 86.7%, Krasnostop Anapsky - 80%. The non-Saccharomyces yeast had a product size of 750 bp, presumably of the species Hanseniaspora uvarum.

scholarly journals Estudio comparativo sobre la morfología de Trialeurodes vaporariorum (Westwood) y Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae)

2013 ◽  
Vol 29 (1) ◽  
Author(s):  
Vicente Emilio Carapia Ruiz ◽  
Antonio Castillo-Gutiérrez
Keyword(s):  
Bemisia Tabaci ◽  
Trialeurodes Vaporariorum

Se presenta un estudio morfológico comparativo de las diferentes etapas de desarrollo entre la mosquita blanca del tabaco Bemisia tabaci (Gennadius) y de la mosquita blanca del invernadero Trialeurodes vaporariorum (Westwood); para ilustrar se incluyen dibujos y microfotografías en contraste de fases.

Population Ecology and Spatial Distribution of Whitefly, Bemisia tabaci, (Homoptera: Aleyrodidae) on Brinjal

2021 ◽  
pp. 54-61
Author(s):  
Z. Mohd Rasdi ◽  
I. Fauziah ◽  
K. Fairuz ◽  
M. S. Mohd Saiful
Keyword(s):  
Spatial Distribution ◽  
Bemisia Tabaci ◽  
Population Ecology
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