Ultrastructural Analysis of Mouthparts of Adult Horn Fly (Diptera: Muscidae) From the Brazilian Midwest Region

2020 ◽  
Vol 57 (5) ◽  
pp. 1447-1458 ◽  
Author(s):  
Fernando de Freitas Fernandes ◽  
Ana Cristina Bahia ◽  
Nágila Francinete Costa Secundino ◽  
Paulo Filemon Paolucci Pimenta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphological Characteristics ◽  
Sense Organs ◽  
Horn Fly ◽  
Phylogenetic Studies ◽  
Midwest Region ◽  
Well Differentiated ◽  
Maxillary Palps ◽  
Scanning Electron

Abstract The ultrastructure of the mouthparts of Haematobia irritans (L.) was investigated by scanning electron microscopy. The morphological characteristics of the maxillary palps, labium (prementum and postmentum), labrum, hypopharynx, haustellum, and labellar lobes are described, as well as of the sensilla evidenced on all the surface of the mouthparts, and the set of different positions assumed by the mouth apparatus of this fly. Based on their morphology, 12 well-differentiated sensilla were identified, among three types of cuticular sensilla: trichoidea, coeloconica, and campaniformia. A slight sexual dimorphism in the sensilla patterns found in the mouthparts of H. irritans was evidenced. These observations are discussed with reference to the current literature on the functional morphology of sense organs of Insecta. These results could facilitate the recognition of the chemosensory sensilla by electrophysiological techniques, and foment future taxonomic and phylogenetic studies to better elucidate the evolution of Diptera, Muscomorpha.

scholarly journals Fine Structure of Maxillary Palps in Adults of Hermetia illucens (Diptera: Stratiomyidae)

2020 ◽  
Author(s):  
M Pezzi ◽  
C Scapoli ◽  
M Bharti ◽  
M J Faucheux ◽  
M Chicca ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sexual Dimorphism ◽  
Hermetia Illucens ◽  
The Family ◽  
Sensory Structures ◽  
First Time ◽  
Maxillary Palps ◽  
Scanning Electron

Abstract A relevant species in waste management but also in forensic, medical, and veterinary sciences is the black soldier fly, Hermetia illucens (Linnaeus; Diptera: Stratiomyidae). An ultrastructural study by scanning electron microscopy (SEM) was conducted for the first time on maxillary palps of both sexes, describing in detail the morphology and distribution of sensilla and microtrichia. The maxillary palps, composed of two segments, show sexual dimorphism in length and shape. In both sexes, the first segment is covered only by microtrichia, but the second one is divided into two parts: the proximal one, covered only by microtrichia, and the distal one containing both microtrichia and sensory structures. These structures include two types of sensory pits and one of chaetic sensilla. Due to sexual dimorphism in palp size, females have a higher number of sensory pits. The sexual dimorphism of palps and the presence and role of sensilla in H. illucens was discussed in comparison to other species of the family Stratiomyidae and of other Diptera. This study may represent a base for further investigations on mouthpart structures of this species, involved in key physiological activities, such as feeding, mating and oviposition.

scholarly journals Scanning Electron Microscopy of Circulating Tumor Cells and Tumor-Derived Extracellular Vesicles

Cancers ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 416 ◽  
Author(s):  
Afroditi Nanou ◽  
Mateus Crespo ◽  
Penny Flohr ◽  
Johann De Bono ◽  
Leon Terstappen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Extracellular Vesicles ◽  
Morphological Characteristics ◽  
Castration Resistant Prostate Cancer ◽  
Physical Damage ◽  
Isolation Technique ◽  
Scanning Electron

To explore morphological features of circulating tumor cells (CTCs) and tumor-derived extracellular vesicles (tdEVs), we developed a protocol for scanning electron microscopy (SEM) of CTCs and tdEVs. CTCs and tdEVs were isolated by immunomagnetic enrichment based on their Epithelial Cell Adhesion Molecule (EpCAM) expression or by physical separation through 5 μm microsieves from 7.5 mL of blood from Castration-Resistant Prostate Cancer (CRPC) patients. Protocols were optimized using blood samples of healthy donors spiked with PC3 and LNCaP cell lines. CTCs and tdEVs were identified among the enriched cells by fluorescence microscopy. The positions of DNA+, CK+, CD45− CTCs and DNA−, CK+, CD45− tdEVs on the CellSearch cartridges and microsieves were recorded. After gradual dehydration and chemical drying, the regions of interest were imaged by SEM. CellSearch CTCs retained their morphology revealing various shapes, some of which were clearly associated with CTCs undergoing apoptosis. The ferrofluid was clearly distinguishable, shielding major portions of all isolated objects. CTCs and leukocytes on microsieves were clearly visible, but revealed physical damage attributed to the physical forces that cells exhibit while entering one or multiple pores. tdEVs could not be identified on the microsieves as they passed through the pores. Insights on the underlying mechanism of each isolation technique could be obtained. Complete detailed morphological characteristics of CTCs are, however, masked by both techniques.

scholarly journals Micromorphological characterization of adhesive interface of sound dentin and total-etch and self-etch adhesives

2015 ◽  
Vol 68 (1-2) ◽  
pp. 10-16
Author(s):  
Milan Drobac ◽  
Igor Stojanac ◽  
Bojana Ramic ◽  
Milica Premovic ◽  
Ljubomir Petrovic
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphological Characteristics ◽  
Adhesive Bond ◽  
Hybrid Layer ◽  
Nih Image ◽  
Dental Tissues ◽  
Composite Systems ◽  
Self Etch ◽  
Scanning Electron

Introduction. The ultimate goal in restorative dentistry has always been to achieve strong and permanent bond between the dental tissues and filling materials. It is not easy to achieve this task because the bonding process is different for enamel and dentin - dentin is more humid and more organic than enamel. It is moisture and organic nature of dentin that make this hard tissue very complex to achieve adhesive bond. One of the first and most widely used tools for examining the adhesive bond between hard dental tissues and composite restorative materials is scanning electron microscopy. The aim of this study was scanning electron microscopy analyzes the interfacial micro morphology of total-etch and self-etch adhesives. Material and Methods. Micro morphological characteristics of interface between totaletch adhesive (Prime & Bond NT) in combination with the corresponding composite (Ceram X Mono) were compared with those of self-etching adhesive (AdheSE One) in combination with the corresponding composite (Tetric EvoCeram). The specimens were observed under 1000 x magnification of scanning electron microscopy (JEOL, JSM-6460 Low Vacuum). Measurement of the thickness of the hybrid layer of the examined composite systems was performed with the software of the device used (NIH Image ?nalyser). Results. Micromorphological analysis of interface showed that the hybrid layer in sound dentin was well formed, its average thickness being 2.68 ?m, with a large number of resin tags and a large amount of lateral branches for specimens with a composite system Prime & Bond NT - Ceram X Mono. However, the specimens with composite systems Adhese One - Tetric EvoCeram did not show the presence of hybrid layer and the resin tags were poorly represented. Conclusion. The results of this study suggest that total-etch adhesives bond better with sound dentin than self-etch adhesives.

Copulatory system of the spider crab Libinia spinosa (Crustacea: Brachyura: Majoidea)

2011 ◽  
Vol 91 (8) ◽  
pp. 1617-1625 ◽  
Author(s):  
M.P. Sal Moyano ◽  
M.A. Gavio ◽  
E.I. Cuartas
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Functional Morphology ◽  
Carapace Width ◽  
Morphological Characteristics ◽  
Spider Crab ◽  
Flexible Membrane ◽  
Physiological Maturity ◽  
Different Types ◽  
Scanning Electron

Functional morphology of the male gonopods, female holding systems and vulvae of Libinia spinosa are described using scanning electron microscopy and histological sections, establishing differences between immature and mature individuals. The first gonopod of mature males, larger than 35.1 mm of carapace width (CW), is long, slender, and ending in two wing-like processes; abdominal, characterized by the presence of a sub-terminal seminal canal, and sternal, closed and where the cuticle holds setae. In immature males, smaller than 29.9 mm CW, both processes are shorter, and the tip of the abdominal process closed. Six different types of setae are described for both gonopods. Internally, the gonopods have rosette glands in immature and mature individuals. The presence of secretions, which may be involved in the moulting process, was observed in immature males towards the external cuticle corresponding to the rosette glands. The rosette glands may be related to reproduction in mature males. Females presented a press-button holding system and a biconcave vulva. In immature females, smaller than 36.1 mm CW, the vulva aperture was closed by integument bulging to the outside. In mature females, larger than 42.6 mm CW, the locking system was almost fused to the cuticle, and the vulvae showed an open biconcave form with a more inner flexible membrane. Morphological characteristics shown by the gonopods of mature males develop synchronously with the acquisition of physiological maturity, not with morphometrical maturity. The mature holding systems and vulvae of females are developed synchronously with physiological and morphometrical maturity.

scholarly journals TO THE QUESTION OF AN INTEGRATED RESEARCH OF BIOLOGICAL DAMAGE TO FIBROUS MATERIALS BY THE METHOD OF RASTER ELECTRON MICROSCOPY

2019 ◽  
Vol 20 (2) ◽  
pp. 522-534
Author(s):  
T. Balinyan ◽  
L. Derecha ◽  
Yu. Nosatenko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Higher Plants ◽  
Morphological Characteristics ◽  
Fibrous Materials ◽  
Blue Green Algae ◽  
Biological Damage ◽  
Wide Range ◽  
Scanning Electron ◽  
Comprehensive Study

The article considers the need for a comprehensive study of biological damage to fibrous materials by scanning electron microscopy. The main types and characteristics of fibers and fibrous materials, their types of damage, in particular, biological, and the mechanism of their formation are described. It is shown that with modern methods for studying morphological characteristics, the most effective is the method of scanning electron microscopy, which makes it possible to directly study the object in a wide range of magnifications. The use of scanning electron microscopy makes it possible to identify qualitatively new volumetric microsigns when conducting studies of fibrous materials. Biological damage agents (biofactors) are considered — microbiological (bacteria, microbes, fungi, blue-green algae), phytological (mosses, lichens, higher plants, algae), zoological (insects, birds, mammals). Attention is focused on the study of injuries caused by mold caused by moths, dogs, etc. Conducting a comprehensive study of various types of damage to materials of various fibrous nature allows us to obtain an information database, the possibility of differentiating chemical, mechanical, thermal and biological damage, identifying microsigns that individualize one or another object (factor) of action, influence, increasing the potential for obtaining trace information about the actual data and circumstances of the event in those cases when only by external morphological features of the diagnosis It is not possible to repair damage. The data obtained indicate the effectiveness of the chosen research area. The results of the studies are positive for creating the optimal research scheme, methods of microscopic studies of damage to materials of fibrous nature in order to solve diagnostic, identification and situational tasks of forensic examination.

scholarly journals Taxonomic significance of pollen morphology in Succisa and Succisella

2019 ◽  
Vol 55 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Zoya M. Tsymbalyuk ◽  
Sergei L. Mosyakin ◽  
Lyudmila M. Nitsenko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Taxonomic Significance ◽  
Oblate Spheroidal ◽  
Phylogenetic Studies ◽  
Molecular Phylogenetic ◽  
Scanning Electron

Abstract Pollen morphology of representatives of the genera Succisa and Succisella in the flora of Ukraine was studied using light and scanning electron microscopy. Pollen grains in both taxa were tricolpate, prolate, rarely oblate-spheroidal or spheroidal; large-sized. Their outline in polar view was subcircular, rarely trilobate, in equatorial view elliptical or rarely circular. Colpi short, of variable width, margins irregular with distinct, narrow margo, and blunt or acute ends. Exine sculpture was echinate-microechinate. The revealed characteristics of pollen grains are taxonomically significant at the generic and specific levels, and they can be used in pollen analysis. Palynomorphological data are consistent with the results of recent molecular phylogenetic studies. Data on pollen morphology also confirm taxonomic circumscription of Succiseae V. Mayer & Ehrend. Pollen grains in Succiseae are characterized by a tricolpate aperture type, which was probably ancestral in Dipsacaceae s. str.

scholarly journals Description of the Demicyclic life Cycle of Puccinia Sherardiana in Sphaeralcea Angustifolia in Mexico.

2021 ◽  
Author(s):  
Magnolia Moreno-Velázquez ◽  
Jesús Ricardo Sánchez-Pale ◽  
Ricardo Tapia-Nuño ◽  
Moisés Camacho-Tapia ◽  
José Manuel Cambrón-Crisantos ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Life Cycle ◽  
Pathogenic Fungus ◽  
Morphological Characteristics ◽  
Identity Verification ◽  
Pathogenicity Tests ◽  
Koch Postulates ◽  
Scanning Electron ◽  
Observation Period

Abstract During 2017-2019, leaves and stems with dark brown lesions containing hypophyllous telia surrounded by chlorotic halos were collected from Sphaeralcea angustifolia plants located in Axapusco, State of Mexico. Based on the morphological characteristics of pycnia, aecia and telia observed by light microscopy and scanning electron microscopy, the fungus Puccinia sherardiana was identified. Uredial stage was not present during the observation period. Identity verification was carried out by phylogenetic analysis with sequences of part of the 28S gene from ribosomal DNA. In addition, pathogenicity tests were done on S. angustifolia leaves by inoculating teliospores. The inoculated plants developed symptoms 15 days after inoculation, the signs beginning with the presence of aecia in the epidermis of the host and later telia were formed, completing the Koch Postulates. Puccinia sherardiana was previously described as a rust with a microcyclic life cycle on species of the genera Alcea, Malvastrum, Sidalcea and Sphaeralcea , belonging to the Malvaceae family, however, this study revealed that this plant pathogenic fungus has a demicyclic life cycle.

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