Gracilis Flap for Perineal Reconstruction and Functional Restoration for the Treatment of Fecal Incontinence

Loss Of Function ◽

Sphincter Reconstruction ◽

Tissue Reconstruction ◽

Perineal Wounds

Preoperative evaluation and management of complex perineal wounds must account for any history of previous surgery, radiation, or trauma; the size and surface area following resection or debridement; and the loss of function or structural integrity. The gracilis muscle or myocutaneous flap is a local, well-vascularized option suitable for perineal soft tissue reconstruction, particularly for moderately sized defects of the groin. The use of functional gracilis muscle for anal sphincter reconstruction may also be considered for the treatment of fecal incontinence due to significant neurologic, traumatic, or iatrogenic injury, as well as for congenital abnormalities.

Osteolysis of Structural Autograft after Calcaneocuboid Distraction Arthrodesis for Stage II Posterior Tibial Tendon Dysfunction

Foot & Ankle International ◽

10.1177/107110070202300609 ◽

2002 ◽

Vol 23 (6) ◽

pp. 521-529 ◽

Cited By ~ 41

Author(s):

Stephen F. Conti ◽

Yue Shuen Wong

Keyword(s):

Structural Integrity ◽

Nonoperative Treatment ◽

Stage Ii ◽

Posterior Tibial Tendon ◽

Posterior Tibial Tendon Dysfunction ◽

Lateral Column ◽

Posterior Tibial ◽

Tendon Lengthening ◽

Surgical treatment of stage II posterior tibial tendon dysfunction that fails nonoperative treatment is amenable to operative treatment. This commonly consists of a medial soft-tissue reconstruction and lateral column lengthening. We report on 32 patients undergoing distraction calcaneocuboid arthrodesis using an autogenous tricortical iliac crest graft. Almost 50% of patients did not go on to complete and uneventful incorporation of the graft at the arthrodesis site. Two different types of failure were noted. The first was classic nonunion which maintained structural integrity of the graft. The second was osteolysis and collapse of the graft accompanying the nonunion. Risk factors included smoking, K-wire fixation and possibly larger deformities requiring Achilles tendon lengthening. Complications of nonunion were salvageable with reoperation.

Soft Tissue Reconstruction After Epulis Excision

10.26226/morressier.5ac383272afeeb00097a45e2 ◽

2018 ◽

Author(s):

Kubra Kundak

Keyword(s):

Soft Tissue ◽

Autologous Fat Transfer and Cell-Based Strategies for Soft Tissue Reconstruction

Current Tissue Engineering ◽

10.2174/2211542002999140109150722 ◽

2014 ◽

Vol 3 (1) ◽

pp. 62-69

Author(s):

Kshemendra Senarath-Yapa ◽

Rebecca Garza ◽

Adrian McArdle ◽

Graham Walmsley ◽

Michael Hu ◽

...

Keyword(s):

Soft Tissue ◽

Fat Transfer ◽

Autologous Fat ◽

Autologous Fat Transfer ◽

Motility Improvement of Biomimetic Trachea Scaffold via Hybrid 3D-Bioprinting Technology

Polymers ◽

10.3390/polym13060971 ◽

2021 ◽

Vol 13 (6) ◽

pp. 971

Author(s):

Young Soo Yu ◽

Chi Bum Ahn ◽

Kuk Hui Son ◽

Jin Woo Lee

Keyword(s):

Rotation Angle ◽

Thick Layer ◽

Cartilage Tissue ◽

3D Bioprinting ◽

Rotating Beam ◽

Rotational Angle ◽

Gelatin Hydrogel ◽

Tissue Reconstruction ◽

Neck Rotation

A trachea has a structure capable of responding to various movements such as rotation of the neck and relaxation/contraction of the conduit due to the mucous membrane and cartilage tissue. However, current reported tubular implanting structures are difficult to impelement as replacements for original trachea movements. Therefore, in this study, we developed a new trachea implant with similar anatomical structure and mechanical properties to native tissue using 3D printing technology and evaluated its performance. A 250 µm-thick layer composed of polycaprolactone (PCL) nanofibers was fabricated on a rotating beam using electrospinning technology, and a scaffold with C-shaped cartilage grooves that mimics the human airway structure was printed to enable reconstruction of cartilage outside the airway. A cartilage type scaffold had a highest rotational angle (254°) among them and it showed up to 2.8 times compared to human average neck rotation angle. The cartilage type showed a maximum elongation of 8 times higher than that of the bellows type and it showed the elongation of 3 times higher than that of cylinder type. In cartilage type scaffold, gelatin hydrogel printed on the outside of the scaffold was remain 22.2% under the condition where no hydrogel was left in other type scaffolds. In addition, after 2 days of breathing test, the amount of gelatin remaining inside the scaffold was more than twice that of other scaffolds. This novel trachea scaffold with hydrogel inside and outside of the structure was well-preserved under external flow and is expected to be advantageous for soft tissue reconstruction of the trachea.

A modified surgical approach for hard and soft tissue reconstruction of severe periimplantitis defects: laser-assisted periimplant defect regeneration (LAPIDER)

International Journal of Implant Dentistry ◽

10.1186/s40729-020-00218-6 ◽

2020 ◽

Vol 6 (1) ◽

Author(s):

Robert Noelken ◽

Bilal Al-Nawas

Keyword(s):

Soft Tissue ◽

Surgical Approach ◽

Codon harmonization reduces amino acid misincorporation in bacterially expressed P. falciparum proteins and improves their immunogenicity

AMB Express ◽

10.1186/s13568-019-0890-6 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Neeraja Punde ◽

Jennifer Kooken ◽

Dagmar Leary ◽

Patricia M. Legler ◽

Evelina Angov

Keyword(s):

Protein Structure ◽

Amino Acid ◽

Codon Usage ◽

Dna Sequences ◽

Structural Integrity ◽

Host Cells ◽

Loss Of Function ◽

Species Specific ◽

And Function ◽

The Impact

Abstract Codon usage frequency influences protein structure and function. The frequency with which codons are used potentially impacts primary, secondary and tertiary protein structure. Poor expression, loss of function, insolubility, or truncation can result from species-specific differences in codon usage. “Codon harmonization” more closely aligns native codon usage frequencies with those of the expression host particularly within putative inter-domain segments where slower rates of translation may play a role in protein folding. Heterologous expression of Plasmodium falciparum genes in Escherichia coli has been a challenge due to their AT-rich codon bias and the highly repetitive DNA sequences. Here, codon harmonization was applied to the malarial antigen, CelTOS (Cell-traversal protein for ookinetes and sporozoites). CelTOS is a highly conserved P. falciparum protein involved in cellular traversal through mosquito and vertebrate host cells. It reversibly refolds after thermal denaturation making it a desirable malarial vaccine candidate. Protein expressed in E. coli from a codon harmonized sequence of P. falciparum CelTOS (CH-PfCelTOS) was compared with protein expressed from the native codon sequence (N-PfCelTOS) to assess the impact of codon usage on protein expression levels, solubility, yield, stability, structural integrity, recognition with CelTOS-specific mAbs and immunogenicity in mice. While the translated proteins were expected to be identical, the translated products produced from the codon-harmonized sequence differed in helical content and showed a smaller distribution of polypeptides in mass spectra indicating lower heterogeneity of the codon harmonized version and fewer amino acid misincorporations. Substitutions of hydrophobic-to-hydrophobic amino acid were observed more commonly than any other. CH-PfCelTOS induced significantly higher antibody levels compared with N-PfCelTOS; however, no significant differences in either IFN-γ or IL-4 cellular responses were detected between the two antigens.

Radical treatment of severe open fractures of extremities by orthoplastic surgery: a 10-year retrospective study

Journal of Orthopaedic Surgery and Research ◽

10.1186/s13018-021-02479-2 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Zhao Yang ◽

Chao Xu ◽

Yong-Gang Zhu ◽

Jun Li ◽

Zi-Xiang Wu ◽

...

Keyword(s):

Soft Tissue ◽

Infection Rate ◽

Clinical Data ◽

Open Fractures ◽

Satisfactory Outcome ◽

Tissue Damages ◽

Tissue Reconstruction ◽

Severe Soft Tissue

Abstract Objective This study aimed to retrospectively analyze clinical data of a series of patients with severe open fractures of extremities (Gustilo IIIb or IIIc), who achieved a satisfactory outcome through radical orthoplastic surgery, so as to provide a reference for determining the treatment of severe open fractures of extremities. Methods The clinical data of 41 consecutive patients with severe open fracture (Gustilo IIIb or IIIc) of the limb, who underwent successful surgical debridement, fixation, and soft tissue reconstruction in one stage between January 2008 and January 2019, were retrospectively reviewed. Postoperative indicators, including infection rate and union time, were acquired by a regular follow-up and analyzed. Results The mean (±SD) age of the patients was 38 ± 16 years. A total of 90 open fractures and severe soft tissue damages were analyzed. The soft tissue cover was achieved within 72 h. The overall rate of infection was 14.6% (6/41). Sex and the Mangled Extremity Severity Score were associated with infection. The median union time of 40 patients (one amputation) was 32 weeks. Conclusion The overall rate of infection exhibited a lower tendency in this study compared with previous studies on high-grade open fractures following a two-stage orthopedic approach. The consequence of infection rate and union time was similar to that in previous studies. These results indicated that the single-stage radical orthoplastic treatment was an effective and reliable option for reconstructing severe open fractures.