scholarly journals Genes for prostaglandin synthesis, transport and inactivation are differentially expressed in human uterine tissues, and the prostaglandin F synthase AKR1B1 is induced in myometrial cells by inflammatory cytokines

2010 ◽  
Vol 17 (1) ◽  
pp. 1-13 ◽  
Author(s):  
R. J. Phillips ◽  
H. Al-Zamil ◽  
L. P. Hunt ◽  
M. A. Fortier ◽  
A. Lopez Bernal
Keyword(s):  
Inflammatory Cytokines ◽  
Prostaglandin Synthesis ◽  
Differentially Expressed ◽  
Myometrial Cells ◽  
Prostaglandin F

scholarly journals IRF5 is increased in labouring myometrium and regulates pro-labour mediators

Reproduction ◽  
2018 ◽  
Vol 156 (3) ◽  
pp. 207-218
Author(s):  
Ratana Lim ◽  
Gillian Barker ◽  
Martha Lappas
Keyword(s):  
Preterm Birth ◽  
Inflammatory Response ◽  
Inflammatory Cytokines ◽  
Treatment Options ◽  
Adult Life ◽  
Human Myometrium ◽  
Myometrial Cells ◽  
Associated Proteins ◽  
Term Labour ◽  
Pro Inflammatory Cytokines

Preterm birth continues to be the leading cause of neonatal mortality and morbidities that can extend into adult life. Few treatment options stem from our incomplete understanding of the mechanisms of human labour and delivery. Activation of the inflammatory response in gestational tissues by inflammation and/or infection leads to the production of pro-inflammatory and pro-labour mediators, thus preterm birth. Interferon regulatory factor 5 (IRF5) has recently emerged as an important pro-inflammatory transcription factor involved in acute and chronic inflammation. The aims of this study were to determine the expression of IRF5 in human myometrium from labouring and non-labouring women, and whether IRF5 is involved in the genesis of pro-inflammatory and pro-labour mediators induced by pro-inflammatory cytokines or toll-like receptor (TLR) ligands. IRF5 mRNA and protein expression was significantly higher in human myometrium after spontaneous term labour, compared to non-labouring tissues. IRF5 mRNA expression was also significantly higher in primary myometrial cells treated with the pro-inflammatory cytokines IL1B or TNF. In primary myometrial cells, IRF5 knockdown by siRNA (siIRF5) was associated with significantly decreased expression and or secretion of pro-inflammatory cytokines (IL1A, IL6), chemokines (CXCL8, CCL2), adhesion molecules (ICAM1, VCAM1) and contraction-associated proteins PTGS2, PGF2α and PTGFR when in the presence of IL1B, TNF, fsl-1 (TLR2/6 ligand) or flagellin (TLR5 ligand). siIRF5-transfected cells also displayed decreased NF-κB RELA transcriptional activity in the presence of these preterm birth mediators. Our study suggests a novel role for IRF5 in the regulation of the inflammatory response in human myometrium.

DIFFERENTIALLY EXPRESSED GENES IN L6 RAT SKELETAL MUSCLE MYOBLASTS AFTER INCUBATION WITH INFLAMMATORY CYTOKINES

Cytokine ◽  
2001 ◽  
Vol 13 (6) ◽  
pp. 342-348 ◽  
Author(s):  
Volker Adams ◽  
Karsten Lenk ◽  
Andreas Schubert ◽  
Stephan Gielen ◽  
Gerhard Schuler ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Differentially Expressed Genes ◽  
Inflammatory Cytokines ◽  
Differentially Expressed ◽  
Rat Skeletal Muscle

Signal transduction in rat myometrial cells: comparison of the actions of endothelin-1, oxytocin and prostaglandin F2α

1995 ◽  
Vol 133 (4) ◽  
pp. 467-474 ◽  
Author(s):  
Miklós Molnár ◽  
Frank Hertelendy
Keyword(s):  
Signal Transduction ◽  
Phospholipase C ◽  
Inositol Phosphate ◽  
Rank Order ◽  
Prostaglandin F2α ◽  
Intact Cells ◽  
Pregnant Rat ◽  
Endothelin 1 ◽  
Myometrial Cells ◽  
Prostaglandin F

Molnár M, Hertelendy F. Signal transduction in rat myometrial cells: comparison of the actions of endothelin-1, oxytocin and prostaglandin F2α. Eur J Endocrinol 1995;133:467–74. ISSN 0804–4643 The objectives of this study were to evaluate and compare the actions of endothelin-1 (ET-1), oxytocin, prostaglandin F2α (PGF2α) and inositol 1,4,5-trisphosphate (IP3) on Ca2+ mobilization in permeabilized rat myometrial cells and to examine the activation of the inositol lipid cycle in intact myocytes. Cells were isolated from late pregnant rat myometrium and used as confluent monolayers after a single passage. All four agonists caused a biphasic release of45Ca2+ from non-mitochondrial pool(s), with the rank order of potency: oxytocin > PGF2α > ET-1 > IP3. Inhibitors of phospholipase C blocked ET-1-and oxytocin-promoted but not PGF2α-promoted 45Ca2+ efflux. Similarly, heparin, an IP3 receptor blocker, failed to inhibit PGF2α-induced Ca2+ release while inhibiting the action of the other agonists. Endothelin-1 and oxytocin stimulated inositol phosphate accumulation at concentrations similar to those that promoted 45Ca2+ efflux, whereas about 100 times higher concentrations of PGF2α were needed to activate this signaling pathway in intact cells. It is concluded that the primary action of PGF2α in myometrial cells is to enhance Ca2+ influx, whereas oxytocin and ET-1 receptors are coupled to phospholipase C, generating IP3 and raising the intracellular concentration of free Ca2+ from intracellular as well as extracellular sources. Frank Hertelendy, Dept. Ob/Gyn, St Loufis University Health Sciences Center, 3635 Vista Ave at Grand Blvd, St Louis, MO 63110-0250, USA

Indomethacin inhibits the effects of oestrogen in the anterior pituitary gland of the rat

1989 ◽  
Vol 121 (3) ◽  
pp. 513-519 ◽  
Author(s):  
D. G. Rosental ◽  
G. A. Machiavelli ◽  
A. C. Cherñavsky ◽  
N. Sterin Speziale ◽  
J. A. Burdman
Keyword(s):  
Pituitary Gland ◽  
Binding Sites ◽  
Anterior Pituitary ◽  
Subcellular Fraction ◽  
Culture Media ◽  
Biological Effects ◽  
Prostaglandin Synthesis ◽  
Anterior Pituitary Gland ◽  
Prostaglandin F ◽  
Prolactin Synthesis

ABSTRACT Two inhibitors of prostaglandin synthesis, indomethacin and aspirin, blocked the increase of oestrogen-binding sites in the nuclear subcellular fraction, an increase which occurs after the administration of oestradiol. Consequently the biological effects of oestrogens in the anterior pituitary gland of the rat (prolactin synthesis, concentration of progesteronebinding sites and cell proliferation) are diminished. The anterior pituitary gland synthesized prostaglandin F2α (PGF2α), PGE2 and PGD2 from arachidonic acid. This synthesis was blocked when indomethacin was added to the culture media. Oestrogen increased the concentration of PGE2: an increase that was partially prevented by indomethacin. Prostaglandins may have an important role on the effects of oestrogen in the anterior pituitary gland of the rat. Journal of Endocrinology (1989) 121, 513–519

scholarly journals MicroRNAome: Potential and Veritable Immunomolecular Therapeutic and Diagnostic Baseline for Lingering Bovine Endometritis

2020 ◽  
Vol 7 ◽  
Author(s):  
Ayodele Olaolu Oladejo ◽  
Yajuan Li ◽  
Xiaohu Wu ◽  
Bereket Habte Imam ◽  
Wenxiang Shen ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Inflammatory Cytokines ◽  
Messenger Rna ◽  
Inflammatory Diseases ◽  
Pharmaceutical Preparations ◽  
Differentially Expressed ◽  
Signal Pathways ◽  
Pathogen Invasion ◽  
Control And Prevention ◽  
Cellular Signal

The bovine endometrium is a natural pathogen invasion barrier of the uterine tissues' endometrial epithelial cells that can resist foreign pathogen invasion by controlling the inflammatory immune response. Some pathogens suppress the innate immune system of the endometrium, leading to prolonged systemic inflammatory response through the blood circulation or cellular degradation resulting in bovine endometritis by bacterial endotoxins. The microRNA (miRNA) typically involves gene expression in multicellular organisms in post-transcription regulation by affecting both the stability and the translation of messenger RNA. Accumulated evidence suggests that miRNAs are important regulators of genes in several cellular processes. They are a class of endogenous non-coding RNAs, which play pivotal roles in the inflammatory response of reproductive diseases. Studies confirmed that miRNAs play a key regulatory role in various inflammatory diseases by mediating the molecular mechanism of inflammatory cytokines via signal pathways. It implicates some miRNAs in the occurrence of bovine endometritis, resorting to regulating the activities of some inflammatory cytokines, chemokine, differentially expressed genes, and protein through modulating of specific cellular signal pathways functions. This review dwells on improving the knowledge of the role of miRNAs involvement in inflammatory response as to early diagnosis, control, and prevention of bovine endometritis and consequently enlighten on the molecular improvement of the genes coded by various differentially expressed miRNA through the need to adopt recent genetic technologies and the development of new pharmaceutical preparations.

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