DNA repair modifies the site and strand specificity of ethyl methanesulfonate mutagenesis in yeast

Mutagenesis ◽  
1992 ◽  
Vol 7 (6) ◽  
pp. 461-469 ◽  
Author(s):  
Bernard A. Kunz ◽  
Maila Gabriel ◽  
Xiaolin Kang ◽  
Susanne E. Kohalmi ◽  
Karin A. Terrick
1981 ◽  
Vol 153 (3) ◽  
pp. 595-604 ◽  
Author(s):  
D P Dialynas ◽  
M R Loken ◽  
A L Glasebrook ◽  
F W Fitch

To investigate the role of Lyt-2 and Thy-1 in cytolysis, we have generated, by ethyl methanesulfonate mutagenesis and selection, variants of the cloned cytolytic T lymphocyte line L3 that specifically lack either Lyt-2 or Thy-1. An analysis of these variants indicates that neither Lyt-2 nor Lyt-3 is responsible for the lethal hit, but suggests that Lyt-2 and/or Lyt-3 are required for an antigen receptor functional in cytolysis. The data also suggest that the expression of Lyt-3 on the cell surface is not independent of the expression of Lyt-2. Finally the data indicate the Thy-1 plays no role in cytolysis.


Sign in / Sign up

Export Citation Format

Share Document