DNA repair modifies the site and strand specificity of ethyl methanesulfonate mutagenesis in yeast

Mutagenesis ◽  
1992 ◽  
Vol 7 (6) ◽  
pp. 461-469 ◽  
Author(s):  
Bernard A. Kunz ◽  
Maila Gabriel ◽  
Xiaolin Kang ◽  
Susanne E. Kohalmi ◽  
Karin A. Terrick
Keyword(s):  
Dna Repair ◽  
Ethyl Methanesulfonate ◽  
Ethyl Methanesulfonate Mutagenesis

scholarly journals Lyt-2-/Lyt 3- variants of a cloned cytolytic T cell line lack an antigen receptor functional in cytolysis.

1981 ◽  
Vol 153 (3) ◽  
pp. 595-604 ◽  
Author(s):  
D P Dialynas ◽  
M R Loken ◽  
A L Glasebrook ◽  
F W Fitch
Keyword(s):  
T Cell ◽  
Cell Surface ◽  
Cell Line ◽  
T Lymphocyte ◽  
Antigen Receptor ◽  
Ethyl Methanesulfonate ◽  
Cytolytic T Lymphocyte ◽  
Ethyl Methanesulfonate Mutagenesis

To investigate the role of Lyt-2 and Thy-1 in cytolysis, we have generated, by ethyl methanesulfonate mutagenesis and selection, variants of the cloned cytolytic T lymphocyte line L3 that specifically lack either Lyt-2 or Thy-1. An analysis of these variants indicates that neither Lyt-2 nor Lyt-3 is responsible for the lethal hit, but suggests that Lyt-2 and/or Lyt-3 are required for an antigen receptor functional in cytolysis. The data also suggest that the expression of Lyt-3 on the cell surface is not independent of the expression of Lyt-2. Finally the data indicate the Thy-1 plays no role in cytolysis.

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