Sequence of the complete P protein gene and part of the M protein gene from the histidine transport operon of Escherichiacolicompared to that ofSalmonella typhimurium

SUMMARYStreptococcus pyogenescauses a variety of infections because of virulence factors such as capsular hyaluronic acid and M protein. The aim of this study was to determineemmtypes and capsule phenotype in 110 isolates ofS. pyogenesfrom patients with invasive (sterile sites) and non-invasive (mainly pharyngitis) infections in Chile, and the relationship between both virulence factors. The most abundant types found wereemm12,emm1,emm4andemm28and their distribution was similar to that seen in Latin America and developed countries, but very different from that in Asia and Pacific Island countries. Ten of 16emmtypes identified in pharyngeal isolates were found in sterile-site isolates, and three of nineemmtypes of sterile-site isolates occurred in pharyngeal isolates; threeemmsubtypes were novel. The amount of hyaluronic acid was significantly higher in sterile-site isolates but did not differ substantially amongemmtypes. Only three isolates were markedly capsulate and two of them had mutations in thecsrRgene that codes for a repressor of capsule synthesis genes. We found a non-random association betweenemmtypes andcsrRgene alleles suggesting that horizontal gene transfer is not freely occurring in the population.

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Genomic analysis of non-ketotic hyperglycinaemia: A partial deletion of P-protein gene

Journal of Inherited Metabolic Disease ◽

10.1007/bf01799584 ◽

1990 ◽

Vol 13 (5) ◽

pp. 766-770 ◽

Cited By ~ 3

Author(s):

K. Tada ◽

S. Kure ◽

A. Kume ◽

K. Hiraga

Keyword(s):

Protein Gene ◽

Genomic Analysis ◽

Partial Deletion ◽

P Protein

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Suppression of the Sendai Virus M Protein through a Novel Short Interfering RNA Approach Inhibits Viral Particle Production but Does Not Affect Viral RNA Synthesis

Journal of Virology ◽

10.1128/jvi.02291-06 ◽

2006 ◽

Vol 81 (6) ◽

pp. 2861-2868 ◽

Cited By ~ 25

Author(s):

Geneviève Mottet-Osman ◽

Frédéric Iseni ◽

Thierry Pelet ◽

Maciej Wiznerowicz ◽

Dominique Garcin ◽

...

Keyword(s):

Particle Production ◽

Protein Gene ◽

Fluorescent Protein ◽

Sendai Virus ◽

Virus Production ◽

Experimental System ◽

M Protein ◽

Green Fluorescent Protein Gene ◽

Target Sequence ◽

Interfering Rna

ABSTRACT Short RNA interference is more and more widely recognized as an effective method to specifically suppress viral functions in eukaryotic cells. Here, we used an experimental system that allows suppression of the Sendai virus (SeV) M protein by using a target sequence, derived from the green fluorescent protein gene, that was introduced in the 3′ untranslated region of the M protein mRNA. Silencing of the M protein gene was eventually achieved by a small interfering RNA (siRNA) directed against this target sequence. This siRNA was constitutively expressed in a cell line constructed by transduction with an appropriate lentivirus vector. Suppression of the M protein was sufficient to diminish virus production by 50- to 100-fold. This level of suppression had no apparent effect on viral replication and transcription, supporting the lack of M involvement in SeV transcription or replication control.

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