scholarly journals Prevalence of the M Protein Gene in Group C and Group G Streptococci Isolated from Patients in Thailand

2017 ◽  
Vol 70 (1) ◽  
pp. 108-110 ◽  
Author(s):  
Somporn Srifuengfung ◽  
Chanwit Tribuddharat ◽  
Suwandee Sapcharoen ◽  
Perapon Nitayanon
Keyword(s):  
Protein Gene ◽  
M Protein ◽  
Group G Streptococci

M protein gene typing of Streptococcus pyogenes by nonradioactively labeled oligonucleotide probes.

1992 ◽  
Vol 30 (9) ◽  
pp. 2391-2397 ◽  
Author(s):  
A Kaufhold ◽  
A Podbielski ◽  
D R Johnson ◽  
E L Kaplan ◽  
R Lütticken
Keyword(s):  
Streptococcus Pyogenes ◽  
Protein Gene ◽  
M Protein ◽  
Oligonucleotide Probes ◽  
Gene Typing

scholarly journals M or M-like protein gene polymorphisms in human group G streptococci.

1995 ◽  
Vol 33 (2) ◽  
pp. 356-363 ◽  
Author(s):  
N Schnitzler ◽  
A Podbielski ◽  
G Baumgarten ◽  
M Mignon ◽  
A Kaufhold
Keyword(s):  
Gene Polymorphisms ◽  
Protein Gene ◽  
Human Group ◽  
Group G Streptococci

M-protein gene-type distribution and hyaluronic acid capsule in group AStreptococcusclinical isolates in Chile: association ofemmgene markers withcsrRalleles

2011 ◽  
Vol 140 (7) ◽  
pp. 1286-1295 ◽  
Author(s):  
A. WOZNIAK ◽  
P. ROJAS ◽  
C. RODRÍGUEZ ◽  
A. UNDABARRENA ◽  
C. GARATE ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Virulence Factors ◽  
Protein Gene ◽  
Developed Countries ◽  
M Protein ◽  
Pacific Island ◽  
Non Invasive ◽  
Sterile Site ◽  
Hyaluronic Acid Capsule ◽  
The Relationship

SUMMARYStreptococcus pyogenescauses a variety of infections because of virulence factors such as capsular hyaluronic acid and M protein. The aim of this study was to determineemmtypes and capsule phenotype in 110 isolates ofS. pyogenesfrom patients with invasive (sterile sites) and non-invasive (mainly pharyngitis) infections in Chile, and the relationship between both virulence factors. The most abundant types found wereemm12,emm1,emm4andemm28and their distribution was similar to that seen in Latin America and developed countries, but very different from that in Asia and Pacific Island countries. Ten of 16emmtypes identified in pharyngeal isolates were found in sterile-site isolates, and three of nineemmtypes of sterile-site isolates occurred in pharyngeal isolates; threeemmsubtypes were novel. The amount of hyaluronic acid was significantly higher in sterile-site isolates but did not differ substantially amongemmtypes. Only three isolates were markedly capsulate and two of them had mutations in thecsrRgene that codes for a repressor of capsule synthesis genes. We found a non-random association betweenemmtypes andcsrRgene alleles suggesting that horizontal gene transfer is not freely occurring in the population.

scholarly journals Suppression of the Sendai Virus M Protein through a Novel Short Interfering RNA Approach Inhibits Viral Particle Production but Does Not Affect Viral RNA Synthesis

2006 ◽  
Vol 81 (6) ◽  
pp. 2861-2868 ◽  
Author(s):  
Geneviève Mottet-Osman ◽  
Frédéric Iseni ◽  
Thierry Pelet ◽  
Maciej Wiznerowicz ◽  
Dominique Garcin ◽  
...  
Keyword(s):  
Particle Production ◽  
Protein Gene ◽  
Fluorescent Protein ◽  
Sendai Virus ◽  
Virus Production ◽  
Experimental System ◽  
M Protein ◽  
Green Fluorescent Protein Gene ◽  
Target Sequence ◽  
Interfering Rna

ABSTRACT Short RNA interference is more and more widely recognized as an effective method to specifically suppress viral functions in eukaryotic cells. Here, we used an experimental system that allows suppression of the Sendai virus (SeV) M protein by using a target sequence, derived from the green fluorescent protein gene, that was introduced in the 3′ untranslated region of the M protein mRNA. Silencing of the M protein gene was eventually achieved by a small interfering RNA (siRNA) directed against this target sequence. This siRNA was constitutively expressed in a cell line constructed by transduction with an appropriate lentivirus vector. Suppression of the M protein was sufficient to diminish virus production by 50- to 100-fold. This level of suppression had no apparent effect on viral replication and transcription, supporting the lack of M involvement in SeV transcription or replication control.

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