scholarly journals Synthesis and biological activity of cyclic ADP-carbocyclic-ribose and its analogs as stable mimics of Ca2+-mobilizing second messenger cyclic ADP-ribose

2001 ◽  
Vol 1 (1) ◽  
pp. 5-6
Author(s):  
S. Shuto ◽  
T. Kudoh ◽  
M. Fukuoka ◽  
Y. Ueno ◽  
A. Matsuda
Keyword(s):  
Biological Activity ◽  
Second Messenger ◽  
Cyclic Adp Ribose

scholarly journals A Minimal Structural Analogue of Cyclic ADP-ribose

2005 ◽  
Vol 280 (16) ◽  
pp. 15952-15959 ◽  
Author(s):  
Andreas H. Guse ◽  
Xianfeng Gu ◽  
Liangren Zhang ◽  
Karin Weber ◽  
Elisabeth Krämer ◽  
...  
Keyword(s):  
Biological Activity ◽  
T Lymphocytes ◽  
Cardiac Myocytes ◽  
Second Messenger ◽  
Cell Types ◽  
Structural Analogue ◽  
Cyclic Adp Ribose

Cyclic ADP-ribose (cADPR) is an endogenous Ca2+-mobilizing second messenger in many cell types and organisms. Although the biological activity of several modified analogues of cADPR has been analyzed, most of these structures were still very similar to the original molecule. Recently, we have introduced simplified analogues in which the northern ribose (N1-linked ribose) was replaced by an ether strand (Gu, X., Yang, Z., Zhang, L., Kunerth, S., Fliegert, R., Weber, K., Guse, A. H., and Zhang, L. (2004)J. Med. Chem.47, 5674–5682). Here we also demonstrate that the southern ribose (N9-linked ribose) can be replaced by an ether strand resulting inN1-[(phosphoryl-O-ethoxy)-methyl]-N9-[(phosphoryl-O-ethoxy)-methyl]-hypoxanthinecyclic pyrophosphate (cIDP-DE). This minimal structural analogue of cyclic ADP-ribose released Ca2+from intracellular stores of permeabilized Jurkat T lymphocytes. In intact T lymphocytes initial subcellular Ca2+release events, global Ca2+release, and subsequent global Ca2+entry were observed. Cardiac myocytes freshly prepared from mice responded to cIDP-DE by increased recruitment of localized Ca2+signals and by global Ca2+waves.

Role of cyclic ADP-ribose in the regulation of [Ca2+]i in porcine tracheal smooth muscle

1998 ◽  
Vol 274 (6) ◽  
pp. C1653-C1660 ◽  
Author(s):  
Y. S. Prakash ◽  
Mathur S. Kannan ◽  
Timothy F. Walseth ◽  
Gary C. Sieck
Keyword(s):  
Smooth Muscle ◽  
Second Messenger ◽  
Ruthenium Red ◽  
Tracheal Smooth Muscle ◽  
Cyclic Adp Ribose ◽  
Intracellular Ca ◽  
Subsequent Exposure ◽  
Trisphosphate Receptor ◽  
Dose Dependent Increase

The purpose of the present study was to determine whether cyclic ADP-ribose (cADPR) acts as a second messenger for Ca2+ release through ryanodine receptor (RyR) channels in tracheal smooth muscle (TSM). Freshly dissociated porcine TSM cells were permeabilized with β-escin, and real-time confocal microscopy was used to examine changes in intracellular Ca2+ concentration ([Ca2+]i). cADPR (10 nM–10 μM) induced a dose-dependent increase in [Ca2+]i, which was blocked by the cADPR receptor antagonist 8-amino-cADPR (20 μM) and by the RyR blockers ruthenium red (10 μM) and ryanodine (10 μM), but not by the inositol 1,4,5-trisphosphate receptor blocker heparin (0.5 mg/ml). During steady-state [Ca2+]ioscillations induced by acetylcholine (ACh), addition of 100 nM and 1 μM cADPR increased oscillation frequency and decreased peak-to-trough amplitude. ACh-induced [Ca2+]ioscillations were blocked by 8-amino-cADPR; however, 8-amino-cADPR did not block the [Ca2+]iresponse to a subsequent exposure to caffeine. These results indicate that cADPR acts as a second messenger for Ca2+ release through RyR channels in TSM cells and may be necessary for initiating ACh-induced [Ca2+]ioscillations.

A comparison of the biological activities of authentic rat GRF(1–43)OH with the analogue rat GRF(1–29)NH2

1991 ◽  
Vol 69 (2) ◽  
pp. 181-184 ◽  
Author(s):  
J. Kraicer ◽  
M. B. French ◽  
B. T. Lussier ◽  
B. C. Moor ◽  
P. Brazeau
Keyword(s):  
Growth Hormone ◽  
Biological Activity ◽  
Biological Activities ◽  
Second Messenger ◽  
Growth Hormone Releasing Factor ◽  
Response Characteristics ◽  
Rat Growth ◽  
Prior Administration ◽  
Perifusion System ◽  
Subsequent Administration

The purpose of this study was to characterize the biological activity of the synthetic rat growth hormone releasing factor analogue rGRF(1–29)NH2 and to compare its action on growth hormone (GH) release to that of authentic rGRF(1–43)OH. We first compared the concentration–response characteristics of the two peptides in static incubation, and then examined the reversibility and repeatability of the GH response in a perifusion system. Authentic rGRF(1–43)OH was significantly more potent in static incubation (EC50 = 3 × 10−11 M) than the analogue (5 × 10−11 M), whereas the reverse held true in perifusion. The shapes of the GH responses were similar for both peptides in the perifusion system. However, while the GH response to authentic rGRF was repeatable, the prior administration of rGRF(1–29)NH2 significantly reduced (>50%) the GH response to the subsequent administration of either rGRF(1–29)NH2 or rGRF(1–43)OH. Thus authentic rGRF and the synthetic fragment may have different actions at the level of the GRF receptor or at a postreceptor (second messenger) step.Key words: growth hormone, growth hormone releasing factor, adenohypophysis.

scholarly journals Abscisic Acid Is an Endogenous Stimulator of Insulin Release from Human Pancreatic Islets with Cyclic ADP Ribose as Second Messenger

2008 ◽  
Vol 283 (47) ◽  
pp. 32188-32197 ◽  
Author(s):  
Santina Bruzzone ◽  
Nicoletta Bodrato ◽  
Cesare Usai ◽  
Lucrezia Guida ◽  
Iliana Moreschi ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Pancreatic Islets ◽  
Insulin Release ◽  
Second Messenger ◽  
Human Pancreatic Islets ◽  
Cyclic Adp Ribose

scholarly journals Cyclic ADP-ribose as a potential second messenger for neuronal Ca2+signaling

2001 ◽  
Vol 76 (2) ◽  
pp. 321-331 ◽  
Author(s):  
Haruhiro Higashida ◽  
Minako Hashii ◽  
Shigeru Yokoyama ◽  
Naoto Hoshi ◽  
Kiyofumi Asai ◽  
...  
Keyword(s):  
Second Messenger ◽  
Cyclic Adp Ribose
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