scholarly journals Plasma or whole blood concentrations of trace elements in patients treated by haemodiafiltration with on-line prepared substitution fluid

1998 ◽  
Vol 13 (90005) ◽  
pp. 29-33 ◽  
Author(s):  
G Bonforte
Keyword(s):  
Trace Elements ◽  
Whole Blood ◽  
Blood Concentrations ◽  
On Line

scholarly journals Serum Vitamin D Components and Whole Blood Trace Elements Storage in Chinese Short Stature Children

2020 ◽  
Author(s):  
Bei Xu ◽  
Yue Feng ◽  
Lingling Gan ◽  
Yamei Zhang ◽  
Wenqiang Jiang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Trace Elements ◽  
Nutritional Status ◽  
Short Stature ◽  
Whole Blood ◽  
Healthy Subjects ◽  
Serum Vitamin ◽  
Serum Levels ◽  
Healthy Controls ◽  
Blood Concentrations

Abstract Background: Literature data on nutritional status in short stature children are unfortunately still scarce. The aim of the study was to compare the serum levels of 25(OH)D2, 25(OH)D3, total 25(OH)D and whole blood trace elements between short stature and healthy controls in Chinese children.Methods: A case-control study including 370 short stature (SS) children and 398 healthy controls (HC) was performed in Mianyang Central Hospital from January 2017 to June 2020. Serum 25(OH)D2 and 25(OH)D3 were accurately measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and were summed to obtain total 25(OH)D. Whole blood concentrations of calcium (Ca), magnesium (Mg), copper (Cu), zinc (Zn), iron (Fe), manganese (Mn), selenium (Se) and lead (Pb) were analyzed by inductively coupled plasma mass spectrometry (ICP-MS) method. Results: 25(OH)D2 and total 25(OH)D levels in the SS group were significantly lower than the HC subjects (both P<0.05). Besides, both 25(OH)D2 and 25(OH)D3 were positively correlated with total 25(OH)D in the SS and HC groups. 25(OH)D2 levels had weakly negative association with 25(OH)D3 among healthy subjects, whereas no correlation between 25(OH)D2 and 25(OH)D3 levels was found in short stature group. Otherwise, a significant elevation was observed in Zn (P<0.001), Fe (P<0.001), and Se (P=0.027) in SS patients, while a statistically significant decrease in Cu (P=0.002) and Mn (P<0.001) was found. Additionally, the significant correlation of serum levels of 25(OH)D2, 25(OH)D3, total 25(OH)D and trace elements were observed between SS and HC group.Conclusion: SS children had more severe deficiency of 25(OH)D2 and total 25(OH)D than healthy subjects. In addition, deficiency of Cu and Mn, but elevation of Zn, Fe and Se were observed. Achieved information about vitamin D and trace element status in SS subjects could further improve the nutritional status of these patient groups.

scholarly journals Real-time Monitoring of Propofol in Expired Air in Humans Undergoing Total Intravenous Anesthesia

2007 ◽  
Vol 106 (4) ◽  
pp. 665-674 ◽  
Author(s):  
Cyrill Hornuss ◽  
Siegfried Praun ◽  
Johannes Villinger ◽  
Albert Dornauer ◽  
Patrick Moehnle ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Real Time ◽  
Whole Blood ◽  
Total Intravenous Anesthesia ◽  
Real Time Monitoring ◽  
Intravenous Anesthesia ◽  
Blood Concentrations ◽  
Arterial Blood ◽  
On Line ◽  
Mass Spectrometry System

Background The physicochemical properties of propofol could allow diffusion across the alveolocapillary membrane and a measurable degree of pulmonary propofol elimination. The authors tested this hypothesis and showed that propofol can be quantified in expiratory air and that propofol breath concentrations reflect blood concentrations. This could allow real-time monitoring of relative changes in the propofol concentration in arterial blood during total intravenous anesthesia. Methods The authors measured gas-phase propofol using a mass spectrometry system based on ion-molecule reactions coupled with quadrupole mass spectrometry which provides a highly sensitive method for on-line and off-line measurements of organic and inorganic compounds in gases. In a first sequence of experiments, the authors sampled blood from neurosurgery patients undergoing total intravenous anesthesia and performed propofol headspace determination above the blood sample using an auto-sampler connected to the mass spectrometry system. In a second set of experiments, the mass spectrometry system was connected directly to neurosurgery patients undergoing target-controlled infusion via a T piece inserted between the endotracheal tube and the Y connector of the anesthesia machine, and end-expiratory propofol concentrations were measured on-line. Results A close correlation between propofol whole blood concentration and propofol headspace was found (range of Pearson r, 0.846-0.957; P &lt; 0.01; n = 6). End-expiratory propofol signals mirrored whole blood values with close intraindividual correlations between both parameters (range of Pearson r, 0.784-0.985; n = 11). Conclusion Ion-molecule reaction mass spectrometry may allow the continuous and noninvasive monitoring of expiratory propofol levels in patients undergoing general anesthesia.

Biomonitoring of 29 trace elements in whole blood from inhabitants of Cotonou (Benin) by ICP-MS

2017 ◽  
Vol 43 ◽  
pp. 38-45 ◽  
Author(s):  
Brice Yedomon ◽  
Alain Menudier ◽  
Florence Lecavelier Des Etangs ◽  
Ludovic Anani ◽  
Benjamin Fayomi ◽  
...  
Keyword(s):  
Trace Elements ◽  
Whole Blood ◽  
Icp Ms

scholarly journals Liquid Chromatography Tandem Mass Spectrometric Analytical Method for Study of Colchicine in Rats Given Low Doses

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 2007
Author(s):  
Hamdah M. Al Nebaihi ◽  
Tyson S. Le ◽  
Neal M. Davies ◽  
Dion R. Brocks
Keyword(s):  
Whole Blood ◽  
Multiple Reaction Monitoring ◽  
Internal Standard ◽  
Rat Plasma ◽  
Low Doses ◽  
Blood Concentrations ◽  
Liquid Chromatography Tandem Mass ◽  
Sensitivity And Selectivity ◽  
Tandem Mass Spectrometric ◽  
Lower Limits

A selective and sensitive assay was developed for colchicine in rat specimens. Colchicine and its deuterated analog (as internal standard, IS) were extracted from rat specimens (minimal 0.1 mL plasma, whole blood, or urine) using liquid-liquid extraction with n-hexane:dichloromethane:isopropanol. The mobile phase (formic acid: ammonium acetate: methanol) was pumped with uniform flow through an octadecylsilane analytical column. Detection was carried out by electrospray positive ionization in the multiple-reaction monitoring mode. The assay (total run time <3 min) had excellent linearity over a wide (400–800-fold) concentration range. The mean absolute recovery was >96.8%. The intra- and inter-day coefficients of variation were ˂15%, with lower limits of quantitation of 0.5 ng/mL in 0.1 mL of rat plasma. The method also provided the same lower limits of quantitation in urine and whole blood with 0.1 mL volumes, and 0.1 ng/mL using 0.5 mL of rat plasma. The blood-to-plasma ratio was >1. Rats had measurable colchicine blood concentrations for at least 24 h after intravenous doses of 0.1 mg/kg. The method possessed suitable measures of sensitivity and selectivity for detecting colchicine in several specimen types in rats given low doses.

scholarly journals CHANGES OF NONESSENTIAL TRACE ELEMENTS LEVELS IN WHOLE BLOOD OF END STAGE RENAL DISEASE ADULT PATIENTS

2017 ◽  
pp. 114-125
Author(s):  
O. O. Makarov ◽  
E. O. Pisarev ◽  
B. S. Sheiman ◽  
M. V. Kulizkiy ◽  
D. V. Peretyatko ◽  
...  
Keyword(s):  
Trace Elements ◽  
Whole Blood ◽  
Chronic Kidney Disease Stage ◽  
Disease Stage ◽  
Blood Levels ◽  
Healthy Donors ◽  
Plasma Mass Spectrometry ◽  
Stage Renal Disease ◽  
End Stage ◽  
Esrd Patients

Aims: Accumulation of trace elements occurs in conditions of decreased or termination of kidneys functions. In some conditions increased trace elements can obtain toxic features. On other hand, researches are showing that concentration of some trace elements could be decreased in ERSD patients, too. The most important factor affecting trace element concentration in ERSD patient is the degree of renal failure and using of replacement therapy. Materials and methods: We determined the concentration of microelements (beryllium, boron, aluminum, vanadium, chromium, nickel, arsenic, rubidium, strontium, cadmium, cesium, barium, thallium and lead) in whole blood of 41 ESRD patients with chronic kidney disease stage VD, who were treated with hemodialysis / hemodiafiltration and in 61 conditionally healthy donors. Determination of whole bloods trace elements content was conducted using inductive coupled plasma mass- spectrometry (ICPMS).  Results: It is determinate that levels of beryllium, boron, aluminum, vanadium, chromium, strontium, cadmium, barium, thallium and lead is reliable increased in ESRD patients. Decreased levels are observed for nickel, arsenic, cesium and rubidium. Conclusions: ESRD is accompanied with substantial and multidirectional changes of trace elements blood levels. During researches has shown that in ESRD patients processes of trace elements accumulation are prevailing over elimination ones.

Δ9-THC, 11-OH-Δ9-THC and Δ9-THCCOOH plasma or serum to whole blood concentrations distribution ratios in blood samples taken from living and dead people

2001 ◽  
Vol 123 (2-3) ◽  
pp. 159-164 ◽  
Author(s):  
Christian Giroud ◽  
Annick Ménétrey ◽  
Marc Augsburger ◽  
Thierry Buclin ◽  
Pablo Sanchez-Mazas ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood Concentrations ◽  
Blood Samples

scholarly journals Trace Element Analysis of Cancerous and Non-cancerous Breast Tissues of African Women in Southwest Nigeria Using Particle-Induced X-ray Emission Technique

2019 ◽  
Vol 13 ◽  
pp. 117822341984069
Author(s):  
David O Olaiya ◽  
Olusegun I Alatise ◽  
Oyebamiji O Oketayo ◽  
Olawale E Abiye ◽  
Eusebius I Obianjunwa ◽  
...  
Keyword(s):  
Trace Elements ◽  
Whole Blood ◽  
Trace Element Analysis ◽  
African Women ◽  
Animal Blood ◽  
X Ray ◽  
Freeze Dried ◽  
Proton Beam Energy ◽  
Southwest Nigeria ◽  
Breast Tissues

In this study, we applied particle-induced X-ray emission (PIXE) spectroscopy to investigate the levels of trace elements in breast tissues and whole blood (cancerous and non-cancerous) of selected African women in Ile-Ife, Southwest Nigeria. Freeze-dried and homogenized specimens obtained through mastectomy from clinically diagnosed patients were made into 11-mm-diameter pellets. The pellets were irradiated with 2.5 MeV proton beam energy from a 1.7 MV 5SDH Tandem accelerator. The PIXE analytical system was calibrated with certified reference matrices of Bovine Liver and Animal Blood: NIST 1577a and IAEA-A-13, respectively. A total of 23 elements: Na, K, Ca, Cl, S, Al, P, Si, Zn, Pb, Br, Rb, Zr, Se, Sr, Mn, V, Ti, Cu, Fe, Ni, Cr, and Mg were detected. The results indicated that the levels were within 0.9-5288 and 0.6-2320 ppm in breast tissues and 0.3-17228 and 2.0-2475 ppm in the whole blood of cancerous and non-cancerous subjects, respectively. At the .05 level of significance, significant differences exist between these levels in the cancerous and non-cancerous breast tissues ( t = 0.008) as well as the whole blood ( t = 0.041). The results gave the baseline concentration of the observed trace elements in the normal and malignant subjects and indicated PIXE as a powerful tool for such investigation.

Radioreceptor Assay for Quantifying FK-506 Immunosuppressant in Whole Blood

1992 ◽  
Vol 38 (7) ◽  
pp. 1307-1310 ◽  
Author(s):  
J N Murthy ◽  
Y Chen ◽  
V S Warty ◽  
R Venkataramanan ◽  
J G Donnelly ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Enzyme Immunoassay ◽  
Whole Blood ◽  
Binding Protein ◽  
Radioreceptor Assay ◽  
Fk 506 ◽  
Blood Concentrations ◽  
Chromatographic Columns ◽  
Analytical Recovery

Abstract We describe a quantitative radioreceptor assay (RRA) for quantifying FK-506 in whole blood. FK-506 extracted from whole blood with a cyclohexyl-sorbent column competes with [3H]dihydro-FK-506 for binding to a partially purified preparation of FK-506 binding protein (FK-BP). Free and protein-bound FK-506 are separated on LH 20 Sephadex chromatographic columns. We compared the results of this method with those of an enzyme immunoassay that uses a monoclonal antibody: r = 0.97, Sy/x = 0.039. Between-day precisions (CV) at FK-506 concentrations of 8 and 17 micrograms/L were 9.2% and 8.2%, respectively. Within-run precisions were 5.9%, 8.1%, and 9.4%, respectively, at 4, 8, and 15 micrograms/L. Analytical recovery, evaluated at 5, 10, 15, 20, and 25 micrograms/L for FK-506 added to whole blood, ranged from 98% to 103%. The assay can reliably quantify FK-506 blood concentrations between 1.0 and 25 micrograms/L.

Trace Element Status (Zinc, Copper, Selenium, Iron, Manganese) in Patients with Long-Term Home Parenteral Nutrition

2016 ◽  
Vol 69 (2) ◽  
pp. 120-124 ◽  
Author(s):  
Milan Dastych Jr. ◽  
Michal Šenkyřík ◽  
Milan Dastych ◽  
František Novák ◽  
Petr Wohl ◽  
...  
Keyword(s):  
Trace Elements ◽  
Parenteral Nutrition ◽  
Blood Plasma ◽  
Whole Blood ◽  
Statistical Significance ◽  
Home Parenteral Nutrition ◽  
Control Group ◽  
The Status ◽  
In Blood Plasma

Background: The objective of the present study was to determine concentrations of zinc (Zn), copper (Cu), iron (Fe), selenium (Se) in blood plasma and manganese (Mn) in the whole blood in patients with long-term home parenteral nutrition (HPN) in comparison to the control group. Patients and Methods: We examined 68 patients (16 men and 52 women) aged from 28 to 68 years on a long-term HPN lasting from 4 to 96 months. The short bowel syndrome was an indication for HPN. The daily doses of Zn, Cu, Fe, Se and Mn in the last 3 months were determined. Results: No significant differences in blood plasma were found for Zn, Cu and Fe in patients with HPN and in the control group (p > 0.05). The concentration of Mn in whole blood was significantly increased in HPN patients (p < 0.0001), while Se concentration in these patients was significantly decreased (p < 0.005). The concentration of Mn in the whole blood of 16 patients with cholestasis was significantly increased compared to the patients without cholestasis (p < 0.001). The Cu concentration was increased with no statistical significance. Conclusion: In long-term HPN, the status of trace elements in the patients has to be continually monitored and the daily substitution doses of these elements have to be flexibly adjusted. Dosing schedule needs to be adjusted especially in cases of cholestatic hepatopathy. A discussion about the optimal daily dose of Mn in patients on HPN is appropriate. For clinical practice, the availability of a substitution mixture of trace elements lacking Mn would be advantageous.

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