Effects of in-vitro exposure to HCG on subsequent HCC-responsiveness of human granulosa cells obtained following treatment with GnRH analogue and gonadotrophins: an in-vitro model for luteal phase support

1991 ◽  
Vol 6 (2) ◽  
pp. 198-202 ◽  
Author(s):  
D.H. Edgar ◽  
K.M. Whalley ◽  
J.A. Gemmell ◽  
G.B. James ◽  
J.A. Mills
Keyword(s):  
Granulosa Cells ◽  
Luteal Phase ◽  
In Vitro Model ◽  
Gnrh Analogue ◽  
Luteal Phase Support ◽  
Human Granulosa Cells ◽  
In Vitro Exposure ◽  
Vitro Model

Gonadotrophic regulation of prolactin mediated progesterone secretion in vitro

1985 ◽  
Vol 110 (2) ◽  
pp. 251-256 ◽  
Author(s):  
J. Steven Alexander ◽  
Thomas M. Crisp
Keyword(s):  
Granulosa Cells ◽  
In Vitro Model ◽  
Fold Increase ◽  
Model System ◽  
Regulatory Mechanisms ◽  
Progesterone Secretion ◽  
Vitro Model ◽  
Dose Dependent ◽  
Cells Cultured

Abstract. The effects of preincubating rat granulosa cells with FSH, LH, and Prl on subsequent Prl mediated progesterone secretion were investigated. Granulosa cells were isolated from ovarian follicles 50 h after injection of 5 IU PMSG and were then plated on poly-l-lysine coated coverslips in serum supplemented medium. Cells were preincubated for 24 h in the absence of hormones (control) or with the addition of either 0.25, 2.5, 25 ng/ml rat FSH or rat LH, or 1 μg/ml rat Prl. Following the preincubation period, cells were maintained for an additional 6 or 8 days in the presence or absence of 1 μg/ml Prl. When cells were preincubated with FSH or LH, only the two higher concentrations (2.5 and 25 ng/ml) stimulated significantly more progesterone secretion than control cultures during the 24 h preincubation period. For each series of preincubations, cells cultured for 6 or 8 days in the presence of Prl secreted significantly more progesterone at each day of culture than cells cultured without Prl. Cells preincubated and cultured with Prl secreted only 3–7-fold more progesterone than cells preincubated in control medium and then cultured with Prl. Preincubation with FSH or LH promoted a 20–45-fold increase in Prl mediated progesterone secretion compared to control preincubation cultures that also subsequently were cultured with Prl. The magnitude of Prl mediated progesterone secretion observed through 6 days of culturing was dose dependent on the preincubation concentration of FSH or LH. The establishment of an in vitro model system in which gonadotrophins enhance the responsiveness of granulosa cells to Prl in serum supplemented medium provides the opportunity for study of the regulatory mechanisms involved with the induction and maintenance of such responsiveness.

Analysis of granulosa cells by single cell PCR—establishment of an in vitro model system

2011 ◽  
Vol 90 (2) ◽  
pp. 176
Author(s):  
J. Pastuschek ◽  
S. Hoelters ◽  
S. Neubeck ◽  
J.S. Fitzgerald ◽  
E. Schleussner ◽  
...  
Keyword(s):  
Single Cell ◽  
Granulosa Cells ◽  
In Vitro Model ◽  
Model System ◽  
Single Cell Pcr ◽  
In Vitro Model System ◽  
Vitro Model

Purinergic signalling in rat GFSHR-17 granulosa cells: an in vitro model of granulosa cells in maturing follicles

2009 ◽  
Vol 41 (1) ◽  
pp. 85-94 ◽  
Author(s):  
Willem Bintig ◽  
Judith Baumgart ◽  
Wilhelm J. Walter ◽  
Alexander Heisterkamp ◽  
Holger Lubatschowski ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
In Vitro Model ◽  
Purinergic Signalling ◽  
Vitro Model

Spheroids of granulosa cells provide an in vitro model for programmed cell death coupled to steroidogenesis

2009 ◽  
Vol 77 (1) ◽  
pp. 60-69 ◽  
Author(s):  
Katja Hummitzsch ◽  
Albert M. Ricken ◽  
Daniel Kloß ◽  
Sabine Erdmann ◽  
Marcin S. Nowicki ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Granulosa Cells ◽  
In Vitro Model ◽  
Vitro Model

Human granulosa cells in vitro exposure to bisphenol a: FTIR microspectroscopy and molecular evidences

2015 ◽  
Vol 104 (3) ◽  
pp. e141
Author(s):  
G. Gioacchini ◽  
E. Giorgini ◽  
L. Vaccari ◽  
E. Sereni ◽  
C. Zaca ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Granulosa Cells ◽  
Human Granulosa Cells ◽  
Ftir Microspectroscopy ◽  
In Vitro Exposure

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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