Optical and Electron-microscopic Study of Immunologically Competent Cells During the Reaction of Graft Against the Host Using a standard microscope and an electron microscope

Various methods of dissection, fixation, osmication, sectioning and staining were tested in order to develop an acceptable technique for preparing 9, 10, 11, and 12-day-old rat otocysts for electron microscopic study. The general problems associated with embryonic tissue — Difficult handling, high water content and poor stainability are discussed, and concrete methods of preparation which significantly decrease these difficulties are proposed. The specific fixation and sectioning requirements of rat otocyst are also described in the elaboration of a method which will be used in subsequent studies of the organogenesis of rodent ear.

Download Full-text

Electron Microscopic Study on Rat Brown Adipose Tissue. Possible Sites of Glucose Turnover in Rat Brown Adipose Cells as shown by Electron Microscope Radioautography after Administration of Glucose-H3.

Journal of Electron Microscopy ◽

10.1093/oxfordjournals.jmicro.a049559 ◽

1967 ◽

Keyword(s):

Adipose Tissue ◽

Electron Microscope ◽

Brown Adipose Tissue ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Glucose Turnover ◽

Electron Microscopic ◽

Brown Adipose ◽

Adipose Cells

Download Full-text

An Electron Microscopic Study of the Changes in Platelets during Viscous Metamorphosis

Blood ◽

10.1182/blood.v20.5.566.566 ◽

1962 ◽

Vol 20 (5) ◽

pp. 566-580 ◽

Cited By ~ 22

Author(s):

P. A. CASTALDI ◽

B. G. FIRKIN ◽

P. M. BLACKWELL ◽

K. I. CLIFFORD

Keyword(s):

Electron Microscope ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Light Microscope ◽

Phase Contrast ◽

Intact Cell ◽

Complete Loss ◽

Electron Microscopic ◽

Thin Sections ◽

Sectioned Material

Abstract Viscous metamorphosis of platelets has been studied with the light microscope, and ultra-thin sections have been prepared at progressive stages for examination in the electron microscope. The phase contrast light microscope reveals rapid aggregation and distortion of platelets and gives the impression of their fusion into structureless aggregates during viscous metamorphosis. Sectioned material collected during viscous metamorphosis of platelets and examined in the electron microscope reveals a remarkable degree of retention of structure in a majority of the platelets. All become deficient in granules and devoid of vesicular spaces, but most retain intact cell membranes, and the structureless masses seen with the light microscope are found to consist of densely aggregated platelets. Fusion and complete loss of identity occurs in the minority. The retracted clot was found to contain densely aggregated, distorted and elongated platelets, empty of granules and intimately related to fibrin particles.

Download Full-text

THE ROLE OF THE GOLGI COMPLEX IN FAT ABSORPTION AS STUDIED WITH THE ELECTRON MICROSCOPE WITH OBSERVATIONS ON THE CYTOLOGY OF DUODENAL ABSORPTIVE CELLS

Journal of Experimental Medicine ◽

10.1084/jem.102.6.775 ◽

1955 ◽

Vol 102 (6) ◽

pp. 775-782 ◽

Cited By ~ 67

Author(s):

Jules M. Weiss ◽

Keyword(s):

Electron Microscope ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Golgi Complex ◽

Fat Absorption ◽

Electron Microscopic ◽

Absorptive Cells

An electron microscopic study of the morphology of fat absorption in the duodenum of normal and fat-fed mice supports the view that all absorbed fat passes through the cytoplasm of absorptive cells. The Golgi complex plays an important role in the transport of fat across these cells.

Download Full-text

The effect of EDTA, cations, and various buffers on the morphology of erythrocyte membranes: an electron-microscopic study

Blood ◽

10.1182/blood.v45.5.709.709 ◽

1975 ◽

Vol 45 (5) ◽

pp. 709-724 ◽

Cited By ~ 16

Author(s):

L Pinteric ◽

JF Manery ◽

IH Chaudry ◽

G Madapallimattam

Keyword(s):

Fine Structure ◽

Electron Microscope ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Ethylenediaminetetraacetic Acid ◽

Human Erythrocytes ◽

Erythrocyte Membranes ◽

Electron Microscopic ◽

Osmotic Hemolysis ◽

Ionic Calcium

Abstract Membranes of human erythrocytes were prepared by stepwise osmotic hemolysis in Ca2+-free solutions. Examination with the electron microscope after negative staining showed some short, conelike protuberances on the surface of about 20 percent of the ghosts, while 80 percent were round, intact spheres. After Ca2+ treatment, all membranes were round and intact. After exposure to ethylenediaminetetraacetic acid (EDTA) (1.0 mM, pH 7.4), the entire ghost surface was covered with long, thin extrusions called stromalytic forms (about 460 per cell). Their sizes, shapes, and fine structure are described. Exposure to ionic calcium (1.4 times 10-minus 4M) abolished the EDTA-induced stromalytic forms. A second exposure to EDTA reversed this Ca2+ effect. ATP, like EDTA, produced stromalytic forms. EDTA- induced stromalytic forms were also abolished by Zn2+, La3+, and Nd3+ at concentrations of 1–5 times 10-minus 4 M. Mg2+ at 10-minus 2 M was ineffective. Ghosts were prepared by graded lysis in various buffers. Those prepared in phosphate were the most stable and provided consistent EDTA effects and Ca2+ reversal. Ghosts in Tris-HCl showed breakdown unless salt was added. Moderately satisfactory ghosts were also obtained in Hepes-NaOH buffer and salt.

Download Full-text

A scanning electron microscopic study of epicuticular waxes of glumesin Avena magna, A. murphyi, and A. sterilis

Canadian Journal of Botany ◽

10.1139/b73-305 ◽

1973 ◽

Vol 51 (12) ◽

pp. 2381-2383 ◽

Cited By ~ 3

Author(s):

B. R. Baum ◽

V. E. Hadland

Keyword(s):

Electron Microscope ◽

Scanning Electron Microscope ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Epicuticular Waxes ◽

Electron Microscopic ◽

Avena Species ◽

Scanning Electron Microscopic Study ◽

Scanning Electron Microscopic ◽

Scanning Electron

The ultrastructure of epicuticular glume waxes in two tetraploid Avena species, A. magna and A. murphyi, and in one hexaploid species, A. sterilis, has been studied and documented with the aid of the scanning electron microscope (SEM). The usefulness of this approach for taxonomy and diagnostic purposes has been evaluated, and the specific configurations of those epicuticular waxes compared in relation to the genomes.

Download Full-text

Brief Report: Nuclear Bodies of Normal and Pathological Human Lymph Node Cells: An Electron Microscopic Study

Blood ◽

10.1182/blood.v29.2.269.269 ◽

1967 ◽

Vol 29 (2) ◽

pp. 269-275 ◽

Cited By ~ 23

Author(s):

ROBERT E. BROOKS ◽

BENJAMIN V. SIEGEL

Keyword(s):

Electron Microscope ◽

Lymph Node ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Nuclear Bodies ◽

Electron Microscopic ◽

Dense Granules ◽

Fibrillar Material ◽

Human Lymph Node ◽

Lymph Node Cells

Abstract Nuclear bodies in normal and pathologic human lymph node cells have been examined with the electron microscope and their structure has been illustrated and described. In normal lymph node cells, nuclear bodies are 0.3-0.5 microns in diameter, are slightly less electron dense than the nucleolus, and consist of peripheral fibrillar material with centrally located, dense granules, 200-400 Å in diameter. Morphologically abnormal nuclear bodies have been observed in a case of Hodgkin’s disease. The appearance of these atypical bodies would suggest either contact and fusion of two or more atypical bodies, or possibly the existence of single, large, irregular bodies.

Download Full-text

SHADOW-CASTING WITH HALIDES IN THE ELECTRON MICROSCOPE

Canadian Journal of Physics ◽

10.1139/p51-053 ◽

1951 ◽

Vol 29 (6) ◽

pp. 491-494 ◽

Cited By ~ 2

Author(s):

William H. Barnes ◽

Margaret S. Lambe

Keyword(s):

Electron Microscopy ◽

Electron Microscope ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Convenient Method ◽

Calcium Fluoride ◽

Electron Microscopic ◽

Shadow Casting

Experiments on the behavior of halides as shadow-casting agents in electron microscopy are described. The use of calcium fluoride, placed directly on the specimen screen, is suggested as a convenient method for routine qualitative shadowing of preparations in the preliminary stages of an electron microscopic study. No vacuum coating equipment is required.

Download Full-text

Electron Microscopic Study of Carbon Powders and Their Composites

Bulletin of Science and Practice ◽

10.33619/2414-2948/52/05 ◽

2020 ◽

Vol 6 (3) ◽

pp. 44-48

Author(s):

N. Zhogashtiev

Keyword(s):

Heat Treatment ◽

Electron Microscope ◽

Scanning Electron Microscope ◽

Microscopic Study ◽

Supercritical Fluid ◽

Electron Microscopic Study ◽

Raw Materials ◽

Sol Gel ◽

Electron Microscopic ◽

Scanning Electron

Primary ultrafine carbon powders were obtained using the sol-gel method and drying in a supercritical fluid using various carbon raw materials. By heat treatment at 1000–1100 °C, ultrafine carbon powders were obtained. The properties of primary ultrafine carbon powders and their annealing products were studied using a Tescan Vega 3 SEM scanning electron microscope (SEM). The results show that the starting materials affect the structure of the resulting ultrafine carbon powders.

Download Full-text

The effect of EDTA, cations, and various buffers on the morphology of erythrocyte membranes: an electron-microscopic study

Blood ◽

10.1182/blood.v45.5.709.bloodjournal455709 ◽

1975 ◽

Vol 45 (5) ◽

pp. 709-724

Author(s):

L Pinteric ◽

JF Manery ◽

IH Chaudry ◽

G Madapallimattam

Keyword(s):

Fine Structure ◽

Electron Microscope ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Ethylenediaminetetraacetic Acid ◽

Human Erythrocytes ◽

Erythrocyte Membranes ◽

Electron Microscopic ◽

Osmotic Hemolysis ◽

Ionic Calcium

Membranes of human erythrocytes were prepared by stepwise osmotic hemolysis in Ca2+-free solutions. Examination with the electron microscope after negative staining showed some short, conelike protuberances on the surface of about 20 percent of the ghosts, while 80 percent were round, intact spheres. After Ca2+ treatment, all membranes were round and intact. After exposure to ethylenediaminetetraacetic acid (EDTA) (1.0 mM, pH 7.4), the entire ghost surface was covered with long, thin extrusions called stromalytic forms (about 460 per cell). Their sizes, shapes, and fine structure are described. Exposure to ionic calcium (1.4 times 10-minus 4M) abolished the EDTA-induced stromalytic forms. A second exposure to EDTA reversed this Ca2+ effect. ATP, like EDTA, produced stromalytic forms. EDTA- induced stromalytic forms were also abolished by Zn2+, La3+, and Nd3+ at concentrations of 1–5 times 10-minus 4 M. Mg2+ at 10-minus 2 M was ineffective. Ghosts were prepared by graded lysis in various buffers. Those prepared in phosphate were the most stable and provided consistent EDTA effects and Ca2+ reversal. Ghosts in Tris-HCl showed breakdown unless salt was added. Moderately satisfactory ghosts were also obtained in Hepes-NaOH buffer and salt.

Download Full-text